滋养层细胞表面抗原2在鼻咽癌中的表达

目的探究滋养层细胞表面抗原2(Trop-2)在鼻咽癌中的表达情况。方法收集2016年10月-2017年12月东莞市人民医院耳鼻喉科行鼻咽活检的组织标本60份:包括40份鼻咽部未分化非角化型鼻咽癌组织和20份慢性鼻咽炎组织。采用免疫组化法检测Trop-2蛋白,并分析该蛋白在鼻咽癌不同临床分期中的表达情况。结果鼻咽炎患者组织中未出现Trop-2蛋白表达,而在鼻咽癌组织中均有Trop-2蛋白表达,且随着临床分期的增加临床强度增加,各组之间差异有统计学意义(P0.05);在鼻咽炎组织中未出现Trop-2阳性,而在鼻咽癌组织中随着临床分期的增加,Trop-2阳性逐渐从基层侵袭表皮全层,最后侵入到间质部位。结论 Trop-2可以作为发生鼻咽部未分化非角化型鼻咽癌患者的早期诊断指标,具有较好的鼻咽癌临床诊断价值。     

Galectin-3蛋白在鼻咽癌组织的表达及其临床意义

目的 探讨Galectin-3蛋白在鼻咽癌组织的表达及其临床意义.方法 采用免疫组化的方法检测Galectin-3蛋白在94例鼻咽癌和50例慢性鼻咽炎组织中的表达,分析Galectin-3蛋白的表达差异,以及其与临床病理参数和复发转移的关系.结果 鼻咽癌患者肿瘤组织中的Galectin-3阳性表达率显著高于慢性鼻咽炎患...     

AnnexinⅠ在鼻咽癌中的表达及意义

目的：AnnexinⅠ是钙磷脂结合蛋白家族的一员,探讨AnnexinⅠ在慢性鼻咽炎、鼻咽癌组织中的表达及与鼻咽癌多项临床病理特征的关系。方法：采用免疫组织化学SABC法检测Annexin I在慢性鼻咽炎、鼻咽癌组织中的表达。结果：①Annexin I在鼻咽癌组织中的阳性表达率为29.55%,明显低于其在慢性鼻咽炎组织中的阳性表达率93.33%,差异具有显著性（P〈0.01）。②Annexin Ⅰ的表达与鼻咽癌的组织分化程度有关（P〈0.01）,与临床分期、颅神经侵犯、颈淋巴结转移无相关性（P〉0.05）。结论：AnnexinⅠ在鼻咽癌中的表达明显下调,鼻咽癌中Annexin I的表达与组织分化程度密切相关,Annexin I可能与鼻咽癌的发生发展有关。     

基质金属蛋白酶-7在鼻咽癌中的表达及与肿瘤侵袭转移的关系

目的：观察基质金属蛋白酶-7（MMP-7）蛋白在鼻咽癌中的表达及与肿瘤侵袭转移的关系。方法：采用成组设计的对照试验方法，运用免疫组化及逆转录聚合酶链式反应（RT-PCR）技术，检测MMP-7蛋白及mRNA在鼻咽癌及慢性鼻咽炎组织中的表达。使用方差分析、q检验、t检验和X^2检验进行组间差异的统计学分析。结果：MMP-7蛋白在鼻咽癌中的阳性表达率为79．17％，明显高于在慢性鼻咽炎中的表达率11．11％（P〈0．001）；MMP-7mRNA在鼻咽癌中的阳性表达率为89．58％，表达量为0．467±0．199，显著高于在慢性鼻咽炎组织中的表达率16．67％（P〈0．001）；MMP-7蛋白及mRNA的表达与鼻咽癌患者的性别、年龄无关，与鼻咽癌的T分期、N分期及临床分期密切相关（P〈0.05）。结论：MMP-7在鼻咽癌中阳性表达率高，其表达随着肿瘤浸润深度、区域淋巴结转移范围和临床分期的增加而增强，与鼻咽癌侵袭及转移密切相关。     

鼻咽癌组织中凋亡相关基因蛋白表达及其意义

目的:治疗前随机抽取鼻咽癌和慢性鼻咽炎组织中的bcl-2及bax蛋白的表达进行观察和比较,探讨两者在鼻咽癌及慢性鼻咽炎组织中的表达和相互关系。方法:对36例鼻咽癌和26例慢性鼻咽炎标本进行bcl-2和bax的免疫组织化学染色,并进行统计学分析。结果:bcl-2和bax表达水平在鼻咽癌和慢性鼻咽炎组织间差异有显著性(P<0.05)。bcl-2蛋白在鼻咽癌Ⅰ~Ⅱ期的表达比Ⅲ~Ⅳ期高,随分期增高而下降,组间差异显著(P<0.05);bax表达随分期增高而增高,组间差异不显著(P>0.05)。在鼻咽癌中,bcl-2阳性率比bax高,两者表达呈显著负相关(P<0.05)。结论:bcl-2与bax相互作用在鼻咽癌的发生和发展中起重要作用。     

Ezrin和E-cadherin在鼻咽癌组织中的表达及意义

目的：检测鼻咽癌组织中埃兹蛋白(Ezrin)及上皮细胞钙黏蛋白(E-cadherin)的表达情况,探讨它们与肿瘤侵袭、转移及预后的关系。方法：应用免疫组织化学SP法检测Ezrin及E-cadherin蛋白在42例鼻咽癌组织和10例鼻咽炎性组织中的表达情况。结果：鼻咽癌组织中Ezrin 蛋白的表达显著高于鼻咽炎性组织（P＜0.05）,而鼻咽癌组织中E-cadherin蛋白的表达显著低于鼻咽炎性组织（P＜0.05）。Ezrin和E-cadherin蛋白在鼻咽癌组织中的表达均与鼻咽癌的T分期、颈淋巴结转移、临床分期密切相关（P＜0.05）。Ezrin与E-cadherin在鼻咽癌组织中的表达呈明显负相关(r=-0.450,P＜0.05);生存分析显示Ezrin阳性表达、肿瘤TNM分期、E-cadherin表达下调及有淋巴结转移者与鼻咽癌患者的生存率有关（P＜0.05）。结论：Ezrin和E-cadherin在鼻咽癌组织中的表达呈明显的负相关。Ezrin和E-cadherin与鼻咽癌临床分期、淋巴结转移有关,有可能作为鼻咽癌的重要肿瘤标志物。联合检测Ezrin和E-cadherin的表达水平,可能为鼻咽癌预后判断提供依据。     

Skp2与P27~(kip1)在鼻咽癌组织中的表达及意义

目的:F-box蛋白家族中的一员Skp2参与细胞周期抑制蛋白P27kip1泛素化降解,研究发现Skp2在乳腺癌、胃癌、前列腺癌等多种恶性肿瘤中表达增加。本研究旨在探讨Skp2与P27kip1在鼻咽癌组织中的表达及与鼻咽癌多项临床病理特征的关系,并探讨两者的相关性。方法:鼻咽癌组39例,慢性鼻咽炎组24例。标本均经石蜡包埋,HE染色确诊。Skp2和P27kip1蛋白的检测采用免疫组化染色(PV-9000二步法)。结果:(1)Skp2在鼻咽癌组织中的阳性表达率为71.79%,明显高于其在慢性鼻咽炎组织中的阳性表达率(41.67%),差异有统计学意义(P<0.05);P27kip1在鼻咽癌组织中的阳性表达率为35.90%,明显低于其在慢性鼻咽炎组织中的阳性表达率(79.17%),差异有统计学意义(P<0.01);(2)Skp2、P27kip1的蛋白表达与鼻咽癌的临床分期、颅神经侵犯有关(P<0.05),与年龄、性别、分化程度、颈淋巴结转移无关(P>0.05);(3)Skp2、P27kip1在鼻咽癌组织中的表达呈负相关(rs=-0.373,P=0.019)。结论:鼻咽癌中Skp2蛋白表达与靶蛋白P27kip1蛋白降解有关,Skp2、P27kip1可能与鼻咽癌的发生发展有关,联合检测Skp2、P27kip1蛋白表达可作为判断鼻咽癌生物学行为的指标之一。     

p14ARF、mdm2、p53蛋白在鼻咽癌中表达的研究

目的探讨p14ARF、mdm2、p53蛋白在鼻咽癌中的表达及其意义。方法应用免疫组化法检测58例鼻咽癌组织和20例慢性鼻咽炎症组织中p14ARF、mdm2、p53蛋白的表达情况。结果p14ARF、mdm2、p53蛋白在鼻咽癌组织和慢性鼻咽炎症组织中的阳性表达率分别为31.0%、41.4%、65.5%和100%、0、0,各个蛋白在两组之间表达的差异有统计学意义(P<0.05)。结论p14ARF蛋白低表达以及mdm2、p53蛋白高表达与鼻咽癌有关。     

CDK2在鼻咽癌中的表达及意义

目的探讨细胞周期蛋白依赖性激酶2（CDK2）在鼻咽癌和慢性鼻咽炎黏膜组织中的表达水平,及其与鼻咽癌的临床病理关系。方法免疫组化SP法检测CDK2蛋白在鼻咽癌和慢性鼻咽炎黏膜组织中的表达情况。结果 CDK2蛋白阳性表达主要定位于细胞核,少数有胞浆着色,呈棕黄色或棕褐色不同强度的染色。62例鼻咽癌组织中阳性表达率为69.4%（43/62）,慢性鼻咽炎黏膜组织中阳性率为32.0%（9/28）,两组间差异有统计学意义（P〈0.05）。CDK2蛋白表达与鼻咽癌分化程度,淋巴结转移范围,TNM分期有关（P〈0.05）。结论 CDK2在鼻咽癌组织中的表达明显高于慢性鼻咽炎黏膜组织中的表达,提示CDK2与鼻咽癌的发生、发展有关。CDK2与鼻咽癌分化程度,淋巴结转移范围,TNM分期有关。     

真核细胞翻译起始因子4E蛋白在鼻咽癌中的表达及其临床意义

目的半定量分析真核细胞翻译起始因子4E(eIF4E)在鼻咽癌组织中的表达及其与各临床病理参数的相关性。方法采用SP免疫组化法检测40例鼻咽癌组织和40例癌旁鼻咽黏膜慢性炎组织中eIF4E蛋白的表达情况,使用Imagepro-plus 6.0软件分析eIF4E蛋白表达的阳性程度。结果鼻咽癌组织及鼻咽黏膜慢性炎组织中eIF4E蛋白表达平均光密度值(MOD值)分别为(0.153±0.041)和(0.109±0.037),二者差异具有统计学意义(P<0.05)。鼻咽癌组织临床分期越晚,阳性表达率越高;分化程度越高,阳性表达率越低;eIF4E表达在淋巴结转移组织中明显高于无淋巴结转移组织(P<0.05)。结论 eIF4E与鼻咽癌的进展及生物学行为有密切关系,可作为判断鼻咽癌恶性程度的指标。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.