透明质酸在预防妇产科腹盆腔手术术后粘连中的作用

目的:探讨透明质酸在妇产科腹盆腔手术术后粘连预防中的临床效果。方法:选取我院2010年8月～2012年5月的120例妇产科腹盆腔手术患者为研究对象,随机分为对照组和观察组各60例,观察组在对照组的基础上加用透明质酸进行术后粘连预防,统计分析两组患者的术后粘连发生率。结果:观察组中初次手术和再次手术患者的粘连发生率及重度粘连发生率均低于对照组,差异有统计学意义(P<0.05)。结论:透明质酸在妇产科腹盆腔手术术后粘连预防中的临床效果明显,可显著降低粘连发生率。     

透明质酸钠凝胶用于剖宫产术中防粘连促愈合的应用体会

目的 评价透明质酸钠凝胶用于剖宫产术中预防粘连及促切口愈合的效果及安全性.方法将首次剖宫产手术的患者分为治疗组和观察组.治疗组患者于手术结束关腹前,将透明质酸钠凝胶10ml涂抹于子宫切口表面、输卵管卵巢周围、肠管及腹膜切口部位后按常规关腹.观察组患者未涂抹透明质酸钠凝胶.结果使用透明质酸钠的治疗组再次手术时发现盆腔粘连10例（0.09%）,腹腔内轻度粘连8例,中、重度粘连2例;无粘连25例（0.23%）.未使用透明质酸钠的观察组再次剖宫产手术时发现盆腔粘连21例（0.21%）,腹腔内轻度粘连13例,中、重度粘连8例;无粘连14例（0.14%）.结论透明质酸钠凝胶在剖宫产后预防盆腔粘连及促进切口愈合效果明显,特别是能有效减少分离后再粘连的发生.     

透明质酸钠预防宫腔镜粘连分离术后再粘连发生的效果评价

目的对行宫腔镜粘连分离术的患者给予透明质酸钠治疗,评价其对宫腔镜术后预防再粘连的效果。方法选取2015年1月至2017年1月本院收治的中重度宫腔粘连患者72例,将其随机分为研究组(n=36)与对照组(n=36),对照组宫腔放置宫内节育器+口服戊酸雌二醇孕激素人工周期治疗,研究组术后1~2月内重复宫腔镜检查+宫腔注入透明质酸钠+口服戊酸雌二醇孕激素人工周期治疗,术后对两组月经量改善、子宫内膜厚度及再粘连发生情况等进行比较。结果在月经改善率及子宫内膜厚度方面,研究组与对照组比较均显著提高(P0.05);在宫腔再粘连发生率方面,研究组与对照组相比显著降低(P0.05)。结论对宫腔粘连患者术后进行透明质酸钠综合治疗能够使月经明显改善,使子宫内膜恢复时间明显缩短,且可降低再粘连率,值得临床推广应用。     

透明质酸钠凝胶用于剖宫产术后预防盆腔及切口粘连的作用研究

目的通过B超粘连评价和体征评价,来评估透明质酸钠凝胶在剖宫产术中预防盆腔及切口粘连的作用。方法选择2013年1月至2015年1月本院收治的行剖宫产的孕妇400例,随机分为观察组200例和对照组200例,观察组术中使用医用透明质酸钠凝胶,对照组行常规手术。术后3个月随访,行B超检查和体征检查,进行粘连程度评估并记录。结果观察组B超检查结果为非致密粘连率3%,致密粘连率0.5%,体征检查阳性率为2.5%;对照组B超检查结果为非致密粘连率23%,致密粘连率15.5%,体征检查阳性率为为37%,P值均<0.05,两组差异均有统计学意义。结论透明质酸钠可以有效抑制剖宫产术后的粘连反应,减少粘连并发症的产生。     

医用透明质酸钠预防甲状腺术后粘连的探讨

目的观察医用透明质酸钠对于预防甲状腺术后粘连的作用。方法214例(实验组)甲状腺手术患者,于切除甲状腺病灶后,取医用透明质酸钠3～6ml均匀涂抹于甲状腺等创面上,并与同期未用医用透明质酸钠的182例(对照组)甲状腺手术患者作对比,评估术后粘连情况,进行数据分析。结果实验组:甲状腺术后粘连26例,对照组:甲状腺术后粘连41例。两组患者甲状腺手术后出现术后粘连情况比较,差异有显著意义(P<0.01)。结论医用透明质酸钠对预防甲状腺术后粘连有满意的功效。     

人工流产术后应用透明质酸钠宫腔注射联合去氧孕烯炔雌醇效果观察

目的观察人工流产术后应用透明质酸钠宫腔注射联合去氧孕烯炔雌醇预防宫腔粘连的效果。方法选取在郑州大学第二附属医院顺利完成人工流产手术的200例稽留流产患者,随机分为2组,每组100例。2组术后均口服抗生素预防感染。对照组术后实施宫腔注射透明质酸钠,观察组术后用透明质酸钠宫腔注射联合去氧孕烯炔雌醇。比较2组的疗效。结果观察组患者术后阴道出血持续时间、月经复潮时间及宫腔粘连、盆腔感染、周期性腹痛等并发症发生率均优于对照组。差异均有统计学意义(P0.05)。结论对稽留流产患者实施人工流产术后,应用透明质酸钠宫腔注射联合去氧孕烯炔雌醇,能有效降低并发症的发生率,促进子宫内膜修复。     

医用透明质酸钠HA在子宫肌瘤术后预防粘连效果观察

目的观察医用透明质酸钠（HA）在子宫肌瘤术后预防粘连的效果。方法对本院86例子宫肌瘤手术患者进行临床随机分组观察,对照组采用常规手术方案,观察组在对照组基础上使用医用透明质酸钠HA,观察两组患者术后宫腹腔粘连情况及相关并发症。结果观察组术后粘连率为4．65％,明显低于对照组的23．26％（P〈0.05）。此外,观察组各类并发症比例为4．65％,亦较对照组的13．95％为低（P〈0．05）。结论HA能够有效预防子宫肌瘤患者术后出现粘连,且能够降低术后并发症。     

妇科盆腔手术中应用透明质酸对粘连发生及腹腔液粘连相关指标的影响

目的分析妇科盆腔手术中应用透明质酸对粘连发生及腹腔液粘连相关指标的影响,为临床治疗提供参考依据。方法选取2015年6月至2017年6月医院收治的行妇科盆腔手术的患者60例,手术中应用透明质酸干预,将其作为研究组;另选取同期医院收治的行妇科盆腔手术的患者60例,手术中应用常规干预,将其作为对照组。对两组患者粘连发生率及手术前后腹腔液粘连相关指标变化情况进行观察比较。结果在盆腔粘连总发生率方面,研究组3.3%与对照组16.7%比较明显降低,差异具有统计学意义(P0.05);在腹腔液粘连相关指标(LN、PCⅢ、PⅢP及FN)水平方面,研究组手术后7、14、28天各指标水平均明显低于对照组术后7、14、28天各指标水平,差异均具有统计学意义(P0.05)。结论妇科盆腔手术中应用透明质酸能够有效控制粘连的发生,降低粘连发生率,且能够显著改善腹腔液粘连相关指标水平,具有较高的临床应用价值。     

两种方法对宫腔镜下IUA切除术后再次粘连的预防效果分析

目的比较研究医用自交联透明质酸钠凝胶联合Foley球囊导尿管和常规透明质酸钠腔内注入联合口服戊酸雌二醇的两种方法对宫腔黏连TCRA术后再发粘连的临床预防效果。方法选取2014年1月至2016年1月本院收治的宫腔粘连患者120例,所有患者均行TCRA治疗,术后治疗医师根据数字表法将120例患者评分为两组。其中实验组60例,TCRA术后行医用自交联透明质酸钠凝胶联合Foley球囊导尿管预防性治疗;对照组60例,TCRA术后行常规透明质酸钠腔内注入联合戊酸雌二醇口服预防治疗。于治疗后3个月所有患者月经干净3~7天后行宫腔镜复查并进行AFS评分,评估两组患者治疗后宫腔黏连预防及整体恢复情况。结果术后3个月行宫腔镜检查并进行AFS评分,结果显示,实验组AFS评分有效率明显优于对照组,差异具有统计学意义(χ~2=5.1603,P0.05);两组患者术后第3、4个月的AFS评分比较,实验组在宫腔黏连的累及范围、黏连情况、月经情况及总体分数均显著低于对照组,差异具有统计学意义(P0.05)。结论医用自交联透明质酸钠凝胶联合Foley球囊导尿管应用对宫腔黏连患者TCRA术后再发粘连的预防效果更佳,对宫腔内组织的无瘢痕愈合及月经恢复具有更好的促进作用,适合临床医师选择应用。     

TCRA术后Foley球囊+自交联透明质酸钠凝胶在宫腔粘连患者中的应用效果

目的探讨宫腔粘连患者宫腔镜下宫腔粘连分离术(transcervical resection of adhesions,TCRA)术后应用Foley球囊+自交联透明质酸钠凝胶预防术后再粘连的临床效果。方法选取我院在2015年12月至2017年11月收治的中重度宫腔粘连患者120例,按照随机数字表法分为观察组和对照组,每组各60例;观察组在TCRA术后应用Foley球囊+自交联透明质酸钠凝胶预防再粘连;对照组在TCRA术后应用Foley球囊+普通医用透明质酸钠凝胶预防再粘连。术后3个月观察两组患者临床疗效及AFS评分、粘连范围、粘连类型、月经状况及再粘连状况。结果治疗后观察组临床有效率为86.67%(52/60),对照组临床有效率为71.67%(43/60),比较具有统计学差异(χ2=4.093,P0.05);术后3个月宫腔镜检查两组患者AFS总分、粘连范围评分、粘连类型及月经状况评分较术前均明显下降(P0.05);观察组AFS总分、月经状况评分明显低于对照组,差异具有统计学意义(P0.05);两组患者术后粘连范围、粘连类型比较差异无统计学意义(P0.05);术后3个月宫腔镜检查,观察组术后再粘连发生率为1.92%(1/52),对照组术后再粘连发生率为16.28%(7/43),比较具有统计学差异(χ2=4.566,P0.05)。结论宫腔粘连患者TCRA术后应用Foley球囊+自交联透明质酸钠凝胶预防粘连效果显著,能够有效降低AFS评分及月经状况,值得临床推广。     

Reduction of postoperative adhesions secondary to strabismus surgery in rabbits.

The efficacy of tissue coating with sodium hyaluronate solution or oxidized regenerated cellulose (Interceed) sleeves in reducing the occurrence and severity of postoperative adhesions following strabismus surgery was tested in rabbits in vivo and compared with the efficacy in this regard of treatment with balanced salt solution. The use of Interceed sleeves significantly increased formation of postoperative adhesions, but coating with sodium hyaluronate solution prior to and during surgery significantly reduced such formation.     

The place of sodium hyaluronate in glaucoma surgery

The advantages of sodium hyaluronate (Healon) in filtering surgery have been mostly theoretical, with no study of sufficient numbers to justify its use. The authors used sodium hyaluronate in 119 consecutive trabeculectomies. These were compared to a control group of 122 cases. The long-term intraocular pressure and visual acuity results between the two groups were indistinguishable. The only definite benefit noted with the use of sodium hyaluronate was the lower incidence of hyphemas. No side-effects were seen. Its ability to maintain a deep anterior chamber and encourage hemostasis makes sodium hyaluronate useful in teaching goniotomies as well as performing trabeculectomies in congenital glaucoma. Sodium hyaluronate can also be used to evacuate hyphemas and dissect adhesions with minimal trauma, and may be healthier for the corneal endothelium than air in anterior chamber reformations.     

Prevention of adhesions by bioresorbable tissue barrier following laparoscopic intraabdominal mesh insertion

Intraabdominal adhesions represent a significant problem because of the morbidity associated with adhesive disease, including small bowel obstruction, difficulties in reoperative surgery, and possibly chronic pain. Coating solution of sodium hyaluronate (Sepracoat; Genzyme Production-Surgical Products, Cambridge, MA) was studied in New Zealand white rabbits to determine its potential role for prevention of postoperative adhesions following laparoscopic intraabdominal mesh insertion. A 2-cm polypropylene mesh was inserted laparoscopically to the left iliac fossa and fixed to anterior abdominal wall using a single prolen suture. Group 1 (n = 10) acted as the control group. Mesh was coated using 4% sodium hyaluronate in phosphate buffered saline (Sepracoat) in Group 2 (n = 10). Fourteen days later, all animals underwent diagnostic laparoscopy, and findings were recorded. All animals then were killed, the abdominal cavities were inspected, and adhesions were graded from 0 to 4. All meshes were removed and sent for histologic examination. The degrees of inflammation, fibrosis, and congestion were scored. No adhesions were seen on trocar sites on both groups. Eight of 10 animals in the control group and 5 of 10 animals in the study group had intraabdominal adhesions. The scoring of adhesions revealed that study group had only one (10%) significant adhesion, whereas the control group had eight (80%; < 0.001). Our study suggests that the Sepracoat reduces the incidence and severity of abdominal adhesions following laparoscopic mesh insertion and should be considered as a prophylactic agent, especially in those undergoing laparoscopic transabdominal mesh repair for hernia.     

奥曲肽联合透明质酸钠预防兔术后腹膜粘连的疗效评价

目的: 观察奥曲肽联合透明质酸钠预防兔术后腹膜粘连的效果。方法:建立兔术后腹膜粘连模型，然后分为4组：（1）术中不用药物处理设为模型对照组;（2）关腹前局部涂抹透明质酸钠设为透明质酸钠组;（3）关腹前腹腔内注射奥曲肽设为奥曲肽组;（4）关腹前局部涂抹透明质酸钠同时腹腔内注射奥曲肽设为联合组。术后14d剖腹观察，判定腹膜粘连程度等级。结果:4组粘连发生率比较无统计学意义(χ2＝3.51， P>0.05);联合组重度粘连发生率(8.3%)显著低于其它3组（分别为66.7%,33.3%,25.0%）(均P<0.01)。奥曲肽组和透明质酸钠组两组的粘连级别近似（P> 0.05）。结论:奥曲肽和透明质酸钠均可减轻实验性腹膜粘连的程度和重度腹膜粘连发生率，两者合用其作用更明显，表明两药合用具有降低粘连的协同作用。     

Sodium hyaluronate enhances colorectal tumour cell metastatic potential in vitro and in vivo

BACKGROUND: Sodium hyaluronate has been used intraperitoneally to prevent postoperative adhesions. However, the effect of sodium hyaluronate on tumour growth and metastasis in vitro and in vivo is still unknown. METHODS: Human colorectal tumour cell lines SW480, SW620 and SW707 were treated with sodium hyaluronate (10-500 microg/ml) and carboxymethylcellulose (0.125-1 per cent), and tumour cell proliferation and motility were determined in vitro. For the in vivo experiments male BD IX rats were randomized to a sodium hyaluronate group (n = 11; intraperitoneal administration of 0.5 x 10(6) DHD/K12 tumour cells and 5 ml 0.4 per cent sodium hyaluronate) or a phosphate-buffered saline group (n = 11; 0.5 x 10(6) DHD/K12 tumour cells and 5 ml phosphate-buffered saline intraperitoneally). Four weeks later the intraperitoneal tumour load was visualized directly. RESULTS: In vitro sodium hyaluronate increased tumour cell proliferation and motility significantly. Sodium hyaluronate-induced tumour cell motility appeared to be CD44 receptor dependent, whereas sodium hyaluronate-induced tumour cell proliferation was CD44 receptor independent. In vivo there was a significantly higher total tumour nodule count in the peritoneal cavity of the sodium hyaluronate-treated group compared with the control (P = 0.016). CONCLUSION: Sodium hyaluronate enhances tumour metastatic potential in vitro and in vivo, which suggests that use of sodium hyaluronate to prevent adhesions in colorectal cancer surgery may also potentiate intraperitoneal tumour growth. Presented to the Patey Prize Session of the Surgical Research Society and the annual scientific meeting of the Association of Surgeons of Great Britain and Ireland, Brighton, UK, 4-7 May 1999     

Effect of intraocular aspiration of sodium hyaluronate on postoperative intraocular pressure

A prospective, randomized, masked study was conducted to evaluate whether intraocular aspiration of sodium hyaluronate used in cataract surgery influenced postoperative intraocular pressure (IOP). Ninety-nine patients (105 eyes) underwent uncomplicated extracapsular extractions with posterior chamber intraocular lens implantation using 1% sodium hyaluronate (AMVISC). In 53 eyes, sodium hyaluronate was aspirated from the anterior chamber prior to wound closure. Sodium hyaluronate was left in the anterior chamber of 52 eyes. The IOP of 33 of the patients was measured 4 hours after surgery. No significant difference was found between the pressure in the eyes from which the sodium hyaluronate had been aspirated and the pressure in those from which it had not. The IOP of all the patients was measured on the first postoperative day. The mean 24 hours after surgery was 23.4 mm Hg in the aspirated eyes and 23.1 mm Hg in the not-aspirated group. Thirteen eyes in the aspirated group and 14 in the not-aspirated group had pressures above 30 mm Hg during the first 24 hours after surgery. There were no significant differences in visual outcome, patient discomfort, corneal clarity, anterior chamber inflammation, or subsequent IOPs during 3 months postoperative examination. Aspiration of sodium hyaluronate at the end of cataract surgery does not appear to significantly reduce either the incidence or the degree of postoperative pressure elevations.     

透明质酸钠在微创治疗输卵管阻塞性不孕中的应用观察

目的：比较透明质酸钠凝胶与松解液在宫腹腔镜联合COOK导丝治疗输卵管阻塞性不孕患者的临床疗效。方法选择2011年10月～2013年10月输卵管阻塞不孕患者55例,分成治疗组28例和对照组27例。治疗组使用医用透明质酸钠凝胶30mL,对照组使用松解液30mL。比较两组术后宫内妊娠率、异位妊娠率及卵管通畅率。结果术后3个月两组阻塞情况比较,治疗组阻塞率为0,对照组为3.7%,差异有统计学意义（P＜0.05）。术后1年内两组患者妊娠情况比较,治疗组宫内妊娠率60.7%、异位妊娠率7.1%,对照组宫内妊娠率33.3%、异位妊娠率29.6%,差异有统计学意义（P＜0.05）;两组患者输卵管通畅情况比较,治疗组通畅率75.0%、通而不畅率14.3%,对照组通畅率44.4%、通而不畅率40.7%,差异有统计学意义（P＜0.05）。结论在宫腹腔镜联合COOK导丝治疗的基础上使用透明质酸钠凝胶能够有效减少手术损伤,预防术后粘连,提高患者宫内妊娠率,降低异位妊娠率。     

Prevention of peritendinous adhesions following flexor tendon injury with seprafilm

Peritendinous adhesions are the most important complication of flexor tendon injury. In this study, Seprafilm was used for the prevention of peritendinous adhesions following flexor tendon repair. Seprafilm Bioresorbable Membrane (Genzyme Corporation, Cambridge, MA) contains sodium hyaluronate and carboxymethyl cellulose. Thirty New Zealand white male rabbits were divided equally into 3 groups. In all groups, the deep flexor tendon of the third finger of the left back foot was cut and repaired by Kessler-Tajima suture technique. In the first study group following tendon repair, Seprafilm was wrapped around the repaired tendon. In the second study group, sodium hyaluronate gel was injected to the operation field after tendon repair. In the control group, no external material was applied to the field. The study groups had better range of motion. Histopathologically, study groups had less adhesions compared with the control groups. As a result, it was concluded that in rabbit the peritendinous adhesions following flexor tendon repairs could be lowered with Seprafilm and hyaluronic acid.     

Exogenous hyaluronate as an adjunct in the prevention of adhesions after flexor tendon surgery: a controlled clinical trial.

Postoperative adhesions that restrict tendon gliding are a major cause of failure after tendon repair and grafting in zone II. Several experimental reports have claimed that exogenously administered sodium hyaluronate helps to prevent the formation of such adhesions. In a prospective double-blind, randomized, clinical study with open therapeutic control sodium hyaluronate or physiologic saline solution was injected into the tendon sheath after completion of tenorrhaphy or tendon grafting in 120 digits. Sodium hyaluronate had no statistically significant effect as evaluated on total active motion at follow-up.     

Effect of sodium hyaluronate on trabeculectomy filtration blebs

A prospective, randomized study was carried out to investigate the effect of 1% sodium hyaluronate on the formation and function of trabeculectomy filtration blebs. Using a standardized operation, a group of patients had sodium hyaluronate introduced below the scleral and conjunctival flaps. In a control group the operation was done without sodium hyaluronate. Analysis of anterior chamber depth, postoperative intraocular pressure and the size and appearance of the bleb was carried out. The results showed that the use of sodium hyaluronate gives a significantly deeper postoperative anterior chamber but also suggested that it results in a filtration bleb which tends to be thinner walled and loculated.