全文获取类型
收费全文 | 40113篇 |
免费 | 2525篇 |
国内免费 | 32篇 |
学科分类
工业技术 | 42670篇 |
出版年
2023年 | 360篇 |
2022年 | 347篇 |
2021年 | 995篇 |
2020年 | 840篇 |
2019年 | 943篇 |
2018年 | 1598篇 |
2017年 | 1574篇 |
2016年 | 1678篇 |
2015年 | 1246篇 |
2014年 | 1589篇 |
2013年 | 3168篇 |
2012年 | 2306篇 |
2011年 | 2218篇 |
2010年 | 1793篇 |
2009年 | 1641篇 |
2008年 | 1586篇 |
2007年 | 1455篇 |
2006年 | 1040篇 |
2005年 | 856篇 |
2004年 | 822篇 |
2003年 | 718篇 |
2002年 | 714篇 |
2001年 | 502篇 |
2000年 | 501篇 |
1999年 | 529篇 |
1998年 | 2507篇 |
1997年 | 1757篇 |
1996年 | 1137篇 |
1995年 | 693篇 |
1994年 | 553篇 |
1993年 | 632篇 |
1992年 | 261篇 |
1991年 | 248篇 |
1990年 | 194篇 |
1989年 | 205篇 |
1988年 | 187篇 |
1987年 | 178篇 |
1986年 | 164篇 |
1985年 | 201篇 |
1984年 | 181篇 |
1983年 | 143篇 |
1982年 | 176篇 |
1981年 | 187篇 |
1980年 | 191篇 |
1979年 | 118篇 |
1978年 | 104篇 |
1977年 | 317篇 |
1976年 | 670篇 |
1975年 | 86篇 |
1973年 | 102篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
71.
Zdeněk Slanina Filip Uhlík Lipiao Bao Takeshi Akasaka Xing Lu Ludwik Adamowicz 《Fullerenes, Nanotubes and Carbon Nanostructures》2020,28(7):565-570
AbstractRelative populations of four energy-lowest IPR (isolated-pentagon-rule) isomers of Eu@C86 are computed using the Gibbs energy based on characteristics from density functional theory calculations (M06-2X/3-21G?~?SDD entropy term, M06-2X/6-31G*~SDD or B2PLYP(D)/6-31G*~SDD energetics). The calculations confirm that the recently isolated Eu@C1(7)-C86 species is a major isomer in a relevant temperature region. Relationship to the empty C86 cages is discussed, too. 相似文献
72.
73.
The fission yeast Schizosaccharomyces pombe is an important model organism for the study of fundamental questions in eukaryotic cell and molecular biology. A plethora of cellular processes are membrane associated and/or dependent on the proper functioning of cellular membranes. Phospholipids are not only the basic building blocks of cellular membranes; they also serve as precursors to numerous signaling molecules. In this review, we describe the biosynthetic pathways leading to major S. pombe phospholipids, how these pathways are regulated, and what is known about degradation and turnover of fission yeast phospholipids. This review also addresses the synthesis, regulation and the role of water-soluble phospholipid precursors. The last chapter of the review is devoted to the use of S. pombe for the biotechnological production of value-added lipid molecules. 相似文献
74.
75.
Ricardo Martínez-Corona Gerardo Vázquez Marrufo Carlos Cortés Penagos Luis Alberto Madrigal-Pérez Juan Carlos González-Hernández 《Yeast (Chichester, England)》2020,37(1):149-162
Lipases are hydrolytic enzymes that break the ester bonds of triglycerides, generating free fatty acids and glycerol. Extracellular lipase activity has been reported for the nonconventional yeast Kluyveromyces marxianus, grown in olive oil as a substrate, and the presence of at least eight putative lipases has been detected in its genome. However, to date, there is no experimental evidence on the physiological role of the putative lipases nor their structural and catalytic properties. In this study, a bioinformatic analysis of the genes of the putative lipases from K. marxianus L-2029 was performed, particularly identifying and characterizing the extracellular expected enzymes, due to their biotechnological relevance. The amino acid sequence of 10 putative lipases, obtained by in silico translation, ranged between 389 and 773 amino acids. Two of the analysed putative proteins showed a signal peptide, 25 and 33 amino acids long for KmYJR107Wp and KmLIP3p, and a molecular weight of 44.53 and 58.23 kDa, respectively. The amino acid alignment of KmLIP3p and KmYJR107Wp with the crystallized lipases from a patatin and the YlLip2 lipase from Yarrowia lipolytica, respectively, revealed the presence of the hydrolase characteristic motifs. From the 3D models of putative extracellular K. marxianus L-2029 lipases, the conserved pentapeptide of each was determined, being GTSMG for KmLIP3p and GHSLG for KmYJR107Wp; besides, the genes of these two enzymes (LIP3 and YJR107W) are apparently regulated by oleate response elements. The phylogenetic analysis of all K. marxianus lipases revealed evolutionary affinities with lipases from abH15.03, abH23.01, and abH23.02 families. 相似文献
76.
77.
Alfredo M. Gravagnuolo Eden Morales‐Narváez Charlene Regina Santos Matos Sara Longobardi Paola Giardina Arben Merkoçi 《Advanced functional materials》2015,25(38):6084-6092
Class I hydrophobin Vmh2, a peculiar surface active and versatile fungal protein, is known to self‐assemble into chemically stable amphiphilic films, to be able to change wettability of surfaces, and to strongly adsorb other proteins. Herein, a fast, highly homogeneous and efficient glass functionalization by spontaneous self‐assembling of Vmh2 at liquid–solid interfaces is achieved (in 2 min). The Vmh2‐coated glass slides are proven to immobilize not only proteins but also nanomaterials such as graphene oxide (GO) and quantum dots (QDs). As models, bovine serum albumin labeled with Alexa 555 fluorophore, anti‐immunoglobulin G antibodies, and cadmium telluride QDs are patterned in a microarray fashion in order to demonstrate functionality, reproducibility, and versatility of the proposed substrate. Additionally, a GO layer is effectively and homogeneously self‐assembled onto the studied functionalized surface. This approach offers a quick and simple alternative to immobilize nanomaterials and proteins, which is appealing for new bioanalytical and nanobioenabled applications. 相似文献
78.
79.
In this study, the relationship between stunning techniques and protein oxidation which are accepted as the main cause of food spoilage was investigated. For this purpose, the antioxidant status, in vivo myofibrillary protein (MP) oxidation and sensitivity, and postmortem oxidation (inducted with hydroxyl radical system) of rainbow trout (Oncorhynchus mykiss) fillets killed by hitting to head (T1), neck crushing (T2), and convulsion (T3) methods, were investigated. Statistically significant differences (p < .05) were found among all parameters examined and it was observed that the most stressing technique was the convulsion method. It was determined that in protein profiles myosin were influenced too much from stunning technique and in actin observed oxidation-induced reductions. Reductions in S-S and S-H were also found to be increased in carbonyl concentrations, but the most effective values in both processes were determined by the convulsive technique. Our results show that short-term stunning techniques (hitting to head, neck crushing) give better meat quality results in terms of O. mykiss welfare and low MP oxidation rates. In general, we can say that T3 group fillets are more sensitive to oxidative damage, while T1 and T2 groups give better results in maintaining meat quality with low MP oxidation rates. 相似文献