参芪扶正注射液对心肌挫伤后家兔心肌细胞凋亡的影响

目的 探讨参芪扶正注射液对心肌挫伤后家兔心肌细胞凋亡及其Bcl-2和Bax蛋白表达的影响.方法 健康家兔50只,简单随机化摸球法分为4组,参考组(Reference,n=20)、对照组(Control,n=10)、参芪扶正注射液治疗组(Sen,n=10)和心肌挫伤组(MC,n=10).利用BIM-Ⅱ型生物撞击机制备心肌挫伤模型,应用ELISA法测定心肌肌钙蛋白Ⅰ(cTnI),应用TUNEL法并结合流式细胞术观察心肌细胞凋亡发生的情况,应用免疫组化SABC法观察Bcl-2和Bax蛋白的表达.对照组除不撞击胸部外,余处理均同MC组.结果 MC组与Sen组在伤后4 h血清cTnI含量均高于各自伤前及对照组相应时相点(P＜0.05);对照组未见TUNEL阳性细胞,MC组和Sen组心肌细胞凋亡指数(AI)分别为(15.686 0±0.688 7)%和(5.579 0±0.191 7)%(P＜0.05),凋亡率分别为(6.932 0±0.638 0)%和(3.021 0±0.254 0)%(P＜0.05);与对照组比较,MC组的Bcl-2蛋白和Bax蛋白表达光密度值均明显增高(P＜0.05);而Sen组的Bcl-2蛋白表达光密度值高于MC组(P＜0.05),Bax蛋白表达光密度值低于MC组(P＜0.05).结论 参芪扶正注射液能有效的减少或抑制钝性心肌挫伤家兔心肌细胞的凋亡,对钝性心肌挫伤具有一定的保护作用,其抑制凋亡可能是通过上调Bcl-2蛋白表达和下调Bax蛋白表达来实现的.     

复苏犬心肌线粒体Ca^2＋ ATP酶活性及Ca^2＋含量的变化和意义

目的探讨复苏犬心肌细胞线粒体Ca2+ATP酶活性及Ca2+含量的变化及其在复苏后心肌损伤中的作用.方法电击致犬室颤,15分钟后进行标准复苏,测量心脏停跳15分钟,复苏后0.5小时、2小时、4小时心肌线粒体Ca2+ATP酶活性及Ca2+离子含量.结果与正常组相比,Ca2+ATP酶活性于复苏后半小时显著下降(P＜0.01),复苏后4小时恢复正常(P＞0.05).线粒体Ca2+含量与正常组相比复苏后半小时明显升高(P＜0.01),2小时达最高,复苏后4小时仍未恢复(P＜0.01).各实验组分别与相应的阴性对照组相比,结果与上述类似.结论复苏犬心肌线粒体Ca2+ATP酶活性及Ca2+含量的变化可能参与了复苏后心肌损伤的发生.     

兔心肌挫伤心功能障碍与心肌细胞内Ca2+和线粒体ATP酶的变化

目的：探讨心肌细胞内游离钙([Ca^2 ]i)和心肌ATP酶变化在心肌挫伤（MC）后心功能障碍发生机制中的意义。方法：随机选取兔36只，分为6组：正常对照、伤后2、4、8、12、24h，每组6只。经右颈总动脉插管至左心室测左室压力。采用BIM-II型生物撞击机致成MC模型。在上述时相点测定左室压力，并测定心肌细胞内[Ca^2 ]i和心肌匀浆组织及线粒体ATP酶活力。结果：心脏收缩/舒张功能受到损害，心肌细胞内[Ca^2 ]i升高，心肌匀浆组织及线粒体ATP酶下降，相关分析表明心功能障碍与心肌细胞内[Ca^2 ]i含量升高呈明显负相关，与ATP酶活性降低呈明显正相关。结论：MC后心脏功能下降，心肌细胞内[Ca^2 ]i含量升高和ATP酶活性下降可能是其原因之一。     

牛磺酸对钝性心脏损伤家兔心肌组织形态学的影响

目的 探讨牛磺酸对钝性心脏损伤家兔心肌组织形态学的影响。方法 将健康中国家兔24只随机分为3组：对照组、心肌挫伤组和牛磺酸治疗组。利用BIM-Ⅱ型水平式生物撞击机撞击兔胸前区制备钝性心脏损伤模型。3组动物分别于撞击24h后对各组兔心肌组织进行光镜和电镜观察。结果 家兔被撞击后其心肌组织发生明显肿胀、出血、肌纤维断裂，心肌组织超微结构明显损害；牛磺酸治疗组心肌组织损害程度明显轻于挫伤组。结论 牛磺酸能减轻心肌组织损害，对钝性损伤心肌组织具有一定的保护作用。     

黄芪注射液对兔钝性心肌损伤的影响

目的:探讨黄芪注射液(ASI)对兔钝性心肌损伤的保护作用。方法:健康中国家兔24只随机分为3组:对照组、实验组与ASI治疗组,其中实验组和ASI组利用BIMⅡ水平式生物撞击机撞击兔胸前区制备钝性心肌损伤模型。两组动物分别于撞击前5min,撞击后0.5,2,4,8及24h自股静脉采血检测cTnI、SOD、MDA。撞击后24h摘取心脏,制备超薄切片,透射电镜下观察其超微结构变化,对照组除不撞击胸部外,余处理均同实验组。结果:实验组及ASI组动物血浆中cTnI含量在撞击后224h均明显高于对照组(P<0.05),ASI治疗组升高幅度不如实验组,且较实验组相同时点有所下降(P<0.05);实验组SOD活性在撞击后0.5h即开始下降,伤后8h降低至最低点,伤后24h有所升高,但仍低于对照组及撞击前水平(P<0.01),ASI治疗组SOD活性与实验组同时点相比均有升高(P<0.05);实验组MDA含量自撞击后0.524h均高于对照组、撞击前水平(P<0.05),ASI治疗组MDA含量较实验组相应时点均有所下降(P<0.05);心肌超微结构观察治疗组损伤较轻。结论:黄芪注射液能减少家兔心肌损伤后血浆中cTnI及MDA水平,升高SOD的活性,对钝性心脏损伤具有一定的保护作用。     

银杏叶提取物对大鼠急性肾缺血损伤的实验研究

目的　观察银杏叶提取物对大鼠急性肾脏缺血再灌注损伤的保护作用及其机理。方法　SD大鼠随机分为假手术对照组、单纯缺血再灌注组、缺血再灌注 +银杏叶提取物处理组。测定肾组织丙二醛 (MDA)、超氧化物歧化酶(SOD)、Na+ K+ ATP酶、Ca2 + ATP酶变化并观察肾脏病理学改变。结果　与假手术对照组相比 ,单纯缺血再灌注组肾小管上皮细胞呈明显的缺血性改变 ,MDA含量增加 (P <0 0 1) ,SOD、Na+ K+ ATP酶、Ca2 + ATP酶活性降低 (P <0 0 1) ;缺血再灌注 +银杏叶提取物处理组与单纯缺血再灌注组相比 ,MDA含量明显降低 (P <0 0 5 ) ,SOD活性显著升高 (P <0 0 1) ,Na+ K+ ATP酶、Ca2 + ATP酶活性增高 (P <0 0 1、P <0 0 5 ) ,肾小管上皮细胞缺血性改变减轻。结论　银杏叶提取物通过抗氧化 ,对急性肾缺血再灌注损伤有保护作用     

三七总皂甙对兔心肌挫伤治疗作用的实验研究

心肌挫伤(myocardial contusion, MC)常引起程度不等的心脏并发症 [1],严重威胁伤员的生命.三七总皂甙(panax notoginseng saponins, PNS)具有保护心肌、抗休克等作用,目前已用于治疗缺血性心肌损伤[2]、冠心病心绞痛[3]等疾病.本实验旨在探讨PNS对家兔钝性心肌挫伤的影响.     

黄连素对L-甲状腺素诱发大鼠心肌肥厚的保护作用

目的　研究黄连素对 L-甲状腺素 (L- Thy)诱发大鼠发生心肌肥厚的保护作用。方法　 SD大鼠腹腔注射 (ip) L-甲状腺素 0 .5 mg/(kg· d)× 10 d,造成心肌肥厚模型 ,同时用黄连素 30 mg/(kg· d)× 10 d灌胃 ,观察大鼠的心肌肥厚指数 ,心肌细胞膜 Na+ - K+ - ATP酶和肌浆网 Ca2 + - ATP酶活力 ,心肌 NO含量 ,心肌钙调神经磷酸酶 (Ca N)活力的变化。结果　与正常对照组相比 ,心肌肥厚组心肌肥厚指数、Ca N活力明显升高 (P<0 .0 1) ,而心肌NO含量、Na+ - K+ - ATP酶活力和 Ca2 + - ATP酶活力均显著降低 (P<0 .0 1)。黄连素治疗组心肌肥厚指数 ,Ca N活力显著低于肥厚组 (P<0 .0 1) ,心肌 NO含量、Na+ - K+ - ATP酶活力和 Ca2 + - ATP酶活力则显著高于肥厚组 (P<0 .0 1)。结论　黄连素对 L-甲状腺素诱导的大鼠心肌肥厚具有保护作用。     

TNF-α的心肌负性肌力作用机制研究

目的 :探讨肿瘤坏死因子α(TNF-α)对心肌的负性肌力作用机制。方法 :采用离体大鼠乳头肌张力测定 ,细胞内双波长钙荧光检测和 ATP酶分析方法。结果 :1TNF-α抑制大鼠右心室乳头肌的收缩力 ,2 0 U/ ml和 2 0 0U/ ml TNF-α灌流 10 min后 ,乳头肌收缩力分别减小到对照的 91%和 76 % (P均 <0 .0 1) ;2 TNF-α处理后对心肌细胞钙瞬态变化幅度无明显影响 (0 .97± 0 .0 5 vs0 .95± 0 .0 7,P>0 .0 5 ) ;3TNF- α明显抑制肌浆网 Ca2 + - ATPase活性 ,平均抑制率达到 2 0 % ;4 TNF- α拮抗肌浆网 Ca2 + - ATPase对底物中不同水平的 Ca2 +和 ATP的正反应性 ;5TNF- α对肌膜 Ca2 + - ATP酶和 Na+ / K+ - ATP酶活性无明显抑制作用。结论 :TNF- α抑制心肌收缩力的负性肌力作用机制可能部分与心室肌浆网 Ca2 + - ATP酶活性下降有关 ,而与膜上的 Ca2 + - ATP酶和 Na+ / K+ - ATP酶活性无关。     

支气管哮喘发作期红细胞膜ATP酶SOD、XOD的变化及意义

目的　探讨支气管哮喘发作期红细胞膜 ATP的变化及其与 SOD、XOD的相关性。方法　对2 8例支气管发作患者和 5 0例正常健康人采用 Hanahan测定 Na+ - K+ - ATP酶 ;采用 Lathral测定 Ca2 + - ATP酶 ;采用 Pyragllal- ABT法测定 SOD;采用分光光度法测定 XOD。结果　患者 Na+ - K+ - ATP酶和 Ca2 + - ATP酶活性明显低于正常对照组 ( P <0 .0 1 ) ;患者 XOD明显高于正常对照组 ( P <0 .0 1 ) ,SOD明显低于正常对照组 ( P <0 .0 1 ) ;红细胞膜 Na+ - K+ - ATP酶和 Ca2 + - ATP酶活性与血 XOD呈高度负相关性 ( r=- 0 .871 ,P <0 .0 1 ,r=- 0 .685 ,P <0 .0 1 )。结论　支气管哮喘患者体内自由基生成增加 ,体内清除氧自由基的功能降低 ,从而引起细胞膜 ATP酶的损伤 ,干扰了细胞内外 Ca2 + ,Mg2 +的交换 ,这可能是哮喘发病的重要环节之一。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…