Methods for correction of selectivity of N-(2-sulfoethyl)chitosan-based materials towards platinum(IV) and palladium(II) ions

In this paper, we report methods for correction of selectivity of sorbents based on N-(2-sulfoethyl)chitosan towards platinum(IV) and palladium(II) in HCl solutions. The common method for correction of selectivity of the sorbents is variation of their modification degree with complexing groups. An increase in the degree of sulfoethylation of the chitosan leads to the significant increase in selectivity of sorption of palladium(II) over platinum(IV). Application of the N-(2-sulfoethyl)chitosan with the highest degree of sulfoethylation allows for selective separation palladium(II) from platinum(IV) (рН = 5.0). Palladium is quantitatively desorbed from the surface of the N-(2-sulfoethyl)chitosans by 3.5 mol/dm³ solution of HCl.     

Supramolecular Amphiphiles Based on Pillar[5]arene and Meroterpenoids: Synthesis,Self-Association and Interaction with Floxuridine

In recent years, meroterpenoids have found wide biomedical application due to their synthetic availability, low toxicity, and biocompatibility. However, these compounds are not used in targeted drug delivery systems due to their high affinity for cell membranes, both healthy and in cancer cells. Using the approach of creating supramolecular amphiphiles, we have developed self-assembling systems based on water-soluble pillar[5]arene and synthetic meroterpenoids containing geraniol, myrtenol, farnesol, and phytol fragments. The resulting systems can be used as universal drug delivery systems. It was shown by turbidimetry that the obtained pillar[5]arene/synthetic meroterpenoid systems do not interact with the model cell membrane at pH = 7.4, but the associates are destroyed at pH = 4.1. In this case, the synthetic meroterpenoid is incorporated into the lipid bilayer of the model membrane. The characteristics of supramolecular self-assembly, association constants and stoichiometry of the most stable pillar[5]arene/synthetic meroterpenoid complexes were established by UV-vis spectroscopy and dynamic light scattering (DLS). It was shown that supramolecular amphiphiles based on pillar[5]arene/synthetic meroterpenoid systems form monodisperse associates in a wide range of concentrations. The inclusion of the antitumor drug 5-fluoro-2′-deoxyuridine (floxuridine) into the structure of the supramolecular associate was demonstrated by DLS, 19F, 2D DOSY NMR spectroscopy.     

New water-soluble copolymers of 2-methacryloyloxyethyl phosphorylcholine for surface modification

New copolymers of 2-methacryloyloxyethyl phosphorylcholine with 2-Deoxy-2-methacrylamido-D-glucose, N-vinylpyrrolidone, and N-vinyl-N-methylacetamide were synthesized and characterized; the obtained copolymers contained 10–50 mol% of phosphorylcholine moieties, and their molecular masses ranged from 1.05 × 10⁵ to 4.40 × 10⁵. Reactivity ratios of the monomers were estimated. Conformational states of the copolymers in aqueous solutions were studied. The synthesized copolymer was grafted onto the surface of carbon fiber biosorbent using γ-radiation.     

Heteroalleles in Common Wheat: Multiple Differences between Allelic Variants of the Gli-B1 Locus

The Gli-B1-encoded γ-gliadins and non-coding γ-gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major γ-gliadin, restriction fragment length polymorphism patterns (RFLPs) (three different markers), Gli-B1-γ-gliadin-pseudogene known SNP markers (Single nucleotide polymorphisms) and sequencing the pseudogene GAG56B. It was discovered that encoded γ-gliadins, with contrasting EM, had similar MWs. However, seven allelic variants (designated from I to VII) differed among them in the other six tests: I (alleles Gli-B1i, k, m, o), II (Gli-B1n, q, s), III (Gli-B1b), IV (Gli-B1e, f, g), V (Gli-B1h), VI (Gli-B1d) and VII (Gli-B1a). Allele Gli-B1c (variant VIII) was identical to the alleles from group IV in four of the tests. Some tests might show a fine difference between alleles belonging to the same variant. Our results attest in favor of the independent origin of at least seven variants at the Gli-B1 locus that might originate from deeply diverged genotypes of the donor(s) of the B genome in hexaploid wheat and therefore might be called “heteroallelic”. The donor’s particularities at the Gli-B1 locus might be conserved since that time and decisively contribute to the current high genetic diversity of common wheat.     

Distribution of vanadium and vanadyl porphyrins during fractionation of resins of heavy sulfurous oils

Features of the content and distribution of vanadium and vanadyl porphyrins in the fractions of resins, which were obtained by separation using column chromatography on silica, have been studied on heavy sulfurous oils of various deposits of the Volga–Ural basin (Russia). The difference of the vanadium and vanadyl porphyrin contents in the fractions of oils of various production complexes has been shown. Features of structural–group composition and predominant types of vanadyl porphyrins in the fractions of resins have been determined.     

An online color naming experiment in Russian using Munsell color samples

Russian color naming was explored in a web‐based experiment. The purpose was 3‐fold: to examine (1) CIELAB coordinates of centroids for 12 Russian basic color terms (BCTs), including 2 Russian terms for “blue”, sinij “dark blue”, and goluboj “light blue”, and compare these with coordinates for the 11 English BCTs obtained in earlier studies; (2) frequent nonBCTs; and (3) gender differences in color naming. Native Russian speakers participated in the experiment using an unconstrained color‐naming method. Each participant named 20 colors, selected from 600 colors densely sampling the Munsell Color Solid. Color names and response times of typing onset were registered. Several deviations between centroids of the Russian and English BCTs were found. The 2 “Russian blues”, as expected, divided the BLUE area along the lightness dimension; their centroids deviated from a centroid of English blue. Further minor departures were found between centroids of Russian and English counterparts of “brown” and “red”. The Russian color inventory confirmed the linguistic refinement of the PURPLE area, with high frequencies of nonBCTs. In addition, Russian speakers revealed elaborated naming strategies and use of a rich inventory of nonBCTs. Elicitation frequencies of the 12 BCTs were comparable for both genders; however, linguistic segmentation of color space, employing a synthetic observer, revealed gender differences in naming colors, with more refined naming of the “warm” colors from females. We conclude that, along with universal perceptual factors, that govern categorical partition of color space, Russian speakers’ color naming reflects language‐specific factors, supporting the weak relativity hypothesis.     

Sorption isotherms of metal ions onto an N-(2-sulfoethyl)chitosan-based material from single- and multi-component solutions

The scope of work is to study the mutual influence of metal ions during their sorption by sulfoethylated chitosan. The sorption isotherms of metal ions from single- and multi-component solutions are obtained. The sorption capacity of the sorbent towards Ag(I) and Cu(II) is revealed to be 1.63 and 1.41 mmol/g in single-, and 1.40 and 0.85 mmol/g in five-component solution. By comparing the affinity parameter and capacity of sulfoethylated chitosan towards ions in single- and multi-component solutions, it is concluded that Ag(I) and Cu(II) ions suppress the sorption of cobalt(II), nickel(II), zinc(II), cadmium(II), magnesium(II), calcium(II), strontium(II), barium(II), manganese(II) and lead(II).     

天然和热变性乳铁蛋白与α-乳白蛋白聚集体的超分子结构表征

本文在pH6.5磷酸盐缓冲溶液中研究了热变性前后牛乳铁蛋白(bovine lactoferrin,LF)和α-乳白蛋白(α-lactalbumin,ALA)之间的自组装行为。采用ζ-电位、浊度法、动态光散射、光学显微镜、荧光光谱和红外色谱等方法进行表征。结果表明,与天然LF和ALA自组装复合物相比,热变性LF和ALA自组装复合物的ζ-电位较低。天然LF与ALA可以自组装可形成纳米颗粒,粒径最大为(35.24±0.82) nm;经过热变性的LF与ALA自组装形成超分子结构,通过调整ALA浓度,可以得到亚微米和微米颗粒,颗粒粒径最大为(4.11±0.14) μm。天然LF与ALA的复合物均为球状聚集体,分布均一;而热变性LF与ALA的复合物则为网状聚集体,分布不均一。ALA的添加增强了LF中色氨酸残基的疏水性。LF中的C=O和C-N基团均参与了与ALA_{25 ℃}的相互作用。本研究为构建新型双蛋白自组装体及理解双蛋白组装机制提供了理论依据。