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81.
Luccas M. Barata Eloísa H. Andrade Alessandra R. Ramos Oriel F. de Lemos William N. Setzer Kendall G. Byler Jos Guilherme S. Maia Joyce Kelly R. da Silva 《International journal of molecular sciences》2021,22(2)
This study evaluated the chemical compositions of the leaves and fruits of eight black pepper cultivars cultivated in Pará State (Amazon, Brazil). Hydrodistillation and gas chromatography–mass spectrometry were employed to extract and analyze the volatile compounds, respectively. Sesquiterpene hydrocarbons were predominant (58.5–90.9%) in the cultivars “Cingapura”, “Equador”, “Guajarina”, “Iaçará”, and “Kottanadan”, and “Bragantina”, “Clonada”, and “Uthirankota” displayed oxygenated sesquiterpenoids (50.6–75.0%). The multivariate statistical analysis applied using volatile composition grouped the samples into four groups: γ-Elemene, curzerene, and δ-elemene (“Equador”/“Guajarina”, I); δ-elemene (“Iaçará”/“Kottanadan”/“Cingapura”, II); elemol (“Clonada”/“Uthirankota”, III) and α-muurolol, bicyclogermacrene, and cubebol (“Bragantina”, IV). The major compounds in all fruit samples were monoterpene hydrocarbons such as α-pinene, β-pinene, and limonene. Among the cultivar leaves, phenolics content (44.75–140.53 mg GAE·g−1 FW), the enzymatic activity of phenylalanine-ammonia lyase (20.19–57.22 µU·mL−1), and carotenoids (0.21–2.31 µg·mL−1) displayed significant variations. Due to black pepper’s susceptibility to Fusarium infection, a molecular docking analysis was carried out on Fusarium protein targets using each cultivar’s volatile components. F. oxysporum endoglucanase was identified as the preferential protein target of the compounds. These results can be used to identify chemical markers related to the susceptibility degree of black pepper cultivars to plant diseases prevalent in Pará State. 相似文献
82.
Luis O. Soto-Rojas Mar Pacheco-Herrero Paola A. Martínez-Gmez B. Berenice Campa-Crdoba Ricardo Aptiga-Prez Marcos M. Villegas-Rojas Charles R. Harrington Fidel de la Cruz Linda Garcs-Ramírez Jos Luna-Muoz 《International journal of molecular sciences》2021,22(4)
Alzheimer’s disease (AD) is the most common neurodegenerative disease worldwide. Histopathologically, AD presents with two hallmarks: neurofibrillary tangles (NFTs), and aggregates of amyloid β peptide (Aβ) both in the brain parenchyma as neuritic plaques, and around blood vessels as cerebral amyloid angiopathy (CAA). According to the vascular hypothesis of AD, vascular risk factors can result in dysregulation of the neurovascular unit (NVU) and hypoxia. Hypoxia may reduce Aβ clearance from the brain and increase its production, leading to both parenchymal and vascular accumulation of Aβ. An increase in Aβ amplifies neuronal dysfunction, NFT formation, and accelerates neurodegeneration, resulting in dementia. In recent decades, therapeutic approaches have attempted to decrease the levels of abnormal Aβ or tau levels in the AD brain. However, several of these approaches have either been associated with an inappropriate immune response triggering inflammation, or have failed to improve cognition. Here, we review the pathogenesis and potential therapeutic targets associated with dysfunction of the NVU in AD. 相似文献
83.
Zhen Zeng Hui Yi Chew Jazmina G. Cruz Graham R. Leggatt James W. Wells 《International journal of molecular sciences》2021,22(6)
T cells play a key role in tumour surveillance, both identifying and eliminating transformed cells. However, as tumours become established they form their own suppressive microenvironments capable of shutting down T cell function, and allowing tumours to persist and grow. To further understand the tumour microenvironment, including the interplay between different immune cells and their role in anti-tumour immune responses, a number of studies from mouse models to clinical trials have been performed. In this review, we examine mechanisms utilized by tumour cells to reduce their visibility to CD8+ Cytotoxic T lymphocytes (CTL), as well as therapeutic strategies trialled to overcome these tumour-evasion mechanisms. Next, we summarize recent advances in approaches to enhance CAR T cell activity and persistence over the past 10 years, including bispecific CAR T cell design and early evidence of efficacy. Lastly, we examine mechanisms of T cell infiltration and tumour regression, and discuss the strengths and weaknesses of different strategies to investigate T cell function in murine tumour models. 相似文献
84.
Sara da Mata Joana Ferreira Inmaculada Nicols Susana Esteves Gonalo Esteves Sofia Lrias Fernanda Silva Adela Saco Daniela Cochicho Mrio Cunha Marta del Pino Jaume Ordi Ana Flix 《International journal of molecular sciences》2021,22(5)
The expression of p16 is a good surrogate of human papillomavirus (HPV) infection in HPV-associated cancers. The significance of p16 expression, HPV genotype and genera in the outcome of patients with HPV-associated cervical cancer (CC) is unclear. Our aim is to ascertain the prognostic significance of these factors. Data from 348 patients (median age: 47.5 years old) with CC, diagnosed in two referral centers, were retrospectively collected. Advanced disease (FIGO2018 IB2-IV) was present in 68% of patients. A single HPV genotype was identified in 82.8% of patients. The most common HPVs were HPV16 (69%) and HPV18 (14%). HPV genera reflected this distribution. HPV16 tumors presented at an earlier stage. P16 was negative in 18 cases (5.2%), 83.3% of which were squamous cell carcinomas. These cases occurred in older patients who tended to have advanced disease. In the univariate analysis, HPV16 (HR: 0.58; p = 0.0198), α-9 genera (HR: 0.37; p = 0.0106) and p16 overexpression (HR: 0.54; p = 0.032) were associated with better survival. HPV16 (HR: 0.63; p = 0.0174) and α-9 genera (HR: 0.57; p = 0.0286) were associated with less relapse. In the multivariate analysis, only the International Federation of Gynecology and Obstetrics (FIGO) stage retained an independent prognostic value. HPV16, α-9 genera and p16 overexpression were associated with better survival, although not as independent prognostic factors. Patients with p16-negative HPV-associated CC were older, presented with advanced disease and had worse prognosis. 相似文献
85.
86.
María Fernndez Alicia de Coo Ins Quintela Eliane García Mrcio Diniz-Freitas Jacobo Limeres Pedro Diz Juan Blanco ngel Carracedo Raquel Cruz 《International journal of molecular sciences》2021,22(12)
Severe periodontitis is prevalent in Down syndrome (DS). This study aimed to identify genetic variations associated with periodontitis in individuals with DS. The study group was distributed into DS patients with periodontitis (n = 50) and DS patients with healthy periodontium (n = 36). All samples were genotyped with the “Axiom Spanish Biobank” array, which contains 757,836 markers. An association analysis at the individual marker level using logistic regression, as well as at the gene level applying the sequence kernel association test (SKAT) was performed. The most significant genes were included in a pathway analysis using the free DAVID software. C12orf74 (rs4315121, p = 9.85 × 10−5, OR = 8.84), LOC101930064 (rs4814890, p = 9.61 × 10−5, OR = 0.13), KBTBD12 (rs1549874, p = 8.27 × 10−5, OR = 0.08), PIWIL1 (rs11060842, p = 7.82 × 10−5, OR = 9.05) and C16orf82 (rs62030877, p = 8.92 × 10−5, OR = 0.14) showed a higher probability in the individual analysis. The analysis at the gene level highlighted PIWIL, MIR9-2, LHCGR, TPR and BCR. At the signaling pathway level, PI3K-Akt, long-term depression and FoxO achieved nominal significance (p = 1.3 × 10−2, p = 5.1 × 10−3, p = 1.2 × 10−2, respectively). In summary, various metabolic pathways are involved in the pathogenesis of periodontitis in DS, including PI3K-Akt, which regulates cell proliferation and inflammatory response. 相似文献
87.
1 INTRODUCTIONTi 6Al 4VisoneofthemostimportantTial loys[1,2 ] .Butthisalloyhasbadformabilityforitshighelasticresilience .Therefore ,hotsizingisimpor tant[36 ] .Asthebaseofhotsizing ,thestudyofstressrelaxationhasimportanttheoreticalvalueandpracticalsignificance .Ontheotherhand ,Ti 6Al 4Visusedasfastenermaterialssometimes .Whenthefastenersworkatthetemperaturehigherthanroomtemperature ,stressrelaxationmayresultsinacci dents .Sohowtopreventthestressrelaxationisveryimportant[7] .Uptonow ,… 相似文献
88.
A. H. Bott J. C. Suita A. G. da Silva P. E. V. de Miranda 《Surface & coatings technology》1995,70(2-3):257-260
1H(,p) 4He scattering cross-sections were determined for the 6.5–13 MeV (incident energy) range, thereby extending the energy range for such data beyond that available in the literature. These values are essential for the further development of hydrogen concentration profiling by the transmission elastic recoil detection technique, of particular interest in the design of surface-engineered hydrogen contamination barriers for steels. 相似文献
89.
M. Baity-Jesi R.A. Baños A. Cruz L.A. Fernandez J.M. Gil-Narvion A. Gordillo-Guerrero D. Iñiguez A. Maiorano F. Mantovani E. Marinari V. Martin-Mayor J. Monforte-Garcia A. Muñoz Sudupe D. Navarro G. Parisi S. Perez-Gaviro M. Pivanti F. Ricci-Tersenghi J.J. Ruiz-Lorenzo S.F. Schifano B. Seoane A. Tarancon R. Tripiccione D. Yllanes 《Computer Physics Communications》2014
This paper describes the architecture, the development and the implementation of Janus II, a new generation application-driven number cruncher optimized for Monte Carlo simulations of spin systems (mainly spin glasses). This domain of computational physics is a recognized grand challenge of high-performance computing: the resources necessary to study in detail theoretical models that can make contact with experimental data are by far beyond those available using commodity computer systems. On the other hand, several specific features of the associated algorithms suggest that unconventional computer architectures–that can be implemented with available electronics technologies–may lead to order of magnitude increases in performance, reducing to acceptable values on human scales the time needed to carry out simulation campaigns that would take centuries on commercially available machines. Janus II is one such machine, recently developed and commissioned, that builds upon and improves on the successful JANUS machine, which has been used for physics since 2008 and is still in operation today. This paper describes in detail the motivations behind the project, the computational requirements, the architecture and the implementation of this new machine and compares its expected performances with those of currently available commercial systems. 相似文献
90.
Helson M. da Costa Valéria D. Ramos Leila L. Y. Visconte Cristina R. G. Furtado 《Polymer Bulletin》2007,58(3):597-610
Summary White rice husk ash (WRHA) and black rice husk ash (BRHA) were incorporated into natural rubber (NR) using a laboratory-sized
two-roll mill. A conventional vulcanization system was used for curing and physical tests of the NR vulcanized involved determining
of tensile and tear resistances. For comparison purposes, precipitated silica (Zeosil-175) and carbon black (N774) were used
too. Using the analysis of variance of single-factor experiments, it can be concluded that: BRHA is non-reinforcing filler
and its use is limited to 20 phr; WRHA is semi-reinforcing filler and the variation of filler loading (0 up to 50 phr) causes
the maximum variation upon tensile strength of NR compounds; and, that although carbon black and silica are reinforcing fillers,
a real reinforcement is reached up to 20 phr for tensile strength. 相似文献