人贲门癌,食管癌细胞端粒酶RNA和端粒酶活性检测

目的：检测人贲门组织、贲门癌组织和食管组织、食管癌组织端粒酶ＲＮＡ组分表达及端粒酶活性，探讨其在贲门癌和食管癌中的作用。方法：采用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）和端粒重复扩增法（ＴＲＡＰ）检测了人贲门组织、贲门癌组织和食管组织、食管癌组织端粒酶ＲＮＡ组分及端粒酶活性。     

预防胸腔及切口感染的研究

食管癌及贲门癌切除术后并发胸腔和切口感染,主要是含菌胃液及食管内容物对术野污染所致。我院自1986至1987年对两组食管癌及贲门癌患者术前采用不同的方法处理,术后取得不同的预防感染效果。 食管癌和贲门癌患者随机分两组。实验组:食管     

食管憩室并食管及贲门癌的X线诊断价值

目的：探讨X线钡餐造影对食管憩室并食管及贲门癌的诊断价值。方法：回顾性分析18例经X线钡餐检查、纤维内镜和手术病理证实的食管憩室并食管、贲门癌的X线表现。结果：18例中,单发憩室15例,多发憩室3例,其中最多1例有5个憩室。3个有液气平面。11例食管憩室位于肿瘤之上,7例憩室与肿瘤重叠。食管癌15例,贲门癌3例。憩室X线表现为囊袋状或尖角状钡影突出,肿瘤处黏膜不规则中断破坏、不规则充盈缺损及龛影形成,管腔狭窄,管壁僵硬,扩张度差,对比剂通过缓慢。结论：X线钡餐检查是诊断食管憩室并食管癌、贲门癌最简单、可靠、安全的方法,并能为内镜提供准确的定位,避免憩室穿孔危险。     

光动力疗法在食管癌治疗中的作用

食管癌是世界范围内常见的恶性肿瘤之一。欧美国家食管癌的病理类型以腺癌为主,而中国以鳞癌为主。中国在食管癌治疗的远期疗效长期处于平台期,可切除的食管癌患者平均5年生存率徘徊在30%左右。光动力疗法在食管癌治疗中因为选择性和安全性较好,尤其适用于食管癌前病变及早期食管癌。中晚期患者,PDT能缩小肿瘤,缓解吞咽困难,提高生活质量。     

支架置入治疗食管癌、贲门癌的临床分析

食管癌、贲门癌所致的食管狭窄严重影响患者的生存质量，导致营养不良及水、电解质紊乱，从而导致衰竭死亡。我院自2005．08—2008．02，对出现梗阻症状又无手术适应证的晚期食管癌、贲门癌和术后复发癌的患者41例，行食管蜾钛记忆合金支架置入治疗，取得良好疗效。     

食管内支架术结合放、化疗治疗中晚期食管癌

我们自 1996年开始对食管恶性肿瘤伴管腔狭窄而造成吞咽困难的 37例患者作食管内支架置入术 ,获得了满意的疗效。资料和方法一、临床资料37例食管狭窄伴发吞咽困难者均系恶性肿瘤所致 ,其中男性 31例 ,女性 6例 ,年龄 6 4～ 73岁 ,放疗后致食管狭窄患者 2 7例 (12例放疗后复发 ) ,食管下段癌术后 2年致吻合口狭窄 1例 ,9例为下段食管癌及胃底贲门癌侵犯下段食管 ,全部病例经食管钡餐检查和纤维胃镜及病理证实。 37例中发生在中上段的中晚期食管癌患者为 15例 (均采用放疗加化疗 ) ,食管下段癌胃底贲门癌发生肝内转移 6例。腹膜后淋巴结转移 …     

探条扩张在光动力疗法治疗食管贲门癌性狭窄中的应用

正目的:评价探条扩张术在光动力疗法(Photodynamic Therapy,PDT)及局部化疗治疗食管贲门癌性狭窄中的疗效。方法:食管贲门癌患者108例,吞咽困难程度参照Stooler分级。按治疗方法分为对照组(PDT联合局部化疗)和治疗组(探条扩张联合PDT及局部化疗)。对照组56例中,食管癌44例,均为鳞癌;贲门癌8例,贲门癌术后局部复发4例,均为腺癌。治疗组52例中,食管癌40例,均为鳞癌;贲     

体表超声对进展期贲门癌的诊断价值

目的:探讨经体表超声判断贲门癌沿食管向上侵犯的长度及浸润深度的准确性,及其在外科治疗方案选择中的价值.方法:经体表超声对40例贲门癌患者探查,重点观察肿瘤侵犯食管长度及是否侵犯浆膜,并与术后病理结果对照.结果:超声诊断肿瘤侵犯外膜的灵敏性为71.0%,特异性为77.8%,总的符合率为72.5%.4例食管没有受到侵犯的患者诊断符合率为100%,36例食管侵犯的患者超声诊断的符合率为78%(28/36).贲门癌沿食管向上侵犯长度超声测量误差为(0.77±0.46) cm.结论:体表超声在贲门癌诊断中发挥重要作用,尤其是能够对受侵犯食管的长度及浸润深度作出判断,对外科治疗方案的选择有着很高的参考价值.     

食管癌贲门癌切除后食管胃隧道吻合术加大网膜覆盖56例

1992—01～2003—03，我们对食管癌、贲门癌切除术后的吻合方法进行了改进，应用食管胃“隧道式”吻合术加胃大网膜包裹覆盖术连续进行56例吻合。治疗食管癌、贲门癌效果满意，无吻合口瘘、吻合口狭窄、手术死亡率。现报告如下。     

食管和贲门癌手术并发症的预防

预防食管和贲门癌手术并发症,仍然是一个重要研究课题。我院自1983年2月至1987年2月连续施行食管和贲门癌手术200例。现据本组经验就如何预防手术并发症提出讨论。 临床资料 一、本组200例,食管癌106例,贲门癌94例。男171例,女29例,年龄最小23岁,最大74岁,平均54岁。 二、手术种类 1.经左胸食管和贲门癌切除,食管胃弓上吻合术84例,弓下吻合术88例。 2.经颈、右胸、上腹食管癌切除,食管胃颈     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

The role of interventional radiology in trauma

Introduction Interventional Radiology has evolved into a specialty having enormous input into the care of the traumatized patient.In all hospitals,regardless of size,the Interventional Radiologist must consider their relationships with the trauma service in order to     

Carbohydrate Supplementation during Exercise: Does It Help? How Much is Too Much?

KEY POINTS· Carbohydrate intake during exercise can delay the onset of fatigue and improve performance of prolonged exercise as well as exercise of shorter duration and greater intensity (e.g., continuous exercise lasting about 1h and intermittent high-intensity exercise), but the mechanisms by which performance is improved are different.     

Diagnóstico ecográfico del neumotórax

The ultrasonographic diagnosis of pneumothorax is based on the analysis of artifacts. It is possible to confirm or rule out pneumothorax by combining the following signs: lung sliding, the A and B lines, and the lung point. One fundamental advantage of lung ultrasonography is its easy access in any critical situation, especially in patients in the intensive care unit. For this reason, chest ultrasonography can be used as an alternative to plain-film X-rays and computed tomography in critical patients and in patients with normal plain films in whom pneumothorax is strongly suspected, as well as to evaluate the extent of the pneumothorax and monitor its evolution.     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

磁共振DWI和动态增强鉴别颅底脊索瘤和侵袭性垂体瘤的应用

目的 探讨磁共振扩散加权成像(DWI)和动态增强在颅底脊索瘤和侵袭性垂体瘤(IPA)鉴别诊断中的应用价值.方法 搜集经手术病理证实且影像学有鞍区破坏的颅底脊索瘤患者15例、向鼻咽部侵犯的IPA患者20例.测量二者的表观扩散系数(ADC)值,绘制受试者工作特征曲线(ROC),分析动态增强曲线的类型,统计达峰时间(TTP)、增强峰值(EP)和最大对比增强率(MCER),分析各个参数在鉴别诊断中的价值.结果 颅底脊索瘤的ADC值为(1.274±0.07)×10-3mm2/s,高于IPA ADC值(0.672±0.03) ×10-3 mm2/s(P ＜0.001),ADC阈值为0.964×10-3mm2/s时,ROC曲线下面积为0.997,敏感度为93.3％,特异度为100％.颅底脊索瘤时间-信号强度曲线(TIC)Ⅰ型14例,TICⅢ型1例,此例TICⅢ型者TTP约40 s;IPA TIC Ⅰ型7例,TICⅢ型13例.颅底脊索瘤和IPA的EP、MCER差异均有统计学意义(P ＜0.001).结论 ADC值和TIC的类型及其相关参数(EP,MCER)有助于颅底脊索瘤和IPA之间的鉴别.     

Treatment of intrahepatic arteriovenous fistula in hepatocellular carcinoma with N-butyl-2-cyanoacrylate

Primary hepatocellular carcinoma (HCC) continues to be one of the most common malignancies with an incidence of approximately one million cases per year and a dismal prognosis; some authors have reported a median survival of 1 ～ 2 months after diagnosis. Although surgery remains the only hope for cure, few patients are candidates[1,2].     

U50,488H对正常及缺氧心肌细胞L型钙电流的作用

目的 探讨心脏阿片受体和β-受体相互作用的机制。方法 采用全细胞膜片钳技术,观察U50,488H(β-阿片受体选择性激动剂)对正常和缺氧心肌细胞L型钙电流的作用。结果 U50,488H剂量依赖性(0．1～100μmol／L)抑制正常心肌细胞的L型钙电流及异丙肾上腺素(0．1μmol／L)激动的钙电流,而细胞缺氧后,这一抑制作用减弱;U50,488H对Forskolin(10μmol／L)激动的L型钙电流无明显影响。结论 β-阿片受体对β-受体信号的负性调节作用在细胞缺氧后减弱,其作用位点可能发生于β-受体与腺苷酸环化酶环节之间。     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.