17β-雌二醇对去卵巢豚鼠结肠平滑肌的作用

目的:观察17β-雌二醇对去卵巢豚鼠结肠平滑肌的作用.方法:将豚鼠分为A组(去卵巢7 d)、B组(去卵巢+雌二醇3 d)、C组(去卵巢+雌二醇7 d).利用张力换能器记录豚鼠离体结肠平滑肌肌条对胆囊收缩素(cholecystokinin,CCK)的反应.结果:加入CCK后,三组平滑肌肌条收缩幅度均增加.增加幅度分别为0.096 g±0.015 g、0.134 g±0.026 g、0.179 g±0.027 g.B、C两组增加幅度较A组更为明显(P＜0.05),差别有统计学意义.结论:雌二醇能增强结肠平滑肌对CCK的敏感性.     

四种胃肠激素在糖尿病胃动力障碍中的作用

目的通过对糖尿病大鼠模型离体胃窦平滑肌自发性收缩运动的研究,探讨血浆和胃窦组织中生长抑素(SS),血管活性肠肽(VIP),胃动素(MTL),P物质(SP)等在糖尿病胃动力障碍中的作用。方法建立糖尿病大鼠模型,制备实验组和对照组大鼠离体胃窦环行肌及纵行肌肌条,应用张力换能器测定其静息张力、平均振幅、收缩频率等运动指标;用放免法同批测定两组大鼠血浆和胃窦组织中SS,VIP,MTL和SP含量。结果①糖尿病组胃窦肌条自发收缩运动的多项指标均较对照组明显降低(P<0.01);②糖尿病组血浆中SS、VIP、MTL增加,SP降低;胃窦组织中SS增加,MTL和SP降低,VIP无变化;③血浆和胃组织SS与胃运动指标呈负相关;血浆VIP与MTL与胃运动指标呈负相关;血浆和胃组织SP和胃运动指标无明显相关性。结论①糖尿病多伴有胃运动功能障碍;②血浆和胃组织中胃肠激素含量变化在糖尿病胃动力障碍的发病中有一定作用。     

促结肠动力中药筛选及其机制

目的:探讨中药生白术、小茴香、蒲公英、莱菔子、大腹皮、莪术的促结肠动力作用,以筛选出具有开发价值的促结肠动力药物,并探讨其初步机制.方法:制备豚鼠结肠纵肌,以9g/L氯化钠溶液和乙酰胆碱为对照,观察6味中药对离体结肠肌条收缩活性的效应.实验结果以振幅(g)、时间(s)和曲线下面积(g·s)表示.分析对象为加药后5min内出现的最大波形.结果:该6味中药对肌条均有不同程度的兴奋效应,中药组(依次为大腹皮、莱菔子、蒲公英、小茴香、莪术、生白术)与生理盐水组相比,振幅峰值(0.97±0.40,0.95±0.26,0.87±0.49,0.85±0.26,0.78±0.39,0.60±0.25vs0.03±0.04,P<0.01)和曲线下面积(1.40±1.00,0.75±0.79,0.62±0.54,0.64±0.59,0.40±0.37,0.29±0.15vs0.11±0.06,P<0.05或P<0.01)均有显著性差异,以大腹皮作用最强,时间长达175±238.07s.在阿托品和维拉帕米分别预孵育情况下,加入各中药后与单独中药组的肌条收缩幅度增强的幅度明显减弱,两组间差异有显著性(中药 拮抗剂组vs中药组,P<0.05).结论:该6味中药对结肠平滑肌均有不同程度的兴奋效应,引起的收缩效应可被M受体阻断剂阿托品部分阻断,通过细胞外Ca2 内流介导.     

P物质NK1受体对大鼠结肠动力的调节作用

[目的]探讨P物质(substance P,SP)不同受体对大鼠结肠动力的调节作用。[方法]取大鼠近端结肠制备纵行肌(LMS)及环形肌(CMS)肌条,用张力换能器及多通道生理信号采集处理系统观察SP及其受体拮抗剂对SP诱导的LMS和CMS收缩活动的影响。[结果]SP显著促进大鼠结肠LMS和CMS自发性收缩活动[LMS:正常(0.85±0.06)g∶SP(1.02±0.05)g;CMS:正常(0.22±0.03)g∶SP(0.81±0.07)g,P0.01];CMS孵育河豚毒素TTX后加入SP收缩幅度显著增加[正常(1.15±0.11)g∶SP(2.25±0.18)g,P0.05];NK1受体特异性阻断剂L-703孵育肌条后加入SP,平滑肌收缩无显著增强,NK2受体特异性阻断剂GR159孵育后,SP能显著诱导平滑肌收缩增强(P0.05)。[结论]NK1和NK2受体均参与SP对结肠动力的调节,且对CMS的调节过程中,NK1受体发挥更重要的作用,SP对LMS的调节可能主要通过肠神经系统。     

Nesfatin-1对肥胖大鼠胃排空及胃平滑肌条收缩性的影响

目的:观察Nesfatin-1对正常及单纯性肥胖大鼠胃排空及离体胃平滑肌条收缩活性的影响.方法:高脂饲料喂养♂大鼠6wk,制作单纯性肥胖大鼠模型;正常及肥胖大鼠实验组中枢注入不同浓度Nesfatin-1(0.5μmol/L、5μmol/L、50μmol/L)后测胃排空率;生理记录仪记录大鼠胃底、胃体平滑肌条自发收缩及不同浓度Nesfatin-1(0.026μmol/L、0.26μmol/L、2.6μmol/L)作用下对乙酰胆碱(Ach)诱导的肌条收缩的影响.结果:Nesfatin-1可抑制正常及肥胖大鼠胃平滑肌条收缩,低、中、高浓度组与生理盐水对照组相比差异均有统计学意义(q=3.93-15.72,P<0.05-0.01).随Nesfatin-1浓度的增高,其抑制作用呈明显剂量依赖关系(q=3.45-5.69,P<0.05-0.01).低浓度Nesfatin-1抑制正常大鼠、肥胖大鼠胃底平滑肌条收缩作用无显著性差异(P>0.05),中、高浓度对正常大鼠胃底平滑肌条抑制作用强于肥胖大鼠(t=2.14,P<0.05;t=2.63,P<0.05).低、中浓度Nesfatin-1抑制正常大鼠、肥胖大鼠离体胃体平滑肌条收缩作用无显著性差异(P>0.05),但高浓度抑制正常大鼠离体胃体平滑肌条收缩作用显著强于肥胖大鼠(t=2.53,P<0.05).结论:Nesfatin-1可抑制正常及肥胖大鼠胃排空,胃排空率随Nesfatin-1浓度增加而降低.Nesfatin-1可抑制乙酰胆碱诱导的单纯性肥胖大鼠离体胃平滑肌条的收缩活动,其抑制作用随Nesfatin-1浓度增高而增强;相同Nesfatin-1浓度抑制正常大鼠离体平滑肌条收缩作用强于肥胖大鼠.     

PACAP结构活性相关性的研究

目的运用合成的 VIP/PACAP 相关肽,在离体豚鼠胆囊探讨 PACAP 的结构与生物活性的相关性.方法用多品种同时氨基酸固相合成仪 Fmoc 法高效合成 VIP,PACAP 及其相关肽.用改良水平浴槽法测定比较各多肽对离体豚鼠胆囊的生物效应.结果 VIP 引起豚鼠胆囊平滑肌的舒张;与此相反,PACAP27则呈收缩效应,改变第4、第5和第24-26位氨基酸残基,对其生物效应没有明显影响.尽管[Leu~(13)]-PACAP27,是一个在α螺旋区域的变构体,但其收缩胆囊的活性并没有明显降低(P>0.05),而且较[Gly~8]-,[DAsp~8]-PACAP27和第21位的3个置换物更强.Des-[His~1]和[Ala~6]-PACAP27在10~(-7)mol/L 的浓度时未见生物活性,[Gly~8]-,[DAsp~8]-,[Phe~(21)]-和[Pro~(21)]-PACAP27在该浓度时收缩效应仅相当于 PACAP27的25%(P<0.05).结论在对豚鼠胆囊平滑肌的生物活性方面,PACAP27的 N 端(第1-8位)氨其酸残基较其他区域更为重要.     

白细胞介素6对豚鼠离体近端结肠平滑肌收缩的影响

目的:研究白细胞介素6(IL-6)对豚鼠近端结肠平滑肌的影响及其机制.方法:观察IL-6对结肠收缩的影响;用河豚毒素(TTX)阻断肠神经后观察不同浓度IL-6对结肠平滑肌收缩的影响;损伤Cajal间质细胞(ICC)后观察IL-6对结肠平滑肌收缩的影响.结果:在带有ICC的近端结肠纵行肌加入IL-6后,结肠平滑肌的收缩振幅增加和频率加快,呈浓度依赖性:加入TTX后,收缩的幅度和频率,同拮抗前相比分别降低和减慢(0.206±0.027 g vs 0.300±0.039 g;9.770±1.711 s vs 8.483±1.113 s:P<0.01,P<0.05):TTX阻断后加入IL-6(80μg/L),振幅增加和频率加快(P<0.01,P<0.05):破坏结肠ICC,收缩幅度和频率分别与损伤ICC后加入IL-6无显著性差异(80μg/L),而与正常结肠收缩振幅和频率有显著性差异(P<0.01).结论:IL-6对豚鼠近端结肠平滑肌的收缩活动有兴奋作用,其兴奋效应主要是通过肠神经元介导.ICC是IL-6对平滑肌兴奋途径的一个不可缺少的中间环节.     

钙离子在CNP抑制Ach增强胃窦环行平滑肌收缩效应中的作用

目的 探讨细胞内外钙离子在C型钠尿肽(CNP)对乙酰胆碱(Ach)引起的大鼠离体胃窦环行平滑肌收缩效应中的作用.方法 利用浴槽孵育离体大鼠胃窦环行肌肌条,用离体平滑肌张力记录装置记录平滑肌肌条收缩活动,观察钙离子对Ach引起的大鼠离体胃窦环行平滑肌收缩效应的影响.结果 CNP对Ach引起的胃平滑肌自发性收缩增强效应有明显的抑制作用,在用无钙灌流液灌流后,CNP仍能抑制Ach引起的收缩效应.而用胞内钙离子释放抑制剂(TMB-8)后,CNP不能抑制Ach引起的收缩效应.结论 CNP抑制Ach引起的胃平滑肌自发性收缩增强效应与胞内钙释放有关.     

皱皮木瓜总黄酮松弛胃肠平滑肌的效应机制

目的:探讨皱皮木瓜总黄酮(FLC)松弛胃肠平滑肌作用机制及其与Ca~(2 )的关系.方法:采用家兔离体空肠、回肠和结肠带生物测定法,在离体标本灌流槽中以Tyrode's液平衡家兔空肠、回肠和结肠带,分别观察FLC对空肠自律性收缩、对Ach诱导空肠收缩、对高钾所致结肠带收缩、以及CaCl2诱导回肠平滑肌收缩的影响.结果:FLC和Ver对高K~ 去极化所致结肠带收缩呈剂量依赖性松弛作用,压低最大效应(Emax);FLC(8g/L)对空肠自律性收缩(频率:5.83±2.64 vs 12.52±0.41,P<0.01;张力:0.76±0.26 g vs 2.13±0.21g,P<0.01)和Ach诱导的收缩反应(频率:7.00±2.44 vs 13.10±0.90,P<0.01;张力:0.87±0.34 g vs 3.47±0.57g,P<0.01)均具抑制作用;FLC剂量依赖性抑制Ca~(2 )诱导回肠收缩作用,4、8 g/L时收缩幅度显著低于对照组(2.53±0.45,1.35±0.57 mm vs 5.41±0.64mm,P<0.01).结论:FLC抑制胃肠平滑肌的功能与阻断电压依赖性的钙通道有关.     

莪术对大鼠结肠平滑肌收缩的促进作用及机制

目的:观察莪术对离体大鼠结肠平滑肌收缩运动的影响,并初步探讨其作用机制.方法:制备大鼠结肠平滑肌肌条,以9 g/LNaCl溶液(NS)为对照组,观察不同浓度莪术对结肠平滑肌的收缩效应;以莪术为对照组.分别观察酚妥拉明、维拉帕米和阿托品3种阻断剂孵育肌条后,莪术对肌条的收缩效应.结果:莪术高浓度组对离体大鼠结肠平滑肌的促进作用明显,与NS对照组比较,莪术1g/L、10 g/L组引起的肌条最大收缩振幅和曲线下面积均有统计学意义(63.92±2.06,76.27±2.28vs100%;44.09±11.10,55.66±10.29vs100%,P<0.05或<0.01);与单独莪术组比较,阻断剂维拉帕米和阿托品对莪术引起的肌条收缩有抑制作用,其最大收缩振幅和曲线下面积均有统计学意义(87.35%±50.49%,73.80%±9.37%vs 100%,33.97%±15.18%,27.55%±11.56% vs 100%,P<0.05或<0.01).结论:莪术对大鼠结肠平滑肌的收缩活动有兴奋作用,且与剂量呈正相关,其引起的收缩效应可被阿托品和维拉帕米阻断,而未能被酚妥拉明阻断.     

Regulation of the peptidergic nerves (substance P and vasoactive intestinal peptide) in the colon of women patients with slow transit constipation: an in vitro study

BACKGROUND/AIMS: In histological studies, there is evidence to suggest a diminution of the peptidergic nerves such as vasoactive intestinal peptide (VIP) and substance P (SP) in the enteric nervous system in the colon of patients with slow transit constipation (STC). To clarify the pathophysiological significance of peptidergic nerves in the colon of patients with STC, we investigated the enteric nerve responses on pathological and normal bowel segments derived from patients with STC and patients who underwent colon resection for colon cancers, respectively. METHODOLOGY: Twenty-eight preparations were taken from the pathological sigmoid colon of 16 women with STC (aged 40-58 years, average 48.8 years). Forty-eight preparations were taken from the normal sigmoid colon of 20 women with colonic cancer (aged 40-55 years, average 49.6 years). A mechanographic technique was used to evaluate in vitro muscle responses to VIP and SP of adrenergic and cholinergic nerves before and after treatment with various autonomic nerve blockers. RESULTS: Responses mediated by non-adrenergic non-cholinergic (NANC) inhibitory nerves were found in the normal colon, but were more frequently in the colon with STC than in the normal colon (p < 0.01). Responses mediated by excitatory nerves such as cholinergic nerves were more dominant in the normal colon than in the STC colon. At 1 x 10(-8), 1 x 10(-7), 1 x 10(-6) g/mL, VIP and SP in both the normal and STC colonic muscle strips produced a concentration-dependent relaxation to VIP and contraction to SP. In addition, the relaxation reaction to VIP in the colon with STC was also weaker than in the normal colon (p < 0.01). The contraction reaction to SP in the colon with STC was weaker than in the normal colon (p < 0.01). VIP acts through neural mechanisms, whereas SP may act both through nerves and also directly on both the normal and STC muscle strips. CONCLUSIONS: Responses mediated by NANC inhibitory nerves were significantly increased in the colon with STC compared with the normal colon. A decrease of responses to peptidergic nerves such as SP and VIP may also play an important role in the impaired motility observed in the colon of patients with STC. These results indicate that the disturbances in the neural component of the enteric nervous system in the colon of women patients with STC may initiate or contribute to the functional changes.     

一氧化氮在四磨汤诱导大鼠胃窦平滑肌收缩中的作用

背景：临床实践显示四磨汤具有全胃肠促动力效应。鉴于一氧化氮（NO）在胃肠神经介导胃肠平滑肌松弛中起重要中介作用,推测其可能参与了四磨汤对胃窦平滑肌收缩的调节。目的：研究NO在四磨汤诱导大鼠胃窦平滑肌收缩中的作用。方法：分别以梯度剂量（1~200μL）四磨汤和10-4mol/L NO供体左旋精氨酸（L-Arg）＋梯度剂量（1~200μL）四磨汤作用于大鼠离体胃窦纵肌条和环肌条,记录肌条基础状态和给药后收缩活动。结果：四磨汤能剂量依赖性地促进胃窦纵肌条和环肌条收缩（P=0.000）。经L-Arg预处理的胃窦纵、环肌条加入梯度剂量四磨汤后,肌条收缩活性量效曲线较单用四磨汤显著下移（L-Arg＋5~200μL四磨汤对单用5~200μL四磨汤,P均〈0.05）,表明NO可部分阻断四磨汤对胃窦平滑肌的兴奋效应。经L-Arg＋低中剂量（1~50μL）四磨汤作用的环肌条,收缩活性增幅显著低于纵肌条（P均〈0.05）。结论：四磨汤对大鼠胃窦平滑肌具有明显促收缩作用,该作用部分是通过抑制NO释放实现的。四磨汤对胃窦环肌的兴奋效应较纵肌更多依赖于抑制NO释放。     

白术对豚鼠结肠体外肌条运动的影响

[目的]观察白术对豚鼠体外结肠平滑肌运动的影响.[方法]制备豚鼠结肠纵肌和横肌肌条,放置在灌流肌槽内,用不同浓度白术水煎刺激,经张力换能器和生物信号采集系统,测量白术对横肌条和纵肌条运动积分和相对运动百分比的影响.[结果]不同浓度白术水煎剂使豚鼠横肌和纵肌运动积分和相对运动百分比均增强,对纵肌的作用强予横肌.[结论]白术水煎剂可增强结肠收缩,呈浓度依赖性,非双向调节作用.     

钙离子在大鼠结肠平滑肌运动中作用机制的研究

目的 应用束缚应激大鼠实验模型,研究离体结肠平滑肌的收缩运动及其影响因素,探讨鸟苷素在结肠运动中的作用。方法 建立束缚应激大鼠动物模型,制备离体结肠平滑肌环行肌及纵行肌肌条,应用张力换能器,测定其肌张力。应用放射配基法测定结肠组织及血浆中鸟苷素含量。结果 束缚应激刺激可诱发大鼠排便增加,该动物模型是较好的模拟人ＩＢＳ的实验动物模型。束缚应激大鼠离体结肠平滑肌的张力升高,对Ｋ＾＋、Ｃａ＾２＋、乙酰胆     

中药促结肠动力作用的筛选研究

目的 :探讨中药的促结肠动力作用 ,以筛选出具有开发价值的促结肠动力药物。方法 :制备大鼠结肠纵肌和横肌肌条 ,以 0 .85 %氯化钠溶液和乙酰胆碱为对照 ,观察元胡、香附、白芍、生白术、蒲公英、小茴香、大腹皮、大黄、莱菔子、厚朴、槟榔 11味中药对离体结肠肌收缩活性的效应。结果 :该 11味中药对肌条均有不同程度的兴奋效应 (P <0 .0 5 ) ,其中 ,槟榔、莱菔子、小茴香、生白术对横肌及纵肌均有显著性的兴奋作用 ,蒲公英对平滑肌肌条的兴奋作用以横肌较为显著 ,白芍作用较弱。结论 :槟榔、小茴香、蒲公英、莱菔子、生白术具有较强的促结肠动力作用 ,可以进一步研究开发。     

Human colonic smooth muscle: spontaneous contractile activity and response to stretch.

The length dependence of the spontaneous contractile activity of human colonic muscle was assessed in vitro. Muscle obtained from the right colon was more distensible than that of the left colon. This was true for all muscle layers. Maximum spontaneous active stress was exerted by both circular and longitudinal muscle layers of the right colon at greater degrees of stretch (p less than 0.001) than those of the left colon. The contractile frequency of longitudinally oriented strips increased with length. The contractile frequency of intertaenial longitudinally oriented strips from the right colon was lower (p less than 0.001) than that of strips from the left colon. The contractile frequency of circularly-oriented strips from the right colon (6.25 +/- 0.38 min) was higher (p less than 0.001) than that of strips from the left colon (3.35 +/- 0.35 min). The human colon appears to consist of two distinct areas based on the mechanical behaviour of the smooth muscle during spontaneous contraction.     

The differential effect of VIP and PACAP on guinea pig gallbladder in vitro

OBJECTIVES: Pituitary adenylate cyclase-activating polypeptide (PACAP) is a recently identified member of the secretin/glucagon/vasoactive intestinal peptide (VIP) family. Like VIP, PACAP is largely inhibitory in the gastrointestinal tract. The aim of our work was to characterize the effects of PACAP on both contraction and relaxation of guinea pig gallbladder (GPGB) muscle. METHODS: Gallbladder muscle strips were obtained from male Dunkin-Hartley guinea pigs (250-350 g). Isometric tension was measured in strips suspended in gassed (95% O2, 5% CO2) Krebs' solution at 37 degrees C and equilibrated for 60 min. Cumulative additions of VIP or PACAP (10(-9)-10(-6) mol/l) were performed with strips at basal tone or with strips pre-contracted with cholecystokinin-octapeptide (CCK-8). RESULTS: VIP had no effect on basal tone, in contrast with PACAP which produced concentration-dependent contraction to a maximum of 57.9 +/- 24.3% of control (CCK 3 x 10(-7) mol/l). The highest concentration (10(-6) mol/l) of VIP produced a 32 +/- 6% relaxation of 3 x 10(-9) mol/l CCK-8-contracted GPGB. With 3 x 10(-8) mol/l CCK-contracted GPGB strips, VIP produced a 26.7 +/- 6.6% relaxation. PACAP produced a further concentration-dependent contraction of 3 x 10(-9) mol/l CCK-contracted strips which reached 17.5 +/- 9.9% at the maximal concentration used (10(-6) mol/l). PACAP produced a concentration-dependent relaxation of 3 x 10(-8) mol/l CCK-contracted strips which reached a maximum relaxation of 19 +/- 5% of the control. CONCLUSIONS: PACAP has a dual effect on guinea pig gallbladder motility in vitro, producing contraction in the basal state, and both contraction and relaxation in the CCK-contracted state. This is in contrast to the effects of VIP, a closely related peptide.     

Impaired capsaicin and neurokinin-evoked colonic motility in inflammatory bowel disease

BACKGROUND: Inflammatory bowel disease (IBD) is associated with altered sensory and motor function in the human colon. The aim of the present study was to compare neuromuscular function in normal and IBD-affected colon in vitro, with emphasis on inhibitory enteric nerves, sensory neuropeptides and stimulation of axon collaterals. METHODS: Strips of longitudinal and circular muscle were prepared following colectomy from six patients with intestinal carcinoma (mean age 64.2 +/- 4.8 years) and six patients with IBD (Crohn's disease, n = 3; ulcerative colitis, n = 3: mean age 35.8 +/- 5.7 years). Responses were measured to electrical field stimulation, potassium chloride, 1,1-dimethyl-4-phenylpiperazinium iodide, isoprenaline, calcitonin gene-related peptide (CGRP), capsaicin and neurokinin (NK)-1 and -2 receptor subtype-specific agonists, alone or after muscle precontraction. RESULTS: The NK-1 and CGRP receptor-mediated relaxation was reduced in the circular (by 44%, P < 0.05) and longitudinal (by 61%, P < 0.05) muscle from IBD-affected colon, respectively. Maximal NK-2 receptor-mediated contraction was also significantly decreased in both longitudinal (71%, P < 0.001) and circular (51%, P < 0.01) muscle. Capsaicin evoked relaxation in precontracted colonic longitudinal and circular muscle; this was significantly diminished in the IBD-affected colon (by 63%, P < 0.001 and 76%, P < 0.01, respectively). Responses evoked by stimulation of enteric inhibitory nerves were not significantly altered. CONCLUSIONS: Colonic muscle strips from patients with IBD exhibited impaired CGRP and NK-1 receptor-mediated relaxation and NK-2 receptor-mediated contraction. Capsaicin-activated relaxation of colonic smooth muscle is deficient in IBD-affected colon. These results suggest a discrete effect of IBD on sensory-motor coupling and tachykinin-mediated effects on colonic motility.     

Effect of areca on contraction of colonic muscle strips in rats

AIM: To investigate the effects of areca on the contractileactivity of isolated colonic muscle strips in rats andmechanism involved.METHODS: Each strip (LMPC, longitudinal muscle ofproximal colon; CMPC, circular muscle of proximal colon;LMDC, longitudinal muscle of distal colon; CMlC, circularmuscle of distal colon. ) was suspended in a tissue chambercontaining 5 mL Krebs solution (37 ℃), bubbledcontinuously with 950 mL@ L-1 O2 and 50 mL@ L-1 CO2 . Themean contractile amplitude (A), the resting tension (T),and the contractile frequency (F) were simultaneouslyrecorded on recorders.RESULTS: Arsca dose dependently increased the meancontractile amplitude, the resting tension of proximal anddistal colonic smooth muscle strips in rats ( P ＜ 0.05). Italso partly increased the contractile frequency of colonicsmooth muscle strips in rats ( P ＜ 0.05). The effects werepartly inhibited by atropine (the resting tension of LMPCdecreased from 0. 44 ± 0. 12 to 0. 17 ± 0.03; the restingtension of LMDC decreased from 0.71 ± 0.14 to 0.03 ± 0.01;the mean contractile amplitude of LMPC increased from -45.8 ± 7.2 to -30.5 ± 2.9; the motility index of CMDC decreasedfrom 86.6± 17.3 to 32.8 ± 9.3; P＜ 0.05 vs areca), but theeffects were not inhibited by hexamethonium (P＞ 0.05).CONCLUSION: Areca stimulated the motility of isolatedcolonic smooth muscle strips in rats. The stimulation ofareca might be relevant with M reoeptor partly.