MicroRNA Profiling of B Cell Subsets from Systemic Lupus Erythematosus Patients Reveals Promising Novel Biomarkers

MicroRNAs control the differentiation and function of B cells, which are considered key elements in the pathogenesis of systemic lupus erythematosus (SLE). However, a common micro(mi)RNA signature has not emerged since published data includes patients of variable ethnic background, type of disease, and organ involvement, as well as heterogeneous cell populations. Here, we aimed at identifying a miRNA signature of purified B cells from renal and non-renal severe SLE patients of Latin American background, a population known to express severe disease. Genome-wide miRNA expression analyses were performed on naive and memory B cells and revealed two categories of miRNA signatures. The first signature represents B cell subset-specific miRNAs deregulated in SLE: 11 and six miRNAs discriminating naive and memory B cells of SLE patients from healthy controls (HC), respectively. Whether the miRNA was up or down-regulated in memory B cells as compared with naive B cells in HC, this difference was abolished in SLE patients, and vice versa. The second signature identifies six miRNAs associated with specific pathologic features affecting renal outcome, providing a further understanding for SLE pathogenesis. Overall, the present work provided promising biomarkers in molecular diagnostics for disease severity as well as potential new targets for therapeutic intervention in SLE.     

Multivariate Analysis of Multiple Datasets: a Practical Guide for Chemical Ecology

Chemical ecology has strong links with metabolomics, the large-scale study of all metabolites detectable in a biological sample. Consequently, chemical ecologists are often challenged by the statistical analyses of such large datasets. This holds especially true when the purpose is to integrate multiple datasets to obtain a holistic view and a better understanding of a biological system under study. The present article provides a comprehensive resource to analyze such complex datasets using multivariate methods. It starts from the necessary pre-treatment of data including data transformations and distance calculations, to the application of both gold standard and novel multivariate methods for the integration of different omics data. We illustrate the process of analysis along with detailed results interpretations for six issues representative of the different types of biological questions encountered by chemical ecologists. We provide the necessary knowledge and tools with reproducible R codes and chemical-ecological datasets to practice and teach multivariate methods.     

The Arabidopsis Mitochondrial Glutaredoxin GRXS15 Provides [2Fe-2S] Clusters for ISCA-Mediated [4Fe-4S] Cluster Maturation

Iron-sulfur (Fe-S) proteins are crucial for many cellular functions, particularly those involving electron transfer and metabolic reactions. An essential monothiol glutaredoxin GRXS15 plays a key role in the maturation of plant mitochondrial Fe-S proteins. However, its specific molecular function is not clear, and may be different from that of the better characterized yeast and human orthologs, based on known properties. Hence, we report here a detailed characterization of the interactions between Arabidopsis thaliana GRXS15 and ISCA proteins using both in vivo and in vitro approaches. Yeast two-hybrid and bimolecular fluorescence complementation experiments demonstrated that GRXS15 interacts with each of the three plant mitochondrial ISCA1a/1b/2 proteins. UV-visible absorption/CD and resonance Raman spectroscopy demonstrated that coexpression of ISCA1a and ISCA2 resulted in samples with one [2Fe-2S]²⁺ cluster per ISCA1a/2 heterodimer, but cluster reconstitution using as-purified [2Fe-2S]-ISCA1a/2 resulted in a [4Fe-4S]²⁺ cluster-bound ISCA1a/2 heterodimer. Cluster transfer reactions monitored by UV-visible absorption and CD spectroscopy demonstrated that [2Fe-2S]-GRXS15 mediates [2Fe-2S]²⁺ cluster assembly on mitochondrial ferredoxin and [4Fe-4S]²⁺ cluster assembly on the ISCA1a/2 heterodimer in the presence of excess glutathione. This suggests that ISCA1a/2 is an assembler of [4Fe-4S]²⁺ clusters, via two-electron reductive coupling of two [2Fe-2S]²⁺ clusters. Overall, the results provide new insights into the roles of GRXS15 and ISCA1a/2 in effecting [2Fe-2S]²⁺ to [4Fe-4S]²⁺ cluster conversions for the maturation of client [4Fe-4S] cluster-containing proteins in plants.     

Intracellular Fate of Hydrophobic Nanocrystal Self‐Assemblies in Tumor Cells

Control of interactions between nanomaterials and cells remains a biomedical challenge. A strategy is proposed to modulate the intralysosomal distribution of nanoparticles through the design of 3D suprastructures built by hydrophilic nanocrystals (NCs) coated with alkyl chains. The intracellular fate of two water‐dispersible architectures of self‐assembled hydrophobic magnetic NCs: hollow deformable shells (colloidosomes) or solid fcc particles (supraballs) is compared. These two self‐assemblies display increased cellular uptake by tumor cells compared to dispersions of the water‐soluble NC building blocks. Moreover, the self‐assembly structures increase the NCs density in lysosomes and close to the lysosome membrane. Importantly, the structural organization of NCs in colloidosomes and supraballs are maintained in lysosomes up to 8 days after internalization, whereas initially dispersed hydrophilic NCs are randomly aggregated. Supraballs and colloidosomes are differently sensed by cells due to their different architectures and mechanical properties. Flexible and soft colloidosomes deform and spread along the biological membranes. In contrast, the more rigid supraballs remain spherical. By subjecting the internalized suprastructures to a magnetic field, they both align and form long chains. Overall, it is highlighted that the mechanical and topological properties of the self‐assemblies direct their intracellular fate allowing the control intralysosomal density, ordering, and localization of NCs.     

Laser sintering of coated polyamide 12: a new way to improve flammability

Journal of Materials Science - In this study coatings of kaolin and talc particles were successfully applied on the surface of polyamide 12 powder intended for laser sintering (LS). Microscopic...     

On the modification of photocatalysts for improving visible light and UV degradation of gas-phase toluene over TiO2

The photocatalytic behavior of different TiO₂-based photocatalysts was reported for gas-phase toluene removal under both UV and visible light illumination, and compared to that of commercial P25 (Degussa) TiO₂. Promotion by sulfates and the use of nanosized anatase TiO₂ were reported to strongly increase the toluene removal efficiency under UV illumination. Nanosized-anatase was prepared by a protecting group sol–gel synthesis using hexamethyldisilazane as crystallite growth inhibitor. Sulfates played a double positive role, with photogenerated electrons transfer effects limiting charge recombination and as repulsive species for strongly adsorbed aromatic intermediates that act as poisons. The decrease in particle size obtained on nanosized anatase TiO₂ (5 nm) yielded a considerable enhancement in the toluene removal efficiency. Pure high surface area rutile has been synthesized at low temperature by a polyethylenglycol-containing sol–gel method for visible light activation purposes. A two-way semiconductor coupling phenomenon, consisting of a reciprocal electron/hole transfer between two visible light-activated oxides, rutile TiO₂ and WO₃, was proposed to explain the large gain in efficiency when adding low amounts of WO₃ to rutile TiO₂.     

Hardware Implementation of Enhancement Embedded Reduced Runtime Recovery Algorithm for Compressively Sensed Images

Compressed sensing is based on the principle that, through optimization, the sparsity of a signal can be exploited to recover it from fewer samples than required by the criterion. In compressed sensing, data is compressed and converted into fewer measurements and transmitted through wireless channel which is reconstructed at the receiver. Since very few samples are used for reconstruction, there are possibilities of degradation in quality of reconstruction. Unlike traditional methods, enhancement can now be done using the recent technique of compressed sensing by embedding image enhancement techniques like edge detection, histogram, filtering and their combinations with CS recovery procedure. This work proposes such a method by binding the image enhancement techniques along with the compressed sensing process. Filter of Gaussian filter (FGF), a combinational filter proposed in this study enables an increase in PSNR of 1 dB when compared to other filtering techniques besides using least number of measurements and maintaining minimum time consumption. The runtime difference with and without the FGF is ~?3 s, which is affordable even in hardware with minimum specifications. Real time experimentation of embedded enhancement CS was carried out in WINGZ board to prove the feasibility of enhanced recovery process with lower end hardware.     

Key relationship-based determinants of project performance in China

The aim of this paper is to investigate the relationship-based factors that affect performance of general building projects in China. Thirteen performance metrics that may be used to measure the success level of construction projects are defined and categorized into four groups namely cost, schedule, quality and relationship performance. Fourteen risks inherent in relationships and 16 tools expected to facilitate relationship building that may affect project success are identified. Data of different projects were collected in China via a self-administered postal survey. Multiple linear regression models are developed to help explain the variance in different performance metrics. It has been found that ten risks and nine tools have either positive or negative influence on project performance to some different extents and in different project development process stages. Detailed explanations are made, especially to those variables bearing unexpected signs. It is recommended that firms in the Chinese construction industry manage the relationship-based factors that are significant in the MLR models so as to achieve project success.     

Toxicity of lipid-soluble copper(II) complexes to the marine diatom Nitzschia closterium: Amelioration by humic substances

Algal assays, using the marine diatom Nitzschia closterium, have established that humic acid (5 mg kg⁻¹) can ameliorate the toxicity of the lipophilic complex Cu(oxine)₂ (3 × 10⁻⁸ mol l⁻¹ in unsupplemented seawater). The toxicity of Cu(PAN)₂ is not ameliorated [PAN = 1-(2-pyridylazo)-2-napthol]. In conjunction with previous visible absorption spectrophotometry and polarographic measurements it was established that humic acid sequesters copper(II) from the hydrophobic complexes, releasing a ligand molecule. The copper(II) toxicity may be ameliorated provided the ligand itself is not toxic.Fulvic acid was significantly less effective in ameliorating toxicity. Because of the significant competition from Ca(II) and Mg(II) in seawater, it is inferred that humic substances may be more effective in ameliorating toxicity of hydrophobic copper complexes in fresh water.