高效阴离子交换色谱-脉冲安培法测定b型流感嗜血杆菌结合疫苗的多糖含量

目的采用高效阴离子交换色谱-脉冲安培检测法(high performance anion exchange chromatography withpulsed amperometric detector,HPAEC-PAD)测定b型流感嗜血杆菌(haemophilus influenzae type b,Hib)结合疫苗的多聚磷酸核糖基核糖醇(polyribosylribitol phosphate,PRP)多糖含量。方法用终浓度为0.3 N的NaOH溶液将待测疫苗中的PRP多糖或分离得到的游离多糖在室温下振荡水解(16±8)h,采用CarboPac誖PA-10阴离子交换柱在NaOH/醋酸钠溶液梯度洗脱条件下分离水解产物,用脉冲安培检测器检测信号,根据样品的峰面积计算得到待测疫苗中的PRP多糖含量及游离多糖含量,样品均重复检测2次,计算2次检测结果的相对标准偏差(RSD)。同时采用《中国药典》三部(2010版)附录ⅧJ中的地衣酚法测定相同批次样品的PRP多糖含量及游离多糖含量,并对两种方法得到的结果进行比较。结果相同样品重复检测2次,结果间的差异非常小,RSD不超过4.6%。HPAEC-PAD法测得的总PRP结果与地衣酚法测得的结果接近,前者测得的S01～S03样品的PRP浓度值分别为后者的105%、104%和106%;HPAEC-PAD法测得的游离PRP结果与地衣酚法测得的结果差别较大,前者测得的S01～S03样品的游离PRP浓度值分别为后者的126%、109%和115%。结论 HPAEC-PAD法测定Hib结合疫苗的PRP多糖含量灵敏度高,重复性和分离效果好,样品不需要衍生处理,可同时测定多组分,可作为传统地衣酚法的替代方法用于Hib结合疫苗中多糖含量的测定。     

1株肠道高黏附性乳杆菌的分离鉴定

目的从人的肠道中分离具有高黏附性能的益生乳酸菌,为其应用于临床研究提供依据。方法采用改良的LAMVAB选择性培养基,取肠黏膜活检标本进行分级分离。对分离的乳酸菌进行生理、生化鉴定和16SrRNA序列测定,并对其耐酸、耐胆盐特性、黏附性能以及抑菌特性等进行分析。结果从人肠黏膜活检标本中分离出1株乳酸菌L2,该菌能在改良的LAMVAB选择性培养基上生长,并能抵抗pH2.5的酸性环境和0.3%的高胆盐环境;体外黏附试验显示,该菌株具有较好的黏附性能,对人肠上皮样细胞Caco-2和大鼠小肠上皮细胞IEC-6的黏附指数分别达(595±125.76)/100cells和(538±65.2)/100cells,且黏附具有Ca2+依赖性。对大肠杆菌、铜绿假单胞菌等具有较强的抑制作用,对益生菌鼠李糖乳杆菌LGG和嗜酸乳杆菌L05013-12无抑制作用。该菌株为植物乳杆菌(Lactobacillus plantarum)。结论植物乳杆菌L2具有较好的黏附性能,且耐酸、耐胆盐性能以及抑菌特性均较佳,具备作为益生菌使用的潜能。     

Evaluation of the bacteriological quality of ice cream sold at San Jose, Costa Rica

The presence of total and fecal coliforms, E. coli, Listeria sp and Salmonella sp. was evaluated in 65 samples of both commercial and homemade ice cream. 37.1% of homemade ice cream and 20% of commercial ice cream did not fulfill the international standard for total coliforms. At the same time 82.9% of home made samples and 56.7% of commercial ones presented fecal coliforms. E. coli was found in 51.4% of home made samples and 26.7% of commercial ones. Sixteen Listeria sp. isolates were obtained, 50% corresponded to Listeria monocytogenes and 50% to L. innocua. The overall presence of L. monocytogenes in ice cream samples was of 12.3% and it was isolated in all cases, from homemade ice cream samples. Salmonella was not isolated from the samples analyzed. Although the results obtained show an important improvement in the quality of ice cream, compared with a previous work done also in Costa Rica, further efforts shall be done, in order to offer safe products to consumers.     

Comparison of fatty acid content of imported peanuts

Variability in fatty acid compositions of peanuts imported from six different countries into the United States were studied to determine their effect on processing and storage conditions. The oil content ranged from 44.1 to 50.4%. Major fatty acids, palmitic acid (C16:0), oleic acid (C18:l), and linoleic acid (C18:2) ranged from 8.6 to 12.7, 35.9 to 61.1 and 21.7 to 44.2%, respectively. Oleic and lino-leic acids together comprised ca. 78.0–83.0% of the total fatty acids. Highly significant differences (P<.01) in fatty acid compositions were obtained between samples and between locations (countries of origin). Indicators of stability of the peanut samples as measured by the oleic/linoleic acid ratio (O/L) and iodine value (IV) of the extracted peanut oils showed variable but significant differences (P <.05) between locations. Generally, higher O/L ratios corresponding to lower IV indicate better stability and longer shelf-life of the samples.     

Biomolecular Characterization of Diazotrophs Isolated from the Tropical Soil in Malaysia

This study was conducted to evaluate selected biomolecular characteristics of rice root-associated diazotrophs isolated from the Tanjong Karang rice irrigation project area of Malaysia. Soil and rice plant samples were collected from seven soil series belonging to order Inceptisol (USDA soil taxonomy). A total of 38 diazotrophs were isolated using a nitrogen-free medium. The biochemical properties of the isolated bacteria, such as nitrogenase activity, indoleacetic acid (IAA) production and sugar utilization, were measured. According to a cluster analysis of Jaccard’s similarity coefficients, the genetic similarities among the isolated diazotrophs ranged from 10% to 100%. A dendogram constructed using the unweighted pair-group method with arithmetic mean (UPGMA) showed that the isolated diazotrophs clustered into 12 groups. The genomic DNA rep-PCR data were subjected to a principal component analysis, and the first four principal components (PC) accounted for 52.46% of the total variation among the 38 diazotrophs. The 10 diazotrophs that tested highly positive in the acetylene reduction assay (ARA) were identified as Bacillus spp. (9 diazotrophs) and Burkholderia sp. (Sb16) using the partial 16S rRNA gene sequence analysis. In the analysis of the biochemical characteristics, three principal components were accounted for approximately 85% of the total variation among the identified diazotrophs. The examination of root colonization using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) proved that two of the isolated diazotrophs (Sb16 and Sb26) were able to colonize the surface and interior of rice roots and fixed 22%–24% of the total tissue nitrogen from the atmosphere. In general, the tropical soils (Inceptisols) of the Tanjong Karang rice irrigation project area in Malaysia harbor a diverse group of diazotrophs that exhibit a large variation of biomolecular characteristics.     

以恶臭假单胞菌发酵制备D-氨基葡萄糖酸

从土样中分离到D-氨基葡萄糖酸高产菌株GNA5,综合Biolog细菌自动鉴定系统与16S rDNA序列分析结果,鉴定为恶臭假单胞菌(Pseudomonas putida). P. putida GNA5能耐受较高浓度底物D-氨基葡萄糖,可发酵积累D-氨基葡萄糖酸. 初步优化培养基为(g/L)：D-氨基葡萄糖盐酸盐30,葡萄糖5,尿素5, KH2PO4 2, MgSO4×7H2O 0.5, CaCO3 10, pH 7.0. 菌株GNA5在该培养基中发酵48 h可制备25.5 g/L的D-氨基葡萄糖酸,摩尔转化率达93.91%. 自行筛选的P. putida GNA5显示了底物转化能力强及产物分解能力弱的特点,具有进一步产业化开发的潜力.     

Determination of plasma tocopherols by gas liquid chromatography

A method is described for the separation and determination of plasma tocopherols by gas liquid chromatography (GLC). Proteins in 0.1 g samples of plasma were precipitated with ethanol containing a known amount of 5,7-dimethyltocol which served as an internal standard. Tocopherols were extracted into petroleum ether, purified by thin layer chromatography and analyzed as trimethylsilyl ethers by gas liquid chromatography (GLC) on 0.5% Apiezon L. Recoveries of α- and γ-tocopherols averaged 100% and 93%, respectively. The mean total tocopherol content of eight human plasma samples was 8.5 μg/g by GLC and 9.9 μg/g by a ferric chloride-α,α′-dipyridyl method. The α- and γ-tocopherol contents of 16 human plasma samples ranged from 4.0 to 12.3 μg/g and 0.6 to 2.1 μg/g, respectively.     

虎杖根提取物及分离组分对黄瓜白粉病的作用效果

在实验室内进行了虎杖（Polygonum cuspidatum Sieb.etZucc.）根提取物及其活性组分分离研究,并且对黄瓜白粉病进行了活性测定及温室小区试验。结果表明,无论提取率还是作用效果,乙醇较适合于做为虎杖根提取物的提取溶剂。虎杖根乙醇提取物和乙醚提取物对黄瓜白粉病的EC90值分别为537.85、35.38mg/L,而乙醚提取后残留物的活性很弱,说明乙醇提取物中具有杀菌作用的成分主要存在于乙醚提取物中。用pH梯度分离法对乙醚提取物进行分离,得到3个不同组分,其中以氢氧化钠分离得到的组分对黄瓜白粉病显示了极高的杀菌活性。在温室条件下虎杖乙醇提取物在800～1600mg/L下对黄瓜白粉病的防效相当或优于对照药剂三唑酮。     

A Newly Isolated Thermostable Lipase from Bacillus sp

A thermophilic lipolytic bacterium identified as Bacillus sp. L2 via 16S rDNA was previously isolated from a hot spring in Perak, Malaysia. Bacillus sp. L2 was confirmed to be in Group 5 of bacterial classification, a phylogenically and phenotypically coherent group of thermophilic bacilli displaying very high similarity among their 16S rRNA sequences (98.5–99.2%). Polymerase chain reaction (PCR) cloning of L2 lipase gene was conducted by using five different primers. Sequence analysis of the L2 lipase gene revealed an open reading frame (ORF) of 1251 bp that codes for 417 amino acids. The signal peptides consist of 28 amino acids. The mature protein is made of 388 amino acid residues. Recombinant lipase was successfully overexpressed with a 178-fold increase in activity compared to crude native L2 lipase. The recombinant L2 lipase (43.2 kDa) was purified to homogeneity in a single chromatography step. The purified lipase was found to be reactive at a temperature range of 55–80 °C and at a pH of 6–10. The L2 lipase had a melting temperature (Tm) of 59.04 °C when analyzed by circular dichroism (CD) spectroscopy studies. The optimum activity was found to be at 70 °C and pH 9. Lipase L2 was strongly inhibited by ethylenediaminetetraacetic acid (EDTA) (100%), whereas phenylmethylsulfonyl fluoride (PMSF), pepstatin-A, 2-mercaptoethanol and dithiothreitol (DTT) inhibited the enzyme by over 40%. The CD spectra of secondary structure analysis showed that the L2 lipase structure contained 38.6% α-helices, 2.2% ß-strands, 23.6% turns and 35.6% random conformations.     

Microbial conversion of palmitoleic acid to 9,12-hexadecadienoic acid (16:2ω4) by Trichoderma sp. AM076

Trichoderma sp. AM076, isolated from a freshwater sample, was found to accumulate 9,12-cis-hexadecadienoic acid (16:2ω4), when grown with palmitoleic acid (16: 1ω7). Methyl myristate was the best carbon source for the conversion of palmitoleic acid to 16:2ω4. The mycelial 16:2ω4 content reached 17.4 mg/g dry mycelia (443 mg/L) when the fungus was grown in a medium that contained 2.0% methyl myristate, 1.5% yeast extract, and 2.0% methyl palmitoleate, pH 6.0, for 5 d at 28°C with shaking. In both nonpolar and polar lipids from the mycelia, 16:2ω4 was detected as one of the major fatty acids when 16:1ω7 was added. It is probable that 16:1ω7 is converted to 16:2ω4 through the Δ12 desaturation reaction.     

Fatty acid and sterol compositions of malagasy tamarind kernel oils

The oil contents of six samples of Malagasy tamarind (Tamarindus indica L.) kernel were determined by hexane extraction (6.0–6.4%) and chloroform/methanol extraction (7.4-9.0%). The protein contents were very low (trace-0.1%). Investigation by gas liquid chromatography revealed 15 fatty acids, mainly palmitic (14–20%), stearic (6–7%), oleic (15–27%), linoleic (36–49%), arachidic (2–4%), behenic (3–5%) and lignoceric (3–8%) acids. Testing for the sterol fraction enabled seven sterols to be separated and quantitatively analyzed by gas liquid chromatography. The main sterols were β-sitosterol (66–72%), campesterol (16–19%) and stigmasterol (11–14%).     

一株脂肪酶产生菌及其脂肪酶催化性质的初步研究

从各种含油脂的土壤中筛选分离到一株高选择性拆分(R,S)-2-羟基-4-苯基丁酸乙酯[(R,S)-HPBE]的产脂肪酶菌株CF-12,经形态观察、生理生化试验和16S rDNA序列分析,鉴定该菌为沙雷氏菌属（Serratia sp.）。对菌株CF-12产脂肪酶的发酵条件进行了初步优化,确定最适产酶条件为：蔗糖5 g/L,酵母粉10 g/L,金属离子Mg²⁺ 0.75 mmol/L,发酵培养24 h,脂肪酶活性可达20.1 U/mL。该脂肪酶最适反应温度和pH值分别为40 ℃和7.0。利用冻干酶粉拆分 (R,S)-HPBE 15 h,(R)-HPBE产率达39.6%,ee=90.8%。     

Remote bitumen content estimation of Athabasca oil sand from hyperspectral infrared reflectance spectra using Gaussian singlets and derivative of Gaussian wavelets

Modeling of the total bitumen content, TBC, in Athabasca oil sands was undertaken on the basis of its hyperspectral reflectance spectra. Spectra (8 cm⁻¹ resolution) were obtained that covered both the short-wave infrared and thermal infrared (TIR: 3.00-30.00 μm). Two methods, Gaussian fitting and wavelet analyses, were investigated to identify useful bitumen features as well as the best removal of the baseline. We aim to obtain the best determination of the TBC for a suitable suite of test and validation oil sands samples. The Gaussian model relied explicitly on features at 2.282 and 2.532 μm though these were only two of 10 features simultaneously fit with a quadratic baseline to the range of 2.230-2.603 μm of the spectra. The wavelet model relied on bitumen features selected at 2.274, 2.396 and 3.725 μm that could be isolated from the baseline and noise. Both models yielded similar dispersion in their estimates of TBC (±∼1-2%) while the wavelet model proved to be more robust when applied to the validation and blind data suites. We also considered the effects of using the L2 optimization (classical least-squares) and L1 optimization (minimization of largest outlier) schemes for both models. Both schemes produced similar results for the model suite of samples for TBC but the L1 was superior when applied to the validation and blind data suites. The wavelet model using the L1 optimization appeared to be quite robust producing estimates of TBC (±∼1.0-1.7%).     

Revealing azo-dye decolorization of indigenous Aeromonas hydrophila from fountain spring in Northeast Taiwan

This study provides a first attempt to seek for indigenous strains with excellent decolorization capability for dye-laden wastewater treatment from an unpolluted and likely the most biodiverse region (Lanyang Plain) in Northeast Taiwan. For isolation, serial selections were conducted by using of the fungicide nystatin and model diazo-dye C.I. reactive red 141 (RR141). Thirteen bacterial strains (NIU 01-13) with the excellent capability of azo-dye decolorization were predominantly isolated from samples of a fountain spring near Chiao-Hsi in Lanyang Plain. According to profiles of protein expression and PCR-augmented 16S rRNA gene analyses for strain identification, >98% of gene sequences in isolated strains NIU 01 and NIU 12 were similar to Aeromonas hydrophila compared to available gene sequences in the NCBI BLAST gene bank. Phase-curve profiles indicated that azo-dye decolorization was found to be non-growth associated for isolated strains. Color removal efficiency of the culture was ca. 16-fold to that of Pseudomonas luteola, indicating a promising feasibility of isolated cultures to be used for practical treatments. This point apparently suggested that exploring a biodiverse indigenous microbiota with high performance of specific characteristics (e.g., color removal) from a unpolluted region (e.g., Northeast Taiwan) for practical applications should still be possible.     

Vitamins and minerals from sardine in tomato sauce, from the Mexican Pacific fish zones

Sardine is a sea food widely consumed in Mexico due to it's abundance and very low price. Its content in vitamins, minerals and chemical composition were evaluated in its canned presentation in tomato sauce. Samples proceeded from 3 fishery areas of the Mexican Pacific: (L1) Baja California Sur, (L2) Sonora and (L3) Sinaloa. The proximal chemical analysis was carried out by the AOAC methods; mineral content (Ca, Mg, Na, K, Fe, Zn, Cu, Cd, Cr, Pb) was determined by atomic absorption spectrophotometry and vitamins A, B1, B2 and niacin were quantified by HPLC. Results showed a high moisture content from 69 to 82%, ether extract ranged between 7.8% (L2) and 10.2% (L3) and crude protein content between 10.6% (L3) and 14.6% (L1). Vitamin A (IU/100 g) was similar for all samples: L1 (63-66); thiamin (mg/100 g) was high in L1 (0.13) and low in L3 (0.09); riboflavin was high in L1 (0.15) and showed values of 0.13 for both L2 and L3; niacin content was high in all samples: L1 (2.24), L2 (1.48) and L3 (1.75). Minerals were abundant (mg/100 g) in Na, K, and P; L1 showed a great variation for Na (363-636) compared with L2 and L3 (250); K was very variable among the samples: L1 (597), L2 (100) and L3 (57). Phosphorous and magnesium had small variations: P (229, 243 and 212) and Mg (28, 23 and 23) for L1, L2 and L3. Iron was more abundant in L1 (5.5) and L3 (4.3); Zn was higher in L2 (3.4) and similar in L1 (2.5) and L3 (2.2). Copper showed a great variation in L2 (0.21-0.48) but the averages were similar (0.37, 0.35, 0.33, for L1, L2 and L3 respectively). Finally, heavy metal content was lower than 0.002 mg/100 g. In conclusion, there are some variation in Ca, Na, K, thiamin and niacin content in canned sardine with tomato sauce, depending upon the fishing area.     

33株柯萨奇病毒A组16型分离株的全基因序列分析

目的对2008～2010年北京和青岛地区流行的33株柯萨奇病毒A组16型(Coxsackie virus A16,CA16)分离株的全基因序列进行分析。方法收集2008～2010年北京和青岛地区CA16感染患者咽拭子标本,采用Vero细胞对病毒进行分离,并经噬斑纯化。提取病毒RNA,采用RT-PCR法分段扩增CA16全长基因,经序列测定和拼接后,利用DNAStar和MEGA5.10软件分析全基因序列。结果经病毒分离和噬斑纯化,共获得33株CA16分离株;33个分离株之间的核苷酸序列同源性大于90.9%,与CA16国际标准株G10各区段的核苷酸序列同源性为72.7%～89.0%,氨基酸序列同源性为79.3%～100.0%;与近年中国分离的CA16 SZ/HK08-7株同源性较高,全基因组同源性大于91.5%,各区段核苷酸序列同源性均大于83.7%;在基于VP1序列的种系进化树中,BJWG16、BJWG17、BJWG20等23个分离株属于C1亚型,其余10个分离株属于C3亚型。结论 2008～2010年北京和青岛地区流行的33株CA16分离株均为C基因型。本研究对我国CA16分子流行病学、毒力位点的研究以及疫苗株的选择具有重要意义。     

Miscanthus sinensis fractionation by different reagents

Miscanthus sinensis L. was fractionated by different reagents (ethanol, soda and soda–ethanol) in order to obtain cellulose, hemicelluloses and lignin. Characterization of original M. sinensis fibres (66.6% holocellulose, 36.1% α-cellulose, and 15.5% lignin) was done and compared with other biomass species chemical composition (alternative raw materials, agriculture residues, coniferous and leafy plants). Obtained solid fractions were chemically characterized and compared with solid fractions from other biomass products (palm oil empty fruit bunches (EFB) and rice straw) generated by similar fractionation processes (soda and organosolv). Soda process produced the solid fraction with the highest content in α-cellulose and lowest content in lignin revealing a strong fractionation effect. On the contrary, soda–ethanol process was found to present low fractionation capability. Obtained cellulose samples were characterized by FTIR to complete the chemical structure analysis. Lignin samples isolated from the liquid fractions were submitted to FTIR, ¹H NMR, GPC, DSC and TGA in order to suggest suitable applications for the products based on their properties.     

蜡状芽孢杆菌菌株SMC的间甲酚降解特性及动力学

针对高浓度间甲酚废水难降解的问题,通过筛选获得一株高效降解间甲酚的菌株,并进一步探究其对间甲酚的降解特性和降解动力学.本文从某焦化厂活性污泥中经过驯化、筛选分离得到一株以间甲酚为唯一碳源的高效降解菌株,对其进行16S rDNA分子鉴定;通过测定培养液中间甲酚的剩余浓度,考察在菌株对间甲酚的最佳降解条件;并采用Haldane模型来模拟菌株降解间甲酚的动力学行为.将分离得到的菌株命名为SMC,初步鉴定为Bacillus cereus;在pH值为7.5、温度为30℃、摇床转速为150r/min的最佳培养条件下,菌株SMC在48h内对浓度1600mg/L的间甲酚降解率达97.81%,对间甲酚最大耐受能力为1900mg/L;经过Haldane模型拟合后的动力学参数为μ_max=0.01252h^-1,K_S=34.58mg/L,K_i=479.5mg/L(R²=0.932).菌株SMC对间甲酚有较强的降解能力,在高效处理含酚废水方面具有一定的应用前景.     

Chemical and fungal evaluation of graded sunflowerseed

Thirty-four samples of commercial oil-type sunflower-seed graded No. 1, 10 samples graded No. 2 and 33 samples graded Sample grade (SG) were used to study the relationship between percent heat damage, an important grading character, and percent FFA, percent color and UV absorption of extracted oil, percent germination and number and identities of fungi present. Seventy-two fungal species belonging to 28 genera were isolated. Thirty-seven fungal species in 17 genera were isolated from grade No. 1 seed; 68 species in 13 genera were isolated from grade No. 2 seed, and 68 species in 26 genera were isolated from SG seed. The genera most frequently isolated from grade No. 1 seed wereAlternaria (85.3%),Phoma (4.7%) andCladosporium (4.5%).Alternaria alternata was recovered from all No. 1 samples and comprised 75.6% of all isolates. The genera most frequently isolated from grade No. 2 seed wereAlternaria (74.5%),Eurotium (8.4%) andPhoma (7.7%). Fewer fungal species were isolated from grade No. 2 than from No. 1 seed, but a greater recovery of storage fungi was found for No. 2 seed (13.3%) than for No. 1 seed (2.5%).Alternaria (33.8%),Eurotium (33.1%) andMicroascus (8.3%) were the genera most frequently isolated from SG seed. Species of common storage fungi (Eurotium, Microascus, Penicillium andAspergillus) were recovered more frequently from SG than from Grades No. 1 and No. 2. In general, as the quality of the seed decreased from grade No. 1 to grade No. 2 to SG, there was an increase in percent heat damage, percent FFA, Lovibond color and UV absorption of extracted oil, and a decrease in percent germination. Analysis of variance of the quality characteristics data showed no significant differences between grade No. 1 and No. 2 seed except for UV 228 nm absorption. However, the quality characteristics of SG seed all differed significantly from those of grades No. 1 and No. 2 seed with the exception of percent seed yielding fungi. Correlation coefficients of the quality characteristics of SG showed a slight relationship between heat damage and percent FFA (r=0.63) and UV 228 nm absorbance (r=0.68). Although many of the SG seed were badly heat damaged, no statistical relationship was found between percent heat damage and percent seed yielding fungi or total isolates of storage fungi. The data in this study show that errors exist in grading decisions when seed are judged visually to be heat damaged.     

Sensory and chemical evaluation of stored oil-roasted,high oleic nonoil sunflower kernels

High oleic acid (HOA) and high linoleic acid (HLA) nonoil (confectionery) sunflower kernels were oil-roasted at 180 C and then stored at 27 C for up to 26 weeks (wk). At two- and/or four-wk intervals, samples were removed for chemical and sensory analyses. Fatty acid composition of the oils extracted from the roasted kernels were as follows: HOA — 16:0, 3.6%; 18:0, 3.3%; 18:1, 68.3%, and 18:2, 23.7%; regular HLA — 16:0, 5.0%; 18:0, 3.5%; 18:1, 29.0%, and 18:2, 62.6%. Hunter L and a values and hue angle for HOA and HLA kernels changed significantly (P<0.01) during 26 wk storage. Hunter a values for HLA did not change significantly but Hunter a values for HOA decreased significantly (P<0.01) during storage. Free fatty acids (FFA) of both the HLA and the HOA kernels increased significantly (P<0.01) beginning at 16 wk storage. In addition, the FFA from HLA kernels were significantly higher (P<0.01) than those from HOA kernels. Both types of stored, roasted kernels showed significant differences in sensory scores from the control samples (regular HLA type held at −35 C) beginning at four wk but rate of change throughout storage was similar for both kernel types. Sensory data were combined with objective parameters to analyze the multivariate data set by VARCLUS. Four clusters of attributes were extracted that explained 71.9% of the variation in the data. The data show there was a significant increase in off-flavor for both HLA and HOA kernels but no significant difference between the two types of kernels. Changes in the color of the sunflower kernels during storage evidently were not related to flavor quality.