文昌鱼(Branchiostoma belcheri)离体细胞原代培养初步研究

分别以不同类型的培养液和盐度条件培养文昌鱼尾部、鳃、肝盲囊组织细胞. 观察显示经培养文昌鱼三类组织块在贴壁后24 h内细胞开始从组织块内迁移出来,并迅速增殖形成细胞晕,鳃组织细胞为圆球形状,尾部组织细胞为多边形上皮样细胞,肝盲囊组织细胞为圆球形状. 其中肝盲囊细胞和鳃组织细胞在含0.45% NaCl、15%小牛血清的Eagle's MEM培养粉中生长较其他培养条件好,而尾部组织细胞则在含0.9% NaCl、15%小牛血清的Eagle's MEM培养粉中生长较其他培养条件好. 这样的细胞能存活近40天,从而为文昌鱼体外细胞培养及建细胞系提供了依据.     

培养方法及胎牛血清浓度对人胎儿成纤维细胞体外生长的影响

采用单细胞培养法和组织块培养法从原代培养人胎儿成纤维细胞,并在不同体积分数胎牛血清(FBS)(0%、4%、8%、10%、12%)的条件下观察人胎儿成纤维细胞培养的状况并采用台盼蓝染色法进行活细胞计数.结果表明,早期由组织块培养法原代培养获取的细胞形态要比单细胞培养法原代培养获取的细胞稍好些,且在细胞传代后极少出现无法贴壁的死细胞.FBS对hFFs生长影响比较明显.在实际应用中,在体积分数8%FBS下,采用组织块培养法进行人胎儿成纤维细胞体外培养,效果良好.     

人胚肺细胞系(MU-L1)生物学特性研究

目的：对新建人胚肺细胞系(MU-L1)的生物学特性进行评价，以期建立可用于疫苗生产的人二倍体细胞株.方法：使用不同培养基及血清、不同血清浓度和传代比例培养MU-L1细胞，通过细胞形态、增殖速率和生长动力学等进行比较分析，筛选出较适宜的培养条件，用适宜的培养条件连续培养细胞至生命周期结束.传代过程中检查无菌、支原体和外源病毒因子，并进行染色体核型分析.结果：在常用培养基(MEM、DMEM、M199、DME/F12和PRMI1640)、胎牛血清和新生牛血清中MU-L1细胞均良好生长；按1∶4传代比例，使用含10%新生牛血清的MEM培养基培养，可培养至67代(世代),在生命周期的不同阶段经液氮冻存，复苏活率在91.56%～98.92%,生长良好.染色体核型为正常人二倍体核型，2n=46,无菌、支原体和外源病毒因子检查均为阴性.结论：MU-L1为正常人二倍体细胞系，具备作为病毒性疫苗基质进一步研究和生产应用的价值.     

胀果甘草愈伤组织培养及甘草酸含量分析

将胀果甘草的根、胚轴、子叶分别接种到含有不同激素组合的MS培养基上 ,在光照或黑暗下培养 .将根接种到液体培养基中培养 .结果发现不同的激素组合对愈伤组织生长和甘草酸质量分数的影响是不同的 .首先 ,随着 2 ,4D质量浓度上升 ,愈伤组织生长状况下降 ,甘草酸质量分数却增多 ,2 ,4D质量浓度为 4 .0时愈伤组织中甘草酸质量分数比生药中的增加 18.9% .加入BA或KT后 ,甘草酸含量明显增多 ,结果显示愈伤组织的生长与甘草酸的质量分数不成正相关 .其次 ,光照培养的愈伤组织比黑暗培养的生长快 ,但甘草酸含量低于黑暗培养 .第三 ,悬浮培养条件下 ,离体根培养物产生大量须根 ,根培养物中甘草酸质量分数明显高于其他培养物 ,比生药增多 4 3.3% ,反映出不同培养物产生甘草酸的能力不同 ,其中以离体根更适于甘草酸的合成 .     

鲤鱼吻端和尾鳍细胞的生长形态及增殖能力比较研究

采用组织块贴壁法,对鲤鱼的吻端和尾鳍进行细胞的离体培养,观察所获得细胞的形态,并总结每种细胞的形态学特点,同时绘制F5代细胞的生长曲线.研究结果表明,在原代细胞培养过程中,鲤鱼吻端细胞生长较快,以上皮样细胞为主,而鲤鱼尾鳍细胞生长较慢,以成纤维细胞居多.传代培养发现,通过控制胰蛋白酶消化的时间,尾鳍组织经2～3代筛选后可获得较纯的成纤维细胞.从F5代生长曲线看出,鲤鱼尾鳍细胞增殖能力略强于吻端细胞,因此鲤鱼尾鳍组织是较适宜的成纤维细胞体外培养材料.     

鳙鱼Aristichthys nobilis吻端细胞系BHS的建立及特性观察

以鳙鱼吻端组织为材料,建立了鳙鱼吻端细胞系,定命为 BHS。7个月期间细胞已传25代,选用培养基为 TC-199加20%或10%小牛血清。该细胞生长温度范围为14℃—35℃,最适温度28℃—30℃,形态主要为纤维样细胞,染色体数从55—82(2n=48)为异倍体细胞,克隆形成率较低为4.4%。     

斑节对虾组织的原代培养

选用Ｌ－１５,添加１０％～２０％ＦＢＳ,渗透压用ＮａＣｌ等调节,ｐＨ７．０,在２８℃下,原代培养斑节对虾的多种组织,发现淋巴器官,造血器官和血淋巴的组织细胞在较宽范围的培养基渗透压条件下能贴壁生长形成细胞单层．每升培养基添补７．５０ｇＮａＣｌ,０．３２ｇＫＣｌ,１．０２ｇＭｇＣｌ２６Ｈ２Ｏ,０．４９ｇＭｇＳＯ４７Ｈ２Ｏ,２．００ｇＣａＣｌ２２Ｈ２Ｏ和０．３０ｇ葡萄糖调节渗透压时,上述３种组织细胞生长最好     

剑麻组织培养诱导产蛋白酶研究

以剑麻株芽或幼叶为外植体,研究了不同培养基、不同培养温度和光照条件下,剑麻愈伤组织的诱导和生长特性．结果证明,Ｎ６培养基诱导剑麻愈伤组织启动最快;ＭＳ、ＬＳ培养基无论对愈伤组织的诱导还是继代培养都具有良好的效果;Ｂ５培养基不适于剑麻组织培养．剑麻愈伤组织诱导和生长最适宜的温度为（２５±２）℃、光照为１２ｈ．剑麻愈伤组织细胞可诱导产生蛋白酶．     

兔心房来源非心肌细胞的分离培养

建立一种兔心房组织来源的非心肌细胞体外培养模型,为心血管药理实验、病毒学实验及组织工程的研究提供实验平台.无菌条件下取兔心房壁组织,将组织分离成2 mm×1 mm小块,采用植块法以5行4列的形式种植于培养瓶中,加入M199或MEM培养基对兔心房组织进行原代培养.待长成单层时胰酶消化收集细胞,并进行传代培养和细胞冻存.该方法获得了大量活力良好的兔心房壁组织来源的非心肌细胞,倒置镜下观察细胞多呈短梭形,以及少量的三角形、柱形和卵圆形细胞,细胞核部位隆突,胞浆均匀清亮.经体外传代培养至16代,冷冻复苏以后,细胞仍生长良好.     

草鱼前体脂肪细胞原代、传代培养及鉴定

建立草鱼(Ctenopharyngodon idellus)前体脂肪细胞的培养体系,体外重现草鱼脂肪细胞的增殖分化过程.实验采用油红0染色法对脂肪细胞进行鉴定;采用倒置显微镜(NIKON)和CCD对细胞进行观察摄像;选用的健康草鱼体重为800～900 g;利用体外组织培养技术在温度为28℃,CO2浓度为5%,血清浓度为20%,pH值为7.0～7.2的条件下,以DMEM/F12培养基对来源于草鱼腹腔的脂肪组织进行原代培养;单层的原代培养细胞达到瓶底面积的70%～80%且汇合时.对细胞进行传代培养.研究发现在本实验条件下,培养48 h后组织块周围有梭形细胞迁出;3 d后细胞数量增多,多为梭形和多边形;培养8 d后大部分细胞融合;细胞内脂滴可被亲脂的油红0着色,证明为脂肪细胞.对融合后的细胞进行传代培养,可传至第4代;传代第3 d可形成致密单层;第8 d胞质内含有大量脂滴.研究初步确立了较为适宜的草鱼前体脂肪细胞离体培养体系.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.     

Tracking data management using topology of moving object database system for moving object constrained trajectory in LBS application

Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.