镰形棘豆总提取物的镇痛抗炎作用

目的研究藏药镰形棘豆总提取物的镇痛抗炎活性。方法使用小鼠扭体法和热板法观察镰形棘豆总提取物的镇痛效应,确定镇痛类型;采用小鼠耳廓肿胀法和大鼠棉球肉芽肿胀法观察镰形棘豆总提取物的抗炎效应。结果镰形棘豆总提取物经灌胃给药可明显减少由醋酸引起的小鼠扭体次数(P<0.05),但是不能明显提高小鼠热刺激的痛反应时间;可明显减轻二甲苯致小鼠耳廓肿胀程度和模型大鼠肉芽组织的增生。结论镰形棘豆具有良好的外周镇痛活性和抗炎活性。     

镰形棘豆经皮给药后抗炎镇痛活性有效部位的筛选

目的镰形棘豆经皮给药后,筛选其最佳抗炎镇痛有效组分。方法分离提取镰型棘豆的生物碱、总黄酮、挥发油部位,并进行组合,利用二甲苯诱导的小鼠耳肿胀法、热板法和醋酸扭体法筛选镰形棘豆不同有效部位镇痛抗炎活性的最佳组分。结果镰形棘豆总黄酮与挥发油有效部位组合物有更好的抗炎镇痛活性,其经皮给药后对小鼠耳肿胀的抑制率为90.22%;热板法实验中的镇痛率达87.85%;醋酸扭体法实验中镇痛率达73.23%,与阳性药效果相当。结论镰形棘豆总黄酮与挥发油组合物通过经皮给药方式有良好的抗炎镇痛活性。     

两头尖提取物的抗炎镇痛作用初探

目的研究两头尖提取物的抗炎镇痛作用。方法采用二甲苯致小鼠耳肿胀实验、醋酸致小鼠腹腔毛细血管通透性增高实验、新鲜蛋清致大鼠足跖肿胀实验、大鼠棉球肉芽增生实验考察两头尖提取物的抗炎作用,用小鼠扭体实验考察其镇痛作用。结果实验表明,两头尖提取物对各种炎症模型均有显著的抑制作用,并能显著减少醋酸致小鼠扭体反应次数。结论两头尖提取物有良好的抗炎镇痛作用。     

五鹤续断抗炎镇痛作用的实验研究

目的:探讨五鹤续断的抗炎镇痛作用。方法:抗炎实验采用二甲苯致小鼠耳廓肿胀等常规抗炎模型;镇痛实验采用热板法和醋酸扭体法。结果:五鹤续断提取物可明显抑制二甲苯致小鼠耳廊肿胀及蛋清引起的大鼠足跖肿胀,能显著抑制大鼠棉球肉芽肿的增重及醋酸致小鼠扭体反应,可明显延长热板引起小鼠疼痛反应的痛阈值。结论:富硒五鹤续断有明显的抗炎镇痛作用。     

头顶一棵珠抗炎镇痛作用的实验研究

目的:探讨头顶一棵珠的抗炎镇痛作用。方法:抗炎实验采用二甲苯致小鼠耳廓肿胀等常规抗炎模型;镇痛实验采用热板法和醋酸扭体法。结果:头顶一棵珠提取物可明显抑制二甲苯致小鼠耳廊肿胀及蛋清引起的大鼠足跖肿胀,能显著抑制大鼠棉球肉芽肿的增重及醋酸致小鼠扭体反应,可明显延长热板引起小鼠疼痛反应的痛阈值。结论:富硒头顶一棵珠有明显的抗炎镇痛作用。     

黄荆子不同溶剂提取物的抗炎镇痛作用

目的:观察黄荆子不同溶剂(氯仿、醋酸乙酯、水)提取部分的抗炎、镇痛作用。方法:采用二甲苯诱导小鼠耳郭肿胀和角叉菜胶诱导大鼠足肿胀建立炎症模型观察黄荆子不同溶剂提取物的抗炎作用;采用扭体法和热板法观察黄荆子不同溶剂提取物的镇痛作用。结果:氯仿提取部分(16,8 g.kg-1)对二甲苯诱导的小鼠耳肿胀抑制作用最为显著(P<0.01);氯仿提取部分(16 g.kg-1)能明显抑制角叉菜胶诱导的大鼠足肿胀(P<0.01)。对热和醋酸刺激引起的小鼠疼痛,水提物具有显著的镇痛作用(P<0.01),氯仿提取部分各剂量组镇痛作用不明显(P>0.05)。结论:黄荆子不同溶剂提取部位的抗炎镇痛作用有差异,氯仿提取物抗炎作用较强,水提取物的镇痛作用较强。     

苜蓿提取物抗炎镇痛作用的实验研究

目的研究苜蓿提取物抗炎镇痛的药理作用。方法应用二甲苯引起的小鼠耳肿胀,角叉菜胶致大鼠足趾肿胀、棉球所致大鼠肉芽组织增生、醋酸致小鼠扭体反应,及热水缩尾法疼痛模型观察了苜蓿提取物对炎症及疼痛反应的影响。结果苜蓿提取物可显著抑制二甲苯引起小鼠耳肿胀度,抑制率分别为78.72%、67.70%、46.03%;显著抑制角叉菜胶所致大鼠足肿胀,第6小时高、中、低剂量组的抑制率分别为47.86%、37.40%、34.84%;高、中剂量苜蓿提取物可明显抑制棉球致大鼠肉芽组织增生,其抑制率分别为32.26%、20.80%;对醋酸诱发的小鼠扭体反应有明显的抑制作用,其抑制率分别为65.99%、73.6%、55.84%;高、中剂量苜蓿提取物可延长热水引起疼痛的潜伏期,其延长率分别为51.55%、36.49%。结论苜蓿提取物具有明显的抗炎和镇痛作用。     

苦玄参提取物对小鼠的抗炎及镇痛作用

目的:研究苦玄参提取物对小鼠的抗炎及镇痛作用。方法:采用二甲苯诱导小鼠耳郭肿胀试验模型和腹腔毛细血管通透性试验观察抗炎作用;采用小鼠扭体试验观察镇痛作用。结果:苦玄参提取物能明显抑制二甲苯致小鼠耳肿胀和醋酸致小鼠腹腔毛细血管通透性增加;能减少冰醋酸刺激致痛小鼠的扭体次数。结论:苦玄参提取物对小鼠具有较好的抗炎及镇痛作用。     

黄荆叶不同溶剂提取物的抗炎镇痛作用研究

目的:观察黄荆叶不同溶剂(氯仿、乙酸乙酯、水)提取部分的抗炎、镇痛作用.方法:采用二甲苯诱导小鼠耳廓肿胀和角叉菜胶诱导大鼠足肿胀建立炎症模型观察黄荆叶不同溶剂提取物的抗炎作用;采用扭体法和热板法观察黄荆叶不同溶剂提取物的镇痛作用.结果:乙酸乙酯提取部分(16g·kg<'-1>、8g·kg<'-1>)对二甲苯诱导的小鼠耳肿胀抑制作用最为显著(P<0.01);氯仿提取部分(16g·kg<'-1>)能明显抑制角义菜胶诱导的大鼠足肿胀(P<0.01).对热刺激和醋酸刺激引起的小鼠疼痛,乙酸乙酯提取部分(16g.k<'-1>、8g.<'-1>)和水提物各剂量组具有显著的镇痛作用(P<0.01),氯仿提取部分各剂量组镇痛作用不明显(P>0.05).结论:黄荆叶不同溶剂提取部位的抗炎镇痛作用有差异,乙酸乙酯提取物具有明显的抗炎和镇痛作用.而氯仿提取物抗炎作用较强,水提取物的镇痛作用较强.     

三叶青提取物抗炎、镇痛及解热作用的实验研究

目的:探讨三叶青提取物(ERT)的抗炎、镇痛及解热作用,并验证其相关功效.方法:以小鼠腹腔毛细血管通透法、小鼠耳肿胀法及大鼠足肿胀法观察其抗炎作用;以小鼠扭体法、热板法观察其镇痛作用;以干酵母致热法及2,4-二硝基苯酚致热法观察其解热作用.结果:三叶青提取物能明显抑制小鼠腹腔毛细血管炎性渗出,抑制二甲苯所致小鼠耳廓肿胀及10%蛋清致大鼠足跖肿胀;减少醋酸致小鼠扭体次数,提高热板法小鼠痛阈值;并降低干酵母和2,4-二硝基苯酚致大鼠发热模型的体温.结论:三叶青提取物具有较好的抗炎、镇痛及解热作用,与其相关的中医临床功效及主治相符.     

白英提取物镇痛抗炎作用的实验研究

目的：初步探讨中药白英提取物的镇痛抗炎作用？方法：取白英的水提取物和乙醇提取物,应用醋酸致小鼠扭体法、小鼠热板法研究是镇痛作用;应用二甲苯致小鼠耳廓肿胀法和大鼠角叉莱胶足跖肿胀法研究其镇痛抗炎作用、结果：白英水提取物和乙醇提取物可减少醋酸致小鼠扭体次数,延长小鼠舔足时间;减轻二甲苯致小鼠耳廓肿胀程度,减小角叉莱胶致足跖肿胀程度。但水提取物作用强于乙醇提取物。结论：中药白英有一定的镇痛抗炎作用,其水提取物的镇痛抗炎作用比乙醇提取物效果明显。     

抗骨质增生巴布剂镇痛抗炎作用研究

目的：研究抗骨质增生巴布剂的镇痛、抗炎作用。方法：采用热板法、腹腔注射乙酸法造成小鼠疼痛模型；用二甲苯造成小鼠急性炎症模型，角叉菜胶、皮下包埋棉球法分别造成大鼠急、慢性炎症模型。将实验动物随机分为5组：空白对照组（等剂量的赋形剂），阳性对照南星镇痛膏组，高、中、低剂量抗骨质增生巴布剂组，分别给予相应药物，以考察抗骨质增生巴布剂的镇痛、抗炎效果。结果：抗骨质增生巴布剂能提高小鼠疼痛阈，延长疼痛潜伏期，降低扭体次数；显著降低小鼠毛细血管通透性，减少炎性介质的渗出；明显抑制由二甲苯引起的小鼠耳廓肿胀及角叉菜胶所致的大鼠炎症反应，降低白细胞数目；对大鼠肉芽组织增生有显著的抑制作用。结论：抗骨质增生巴布剂具有良好的镇痛、抗炎作用。     

Anti-inflammatory Properties of Entada abyssinica. Leaves

Abstract

The effects of the defatted methanol extract of Entada abyssinica. Steud. ex A. Rich leaves on some models of inflammation were investigated. The analgesic property of the plant extract was also tested on acetic acid–induced writhing, as well as formalin-induced paw licking, in mice. The antipyretic effect was evaluated using yeast-induced hyperpyrexia in mice. At doses of 50–200 mg/kg, the extract produced significant (p < 0.05) inhibition of leukocyte migration after intraperitoneal injection of carrageenan in rats. A topical anti-inflammatory effect was produced by 20 mg/ear of the extract, as demonstrated by inhibition of croton oil–induced ear edema in mice. The analgesic property of the plant extract was observed by inhibition of acetic acid–induced writhing and paw licking induced by formalin in mice. The extract, however, exhibited no antipyretic activity. This study further established the anti-inflammatory activity of E. abyssinica., in addition to its analgesic effect.     

Antinociceptive,antipyretic, and anti-inflammatory activities of <Emphasis Type="Italic">Putranjiva roxburghii</Emphasis> Wall. leaf extract in experimental animals

The effects of the ether extract from the leaves of Putranjiva roxburghii (P. roxburghii) Wall. were assessed on nociceptive responses in mice by using writhing, hot plate, and formalin tests and the antipyretic activity was determined in yeast-induced fever in rats. Anti-inflammatory activities were also investigated using carrageenin-induced paw edema in rats and croton oil-induced ear and anus edemas. The ether extract (100, 200, and 400 mg/kg, p.o.) of P. roxburghii dose-dependently produced analgesic activity in acetic acid-induced writhing in mice. The extract had no significant effect in the hot plate test in mice. At the dose of 400 mg/kg, the extract significantly suppressed the licking activity in the late phase of the formalin test in mice and decreased fever induced by yeast in rats. The extract exhibited moderate inhibitory activity of inflammation in carrageenin-induced paw edema in rats. The extract inhibited croton oil-induced ear edema in a dose-dependent manner (1.25, 2.5, and 5.0 mg/ear) in mice. The extract decreased anus edema induced by croton oil at the high dose of 800 mg/kg in rats. The results indicated that the ether extract of P. roxburghii leaves possesses analgesic, antipyretic, and anti-inflammatory activities.     

仙鹤草提取物镇痛抗炎试验的实验研究

目的:初步探讨中药仙鹤草提取物的镇痛抗炎作用.方法:取仙鹤草的水提取物和乙醇提取物,应用醋酸致小鼠扭体法、小鼠热板法、二甲苯致小鼠耳廓肿胀法和大鼠角叉菜胶足跖肿胀法研究其镇痛抗炎作用.结果:仙鹤草乙醇提取物和水提起物可减少醋酸致小鼠扭体次数,延长小鼠舔足时间,减轻二甲苯致小鼠耳廓肿胀程度,减小角叉菜胶致足跖肿胀程度;乙醇提取物作用强于水提取物.结论:中药仙鹤草乙醇提取物具有明显的镇痛抗炎作用.     

In vivo anti-inflammatory and analgesic activities of strictosamide from Nauclea officinalis

Context: Strictosamide is the main representative constituent of Nauclea officinalis Pierre ex Pitard (Rubiaceae), which has been used for a long time in China to treat diseases related to infection and inflammation, but its pharmacological activities are not well studied.

Objective: This work evaluates the anti-inflammatory and analgesic activities of strictosamide by in vivo experiments.

Materials and methods: The anti-inflammatory activity was assessed in mice by models of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema, acetic acid-elevated vascular permeability, and carboxymethylcellulose sodium (CMC–Na)-induced leukocyte migration. The analgesic activity was estimated in mice using acetic acid-induced writhing and hot-plate tests. Compound was injected to mice twice a day for 3?d at doses of 10, 20, and 40?mg/kg.

Results: At 20 and 40?mg/kg, strictosamide obviously decreased the TPA-induced mice ear edema (24.7 and 28.1% inhibition, respectively), and significantly inhibited acetic acid-stimulated peritoneal vascular permeability in mice (23.3 and 33.4% inhibition, respectively). It also significantly decreased the leukocytes in the mice peritoneal cavity induced by CMC–Na at all the tested doses (46.0, 49.1, and 58.7% inhibition, respectively). To acetic acid-induced writhing test in mice, strictosamide markedly prolonged the pain latency at 20 and 40?mg/kg and decreased the writhing counts at 40?mg/kg (49.7% inhibition). However, it did not obviously improve the pain threshold of mice in hot-plate test.

Discussion and conclusion: Strictosamide may have important effects on inflammation and inflammatory pain. The results provide scientific support for the role of strictosamide in the use of N. officinalis to treat inflammatory diseases.     

苯甲酰青藤碱的镇痛抗炎作用

目的:考察青藤碱衍生物苯甲酰青藤碱的镇痛、抗炎作用.方法:用小鼠扭体法、小鼠热板法、小鼠耳肿胀法、大鼠足跖肿胀法等试验观察.结果:苯甲酰青藤碱可抑制冰醋酸致小鼠疼痛的作用,提高小鼠热板痛阈值,抑制由二甲苯引起的小鼠耳肿胀,对角叉菜所致的足肿胀有抑制作用.结论:苯甲酰青藤碱具有镇痛、抗炎作用,且呈量效关系.     

Anti-inflammatory activity ofElsholtzia splendens

Elsholtzia splendens Nakai has been used in North-East Asia as an ingredient of folk medicines for treating cough, headache and inflammation. The present investigation was carried out to establish its in vivo anti-inflammatory activity using several animal models of inflammation and pain. The 75% ethanol extract of the aerial part of E. splendens significantly inhibited mouse croton oil-induced, as well as arachidonic acid-induced, ear edema by oral administration (44.6% inhibition of croton oil-induced edema at 400 mg/kg). This plant material also showed significant inhibitory activity against the mouse ear edema induced by multiple treatment of phorbol ester for 3 days, which is an animal model of subchronic inflammation. In addition, E. splendens exhibited significant analgesic activity against mouse acetic acid-induced writhing (50% inhibition at 400 mg/kg), while indomethacin (5 mg/kg) demonstrated 95% inhibition. E. splendens (5-100 microg/mL) significantly inhibited PGE2 production by pre-induced cyclooxygenase-2 of lipopolysaccharide-treated RAW 264.7 cells, suggesting that cyclooxygenase-2 inhibition might be one of the cellular mechanisms of anti-inflammation.