四川地区ODF1基因多态性与特发性弱精子症的相关性研究

本研究探讨四川地区外周致密纤维1(outer dense fiber1,ODF1)基因多态性与男性特发性弱精子症的相关性.选取106例特发性弱精子症男性不育患者为病例组和104例精液参数正常的男性为对照组,分析ODF1基因多态性在各组中分布的差异.结果表明:与对照组相比,ODF1基因中27-bp缺失变异体(c.656delACCCCTGCAGCCCCTGCAACCCGTGCA)的频率在特发性弱精子症男性不育组中显著升高(OR=1.783,95%CI=1.178-2.700,P=0.006),并利用MEGA比对分析后发现有10个氨基酸缺失(C218-P229delinsNPCSPCNPCS).通过PROVEAN与PMP软件分析,27-bp缺失变异体对蛋白质功能有潜在损害.因此四川地区ODF1基因中27-bp缺失变异体极有可能与弱精子症男性不育相关.     

云南大理白族和汉族弱精子症患者精子mtDNA 4977 bp缺失相关性研究

探讨云南大理白族和汉族弱精子症患者精子线粒体DNA(mtDNA) 4977 bp缺失的相关性。收集云南大理白族弱精子症患者137例,汉族弱精子症患者121例,正常对照组大理白族和白族精子活力正常人各120例,提取精液基因组DNA,采用聚合酶链式反应(PCR)技术进行精子mtDNA4977bp缺失的研究。结果显示,120例正常对照的大理白族精子活力正常人中有10例(8.33%)存在mtDNA4977 bp缺失;而137例大理白族弱精子症患者中有89例(64.96%)存在mtDNA 4977 bp缺失。两组4977 bp缺失频率比较有极显著性差异(P 0.01)。120例正常对照的汉族精子活力正常人中有7例(5.83%)存在mtDNA 4977 bp缺失;121例大理白族弱精子症患者中有85例(70.25%)存在mtDNA 4977 bp缺失。两组4977 bp缺失频率比较差异有极显著性(P 0.01)。同时发现大理白族弱精子症患者与汉族弱精子症患者mtDNA 4977 bp缺失频率无显著性差异(P 0.05)。大理白族和汉族人群mt DNA 4977 bp缺失与弱精子症的发病有明显的关联,提示mtDNA 4977 bp缺失在弱精子症的发生中可能起重要作用。     

自由基对人精子功能调控

人类精液中存在两种产生活性氧（ＲＯＳ）的来源－－精子和白细胞。这些ＲＯＳ在不同水平对精子结构和功能具有不同效应。结合本室成果,综述了ＲＯＳ及其清除剂对人精子存活率、运动性和获能等功能的调控及可能的作用机理,提示ＲＯＳ在男性生殖生理及病理过程中可能具有重要的生理病理作用,并为ＲＯＳ清除剂用于治疗男性不育症及抗男性生育提供理论和临床依据。     

自由基对人精子功能的调控

人类精液中存在着两种产生活性氧（ Ｒ Ｏ Ｓ）的来源——精子和白细胞．这些 Ｒ Ｏ Ｓ在不同水平对精子结构和功能具有不同效应．结合本室成果,综述了 Ｒ Ｏ Ｓ及其清除剂对人精子存活率、运动性和获能等功能的调控及可能的作用机理．提示 Ｒ Ｏ Ｓ在男性生殖生理及病理过程中可能具有重要的生理病理作用,并为 Ｒ Ｏ Ｓ清除剂用于治疗男性不育症及抗男性生育提供理论和临床依据     

中西药结合治疗感染性少、弱精子症临床分析

为探讨中西药结合治疗感染性少、弱精子症疗效,对73例存在不同部位生殖系统感染的少精子症或弱精子症引起的不育症患者应用相应抗生素治疗的同时均给予中药治疗,3个月为一个疗程,间隔3个月后复查精液常规,随访生育情况一年.结果:少精子症组随访中有9例妻子妊娠,妊娠率为33.3%.其中1例因剧烈活动妊娠2个月流产.弱精子症组随访中有17例妻子妊娠,妊娠率为37.0%,其中1例无明显原因妊娠2个月后流产.治疗前、后两组精液各项参数明显改善(P＜0.05).说明中、西药结合治疗感染性少、弱精子症起到标本兼治的疗效,少精子症、弱精子症妻子妊娠率均高于自然妊娠率,精液参数明显提高.     

RAD51基因135G/C单核苷酸多态性与男性不育的相关性研究

目的：研究RAD51基因135G／C单核苷酸多态性与男性不育的关系。方法：用PCR—RFLP技术，在255个正常生育男性和271例男性不育患者中，对RAD51基因135G／C单核苷酸多态位点的频率分布进行了分析。结果：在正常男性和男性不育患者组之间，RAD51基因135G／C的等位基因频率和基因型频率均无显著性差异（P值丝均大于0．05）。结论：RAD51基因135G／C单核苷酸多态性与男性不育的易感性不相关。     

一种新HDM2剪接变异体的cDNA克隆与序列分析

利用逆转录多聚酶链式反应(RT-PCR)分析癌基因HDM2在人宫颈癌Hela细胞中的表达.对克隆到的HDM2基因片段进行测序分析,从中筛选到一种新的HDM2剪接变异体.该剪接变异体阅读框由1 401 bp组成,预测编码466个氨基酸.与野生型HDM2相比,该变异体氨基酸序列的29-53位缺失,395住丝氨酸突变为苯丙氨酸,407位丝氨酸突变为半胱氨酸,其中缺失段29-53位于HDM2与p53相结合区域的上游部分.这可能影响HDM2与p53的相互作用,与p53的失活及癌细胞的转移相关.     

环境污染地区成年男性精子microRNA表达谱的建立

通过收集污染区男性精液标本30份和30份对照地区成年男性的精液标本,提取精子RNA,标记后与miRCU-RYTM LNA Array芯片杂交,分析环境污染与对照地区成年男性精子中miRNA的表达情况。结果表明环境污染与对照地区男性精子共182个miRNA存在表达差异,在环境污染地区73个miRNAs显著上调,109个m...     

ACE基因I／D多态性与郴州地区汉族2型糖尿病患者CIMT的关系

目的探讨血管紧张素转化酶（ angiotensin - converting enzyme, ACE）基因插入／缺失（insertion／deletion,L／D）多态性在郴州地区汉族2型糖尿病患者中的分布及其与糖尿病血管病变的关系。方法病程大于2年的郴州地区汉族2型糖尿病患者168例,依据是否合并高血压（hypertension,I-IT）、糖尿病肾病（diabeticnephropathy,DN）和糖尿病视网膜病变（diabeticrefinopathy,DR）等归类。采用PCR法确定其ACE基因多态性,高分辨彩超测量了其颈动脉内中膜厚度（carotid intima—media thickness,CIMT）,分析了I／D多态性与HT、DN、DR和CIMT的关系。用Logistic回归分析探讨了CIMT增厚的危险因素。结果ACE基因型在本组患者中分布为：DD型21．4％,ID型38．1％,Ⅱ型40．5％。3个基因型之间CIMT（P=0．001）、并发DN的比例（P=0．001）和合并HT的比例（P=0．005）有统计学差异。CIMT增厚的危险因素包括女性性别,DD基因型,并发DN和高龄。结论郴州地区汉族2型糖尿病患者ACE基因的D等位基因与HT、CIMT增厚及DN等血管病变正相关。CIMT增厚的危险因素包括女性,DD基因型,并发DN和高龄。     

非梗阻性无精子症患者细胞及分子遗传学效应研究

对103例非梗阻性无精子症患者进行常规外周血染色体G显带核型分析、AZF微缺失筛查、精浆生化指标和血清生殖激素定量检测.103例非梗阻性无精子症患者中,染色体核型异常16例,异常率15.53%,其中Klinefelter综合征8例,占异常总数的50.00%,其它性染色体异常5例,占异常总数的31.25%;AZF微缺失18例,缺失率17.48%,其中AZFc区缺失11例,占缺失总数的61.11%;染色体微缺失患者精浆锌浓度与染色体正常组患者比较,差异有统计学意义（P＜0.05）;染色体核型异常患者果糖浓度与染色体正常组患者比较,差异有统计学意义（P＜0.05）,尤其是Klinefelter综合征患者果糖浓度显著升高,差异有统计学意义（P＜0.01）;染色体核型异常患者FSH、LH均显著高于染色体正常组患者,差异有统计学意义（P＜0.05）,Klinefelter综合征患者T浓度则低于染色体正常组患者,差异有统计学意义（P＜0.05）;其他核型异常及Y染色体微缺失患者E2浓度显著高于染色体正常组患者,差异有统计学意义（P＜0.05）.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.