一种用于检测橡胶树白粉菌的引物、方法及应用

正本发明提供了一种用于检测橡胶树白粉菌的引物、方法及应用。用于检测橡胶树白粉菌的引物包括引物对,引物对为如下引物对的任一种:1)SEQ ID NO.1所示的核苷酸序列及SEQ ID NO.2所示的核苷酸序列组成的引物对;2)SEQ ID NO.1所示核苷酸序列的互补核苷酸序列及SEQ ID NO.2所示核     

国内甲型副伤寒沙门菌疫苗株的16S rRNA基因序列分析

目的对我国4株用于或拟用于疫苗生产的甲型副伤寒沙门菌的16S rRNA基因进行序列分析,考察其保守性和差异。方法提取4株菌基因组DNA,以16S rDNA通用引物进行PCR扩增,并对产物进行克隆及测序。结果4株菌均可扩增出约1500bp的目的基因片段,测序结果表明,4株菌中16S rDNA核苷酸序列一致性达99.77%。结论选择保守性高的16S rDNA区段进行序列测定,以防止种子批的变异,便于对疫苗生产用种子进行质量控制。     

Gas-phase oxidation of NO at high pressure relevant to sour gas compression purification process for oxy-fuel combustion flue gas

The removal of NO from oxy-fuel combustion is typically incorporated in sour gas compression purification process. This process involves the oxidation of NO to NO_2 at a high pressure of 1–3 MPa, followed by absorption of NO_2 by water. In this pressure range, the NO conversion rates calculated using the existing kinetic constants are often higher than those obtained experimentally. This study aimed to achieve the regression of kinetic parameters of NO oxidation based on the existing experimental results and theoretical models.Based on three existing NO oxidation mechanisms, first, the expressions for NO conversion against residence time were derived. By minimizing the mean-square errors of NO conversion ratio, the optimum kinetic rate constants were obtained. Without considering the reverse reaction for NO oxidation, similar mean-square errors for NO conversion ratio were calculated. Considering the reverse reaction for NO oxidation based on the termolecular reaction mechanism, the minimum mean-square error for NO conversion ratio was obtained. Thus, the optimum NO oxidation rate in the pressure range 0.1–3 MPa can be expressed as follows:-d[NO]/dt=d[NO_2]/dt=0.0026[NO]~2[O_2]-0.0034[NO_2]~2 Detailed elementary reactions for N_2/NO/NO_2/O_2 system were established to simulate the NO oxidation rate. A sensitivity analysis showed that the critical elementary reaction is 2 NO + O_2? 2 NO_2. However, the simulated NO conversions at a high pressure of 10–30 bar are still higher than the experimental values and similar to those obtained from the models without considering the reverse reaction for NO oxidation.     

人GnRH及其转运肽基因合成及鉴定

目的人工合成人GnRH及转运肽(TRS)基因。方法根据已发表的人GnRH基因mRNA序列以及转运肽(9个左旋精氨酸)基因核苷酸序列,设计一对核苷酸,再用含7mol/L尿素的变性聚丙烯酰胺凝胶电泳纯化,然后以这对核苷酸3′末端短互补序列退火、补齐,合成长达90bp的目的序列GnRH/TRS。将其亚克隆到pMD18T载体上,构建重组质粒pYC1。酶切鉴定筛选出阳性克隆pYC1,并测序。结果GnRH/TRS序列由90个核苷酸组成,扩增产物与原设计序列同源性达100%。结论已成功合成人GnRH/TRS基因,为进一步研究和应用奠定基础。     

一种乙醛脱氢酶基因启动子及其应用

正本发明披露了一种乙醛脱氢酶基因启动子及其应用。本发明所提供的启动子为如下1)~3)中任意一种:1)序列表中序列1所示的DNA分子;2)在严格条件下与1)限定的序列杂交且具有启动子功能的DNA分子;3)与1)或2限定的DNA序列具有90%以上同源性,具有启动子功能的DNA分子;所述3)中的基因,与1)或2)限定的基因最好有95%以上的同源性。     

自养型生物过滤器硝化氧化一氧化氮

Carbon foam-a, kind of new engineering material as packing material was adopted in three biofilters with different pore dimensions and adapted autotrophic nitrite nitrobacteria to investigate the purification of nitric oxide (NO) in a gas stream. The biofilm was developed on the surface of carbon foams using nitrite as its only nitric source. The moisture in the filter was maintained by ultrasonic aerosol equipment which can minimize the thickness of the liquid film. The liquid phase nitrification test was conducted to determine the variability and the potential of performance among the three carbon foam biofilters. The investigation showed that during the NO2--N inlet concentration of 200g·L-1-min-1 to 800g·L-1-min-1, the 24PPC (pores per centimeter) carbon foam biofilter had the greatest potential, achieving the NO--N removal efficiency of 94% to 98%. The 8PPC and 18PPC carbon foam biofilters achieved the NO2--N removal efficiency of 15% to 21% and of 30% to 40%, respectively. The potential for this     

脉冲电晕结合Ca(OH)2吸收法脱硫脱硝

Removal of SO2 and NOx by pulsed corona combined with in situ alkali absorption was experimentally investigated.In the reactor,a plate-wire-plate combination is devised for generating pulsed corona and then alkaline absorbent slurries were introduced into the reactor by a continuous band conveying system to capture the gaseous reaction products.It was found that both SO2 and NO could be removed by corona combined with in situ alkali absorption.The removal of SO2 increased to 75%with the corona discharge,compared with 60%removal only with Ca(OH)2 absorption.About 40%removal of NO was reached by pulsed corona combined with in situ Ca(OH)2 absorption.It was found that SO2 and NO in the gas stream are oxidized to SO3 and NO2 by pulsed corona respectively,and then absorbed by the alkali in the reactor.The removals of SO2 as well as NO were higher with Ca(OH)2 as the absorbent,compared with using CaCO3 or ZnO.     

A study on simultaneous removal of NO and SO2 by using sodium persulfate aqueous scrubbing

Nitric oxide(NO) removal and sulfur dioxide(SO_2) removal by sodium persulfate(Na_2S_2O_8) were studied in a Bubble Column Reactor. The proposed reaction pathways of NO and SO_2 removal are discussed. The effects of temperatures(35–90 °C), Na_2S_2O_8(0.05–0.5 mol·L~(-1)), Fe SO4(0.5–5.0 m mol·L~(-1)) and H_2O_2(0.25 mol·L~(-1))on NO and SO_2 removal were investigated. The results indicated that increased persulfate concentration led to increase in NO removal at various temperatures. SO_2 was almost completely removed in the temperature range of 55–85 °C. Fe~(2+)accelerated persulfate activation and enhanced NO removal efficiency. At 0.2 mol·L~(-1) Na_2S_2O_8 and 0.5–1.0 mmol·L~(-1) Fe~(2+), NO removal of 93.5%–99% was obtained at 75–90 °C, SO_2 removal was higher than 99% at all temperatures. The addition of 0.25 mol·L~(-1) H_2O_2 into 0.2 mol·L~(-1) Na_2S_2O_8 solution promoted NO removal efficiency apparently until utterly decomposition of H2 O2, the SO_2 removal was as high as98.4% separately at 35 °C and 80 °C.     

与酶产生的过氧酸氧化染料的脱色方法、组合物和试剂盒

正种组合物和一种脱色染料的方法,包括使染料与一种含有水解酶酶,底物为水解酶酶和过氧化氢源其中过氧酸的水解酶酶对过氧化氢底物催化作用产生,其中染料接触一段时间的组合条件下,产生的过酸的量足以对至少一部分染料。这可能包括水解酶酶的氨基酸序列SEQ ID号1载,最好的水解酶酶是酶的S54V变异。酯基可以选自丙二醇二乙酸酯(丙二醇二醋酸酯)、乙二醇二乙酸酯(EGDA)、微量锡、乙酸乙酯、以及三丁酸甘油酯和过氧化氢可以在     

黄瓜细菌性萎蔫病菌分子诊断研究

从田间黄瓜细菌性萎蔫病植株分离获得病原菌,比对其近源种的16S rDNA序列,确定该病原菌为嗜维管束欧文氏菌(Erwinia tracheiphila),并设计出特异引物ET-P1/ET-P2.经过对该引物的PCR扩增条件优化,扩增出一条794 bp的黄瓜细菌性萎蔫病菌特异条带,检测灵敏度达100 fg/μL,可准确扩增出黄瓜发病植株和根围土壤中的DNA片段.