高效液相测定沙棘果皮中熊果酸和齐墩果酸的含量

建立沙棘果皮中齐墩果酸、熊果酸的含量测定方法。采用高效液相色谱法,Nova-Pack型C18柱,乙腈-甲醇-水-醋酸铵（65∶20∶15∶0.5）为流动相,检测波长为210nm;流速0.6mL/min。熊果酸回归方程为y=74.0x＋9.12,r=0.9995,线性范围0.11～10.64μg/mL,齐墩果酸回归方程为y=61.6x＋7.40,r=0.9992,线性范围0.12～12.36μg/mL。沙棘果皮中熊果酸和齐墩果酸的平均含量为1.187mg/g和0.352mg/g,平均回收率为100.3%、100.5%（n=3）,RSD分别为1.47%、1.50%（n=3）。该方法可同时测定沙棘果皮中齐墩果酸和熊果酸的含量,可用于药材质量控制。     

毛细管气相色谱法测定伊班膦酸钠中有机溶剂残留量

建立了毛细管气相色谱法测定伊班膦酸钠中的有机溶剂残留量。采用PEG-20M毛细管色谱柱,30 m×0.32 mm,液膜厚度为1.0μm;柱温60℃,维持5 min后,再以30℃/min的速率升温至180℃,维持2 min;FID检测器;正丙醇为内标进行测定。丙酮、甲醇、乙醇、氯苯的线性范围分别为0.04 957～0.9 913 mg/mL(r=0.999 9)、0.030 56～0.611 3 mg/mL(r=0.999 2)、0.050 68～1.014 mg/mL(r=0.999 8)、0.0 038 29～0.076 58 mg/mL(r=0.999 9);平均回收率分别为101.4%、98.58%、98.42%、99.98%;RSD分别为2.40%、1.86%、2.02%、3.78%(n=9);检测限分别为0.25、0.50、0.25、0.11μg/mL。方法快速、灵敏、准确,适用于伊班膦酸钠中有机溶剂残留量的测定。     

高效液相色谱法分析甲基丙烯酰氧乙基三甲基氯化铵中的阻聚剂

利用反相高效液相色谱法 (HPLC) ,采用ExtendC18柱、以甲醇 -水 ( 0 .0 5 %高氯酸 )为流动相 (体积比为 5 5 :45 ) ,二极管阵列检测器对甲基丙烯酰氧乙基三甲基氯化铵 (简称DMC)中的阻聚剂对羟基苯甲醚进行了检测。试验结果证明 ,对羟基苯甲醚在0 .0 2mg/mL～ 2mg/mL浓度范围内与峰面积呈良好的线性关系。方法回收率为 98.9%～ 10 1.6% ,相对标准偏差为 1.3 2 % ,最低检测限为 5× 10 - 4mg/mL。     

2-苯基-4-对氯苯基-1,5-苯并硫氮杂-α-(丁二酰亚胺基)-β-内酰胺的高效液相色谱法分析

采用Hypersil-ODS2(4.6mm×250mm,5μm)色谱柱,乙腈-水(70∶30)流动相,242nm检测波长,建立了测定2-苯基-4-对氯苯基-1,5-苯并硫氮杂-α-(丁二酰亚胺基)-β-内酰胺的高效液相色谱法。该法线性范为0～0.1mg/mL,回归方程为A=1.63×108c-2.86×105,相关系数r=0.9994,相对标准偏差为0.33%(c=0.06mg/mL,n=5),检出限为0.08μg/mL。平均回收率在97.2%～102.4%之间。该法重现性好,灵敏度高,简单,快速,准确。     

反相高效液相色谱法测定苯扎贝特的含量

研究了采用反相高效液相色谱测定苯扎贝特含量的方法 ,以磷酸盐缓冲液 (0 0 1mol/L的磷酸二氢钾用磷酸调节pH至 3 8) 甲醇 (4∶6 )为流动相 ,采用HypersilC18柱 (5 μm ,4 6mm× 15 0mm) ,检测波长为 2 30nm ,苯扎贝特在 0 0 114～0 114mg/mL范围内线性相关 ,回归方程为 :A =1 0 5× 10 4c(mg/mL) +2 70× 10 2 ,r =0 9997,此法简便、快速、结果准确可靠     

气相色谱测定消心痛中有机溶剂的残留量

建立了C．C测定消心痛原料药中氯仿、乙酸、乙酸乙酯残留量的方法。采用色谱柱为PEG-20M不锈钢柱(4m×2mm),柱温采用程序升高,起始柱温50℃维持3 min,然后以10℃/min的速率升至180℃维持5 min,再以10℃/min的速率升至280℃维持10 min。FID检测器,检测器温度320℃,进样口温度320℃,氮气为载气,流速50mL/min,氢气流速30 mL/min。空气流速400 mL/min。可使三种有机溶剂完全分离,氯仿在0．0005～0．003 mg/mL的浓度范围内呈良好的线性关系(r=0．9994),回收率为100．3％(RSD＜1．0％)；乙酸乙酯在0．005～0．03 mg/mL的浓度范围内呈良好的线性关系(r=0．9999),回收率为100．5％,(RSD＜1．0％)；乙酸在0．005～0．03 mg/mL的浓度范围内呈良好的线性关系(r=0．9997),回收率为98．7％,(RSD＜1．0％)。     

HPLC-MS法测定黄芪中黄酮类成分的含量

采用Acclaim 120 C_(18)色谱柱,以乙腈-0.3%甲酸溶液为流动相,流速为1.0 mL/min,以离子监测(SIM)为扫描模式测定黄芪药材中黄酮类成分含量。研究发现毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素检测的线性范围分别为0.0084～2.217 mg/mL(r=0.9991)、0.0049～1.287 mg/mL(r=0.9995)、0.0027～0.6847 mg/mL(r=0.9994)、0.00194～0.482 mg/mL(r=0.9998);精密度试验、稳定性试验、重复性试验RSD均2.0%;平均加样回收率(n=6)分别为100.31%、99.85%、100.45%、100.72%。该方法简便,可靠,准确,可用于黄芪药材质量控制。     

高效液相色谱法分析复配除草剂中苄嘧磺隆和丁草胺

采用反相C18柱和紫外检测器 ,对丁·苄可湿性粉剂中苄嘧磺隆和丁草胺的测定方法进行了研究。结果表明 ,以甲醇 (A)和醋酸 -醋酸钠缓冲溶液 (B ,pH =5 .6 )为流动相 ,采用梯度淋洗 :0～ 5 .5min ,A∶B =6 5∶35 (v/v) ,5 .6min后 ,改为A∶B =85∶15 (v/v) ;在波长2 5 4nm下检测 ,苄嘧磺隆和丁草胺的变异系数分别为 1.2 0 %和 0 .83% ,平均回收率分别为99.4 %和 99 9% ,苄嘧磺隆和丁草胺的浓度分别在 0 0 0 1～ 0 36 0mg/mL和 0 0 15～7 74 0mg/mL的范围内线性关系良好。方法准确、快速、重现性好 ,已用于丁·苄可湿性粉剂的质量控制。     

2-苯基-4-对氯苯基-1,5-苯并硫氮杂(艹卓)-α-(丁二酰亚胺基)-β-内酰胺的高效液相色谱法分析

采用Hypersil-ODS2(4.6 mm×250mm,5 μm)色谱柱,乙腈-水(70:30)流动相,242 nm检测波长,建立了测定2-苯基-4-对氯苯基-1,5-苯并硫氮杂(艹卓)-α-(丁二酰亚胺基)-β-内酰胺的高效液相色谱法.该法线性范为0～0.1 mg/mL,回归方程为A=1.63×108c-2.86×105,相关系数r=0.9994,相对标准偏差为0.33%(c=0.06 mg/mL,n=5),检出限为0.08 μg/mL.平均回收率在97.2%～102.4%之间.该法重现性好,灵敏度高,简单,快速,准确.     

反相高效液相色谱法测定化妆品中的3-O-乙基维生素

采用反相高效液相色谱法测定化妆品中的3-O-乙基维生素C,色谱条件:SH IMADZU Sh im-pack VP-C18ODS(150 mm×4.6 mm,5μm)色谱柱;C18ODS保护柱芯;流动相V(甲醇)∶V(0.025 mol/L KH2PO4)=20∶80;流速:1.0 mL/m in;柱温:30℃;紫外检测器检测,检测池温度:40℃,波长:254 nm。结果表明,在此条件下,3-O-乙基维生素C在0~100 mg/L与相应的峰面积具有良好的线性关系(r=0.999 2),线性回归方程为A=31.170 3,ρ回收率在97.75%~100.60%,方法精密度RSD约为2.6%(n=5)。     

Many Drugs of Abuse May Be Acutely Transformed to Dopamine,Norepinephrine and Epinephrine In Vivo

It is well established that a wide range of drugs of abuse acutely boost the signaling of the sympathetic nervous system and the hypothalamic–pituitary–adrenal (HPA) axis, where norepinephrine and epinephrine are major output molecules. This stimulatory effect is accompanied by such symptoms as elevated heart rate and blood pressure, more rapid breathing, increased body temperature and sweating, and pupillary dilation, as well as the intoxicating or euphoric subjective properties of the drug. While many drugs of abuse are thought to achieve their intoxicating effects by modulating the monoaminergic neurotransmitter systems (i.e., serotonin, norepinephrine, dopamine) by binding to these receptors or otherwise affecting their synaptic signaling, this paper puts forth the hypothesis that many of these drugs are actually acutely converted to catecholamines (dopamine, norepinephrine, epinephrine) in vivo, in addition to transformation to their known metabolites. In this manner, a range of stimulants, opioids, and psychedelics (as well as alcohol) may partially achieve their intoxicating properties, as well as side effects, due to this putative transformation to catecholamines. If this hypothesis is correct, it would alter our understanding of the basic biosynthetic pathways for generating these important signaling molecules, while also modifying our view of the neural substrates underlying substance abuse and dependence, including psychological stress-induced relapse. Importantly, there is a direct way to test the overarching hypothesis: administer (either centrally or peripherally) stable isotope versions of these drugs to model organisms such as rodents (or even to humans) and then use liquid chromatography-mass spectrometry to determine if the labeled drug is converted to labeled catecholamines in brain, blood plasma, or urine samples.     

Sapindaceae,cyanolipids, and bugs

Scentless plant bugs (Heteroptera: Rhopalidae) are so named because adults of the Serinethinae have vestigial metathoracic scent glands. Serinethines are seed predators of Sapindales, especially Sapindaceae that produce toxic cyanolipids. In two serinethine species whose ranges extend into the southern United States,Jadera haematoloma andJ. sanguinolenta, sequestration of host cyanolipids as glucosides renders these gregarious, aposematic insects unpalatable to a variety of predators. The blood glucoside profile and cyanogenesis ofJadera varies depending on the cyanolipid chemistry of hosts, and adults and larvae fed golden rain tree seeds (Koelreuteria paniculata) excrete the volatile lactone, 4-methyl-2(5H)-furanone, to which they are attracted.Jadera fed balloon vine seeds (Cardiospermum spp.) do not excrete the attractive lactone. Loss of the usual heteropteran defensive glands in serinethines may have coevolved with host specificity on toxic plants, and the orientation ofJadera to a volatile excretory product could be an adaptive response to save time.Mention of a commercial product does not consititute an endorsement by the USDA.     

Insect antifeedant properties of anthranoids from the genusVismia

Vismiones and ferruginins, representatives of a new class of lypophilic anthranoids from the genusVismia were found to inhibit feeding in larvae of species ofSpodoptera, Heliothis, and inLocusta migratoria.     

2008～2009年世界塑料工业进展

收集了2008年7月～2009年6月世界塑料工业的相关资料,介绍了2008～2009年国外塑料工业的发展情况,提供了世界塑料产量、消费量及全球各类树脂的需求量及产能情况。按通用热塑性树脂(聚乙烯、聚丙烯、聚苯乙烯、聚氯乙烯、ABS树脂)、工程塑料(尼龙、聚碳酸酯、聚甲醛、热塑性聚酯、聚苯醚)、特种工程塑料(聚苯硫醚、液晶聚合物、聚醚醚酮)、通用热固性树脂(酚醛、聚氨酯、环氧树脂、不饱和聚酯树脂)不同品种的顺序,对树脂的产量、消费量、供需状况及合成工艺、产品应用开发、树脂品种的延伸及应用的进一步扩展等技术作了详细介绍。     

ICP-MS法测定地球化学样品中As、Cr、Ge、V等18种微量痕量元素的研究

建立了测定地球化学样品中包括As、Cr、Ge、V等18种微量、痕量元素的ICP-MS方法。地化试样用HF-HNO3混酸分解后,以1 1 HNO3溶解干渣。由于制样不使用盐酸,避免了Cl对As、Cr、Ge、V的质谱干扰。用国家一级地球化学标准物质GBW 07309制备溶液优化仪器工作参数,并用于校准。方法测定限(6s)为:0.007~6.4μg/g,精密度(RSD%,n=12)为:29%~9.4%,经过国家一级地球化学标准物质的分析验证,结果与标准值吻合。方法已应用于国土资源调查的试样分析。