3株肝细胞在硝酸纤维素膜界面上生长的初步研究

目的在体外培养的条件下,观察3株肝细胞在硝酸纤维素膜材料上的生长情况,研究膜的细胞相容性及理化性能的改变,初步探讨硝酸纤维素膜作为一种新的组织工程膜材料用于构建生物人工肝生物反应器中细胞的载体材料的可行性。方法将生物人工肝常用的细胞：肝肿瘤细胞HepG2、C3A、永生化HL7702细胞株分别接种在浸泡处理后的硝酸纤维素膜片上常规培养,在普通光学显微镜以及扫描电镜观察肿瘤细胞在此种膜上的贴壁情况和细胞形态。同时行免疫细胞化学染色和细胞功能检测。结果细胞经常规染色、透明处理后,在光镜下观察到膜上生长的细胞保持了较好的增殖状态及细胞形态;免疫细胞化学染色结果表明,膜上细胞特异性标志物表达良好。培养液上清的生化检测表明培养细胞的主要分泌、代谢功能与常规培养无明显差异;扫描电镜下膜表面细胞呈集落式三维生长,增殖明显。结论硝酸纤维素膜能促进细胞黏附,并且与细胞有良好的相容性。硝酸纤维素膜作为一种良好的细胞黏附生长介质的同时,还具有可以在其上面直接进行相关培养物观察和检测的优点,有望作为一种新的细胞培养介质和生物人工肝反应器中新的膜材料     

晚期内皮祖细胞在纳米聚-L-乳酸支架膜表面的粘附和增殖

目的通过比较晚期内皮祖细胞在纳米无序及有序聚-L-乳酸支架膜表面粘附、增殖情况,为优化组织工程材料提供一种新途径。方法通过静电纺丝技术制备纳米聚-L-乳酸纤维支架,行低温等离子体技术改性及I型胶原表面涂覆,与晚期内皮祖细胞复合培养,并进行细胞双荧光染色鉴定,通过不同时间点两组材料晚期内皮祖细胞粘附和增殖率的测定比较及光镜、荧光显微镜和扫描电镜观察支架材料对种子细胞形态特征等方面的影响。结果制得的纳米聚-L-乳酸纤维孔径在300～400nm之间,孔隙率>90%;细胞粘附12h基本完成;各时间点有序膜组增殖率明显高于无序膜组(P<0.05)。细胞在支架膜上生长良好,无序膜组细胞生长较散在、杂乱;有良好空间定向效果的有序纤维支架有利于细胞沿纤维定向附着、伸展、增殖,分泌胞外基质。结论晚期内皮祖细胞是理想的组织工程种子细胞来源;纳米聚-L-乳酸有序膜支架能促进种子细胞在材料表面的粘附、增殖,并能较好地保持细胞形态,是一种理想的血管组织工程支架材料。     

聚乳酸-聚乙醇酸共聚物与心肌样细胞生物相容性的研究

目的: 探讨聚乳酸-聚乙醇酸(PLGA)与骨髓间充质干细胞(BM-MSCs)衍生的心肌样细胞的生物相容性,为构建心肌组织工程及其进一步体内回植提供研究基础。方法: 以密度梯度离心法体外分离培养SD大鼠的BM-MSCs,将培养的第3 代细胞用终浓度为10 μmol/L的5-氮杂胞苷和0.1μmol/L的血管紧张素Ⅱ诱导分化。将诱导后的心肌样细胞接种到PLGA材料上, 用沉淀法测定细胞的黏附力和黏附率;MTT比色法检测细胞的增殖并绘制增殖曲线;扫描电镜观察细胞在PLGA材料上的形态学特征及细胞与该材料的相容性。结果: 心肌样细胞在PLGA支架材料上贴附、生长良好,可分泌细胞外基质,其黏附、增殖能力与单纯的细胞相比无显著统计学差异。结论: PLGA与心肌样细胞具有良好的生物相容性,可作为心肌样细胞的理想载体构建心肌组织工程。     

鼠骨髓间充质干细胞诱导的成骨细胞与3-羟基丁酸/3-羟基己酸共聚物的体外相容性

目的探讨SD大鼠骨髓间充质干细胞(BMSCs)诱导的成骨细胞与3-羟基丁酸/3-羟基己酸共聚物(PHBHHx)支架材料的体外相容性,进一步验证PHBHHx材料作为骨组织工程支架材料的可行性。方法将SD大鼠BMSCs诱导的成骨细胞与PHBHHx支架材料体外复合培养,通过扫描电镜观察细胞与材料的复合情况,用MTT法检测材料对细胞增殖的影响。结果成骨细胞在PHBHHx支架材料表面及孔隙中能较好地黏附和增殖。结论 PHBHHx与成骨细胞有良好的体外相容性,是一种性能优良的骨组织工程支架材料。     

重组人β-趋化因子RANTES抗T嗜性HIV-1作用的研究

目的　检测重组人β 趋化因子RANTES(RegulationuponactivationnormalT cellexpressedandsecreted)对T嗜性HIV 1病毒株SF 33在T细胞中复制的影响。方法　在SF 33感染细胞前 1h ,用不同浓度 (5ng/ml、5 0ng/ml、5 0 0ng/ml)的重组人RANTES、GST RANTES或TEP RANTES对MT 4细胞进行预处理。病毒感染后第6天 ,以ELISA方法检测p2 4抗原水平 ,MTT比色法检测感染细胞的存活数。结果　在不同浓度下 ,天然RANTES及修饰RANTES对SF 33p2 4水平不产生显著影响 ,半数抑制浓度IC50 >5 0 0ng/ml,对感染细胞的保护率在 0以下。结论　T嗜性HIV 1病毒株SF 33对RANTES及其修饰蛋白的抑制作用不敏感。     

低密度脂蛋白对体外培养的肾小球系膜细胞表型转化的影响

目的研究低密度脂蛋白(LDL)对体外培养的大鼠肾小球系膜细胞(MCs)表型转化的诱导作用。方法采用不同浓度的LDL(O，25，50，100，150μg／mL)刺激体外培养的大鼠肾小球系膜细胞的方法，以MTT比色法检测细胞增殖，透射电镜观察系膜细胞的形态学变化，免疫细胞化学染色及Western blot检测α-平滑肌肌动蛋白(α-SMA)、结缔组织生长因子(CTGF)、Ⅳ型胶原(ColⅣ)及纤维连接蛋白(FN)的表达。结果在LDL作用下，系膜细胞形态发生明显变化，细胞变为长梭形，透射电镜下出现肌动蛋白样结构。免疫细胞化学染色及Western blot显示α-SMA、CTGF、ColⅣ及FN表达增强(P＜0．05)。结论LDL具有诱导肾小球系膜细胞发生表型转化的作用。     

低密度脂蛋白对体外培养的肾小球系膜细胞表型转化的影响

目的研究低密度脂蛋白(LDL)对体外培养的大鼠肾小球系膜细胞(MCs)表型转化的诱导作用.方法采用不同浓度的LDL(0, 25, 50, 100, 150 μg/mL)刺激体外培养的大鼠肾小球系膜细胞的方法,以MTT比色法检测细胞增殖,透射电镜观察系膜细胞的形态学变化,免疫细胞化学染色及Western blot检测α-平滑肌肌动蛋白(α-SMA)、结缔组织生长因子(CTGF)、Ⅳ型胶原(ColⅣ)及纤维连接蛋白(FN)的表达.结果在LDL作用下,系膜细胞形态发生明显变化,细胞变为长梭形,透射电镜下出现肌动蛋白样结构.免疫细胞化学染色及Western blot显示α-SMA、CTGF、ColⅣ及FN表达增强(P<0.05).结论 LDL具有诱导肾小球系膜细胞发生表型转化的作用.     

阿魏酸钠对人EBL—7404肝癌细胞的抑制作用

目的观察阿魏酸钠(sodium ferulate,SF)对离体肝癌细胞生长的抑制作用.方法将人EBL-7404肝癌细胞以1×108/l浓度接种于25cm2细胞培养瓶中,接近单层时,分为SF组和对照组.SF组用含SF(终浓度为1mg/l)和小牛血清(50ml/l)的1640培养液培养细胞,对照组用含小牛血清(50ml/l)的1640培养液培养细胞,48h后,收集细胞,碘化丙啶(propidium iodide,PI)染色后,用流式细胞仪检测每一细胞周期的DNA含量,并根据DNA的含量分类细胞.结果(1)SF组细胞凋亡为10.5%,对照组为5.9%,凋亡被上调.(2)SF组DNA百分比在S期明显降低(SF组为33.12%,对照组为47.83%),在G2/M期明显增加(SF组为27.87%,对照组为15.04%).结论SF诱导了人EBL-7404肝癌细胞凋亡,抑制了人EBL-7404肝癌细胞的增殖,SF能是一种有效抑制肝癌生长的中药.     

H2O2诱导体外培养的皮肤成纤维细胞氧化应激损伤

目的探讨H2O2对体外培养的人皮肤成纤维细胞生物学特性的影响,从细胞水平为抗皮肤衰老药物的研究提供较理想的皮肤衰老模型。方法用不同浓度的H2O2(50～300μmol/L)干预体外培养的正常皮肤成纤维细胞,MTT比色法检测细胞增殖,流式细胞仪检测细胞凋亡,羟脯氨酸比色法检测胶原含量,紫外分光光度计测定细胞中超氧化物歧化酶(SOD)的活性和丙二醛(MDA)的含量。结果 H2O2浓度依赖性地抑制皮肤成纤维细胞增殖,促进细胞凋亡,降低细胞SOD的活性,减少细胞胶原含量,增加MDA含量。结论氧化应激可损伤皮肤成纤维细胞,用H2O2致皮肤成纤维细胞氧化损伤模型可用于抗皮肤衰老药物的研究。     

纳米羟基磷灰石人工心脏机械瓣膜细胞相容性的研究

目的探讨利用脉冲激光沉积（PLD）方法在人工心脏机械瓣膜上沉积的纳米相羟基磷灰石（HA）薄膜的组织相容性,为人工心脏瓣膜的构建提供理想的支架薄膜材料。方法将传代的人血管内皮细胞制成细胞悬液接种在HA材料上,置含5％（V／V）CO2、37℃的恒温培养箱内静态培养3周。分别在培养3、7、14和21d后取出部分材料,用2．5％戊二醛固定,乙醇梯度脱水,临界点干燥、喷金,于扫描电子显微镜（SEM）下观察细胞在材料上的附着情况。用四氮唑盐比色（MTT）法测定血管内皮细胞与材料复合培养的增殖能力。结果扫描电镜观察复合培养,见HA材料的表面有细胞附着且数目逐渐增多,到第21天时可见细胞融合成片,并将材料表面覆盖,部分区域有细胞外基质形成。MTT法评价材料对血管内皮细胞的细胞毒性,见细胞增殖未受到明显影响,生长良好。结论HA对血管内皮细胞的增殖和分化功能无明显影响,对细胞无明显毒性,具有良好的生物相容性,可以作为心脏瓣膜材料安全应用。     

Apelin促进脂肪间充质干细胞增殖、分化修复小鼠下肢缺血损伤的实验研究

目的:探讨apelin对于脂肪间充质干细胞(AD-MSCs)修复小鼠后肢缺血损伤的作用及机制。方法: 从β-actin-luc转基因小鼠中分离出表达荧光素酶的AD-MSCs,将20只近交系无报告基因的小鼠随机分为两组,即实验组:后肢注射AD-MSCs+apelin(n=10)和对照组:后肢注射AD-MSCs(n=10)。所有小鼠均结扎股动脉建立后肢缺血模型,股四头肌内注射AD-MSCs(1×106)或AD-MSCs+apelin,激光多普勒成像观察下肢血液灌注,生物发光成像检测细胞的活性。用体外培养的β-actin-luc转基因小鼠AD-MSCs,建立缺氧（6 h）模型。实验分为4组,即对照组、缺氧组、缺氧＋apelin干预组及缺氧＋apelin+LY294002组。用免疫印迹法检测细胞内激酶磷酸化Akt 的表达。结果: 在移植后1周内生物发光成像显示,AD-MSCs移植后存在急性死亡,加用apelin组中细胞的存活时间明显延长。离体实验显示,在缺氧环境中apelin干预AD-MSCs后,细胞pAkt的表达明显高于对照组(P<0.05),加入Akt阻断剂LY294002后,细胞磷酸化的水平降低(P<0.05)。结论: apelin对AD-MSCs的后肢缺血治疗具有保护作用,可能在缺血性疾病的治疗中成为重要的干预靶点。     

肝孤核受体激动剂对间充质干细胞的抗缺氧复氧损伤作用及机制

目的探讨肝孤核受体(LXR)激动剂对脂肪间充质干细胞(AD-MSCs)缺氧损伤的保护作用及可能机制。方法酶消化法分离稳定表达萤火虫荧光素酶(Fluc)报告基因的小鼠AD-MSCs,流式细胞术检测CD90、CD44、CD34、CD45细胞表面标记物。3代AD-MSCs分为7组:对照组;缺氧6 h/复氧2 h组(缺氧复氧组);缺氧/复氧+DMSO组(DMSO组);缺氧复氧+不同浓度LXR激动剂T0901317干预组(1μmol/L组、5μmol/L组、10μmol/L组、15μmol/L组)。运用Fluc报告基因生物发光成像技术对AD-MSCs细胞增殖进行定量评价,免疫印迹法检测细胞NF-κB表达水平,ELISA法检测AD-MSCs的白细胞介素6(IL-6)、TNF-α分泌水平。结果流式细胞术结果显示.AD-MSCs呈CD44(+)、CD90(+)、CD34(-)、CD45(-)。光学成像结果显示,AD-MSCs细胞数与Fluc平均生物发光信号强度呈正相关(r~2=0.97)。与对照组比较,缺氧复氧组AD-MSCs分泌TNF-α、IL-6、NF-κB明显升高;与缺氧复氧组比较,10μmol/L组AD-MSCs分泌TNF-α、IL-6、NF-κB明显减少(P<0.05,P<0.01)。结论 LXR激动剂可以促进缺氧复氧损伤后AD-MSCs的存活,并能通过NF-κB信号途径抑制炎性因子IL-6、TNF-α的释放,可为干细胞移植治疗心肌缺血再灌注损伤提供新的细胞保护方法。     

Sustainable Rabbit Skin Glue to Produce Bioactive Nanofibers for Nonactive Wound Dressings

This paper assessed the collagen glue (Col) from rabbit skin for use as a raw material in combination with different water-based dispersants of antimicrobial agents such as ZnO NPs, TiO₂ NPs doped with nitrogen and Ag NPs (TiO₂-N-Ag NPs), and chitosan (CS) for the production of biocompatible and antimicrobial nanofibers. The electrospun nanofibers were investigated by scanning electron microscopy (SEM), attenuated total reflectance in conjunction with Fourier-transform infrared spectroscopy (ATR-FT-IR) analyses and antioxidant activity. The biocompatibility of electrospun nanofibers was investigated on cell lines of mouse fibroblast NCTC (clone L929) using MTT test assays. Antimicrobial activity was performed against Escherichia coli and Staphylococcus aureus bacteria and Candida albicans pathogenic fungus. Electrospun antimicrobial nanofibers based on collagen glue achieved reduction in the number of viable microorganisms against both fungi and bacteria and exhibited multiple inhibitory actions of fungal and bacterial strains. The electrospun nanofibers showed average dimension sizes in the range of 30–160 nm. The results indicated that both Col/TiO₂-N-Ag NPs and Col/CS formulations are suitable for cell proliferation and may be useful for producing of nonactive wound dressings.     

Synthesis and Characterization of Electrospun Composite Scaffolds Based on Chitosan-Carboxylated Graphene Oxide with Potential Biomedical Applications

This research study reports the development of chitosan/carboxylated graphene oxide (CS/GO-COOH) composite scaffolds with nanofibrous architecture using the electrospinning method. The concept of designed composite fibrous material is based on bringing together the biological properties of CS, mechanical, electrical, and biological characteristics of GO-COOH with the versatility and efficiency of ultra-modern electrospinning techniques. Three different concentrations of GO-COOH were added into a chitosan (CS)-poly(ethylene oxide) (PEO) solution (the ratio between CS/PEO was 3/7 (w/w)) and were used in the synthesis process of composite scaffolds. The effect of GO-COOH concentration on the spinnability, morphological and mechanical features, wettability, and biological properties of engineered fibrous scaffolds was thoroughly investigated. FTIR results revealed the non-covalent and covalent interactions that could take place between the system’s components. The SEM micrographs highlighted the nanofibrous architecture of scaffolds, and the presence of GO-COOH sheets along the composite CS/GO-COOH nanofibers. The size distribution graphs showed a decreasing trend in the mean diameter of composite nanofibers with the increase in GO-COOH content, from 141.40 nm for CS/PG 0.1% to 119.88 nm for CS/PG 0.5%. The dispersion of GO-COOH led to composite scaffolds with increased elasticity; the Young’s modulus of CS/PG 0.5% (84 ± 4.71 MPa) was 7.5-fold lower as compared to CS/PEO (662 ± 15.18 MPa, p < 0.0001). Contact angle measurements showed that both GO-COOH content and crosslinking step influenced the surface wettability of scaffolds, leading to materials with ~1.25-fold higher hydrophobicity. The in vitro cytocompatibility assessment showed that the designed nanofibrous scaffolds showed a reasonable cellular proliferation level after 72 h of contact with the fibroblast cells.     

Biochemical and molecular characterization of hepatocyte-like cells derived from human bone marrow mesenchymal stem cells on a novel three-dimensional biocompatible nanofibrous scaffold

Background:  There is significant interest in using nanofibers in tissue engineering from stem cells. The transdifferentiation of mesenchymal stem cells into the hepatic lineage in a nanofibrous structure has not been reported. In this study, a three dimensional nanofibrous scaffold is introduced for differentiation of human bone marrow derived mesenchymal stem cells (hBMSCs) into hepatocytes.
Methods:  A scaffold composed of Poly (ε-caprolactone), collagen and polyethersulfone was fabricated by the electrospinning technique. After characterization of isolated hBMSCs, the performance of the cells on the scaffold was evaluated by Scanning Electron Microscopy (SEM) and MTT assay. Cytological, molecular and biochemical markers were measured to confirm differentiation potential of hBMSCs into hepatocytes.
Results:  The isolated cells possessed the basic properties of mesenchymal stem cells (MSCs). Based on scanning electron microscope (SEM) analysis and MTT assay, it was shown that the cells adhere, penetrate and proliferate on the nanofibers. Cultured cells on the nanofibers differentiated into hepatocyte-like cells and expressed hepatocyte specific markers such as albumin, α-fetoprotein, cytokeratin-18, cytokeratin-19 and cytochrome P450 3A4 at mRNA levels. Appearance of a considerable number of albumin-positive cells cultivated on the scaffold (47 ± 4%) as compared to the two-dimensional culture system (28 ± 6%) indicates the supporting role of the scaffold. The efficiency of the cells to produce albumin, urea, transferrin, serum glutamic pyruvic transaminase and serum oxaloacetate aminotransferase in hepatocytes on the scaffold further attest to the functionality of the cells.
Conclusion:  The data presented in this study show that the engineered nanofibrous scaffold is a conductive matrix which supports and enhances MSC development into functional hepatocyte-like cells.     

Influence of Chemical Pre-Treatments and Ultrasonication on the Dimensions and Appearance of Cellulose Fibers

Due to the wider use of nanocellulose in various areas of economic life, better and more optimal methods of obtaining nanocellulose are constantly being sought. Therefore, an attempt was made to evaluate the hybrid cellulose treatment, based on the use of a chemical method combined with an ultrasound of medium frequency. The study employs two different starting materials (Södra Black R cellulose or microcrystalline cellulose), two types of chemical pre-treatments (acid hydrolysis or oxidation), and two sonication durations. It was found that the reduction fiber cross-sectional dimensions was the result of prolonged exposure of cellulose to the ultrasound. From Södra Black R and the microcrystalline cellulose nanometer scale, structures were obtained in the form of isolated fibers. The TEMPO reagent accelerated the degradation process of two cellulose varieties due to its oxidizing character. The resulting products had nanofibrous structures. Cellulose degradation as a result of the combined action of sonication and TEMPO activity progressed gradually. Places of fiber degradation were characterized by their longitudinal breakage and initiated the next stages of the defibering process.     

In vitro and in vivo characterization of silk fibroin/gelatin composite scaffolds for liver tissue engineering

OBJECTIVE: To investigate the cytotoxicity of silk fibroin/gelatin (SF/G) composite scaffolds in vitro as well as their biocompatibility and degradation in vivo. METHODS: The proliferation and relative growth rate of human hepatic QZG cells grown on different blends of two‐dimensional (2‐D) SF/G scaffolds were assessed using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. Flow cytometry was used to evaluate apoptotic rate of QZG cells on different blends of 2‐D SF/G scaffolds. The effect of silk protein materials on cell growth was observed by scanning electron microscopy. Three‐dimensional (3‐D) SF/G scaffolds of three different ratios (diameter 10 mm, thickness 1 mm) were implanted into subcutaneous pockets on male Sprague–Dawley (SD) rats. On the 7th, 14th and 30th day post‐implantation, the rats were sacrificed. The scaffold area including the surrounding tissues was retrieved. Hematoxylin and eosin staining was performed for observation under a light microscope. RESULTS: Significant cell attachment and proliferation on the SF/G scaffolds were observed. As the increased gelatin concentration, SF/G scaffolds became more amenable to cell adhesion. After the subcutaneous implantation of the SF/G scaffolds in SD rats, immunological rejection tests showed only slight inflammation, measured by the presence of inflamed cells on day 7 and 14. By day 30, each scaffold had been completely infiltrated and organized by fibroblasts and inflamed cells. The greater the gelatin concentration in the scaffold, the faster the degradation rate. CONCLUSION: Composite SF/G scaffolds are a promising candidate matrix for implantable bio‐artificial livers.     

Bioactive Hydrogels: Design and Characterization of Cellulose-Derived Injectable Composites

Cellulose represents a low cost, abundant, and renewable polysaccharide with great versatility; it has a hierarchical structure composed of nanofibers with high aspect ratio (3–4 nm wide, hundreds of μm long). TEMPO-mediated oxidation represents one of the most diffused methods to obtain cellulose nanofibers (CNFs): It is possible to obtain physically crosslinked hydrogels by means of divalent cation addition. The presence of inorganic components, such as calcium phosphates (CaP), can improve not only their mechanical properties but also the bioactivity of the gels. The aim of this work is to design and characterize a TEMPO-oxidized cellulose nanofibers (TOCNFs) injectable hydrogel embedded with inorganic particles, CaP and CaP-GO, for bone tissue regeneration. Inorganic particles act as physical crosslinkers, as proven by rheological characterization, which reported an increase in mechanical properties. The average load value registered in injection tests was in the range of 1.5–4.4 N, far below 30 N, considered a reasonable injection force upper limit. Samples were stable for up to 28 days and both CaP and CaP-GO accelerate mineralization as suggested by SEM and XRD analysis. No cytotoxic effects were shown on SAOS-2 cells cultured with eluates. This work demonstrated that the physicochemical properties of TOCNFs-based dispersions could be enhanced and modulated through the addition of the inorganic phases, maintaining the injectability and bioactivity of the hydrogels.     

Biodegradable Nanofibrous Membranes for Medical and Personal Protection Applications: Manufacturing,Anti-COVID-19 and Anti-Multidrug Resistant Bacteria Evaluation

Biodegradable nanofibrous hybrid membranes of polyvinyl alcohol (PVA) with ZnO and CuO nanoparticles were manufactured and characterized, and their anti-COVID-19 and anti-multidrug resistant bacteria activities were also evaluated. The morphological structures of the prepared PVA composites nanofibers were observed by scanning electron microscope (SEM), which revealed a homogenous pattern of the developed nanofibers, with an average fibrous diameter of 200–250 nm. Moreover, the results of the SEM showed that the fiber size changed with the type and the concentration of the metal oxide. Moreover, the antiviral and antibacterial potential capabilities of the developed nanofibrous membranes were tested in blocking the viral fusion of SARS-COV-2, as a representative activity for COVID-19 deactivation, as well as for their activity against a variety of bacterial strains, including multi-drug resistant bacteria (MDR). The results revealed that ZnO loaded nanofibers were more potent antiviral agents than their CuO analogues. This antiviral action was attributed to the fact that inorganic metallic compounds have the ability to extract hydrogen bonds with viral proteins, causing viral rupture or morphological changes. On the other hand, the anti-multi-drug resistant activity of the prepared nanofibers was also evaluated using two techniques; the standard test method for determining the antimicrobial activity of immobilized antimicrobial agents under dynamic contact conditions and the standard test method for determining the activity of incorporated antimicrobial agents in polymeric or hydrophobic materials. Both techniques proved the superiority of the ZnO loaded nanofibers over the CuO loaded fibers. The results of the antiviral and antibacterial tests showed the effectiveness of such nanofibrous formulas, not only for medical applications, but also for the production of personal protection equipment, such as gowns and textiles.