邻苯二甲酸二丁酯印迹吸附材料的合成及性能评价

在硅胶表面通过乙烯基三甲氧基硅烷接枝,以邻苯二甲酸二丁酯(DBP)为模板分子,丙烯酸为功能单体制备了DBP印迹聚合物。采用紫外光谱法(UV)研究单体与模板间的相互作用,扫描电子显微镜表征印迹聚合物的表面形貌,红外光谱分析聚合物的结构特征。用平衡吸附实验方法研究聚合物对DBP的结合性能,用Scatchard法分析了MIP-DBP的吸附解离常数(Kd)和吸附容量(Q)。结果表明,高亲和位点的解离常数Kd1=1.141 mmol/L,最大表观吸附量Qmax1=159.75μmol/g。低亲和位点的解离常数Kd2=3.697 mmol/L,最大表观吸附量Qmax2=299.66μmol/g。制备的印迹聚合物对DBP的结构类似物邻苯二甲酸二甲酯(DMP)和邻苯二甲酸二甲氧乙酯(DMOP)的分离因子(α)分别为2.04和1.57,表现出较好的选择性识别能力。     

苯胺印迹聚合物的制备及吸附性能研究

采用分子印迹技术,以苯胺为模板分子,甲基丙烯酸为功能单体,二甲基丙烯酸乙二醇酯为交联剂,制备了苯胺分子印迹聚合物。采用静态吸附法考察了影响该聚合物吸附苯胺的因素,并对等温吸附过程和吸附机理进行了判断。结果表明,该印迹聚合物对苯胺具有优良的吸附效果,在p H为6.0,底液苯胺的质量浓度为300 mg/L、吸附60min、投加量为2 g/L的条件下,该聚合物对苯胺的吸附量46.89 mg/g;该等温吸附过程符合Langmuir方程,饱和吸附量为52.52 mg/g,吸附动力学研究表明,该吸附过程符合准2级动力学方程,吸附速率常数为5.11 g/(g·min)。     

胆固醇分子印迹聚合微球的制备及其吸附性能

以胆固醇为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂,在甲苯-乙腈(9:1, j)混合溶剂中沉淀聚合制备了分子印迹聚合物微球. 采用平衡结合法和Scatchard模型评价了该聚合微球的结合性,考察了其吸附行为. 结果表明,胆固醇分子印迹聚合微球中形成两类不同的结合位点,得到高亲和性结合位点的离解常数和最大表观吸附量分别为0.86 mmol/L和80.4 mmol/g,低亲和性结合位点解离常数和最大表观吸附量分别为0.39 mmol/L和61.6 mmol/g. 此方法合成的分子印迹微球对胆固醇有较好的结合性能,可用于胆固醇的分析检测.     

溶胀聚合法制备微囊藻毒素分子印迹聚合物

以聚苯乙烯为种球,以铜绿微囊藻毒素(MC-LR)为模板分子,甲基丙烯酸(MAA)为功能单体,N′,N-亚甲基双丙烯酰胺为交联剂,通过溶胀聚合法得到分子印迹聚合物微球。采用电子显微镜、孔隙度分析、红外吸收等对其进行表征。并对制备铜绿微囊藻毒素分子印迹聚合物进行了优化条件实验(单体/模板/交联剂质量比为2660∶1∶3947;洗脱时间25 min)。通过Scatchard吸附模型分析,计算得离解常数Kd为3.7μmol/L,最大表观吸附量Qmax为124.7μg/g。     

等离子体引发制备亚甲基蓝印迹聚合物及其吸附性能研究

利用辉光放电等离子体代替传统化学引发剂,以亚甲基蓝为模板分子,丙烯酰胺为单体,N,N-亚甲基双丙烯酰胺作交联剂,采用沉淀聚合法制得分子印迹聚合物,用红外光谱和扫描电镜对其进行了表征。吸附实验表明印迹聚合物在20min达到吸附平衡,印迹聚合物对模板分子吸附量随浓度增加而增加,等离子体引发制备的印迹聚合物吸附量大于化学引发剂制得的印迹聚合物。扫描电镜结果表明等离子体引发制得的分子印迹聚合物颗粒粒径小、表面粗燥、孔穴多、比表面积大。采用等离子体引发制得的分子印迹聚合物对模板分子的吸附常数和最大吸附量分别为0.1528 L/mg和33.62 mg/g。     

利福平分子印迹聚合物的制备及其吸附行为

以利福平为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂,制备了利福平分子印迹聚合物. 采用平衡结合方法和Scatchard模型评价了该聚合物的结合特性,考察了其吸附行为. 结果表明,利福平分子印迹聚合物中形成了两类不同的结合位点,得到高亲和力结合位点的离解常数和最大表观结合量分别为31.5 mg/mL和23.34 mg/g,低亲和力结合位点的离解常数和最大表观结合量分别为9.22 mg/mL和12.86 mg/g. 实验结果显示,利福平分子印迹聚合物对利福平呈现出了高的选择吸附特性.     

沉淀聚合法制备吲哚乙酸印迹聚合物及其吸附性能研究

以吲哚乙酸(IAA)为模板分子、乙腈为致孔剂,采用沉淀聚合法制备了其分子印迹聚合物,通过静态吸附实验研究了致孔剂乙腈的用量及4-乙烯基吡啶(4-VP)和丙烯酰胺(AA)两种不同性质的功能单体(M)对印迹聚合物吸附性能的影响。结果表明,以4-VP为M,乙腈的加入量分别为20、25、37.5和50 m L时,制备的印迹聚合物P1~P4均具有良好的分散性,且聚合物P4对IAA具有最显著的印迹效应(印迹因子IF=2.92);而在同样条件下以AA为M的印迹聚合物的印迹效应较小(IF=1.73)。选择性实验表明,聚合物P4能够从吲哚羧酸同系物中选择性结合IAA。Scatchard分析表明,聚合物P4对IAA表现出两类结合位点,其高亲和力与低亲和力结合位点的平衡离解常数分别为Kd1=0.798 mmol/L和Kd2=7.76 mmol/L,对应的最大结合量分别为21.0和90.7μmol/g。     

邻苯二甲酸二丁酯分子印迹聚合物的制备及性能研究

以邻苯二甲酸二丁酯(DBP)为模板分子,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGD-MA)为交联剂,采用沉淀聚合法制备了分子印迹聚合物。通过静态平衡结合法和Scatchard分析法,研究了分子印迹聚合物的识别性能和结合能力。结果表明,该分子印迹聚合物对模板分子DBP具有较强的吸附特性和很好的选择性,其识别因子可达4.19。Scatchard分析表明,印迹聚合物微球存在2类不同的结合位点。高结合位点的平衡离解常数Kd1=0.078 mg/L,最大表观结合量Qmax1=53.03 mg/g;低结合位点的离解常数Kd2=0.023 mg/L,最大表观结合量Qmax2=33.95 mg/g。     

红霉素分子印迹聚合物纳米微球的制备及其吸附特性

以红霉素为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂,采用沉淀聚合法制备了粒径均一的红霉素纳米分子印迹聚合物微球,优化了分子印迹聚合物的合成条件,确定了模板分子与功能单体的最佳摩尔比为1:3,对其进行了表征. 结果表明,所制聚合物对红霉素的实际最大吸附量可达202.12 mg/g,吸附约200 min达到平衡,对红霉素具有良好的选择性吸附能力.     

结晶紫分子印迹微球的合成及其性能

以结晶紫(CV)为模板分子、α-甲基丙烯酸(MAA)为功能单体、二甲基丙烯酸乙二醇酯(EGDMA)为交联剂,采用沉淀聚合法合成结晶紫分子印迹微球。同时,考察合成过程中不同交联剂、模板分子与MAA的配比以及交联剂用量对微球性能的影响。结果表明:采用恒温水浴振荡法,以30 mL乙腈为溶剂,模板分子、功能单体和交联剂物质的量比为1∶4∶20,所制备的聚合物微球最大吸附量Q为39.1μmol/g,分离因子(α)和印迹效率因子(β)分别为3.76和3.18,对目标分子结晶紫具有良好的特异性识别和吸附能力。     

Application of Molecularly Imprinted Polymer for Extraction and Determination of Nalidixic Acid by High-Performance Liquid Chromatography

Nalidixic acid imprinted polymer has high application potential in clinical purposes. Molecularly imprinted polymers were prepared using nalidixic acid as the template molecule. The polymer was synthesized in a non-covalent approach with N,N-dimethylacrilamid and allyl glycidyl ether/iminodiacetic acid as the functional monomer. The effects of various parameters such as pH and adsorption time on adsorption capacity were considered. It was confirmed that the binding ability of the prepared molecularly imprinted polymer for nalidixic acid was essentially sufficient in the presence of the other compounds coexisting in human urine.     

沉淀聚合法制备烟酰胺分子印迹聚合物微球

以烟酰胺为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,乙腈为溶剂,采用沉淀聚合法制备了烟酰胺分子印迹聚合物,通过静态平衡吸附和色谱分析对印迹聚合物进行表征,结果表明,印迹聚合物对烟酰胺分子具有很好的吸附能力和特异识别性。     

壳聚糖表面胰蛋白酶分子印迹聚合物的制备及性能的研究

在壳聚糖表面通过希夫碱反应嫁接一层戊二醛,形成核-壳结构微球。然后在这个核-壳微球上,以胰蛋白酶为模板分子,3-氨基苯硼酸为功能单体,制备了胰蛋白酶分子印迹聚合物,通过静态吸附法,研究了聚合物的吸附性能。结果表明,印迹聚合物对模板分子有较高吸附容量和特异选择性。为从蛋白质混和溶液中分离富集胰蛋白酶提供了新的材料和方法。     

Molecular dynamics simulations of molecularly imprinted polymer approaches to the preparation of selective materials to remove norfloxacin

A novel molecularly imprinted polymer (MIP) designed by molecular dynamics (MD) simulations was successfully prepared with norfloxacin as a template molecule, methyl acrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a crosslinker. According to the theoretical prediction and experimental preparation methods, three kinds of molecular imprinting materials were designed and synthesized with MD simulations and molecular imprinting technology. The best ratio of the template to the functional monomer to the crosslinker was 1:8:40 in these studies. The experimental results illustrate that the MD simulations were credible in compounding the components of the MIPs. The structure of the prepared polymers were characterized with various methods. To analyze the adsorption performances, many kinds of static adsorption tests, including kinetic, isotherm, and selectivity tests, were used. The results indicate that the novel adsorbents conformed to the pseudo–second‐order kinetic equation and followed the Langmuir isotherm model. The adsorption amounts of MIP2 at a ratio of 1:8:40 were about 29.35 mg/g at 298 K_. The selective adsorption and reusable performance of norfloxacin were excellent. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 132, 42817.     

Adsorption selectivity of phenylalanine imprinted polymer prepared by the Wet Phase Inversion Method

A phenylalanine (Phe) imprinted polymer was prepared by the wet phase inversion method to investigate the optimum preparation condition which endows the polymer matrix with prominent adsorption selectivity. The addition of a large amount of porogen (dimethyl sulfoxide: DMSO) was needed to form macrovoids in the polymer matrix. For the high adsorption selectivity of the polymer matrix, a complex between functional monomers and a template molecule must be formed before the copolymerization of acrylomtnle (AN: crosslinker) and acrylic acid (AA: functional monomer). The formation of a phenyl group in the polymer matrix by the coupling reaction between D-Phe and AA seemed to cause the reverse adsorption selectivity of the polymer matrix. The adsorption selectivity of the Phe imprinted polymer was dependent on the pH of the racemate solution. The adsorption selectivity of the D-Phe imprinted polymer prepared by the post implanting method reached 11 at pH 2 and showed a reverse adsorption selectivity at pH 4 and 6.     

Synthesis of molecularly imprinted microspheres for recognition of trans‐aconitic acid

In the presence of a template molecule, trans‐aconitic acid and, using acetonitrile as solvent and dispersing medium, monodispersed microspheres with a diameter of 600–700 nm bearing molecularly imprinted binding sites were prepared by precipitation polymerization. It was found that the concentrations of template, monomer, and crosslinking agent as well as the chemical structure of the template greatly affect the polymer configuration. Microspheres are produced only when the concentration of the template molecule and the functional monomer are finely tuned. Comparison with the performance of a conventional imprinted polymer monolith showed that the imprinted microsphere had obvious advantages in specific binding to template molecule. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 94: 542–547, 2004     

分子印迹杂化材料的溶胶-凝胶法制备及选择性固相萃取牛奶中洛美沙星

以兽药洛美沙星为模板分子,3-氨基丙基三乙氧基硅烷为功能单体,甲基三甲氧基硅烷为交联剂,采用溶胶-凝胶分子印迹技术,以离子对及疏水作用方式,在水相体系中合成了具有分子识别作用的的新型有机-无机杂化分子印迹聚合物。静态吸附和Scatchard分析实验结果表明,此印迹材料对洛美沙星的结合存在两个结合位点,最大吸附容量分别为95.65与127.45μmol/g。以此印迹材料作为固相萃取剂,优化萃取条件,成功应用于牛奶中洛美沙星测定。     

Preparation of novel three-dimensionally ordered macroporous molecularly imprinted microspheres and its recognition for proteins

A novel protein molecularly imprinted microsphere with three-dimensionally ordered macroporous (3DOM) structure has been successfully prepared with a combination of colloidal crystal template method and molecular imprinting technique by using bovine serum albumin as template, acrylamide as functional monomer, and N,N′-methylenebisacrylamide as crosslinker, respectively. SEM shows that the polymeric microspheres have a highly ordered three-dimensional interconnected macroporous structure with typical face-centered cubic lattice. The isothermal and kinetic adsorption behaviors of the polymer were investigated. Compared with the traditional bulk polymer, the 3DOM imprinted microspheres show higher binding rate constant, adsorption capability and specificity to template molecules.     

不同功能单体制备的S-萘普生印迹聚合物材料的性能

以S-萘普生为模板,分别采用丙烯酰胺(AM)、N-乙烯基吡咯烷酮(NVP)和甲基丙烯酸(MAA)3种功能单体,合成了印迹聚合物P1、P2和P3。用UV和Chem 3D计算模拟,研究了功能单体与模板分子之间的相互作用,经PM3半经验方法计算得到AM、NVP、MAA与模板分子之间的相互作用能分别为-32.06、-21.01和-1.55kJ/mol。平衡结合实验测得当底物浓度为0.1 mmol/L时,P1、P2和P3的吸附量分别为0.360、0.305和0.150μmol;在浓度为0.1~4 mmol/L时,3种分子印迹聚合物对S-萘普生的吸附量大小关系为P1>P2>P3,这与计算出的功能单体与模板分子之间的相互作用能大小关系一致。