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目的 探讨肝细胞凋亡在丁型肝炎发病机制中的作用。方法 采用免疫经双重染色和TUNEL技术,检测79例丁肝病人肝组织HDAg和肝细胞凋亡分布及其关系,以54例乙型肝炎病人肝组织作对照。结论 HDAg以肝细胞核表达为主,HDAg、凋亡细胞表达分布与肝组织病理损害程度一致(P〈0.01),多数凋亡细胞位于HDAg阳性部位或附近,肝细胞凋亡在各型肝炎中的分布强度差别有显著性意义(P〈0.01)。结论 HD 相似文献
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妊娠合并急性脂肪肝的诊断和处理 总被引:1,自引:1,他引:1
妊娠期急性脂肪肝与重型肝炎是发生在妊娠晚期或产褥早期的严重而少见的致命性并发症。起病急骤,病情凶险,如对本病的早期症状和体征认识不足,延误诊断,就会造成母婴死亡。本文着重讨论妊娠期急性脂肪肝和重型肝炎的诊断及处理,以改善母儿的预后,获得良好的妊娠结局。 相似文献
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李明 《同济医科大学学报》1993,22(5):302-304
用兔抗人纤维联接蛋白(FN)抗体经PAP法染色,应用形态计量学方法对45例乙型病毒性肝炎患者肝组织内FN的分布进行研究。结果表明,除慢性持续性肝炎组外,各型肝炎组肝小叶内FN含量均有所减少,其程度与肝炎时肝细胞变性、坏死程度相关。然而慢性持续性肝炎组、慢性活动性肝炎组及亚急性重型肝炎组门管区内FN含量与正常肝组比较均有所增多。FN沉积的增多与该区域内纤维结缔组织的增生相平行。 相似文献
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重型病毒性肝炎肝组织内丙型肝炎病毒抗原表达的研究 总被引:3,自引:1,他引:2
目的:研究重型肝炎肝组织内丙型肝炎病毒(HCV)抗原表达及特点,探讨HCV感染在重型肝炎发生中的地位和作用。方法:用免疫组化法检测20例重型肝炎组织内HCV抗原。结果:肝组织内HCV抗原表达阳性率45%(9/20),其中慢性重型肝炎60%(6/10),亚急性42.9%(3/7),急性0%(0/3),HCV抗原仅在肝细胞内表达,可呈胞浆均质型、包涵体型和膜下型,9例肝组织HCV抗原表达阳性患者均混合 相似文献
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为了解重型乙型肝炎时乙型肝炎病毒(HBV)的复制状况,应用酶联免疫吸附实验和聚合酶链反应测定了56例乙型肝炎病人的HBV复制指标。慢性重型肝炎病人的HBeAg和/或HBV-DNAPCR阳性率明显低于非重型慢性肝炎(P<0.01),表明HBV复制活跃不一定是重型肝炎发生的因素之一。进一步证明HBV本身并无致肝细胞损伤作用 相似文献
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使用肝细胞生长素(HGF)治疗重型肝炎26例,并以同期综合治疗的24例作对照。结果表明,治疗组在提高存活率、降低转氨酶、降低胆红素、改善凝血机理方面明显优于对照组(P<0.05)。提示,HGF能促进肝细胞生长,治疗重型肝炎疗效满意。 相似文献
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促肝细胞生长素治疗重型病毒性肝炎34例临床疗效观察 总被引:1,自引:0,他引:1
重型病毒性肝炎病情凶险,并发症多,治疗困难,预后极差,目前临床上主要采用综合治疗方法,但疗效尚不理想。我院于1994年4月至1998年12月应用促肝细胞生长素(PHGF)治疗重型病毒性肝炎34例,取得了较好的效果,现报道如下。1 临床资料1.1 病例选择 62例均系我院1994年4月至1998年12月同期住院患者,随机分为治疗组及对照组。其中治疗组34例,男24例,女10例,年龄18~66岁,急性重型肝炎6例,亚急性重型肝炎8例,慢性重型肝炎20例。对照组28例,男19例,女9例,年龄20~68岁,急性重型肝炎4例,亚急性重型肝炎6例,慢性重型肝炎18例。两组重型肝炎按功能衰竭分期,其中早期:治疗组10例,对照组4例;中期:治疗组14例,对照组5例;晚期:治疗组10例,对照组19例。两组病例均符合1995年5月(北京)第五次全国传染病寄生虫病学术会议修订的诊断标准[1],其中急性重型肝炎(急重肝)、亚急性重型肝炎(亚重肝)及慢性重型肝炎(慢重肝)的肝功能衰竭分期为:①早期:严重的全身及消化道症状,SB≥171μmol/L,凝血酶原活动度(PTA)≤40%;②中期:有Ⅱ度肝性脑病或明显腹水,出血倾向(出血点或瘀... 相似文献
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急性重型肝炎肝细胞坏死及其增殖状况的研究 总被引:1,自引:0,他引:1
急性重型肝炎肝细胞坏死及其增殖状况的研究郎振为,李德芬,金荣华,孟忻我们采用免疫组织化学技术对39例急性重型肝炎肝组织内增殖细胞核抗原(PCNA)进行了检测,以了解急性重型肝炎时肝细胞增殖能力及其增殖状况与预后的关系。一、资料和方法标本选自1983~... 相似文献
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目的:探讨CK7、8、19,白蛋白,甲胎蛋白AFP和CD34在慢性重型肝炎(CSH)患者肝组织内的表达;分析肝内胆管增生和肝再生的关系。方法:12例CSH肝组织行HE和嗜银染色;用SP免疫组织化学染色检测CK7、8、19,白蛋白、AFP和CD34表达。结果:死亡2例诊断为慢性重型肝炎;10例恢复期患者病理诊断为早期肝硬化。肝组织内胆管明显增生。肝组织内典型胆管增生细胞CK7、19强阳性;非典型胆管增生细胞CK8强阳性,CK7、19呈弱阳性,部分同时表达CK19和白蛋白,其细胞形态和表型符合肝卵圆细胞特征。结论:慢性重型肝炎患者肝组织内部分非典型增生胆管细胞可能来源于肝卵圆细胞;慢性重型肝炎患者肝细胞增生可能是干细胞参与的再生过程。 相似文献
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In 75 patients with subacute fulminant hepatitis B (SAFH), HDAg positive liver cells were observed in 14 cases (18.67%) by direct enzyme labelled method. It was found further that HDV (delta factor) infection was an important cause in producing massive necrosis of liver cells and superinfection of HDV with hepatitis B was identified as one of the causes of SAFH. This experiment also showed that the number of HDAg positive cells was in direct proportion to the area of liver necrosis. No obvious lymphocytic infiltrations and cytotoxic phenomena around the necrotic foci and HDAg positive cells could be found, while the cytoplasmic type HDAg positive cells showed distinct degeneration or even atrophy. Hence it was suggested that HDV could be a direct pathogenesis. No obvious characteristic changes in morphology of HDV infection could be found.
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目的 分析影响妊娠合并重型肝炎预后的单因素。方法 对66例妊娠合并重型肝炎患者预后的单因素进行回顾性分析。结果 ①不同临床分型的病死率不同,急性型最高,慢性型最低;②Tch、PTA降低、Scr升高及WBC≥10*109/L、"胆酶分离"现象、肝脏缩小均提示病死率升高;③随着肝性脑病、肝肾综合征、出血、感染等并发症的增多,病死率升高, 以上差异均有统计学意义。结论 临床分型、Tch、PTA、Scr、WBC、"胆酶分离"现象、肝脏缩小及并发症等是影响妊娠合并重型肝炎预后的重要因素,动态监测上述指标,积极防治各类并发症是提高临床救治成功率的重要措施。 相似文献
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A G Sheil G W McCaughan H I Isai F Hawker J F Thompson S F Dorney 《The Medical journal of Australia》1991,154(11):724-728
OBJECTIVES: To report the experience of the Australian National Liver Transplant Unit with patients with fulminant hepatic failure and to describe the role of liver transplantation. PATIENTS: Twenty-seven patients presented with acute or subacute fulminant hepatic failure during the period from January, 1986, to March, 1990. Twenty-two had acute and five had subacute fulminant hepatic failure. The causes were hepatitis B in 10 patients, presumed non-A, non-B (NANB) hepatitis in eight patients, drug-induced hepatic damage in five patients, and Wilson's disease in four patients. There were 13 males and 14 females. Ages were 2-43 years (mean, 23). Twenty patients (74%) were in grade IV encephalopathy on presentation. RESULTS: Six patients (22%) began to improve soon after admission and went on to full recovery. Spontaneous recovery was more frequent in patients with drug-induced hepatic damage (four patients [80%]) and was less frequent in those with hepatitis B (one patient [10%]) and NANB hepatitis (one patient [12%]). The other 21 patients (78%) were considered for orthotopic liver transplantation. Eight (30%) were judged to be unsuitable and went on to early death. Thirteen (48%) were suitable for transplantation. Of these five (19%) died before a liver donor became available and eight (30%) received liver grafts and went on to full recovery. Overall, 14 patients (52%) survived and 13 (48%) died. Patients with Wilson's disease (four [100%]) were most suitable for orthotopic liver transplantation whereas eight (44%) of those with hepatitis B or NANB hepatitis were unsuitable. Of the eight patients receiving liver grafts one had hepatitis B, three had NANB hepatitis and four had Wilson's disease. Five were in grade IV encephalopathy at the time of operation. The mean waiting time for transplantation was 6.4 days. Five patients received ABO blood group compatible grafts and three received ABO incompatible grafts. Of the latter group, two subsequently required secondary orthotopic liver transplantation with ABO compatible grafts. All eight patients who received transplants are alive and well 3-24 months after the operation. No patient has any neurological sequelae. CONCLUSIONS: Orthotopic liver transplantation is a preferred option for patients with fulminant hepatic failure whose condition is not responding to conservative management. ABO incompatible livers transplanted in emergency circumstances may prove life-saving either by functioning successfully or by providing time during which ABO compatible grafts become available.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
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It has been demonstrated that severe liver damage and aggravated clinical manifestations and poorprognosis might take place in AS patients accompanied with hepatitis B[l--SJ. The status of cellular immune response in the liver, however, has not beenavailable in the literatures about the disease up tonow. In this study, T lymphocyte subsets in the liverwere detected by immunohistochemical technique inAS patients and those with AS accompanied withhepatitis B. The distribution of T lymphocyt… 相似文献
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Hepatitis B virus infection in medical and health care personnel 总被引:4,自引:0,他引:4
M E Callender Y S White R Williams 《British medical journal (Clinical research ed.)》1982,284(6312):324-326
Analysis of 51 cases of hepatitis B virus infection in health care workers admitted as patients to the liver unit over seven years showed three healthy carriers of hepatitis B virus, seven cases of fulminant hepatic persistent hepatitis, 17 cases of chronic active hepatitis (of whom 11 had cirrhosis), and five cases of hepatocellular carcinoma. To date 11 of these patients have died. Only 15 of the 51 patients had a history of direct occupational exposure and only three patients could recall specific inoculation injuries. In contrast, the source of infection was apparent in 32 of 50 consecutive cases of fulminant hepatic failure or acute hepatitis B in nonmedical staff. Since specific inoculation injuries are not the usual mode of infection ion medical staff and since only a few of the patients who are hepatitis B virus carriers will be detected by selective screening of "high-risk" patients, the overall risk of infection can be reduced only by stricter precautions in the handling of any patient's blood and by the use of hepatitis B virus vaccines for medical staff at high risk. 相似文献
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慢性乙型肝炎免疫细胞NKG2D表达在肝病重型化中的作用 总被引:2,自引:0,他引:2
目的分析乙型肝炎免疫细胞NKG2D表达与肝病重型化的关系。方法应用免疫荧光技术和流式细胞术(FCM)分选20例慢性重型乙肝(FHB)、10例无症状表面抗原携带者(ASC)、10例健康对照的外周血NK细胞,并定量分析NKG2D表达。应用免疫组化SP法分析20例慢性乙肝(CHB)(轻度8例,其中G1级6例,G2级2例;中度7例,重度5例,其中G3级9例,G4级3例)、11例FHB肝组织内细胞毒细胞NKG2D的表达差异。结果经FACS可获得高纯度、高活性的NK细胞。FHB患者NK细胞的NKG2D蛋白表达量高于ASC和正常对照组,差异有显著性(P〈0.01)。免疫组化结果显示,FHB组肝脏免疫细胞NKG2D蛋白表达量分别高于CHB(G3、G4)组、CHB(G1、G2)组和正常对照组,差异有显著性(P〈0.01)。CHB(G3、G4)组高于正常对照组(P〈0.01),但CHB(G1、G2)组分别与CHB(G3、G4)组和正常对照组比较,差异无显著性(P〉0.05)。结论乙型肝炎外周血NK细胞和肝组织内细胞毒细胞NKG2D过度表达与重型肝炎发生相关。 相似文献
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作者用高效液相色谱法测定28例急性肝炎患者的20种血浆氨基酸值。结果表明:急性肝炎患者17种氨基酸、重症肝炎患者13种氨基酸有显著增多;支链氨基酸(BCAA)/芳香族氨基酸(AAA)克分子比值显著降低。血浆氨基酸增多以蛋氨酸最为显著,在3例胆酶分离的重症肝炎患者蛋氨酸也明显升高,这可以作为反映肝细胞坏死程度的较好指标。讨论了肝炎患者血浆氨基酸代谢紊乱的机理和氨基酸测定的临床意义。 相似文献