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胶体金免疫民镜技术是利用抗原和抗体特异性结合,在电镜下检测与抗体结合电子密度高的胶体金,确定抗原位置。因此已成为研究细胞化学的重要技术,得到广泛应用。但被检的胶体金粒子直径一般在10nm左右,很难在低倍下、大视野观察不同细胞的标记情况,为此,增大胶体金颗粒十分必要。 相似文献
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应用分级抽提方法~[1],结合整装细胞电镜技术(Whole mount ceu EM)、非树脂包埋——去包埋剂电镜技术(embedment—free EM)~[2]及扫描电镜技术对HeLa细胞和牛肾传代细胞的中间纤维体系进行了观察,同时还应用角蛋白(Keratin)单克隆抗体——胶体金免疫电镜技术对HeLa细胞的中间纤维进行了定性研究。中间纤维在整装细胞电镜、DGD(Diethylene Glycol Distearate)包埋——去包埋剂电镜及扫描电镜下,均呈致密网状,其单丝直径均约10nm,这说明中间纤维在非离子去垢剂如Triton x—100及(NH_4)_2SO_4溶液的分级提取过程中是稳定的;同时也可以观察到较粗的纤维,这显然是单丝聚合形成的束。 相似文献
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用胶体金显示PbGv在脂肪体中的免疫化学定位 总被引:1,自引:0,他引:1
胶体金作为标记物最早由 Faulk和 Toylor( 1 971 ) Romano等 ( 1 974)用于电镜免疫细胞化学研究。近年来胶体金探针已成为定位和分析多种蛋白质、多肽、多糖的有力工具。特别是在抗原酶及激素等物质的细胞免疫化学定位中更加显示了其独特的威力。利用 Ig G的双价性 ,它的一臂可结合到事先包被有抗原的胶体金上 ,也可以把抗体分子包被在胶体金上 ,对细胞内的抗原进行定位。昆虫病毒在感染细胞中有很强的能力。其中杆状病毒包含体蛋白的复制量可达到死虫的2 0 %是当前所了解的真核细胞中表达量最大的病毒蛋白质。为了了解这蛋白合成和代谢的… 相似文献
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胶体金免疫电镜技术具有步骤简便。胶体金探针能精确定位、电镜下分辨率高等优点,给细胞化学标记体系带来了一种改革,近年来越来越多地应用于生物学和医学研究中,如:细胞内某些生物活性物质的定位研究、组织细胞形态发生研究、酶的研究等,但在农业科研中的应用还很少。尤其在病毒检测方面,目前国内只见到以胶体金免疫电镜技术检测动物病毒的报道,而用于植物病毒检测的报道尚无。本文报道了以改良的柠檬酸钠—鞣酸还原法制备大小适于植物病毒标记的胶体金颗粒的方法,制备的胶 相似文献
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应用胶体金免疫电镜技术对肝癌、癌旁肝及正常肝细胞的整合素β1亚族粘附受体表达、分布情况进行了观察。结果显示,正常肝细胞、内皮细胞呈现阳性表达。肝癌细胞也有少量阳性表达。癌旁肝细胞阳性表达明显增多。本文并对肝细胞和内皮细胞中粘附受体的表达与肿瘤转移及胶原纤维形成关系进行了讨论。 相似文献
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采用LR White作为包埋剂,用胶体金标记包埋后直接和间接免疫电镜技术,对天疱疮、大疱性类天疱疮、线型IgA大疱性皮肤病皮损组织中的抗体和血清中自身抗体对正常人皮肤的结合部位进行研究。发现IgG型天疱疮抗体位于桥粒上;IgG型类天疱疮抗体对半桥粒上及透明层中;而线型IgA大疱病IgA型自身抗体结合在半桥位、透明层和致密层中。提示寻常型天疱疮和落叶型天疱疮抗原均位于桥粒复合体上;大疱性类天疱疮抗原位于半桥粒上及其下方的透明层虽;线型IgA大疱病抗原位于半桥粒上、透明层和、或致密层中。本文同时就金标记包埋后免疫电镜在大疱性皮肤病诊断和研究中的价值进行介绍了讨论。 相似文献
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以2006年12月13日射电爆发事件为例, 利用国际GNSS服务(international GNSS service, IGS)观测台网数据, 结合L波段太阳射电流量和幅度闪烁指数监测数据, 研究了向阳面地基台站对这次射电暴的响应.重点分析了我国境内几个典型地区台站载噪比的变化特征, 并评估此次事件对不同地区GNSS服务和定位精度的影响程度.结果表明, 太阳射电暴期间:1)GNSS信号受到严重干扰, 几乎所有向阳面IGS台站都受到影响, 影响程度呈现出明显的随太阳入射角变化特征; 2)此次事件造成多个IGS台站锁定卫星数目小于4颗, 大面积连续性失锁、间歇性失锁和导航信号完全中断现象发生; 3)中国境内多个台站的定位误差显著增加, 伪距单点定位误差高达40~60 m, 精密单点定位(precise point positioning, PPP)误差达到2 m左右, 一些台站发生短时定位服务中断; 4)这次事件给日下点附近的台站造成非常严重的影响, PPP误差超过10 m, 而且还出现长达约10 min的定位服务中断.文中结果能为第三代北斗导航卫星系统在评估空间天气威胁方面提供借鉴和参考. 相似文献
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Otake Y Armand M Armiger RS Kutzer MD Basafa E Kazanzides P Taylor RH 《IEEE transactions on medical imaging》2012,31(4):948-962
Intraoperative patient registration may significantly affect the outcome of image-guided surgery (IGS). Image-based registration approaches have several advantages over the currently dominant point-based direct contact methods and are used in some industry solutions in image-guided radiation therapy with fixed X-ray gantries. However, technical challenges including geometric calibration and computational cost have precluded their use with mobile C-arms for IGS. We propose a 2D/3D registration framework for intraoperative patient registration using a conventional mobile X-ray imager combining fiducial-based C-arm tracking and graphics processing unit (GPU)-acceleration. The two-stage framework 1) acquires X-ray images and estimates relative pose between the images using a custom-made in-image fiducial, and 2) estimates the patient pose using intensity-based 2D/3D registration. Experimental validations using a publicly available gold standard dataset, a plastic bone phantom and cadaveric specimens have been conducted. The mean target registration error (mTRE) was 0.34 ± 0.04 mm (success rate: 100%, registration time: 14.2 s) for the phantom with two images 90° apart, and 0.99 ± 0.41 mm (81%, 16.3 s) for the cadaveric specimen with images 58.5° apart. The experimental results showed the feasibility of the proposed registration framework as a practical alternative for IGS routines. 相似文献
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A. A. Titov D. V. Solomentsev V. U. Khattatov 《Journal of Communications Technology and Electronics》2013,58(6):509-516
The 3D assimilation ionospheric model is improved to assimilate in real time the ionospheric total electron content (TEC) measurement data from the International GNSS Service (IGS) network of ground-based stations of the global positioning system (GPS). This model makes it possible to calculate the space-time electron-concentration distributions of electrons, concentration of the seven main ions, and the temperature and velocity of electrons and ions in the ionosphere at altitudes of 100–1000 km. The model calculations of the ionospheric TEC are compared to the TEC measured on slant paths with the use of two-frequency receivers of the ground-based IGS network of stations not included into the assimilation scheme. The model calculations of ionospheric electron-concentration height profiles are compared to the data measured by an incoherent-scatter radar. It is shown that the ionospheric parameters calculated without using experimental data are in worse agreement with the radar measurement data than the results obtained with the assimilation model of the ionosphere. The model-calculated electron concentrations are compared to the data from the FORMOSAT-3/COSMIC system of medium-Earth-orbit satellites. 相似文献
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The author requests a more precise indication of the link between this theorem and the Ralston (1962) symmetric matrix and Routh-Hurwitz determinants. 相似文献
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Anique Scheerder Alexander van Deursen Jan van Dijk 《Telematics and Informatics》2017,34(8):1607-1624
Recently, several digital divide scholars suggested that a shift is needed from a focus on binary Internet access (first-level digital divide) and Internet skills and use (second-level digital divide) to a third-level digital divide in which the tangible outcomes of Internet use are highlighted. A plethora of studies have been conducted to identify determinants of digital divides. Unfortunately, there is a lack of consistency in the terminology used. Moreover, terms are often not theoretically grounded. Therefore, we conducted a systematic literature review of digital divide determinants. The results show that the third-level digital divide was underexposed. The primary focus is on Internet use. More importantly, the identified determinants show that digital divide research is largely limited to sociodemographic and socioeconomic determinants. 相似文献
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精密钟差以及天顶对流层时延是GPS PPP解算的重要参数.本文通过在PPP数据处理过程中加入基于更新迭代的状态协方差阵作为取权因子,分别在高精度时间比对和天顶对流层时延方面利用单向滤波以及双向加权滤波对PPP解算的钟差及对流层时延进行估计与分析.结果表明,在零基线比对方面,经加权估计的钟差标准偏差优于单向结果,提高在30%以上;在时间比对方面,利用加权估计的钟差与国际权度局发布的钟差在频率稳定度上水平相当;最后,在天顶对流层时延估计方面,两种方法相对于IGS分析中心公布的天顶对流层时延的残差均方根误差中,加权估计的天顶对流层时延也略有提高. 相似文献
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Iu N Zueva 《Eksperimental?nai?a onkologii?a》1984,6(3):33-36
Using the enzyme-labelled antibodies it was shown that extracts from leukocytes of patients with leukemias and healthy donors contain antigenic determinants related to major virus protein of mammalian C type oncornaviruses SSV/SSAV, BaEV, FeLV and RLV. The highest antigen activity in patients was detected in the feline leukemia (FeLV) system, and the least one--in the system of murine leukemia (RLV). Common antigenic determinants of major virus protein of the viruses under study were detected in all groups of patients with haemoblastoses and healthy donors, but the amount of positive results was considerably higher in the group of patients as compared to that in the group of donors. 相似文献