Resilience and restoration of tropical and subtropical grasslands,savannas, and grassy woodlands

Despite growing recognition of the conservation values of grassy biomes, our understanding of how to maintain and restore biodiverse tropical grasslands (including savannas and open‐canopy grassy woodlands) remains limited. To incorporate grasslands into large‐scale restoration efforts, we synthesised existing ecological knowledge of tropical grassland resilience and approaches to plant community restoration. Tropical grassland plant communities are resilient to, and often dependent on, the endogenous disturbances with which they evolved – frequent fires and native megafaunal herbivory. In stark contrast, tropical grasslands are extremely vulnerable to human‐caused exogenous disturbances, particularly those that alter soils and destroy belowground biomass (e.g. tillage agriculture, surface mining); tropical grassland restoration after severe soil disturbances is expensive and rarely achieves management targets. Where grasslands have been degraded by altered disturbance regimes (e.g. fire exclusion), exotic plant invasions, or afforestation, restoration efforts can recreate vegetation structure (i.e. historical tree density and herbaceous ground cover), but species‐diverse plant communities, including endemic species, are slow to recover. Complicating plant‐community restoration efforts, many tropical grassland species, particularly those that invest in underground storage organs, are difficult to propagate and re‐establish. To guide restoration decisions, we draw on the old‐growth grassland concept, the novel ecosystem concept, and theory regarding tree cover along resource gradients in savannas to propose a conceptual framework that classifies tropical grasslands into three broad ecosystem states. These states are: (1) old‐growth grasslands (i.e. ancient, biodiverse grassy ecosystems), where management should focus on the maintenance of disturbance regimes; (2) hybrid grasslands, where restoration should emphasise a return towards the old‐growth state; and (3) novel ecosystems, where the magnitude of environmental change (i.e. a shift to an alternative ecosystem state) or the socioecological context preclude a return to historical conditions.     

Vegetation in clear‐cuts depends on previous land use: a century‐old grassland legacy

Plant species richness in central and northern European seminatural grasslands is often more closely linked to past than present habitat configuration, which is indicative of an extinction debt. In this study, we investigate whether signs of historical grassland management can be found in clear‐cuts after at least 80 years as coniferous production forest by comparing floras between clear‐cuts with a history as meadow and as forest in the 1870s in Sweden. Study sites were selected using old land‐use maps and data on present‐day clear‐cuts. Species traits reflecting high capacities for dispersal and persistence were used to explain any possible links between the plants and the historical land use. Clear‐cuts that were formerly meadow had, on average, 36% higher species richness and 35% higher richness of grassland indicator species, as well as a larger overall seed mass and lower anemochory, compared to clear‐cuts with history as forest. We suggest that the plants in former meadows never disappeared after afforestation but survived as remnant populations. Many contemporary forests in Sweden were managed as grasslands in the 1800s. As conservation of remaining grassland fragments will not be enough to reduce the existing extinction debts of the flora, these young forests offer opportunities for grassland restoration at large scales. Our study supports the concept of remnant populations and highlights the importance of considering historical land use for understanding the distribution of grassland plant species in fragmented landscapes, as well as for policy‐making and conservation.     

Dominant Grasses Suppress Local Diversity in Restored Tallgrass Prairie

Warm‐season (C₄) grasses commonly dominate tallgrass prairie restorations, often at the expense of subordinate grasses and forbs that contribute most to diversity in this ecosystem. To assess whether the cover and abundance of dominant grass species constrain plant diversity, we removed 0, 50, or 100% of tillers of two dominant species (Andropogon gerardii or Panicum virgatum) in a 7‐year‐old prairie restoration. Removing 100% of the most abundant species, A. gerardii, significantly increased light availability, forb productivity, forb cover, species richness, species evenness, and species diversity. Removal of a less abundant but very common species, P. virgatum, did not significantly affect resource availability or the local plant community. We observed no effect of removal treatments on critical belowground resources, including inorganic soil N or soil moisture. Species richness was inversely correlated with total grass productivity and percent grass cover and positively correlated with light availability at the soil surface. These relationships suggest that differential species richness among removal treatments resulted from treatment induced differences in aboveground resources rather than the belowground resources. Selective removal of the dominant species A. gerardii provided an opportunity for seeded forb species to become established leading to an increase in species richness and diversity. Therefore, management practices that target reductions in cover or biomass of the dominant species may enhance diversity in established and grass‐dominated mesic grassland restorations.     

Multi‐scale responses of plant species diversity in semi‐natural buffer strips to agricultural landscapes

Question: How does agricultural land usage affect plant species diversity in semi‐natural buffer strips at multiple scales? Location: Lepsämä River watershed, Nurmijärvi, Southern Finland. Methods: Species diversity indicators included both richness and evenness. Plant communities in buffer strips were surveyed in 29 sampling sites. Using ArcGIS Desktop 9.0 (ArcInfo) and Fragstats 3.3 for GIS analysis, the landscape composition around each sampling site was characterized by seven parameters in square sectors at five scales: 4, 36, 100, 196, and 324ha. For each scale, Principle Component Analysis was used to examine the importance of each structural metric to diversity indicators using multiple regression and other simple analyses. Results: For all but the smallest scales (4 ha), two structural metrics including the diversity of land cover types and percentage of arable land were positively and negatively correlated with species richness, respectively. Both metrics had the highest correlation coefficients for species richness at the second largest scale (196 ha). The density of arable field edges between the fields was the only metric that correlated with species evenness for all scales, which had highest predictive power at the second smallest scale (36 ha). Conclusions: Species richness and evenness of buffer strips had scale‐dependent relationships to land use in agricultural ecosystems. The results of this study indicated that species richness depends on the pattern of arable land use at large scales, which may relate to the regional species pool. Meanwhile, species evenness depended on the level of field edge density at small scales, which relates to how the nearby farmland was divided by the edges (e.g. many small‐scale fields with high edge density or a few big‐scale fields with low edge density). This implies that it is important to manage the biodiversity of buffer strips within a landscape context at multiple scales.     

Effects of Species Richness on Resident and Target Species Components in a Prairie Restoration

The ecological role of biodiversity in achieving successful restoration has been little explored in restoration ecology. We tested the prediction that we are more likely to create persistent, species‐rich plant communities by increasing the number of species sown, and, to some degree, by varying functional group representation, in experimental prairie plantings. There were 12 treatments consisting of 1‐, 2‐, 3‐, 4‐, 8‐, 12‐, and 16‐species mixtures of native perennials representing four functional groups (C₄ grasses, C₃ grasses, nitrogen‐fixing species, and late‐flowering composites) that predominate within Central Plains tallgrass prairies. In 2000, species were seeded into square plots (6 × 6 m), with five replicates per treatment, on former agricultural land. Annually, we measured total species richness and evenness, target species richness and cover, and richness and cover of resident species (i.e., those emerging from the seed bank). Both target species richness and rate of establishment of target communities were highest in the most species‐rich mixtures, but there was no additional benefit for treatments that contained more than eight species. Richness of resident species did not vary with target species richness; however, cover by resident species was lower in the higher target species treatments. Our results, indicating that establishment of species‐rich prairie mimics can be enhanced by starting with larger numbers of species at the outset, have implications for grassland restoration in which community biodiversity creation and maintenance are key goals.     

Invasive species cover,soil type,and grazing interact to predict long‐term grassland restoration success

Grasslands are undergoing tremendous degradation as a result of climate change, land use, and invasion by non‐native plants. However, understanding of the factors responsible for driving reestablishment of grassland plant communities is largely derived from short‐term studies. In order to develop an understanding of the factors responsible for longer term restoration outcomes in California annual grasslands, we surveyed 12 fields in Davis, CA, U.S.A., in 2015 that were seeded with native species mixtures starting in 2004. Using field surveys, we investigated how invasive plant richness and cover, native plant richness and cover, aboveground biomass, grazing, soil type, and restoration species identity might provide utility for explaining patterns of restoration success. We found a negative relationship between invasive cover and restoration cover, which was attributed to the slow establishment of seeded species and subsequent dominance by weeds. The relationship between invasive cover and restoration cover was modified by grazing, likely due to a change in the dominance of exotic forbs, which have a more similar growing season to restoration species, and therefore compete more strongly for late season moisture. Finally, we found that soil type was responsible for differences in the identity and abundance of invasive plants, subsequently affecting restoration cover. This work highlights the value of focusing resources on reducing invasive species cover, limiting grazing to periods of adequate moisture, and considering soil type for successful long‐term restoration in California annual grasslands. Moreover, observations of long‐term restoration outcomes can provide insight into the way mechanisms driving restoration outcomes might differ through time.     

Effects of water and nitrogen addition on species turnover in temperate grasslands in northern China

Global nitrogen (N) deposition and climate change have been identified as two of the most important causes of current plant diversity loss. However, temporal patterns of species turnover underlying diversity changes in response to changing precipitation regimes and atmospheric N deposition have received inadequate attention. We carried out a manipulation experiment in a steppe and an old-field in North China from 2005 to 2009, to test the hypothesis that water addition enhances plant species richness through increase in the rate of species gain and decrease in the rate of species loss, while N addition has opposite effects on species changes. Our results showed that water addition increased the rate of species gain in both the steppe and the old field but decreased the rates of species loss and turnover in the old field. In contrast, N addition increased the rates of species loss and turnover in the steppe but decreased the rate of species gain in the old field. The rate of species change was greater in the old field than in the steppe. Water interacted with N to affect species richness and species turnover, indicating that the impacts of N on semi-arid grasslands were largely mediated by water availability. The temporal stability of communities was negatively correlated with rates of species loss and turnover, suggesting that water addition might enhance, but N addition would reduce the compositional stability of grasslands. Experimental results support our initial hypothesis and demonstrate that water and N availabilities differed in the effects on rate of species change in the temperate grasslands, and these effects also depend on grassland types and/or land-use history. Species gain and loss together contribute to the dynamic change of species richness in semi-arid grasslands under future climate change.     

中国东北样带草地群落放牧干扰植物多样性的变化

放牧干扰是草地群落植物多样性变化的主要影响因素之一。中国东北样带9个草地群落放牧干扰植物多样性变化的研究结果表明：中牧或重牧阶段Shannon指数达最大值,形成中牧（重牧）>重牧（中牧）>轻牧>过牧的规律。群落物种丰富度、均匀度与多样性的相关分析表明,均匀度变化对多样性变化具有更大的贡献率,而丰富度呈下降趋势,即轻牧（中牧）>中牧（轻牧）>重牧>过牧。生活型功能群多样性也表现出明显的变化。中国东北样带草地群落植物多样性的分布格局是：草甸草原>典型草原>典型草原 >荒漠草原>碱化草原,并且群落物种丰富度对多样性有更大贡献率。     

Weak functional response to agricultural landscape homogenisation among plants,butterflies and birds

Measures of functional diversity are expected to predict community responses to land use and environmental change because, in contrast to taxonomic diversity, it is based on species traits rather than their identity. Here, we investigated the impact of landscape homogenisation on plants, butterflies and birds in terms of the proportion of arable field cover in southern Finland at local (0.25 km²) and regional (> 10 000 km²) scales using four functional diversity indices: functional richness, functional evenness, functional divergence and functional dispersion. No uniform response in functional diversity across taxa or scales was found. However, in all cases where we found a relationship between increasing arable field cover and any index of functional diversity, this relationship was negative. Butterfly functional richness decreased with increasing arable field cover, as did butterfly and bird functional evenness. For butterfly functional evenness, this was only evident in the most homogeneous regions. Butterfly and bird functional dispersion decreased in homogeneous regions regardless of the proportion of arable field cover locally. No effect of landscape heterogeneity on plant functional diversity was found at any spatial scale, but plant species richness decreased locally with increasing arable field cover. Overall, species richness responded more consistently to landscape homogenisation than did the functional diversity indices, with both positive and negative effects across species groups. Functional diversity indices are in theory valuable instruments for assessing effects of land use scenarios on ecosystem functioning. However, the applicability of empirical data requires deeper understanding of which traits reliably capture species’ vulnerability to environmental factors and of the ecological interpretation of the functional diversity indices. Our study provides novel insights into how the functional diversity of communities changes in response to agriculturally derived landscape homogenisation; however, the low explanatory power of the functional diversity indices hampers the ability to reliably anticipate impacts on ecosystem functioning.     

中国东北样带草地群落放牧干扰植物多样的变化

放牧干扰是草地群落植物多样性变化的主要影响因素之一。中国东北样带９个草地群落放牧干扰植物多样性性变化的研究结果表明：中牧或重牧阶段Ｓhannon指数达最大值。形成中牧（重牧）＞重牧（中牧）＞轻牧＞过牧的规律。群落物种丰富度、均匀度与多样性的相关分析表明,均匀度变化对多样性变化具有更大的贡献率。而丰富度呈下降趋势,即轻牧（中牧）＞中牧（轻牧）＞重牧＞过牧。生活型功能群多样性也表现出明显的变化。中国东北样带草地群落植物多样性的分布格局是：草甸草原＞典型草原＞典型草原＞荒漠草原＞碱化草甸,并且群落物种丰富度对多样性有更大贡献率。     

Top predator control of plant biodiversity and productivity in an old-field ecosystem

Abstract Predators can have strong indirect effects on plants by altering the way herbivores impact plants. Yet, many current evaluations of plant species diversity and ecosystem function ignore the effects of predators and focus directly on the plant trophic level. This report presents results of a 3‐year field experiment in a temperate old‐field ecosystem that excluded either predators, or predators and herbivores and evaluated the consequence of those manipulations on plant species diversity (richness and evenness) and plant productivity. Sustained predator and predator and herbivore exclusion resulted in lower plant species evenness and higher plant biomass production than control field plots representing the intact natural ecosystem. Predators had this diversity‐enhancing effect on plants by causing herbivores to suppress the abundance of a competitively dominant plant species that offered herbivores a refuge from predation risk.     

Canopy thinning,not agricultural history,determines early responses of wild bees to longleaf pine savanna restoration

Longleaf pine savannas are highly threatened, fire‐maintained ecosystems unique to the southeastern United States. Fire suppression and conversion to agriculture have strongly affected this ecosystem, altering overstory canopies, understory plant communities, and animal populations. Tree thinning to reinstate open canopies can benefit understory plant diversity, but effects on animal communities are less well understood. Moreover, agricultural land‐use legacies can have long‐lasting impacts on plant communities, but their effects on animal communities either alone or through interactions with restoration are unclear. Resolving these impacts is important due to the conservation potential of fire‐suppressed and post‐agricultural longleaf savannas. We evaluated how historical agricultural land use and canopy thinning affect the diversity and abundance of wild bees in longleaf pine savannas. We employed a replicated, large‐scale factorial block experiment in South Carolina, where canopy thinning was applied to longleaf pine savannas that were either post‐agricultural or remnant (no agricultural history). Bees were sampled using elevated bee bowls. In the second growing season after restoration, thinned plots supported a greater bee abundance and bee community richness. Additionally, restored plots had altered wild bee community composition when compared to unthinned plots, indicating that reduction of canopy cover by the thinning treatment best predicted wild bee diversity and composition. Conversely, we found little evidence for differences between sites with or without historical agricultural land use. Some abundant Lasioglossum species were the most sensitive to habitat changes. Our results highlight how restoration practices that reduce canopy cover in fire‐suppressed savannas can have rapid benefits for wild bee communities.     

Influence of soil properties on coastal sandplain grassland establishment on former agricultural fields

The decline in species‐rich grasslands across the United States has increased the importance of conservation and restoration efforts to preserve the biodiversity supported by these habitats. Abandoned agricultural fields often provide practical locations for the reestablishment of species‐rich grasslands. However, these fields often retain legacies of agriculture both in their soils, which may have higher pH and nitrogen (N) contents than soils that were never farmed, and in their plant communities, which are dominated by non‐native species and poor in native seed stock. We considered methods of reversing these legacies to create native‐species‐rich grassland on former agricultural land. We tested seeding and tilling combined with additions of sulfur (S), carbon (C), N or water to establish diverse sandplain grassland vegetation on an old field on Martha's Vineyard, Massachusetts. We measured soil pH, extractable nitrate and ammonium, and total and native species richness and native species cover for 5 years after treatment. S additions lowered pH to values typical of never‐tilled sandplain ecosystems and increased native species cover, but had no effect on species richness. C, N, and water additions had no significant effects on the soil or vegetation. Seeding and tilling were more effective at restoring native species richness than any soil amendments and indicated a greater importance of biotic factors compared with soil conditions in promoting sandplain vegetation establishment. S amendment accelerated establishment of native species cover for several years but the effect of S additions compared with seeding and tilling alone declined over time.     

Old‐field grassland successional dynamics following cessation of chronic disturbance

Question: Does increasing Festuca canopy cover reduce plant species richness and, therefore, alter plant community composition and the relationship of litter to species richness in old‐field grassland? Location: Southeastern Oklahoma, USA. Methods: Canopy cover by species, species richness, and litter mass were collected within an old‐field grassland site on 16, 40 m × 40 m plots. Our study was conducted during the first three years of a long‐term study that investigated the effects of low‐level nitrogen enrichment and small mammal herbivory manipulations. Results: Succession was altered by an increase in abundance of Festuca over the 3‐yr study period. Species richness did not decline with litter accumulation. Instead, Festuca increased most on species‐poor plots, and Festuca abundance remained low on species‐rich plots. Conclusions: Festuca may act as an invasive transformer‐species in warm‐season dominated old‐field grasslands, a phenomenon associated more with invasions of cool‐season grasses at higher latitudes in North America.     

Phenological Complementarity Does not Enhance Ecosystem Production in Undisturbed Steppe Community

Communities with more species could have a greater variety of species＇ characteristics, leading to more effective use of limiting resources through niche partitioning （complementarity） and therefore greater production. The effect of phenologlcal complementarity （PC） on ecosystem production has not been fully Investigated. The seasonal responses of all vascular plant species were tracked to test the effect of phenologlcal complementarity on ecosystem production within a natural stable steppe community. Although a significant phenologlcal pattern was observed, PC had no significant correlation with community production. The value of PC varied with years, but was observed only In a relatively narrow range during the experimental period. Species diversity （richness and evenness） had no correlation with the ecosystem production. The results suggest that the effect of PC may be saturated and has no contribution to the improvement of ecosystem production In a stable natural grassland community with abundant species.     

Vegetation removal and seed addition contribute to coastal sandplain grassland establishment on former agricultural fields

Creating native‐species‐rich grasslands to replace agricultural grasslands can be an important strategy for supplementing the area of grasslands, which are in decline in many regions. In the northeastern United States, sandplain grasslands support a diverse plant community and rare plant and animal species that are declining because of reductions in historical disturbances such as fire and grazing. We designed an experiment on Martha's Vineyard, Massachusetts, to test methods of establishing native‐species‐rich coastal sandplain grassland on former agricultural land. We tested the efficacy of: (1) tilling, herbicide, hot foam, and plastic cover in removing initial nonnative vegetation, and (2) combinations of tilling and seeding for establishing native species. We measured native and nonnative species richness and percent cover before and for 5 years after treatment. Herbicide, plastic cover, and spring, summer, and fall tilling were about equally effective in reducing nonnative species cover and promoting native species cover. Tilling and seeding each increased native species richness and percent cover, and seeding and tilling together increased native species richness and cover more than either treatment alone. Combined seeding and disturbance also reduced the cover of nonnative species, but nonnative species cover remained higher than in adjacent reference sandplain grassland. Results indicated that native species establishment was enhanced by the availability of seeds and by reduction of initial nonnative plant cover. The most efficient method of converting coastal agricultural grasslands to sandplain grassland with a higher number and proportion of native species is a single season of plant removal and seeding.     

Land‐use history,historical connectivity,and land management interact to determine longleaf pine woodland understory richness and composition

Restoration and management activities targeted at recovering biodiversity can lead to unexpected results. In part, this is due to a lack of understanding of how site‐level characteristics, landscape factors, and land‐use history interact with restoration and management practices to determine patterns of diversity. For plants, such factors may be particularly important since plant populations often exhibit lagged responses to habitat loss and degradation. Here, we assess the importance of site‐level, landscape, and historical effects for understory plant species richness and composition across a set of 40 longleaf pine Pinus palustris woodlands undergoing restoration for the federally endangered red‐cockaded woodpecker in the southeastern United States. Land‐use history had an overarching effect on richness and composition. Relative to historically forested sites, sites with agricultural histories (i.e. former pastures or cultivated fields) supported lower species richness and an altered species composition due to fewer upland longleaf pine woodland community members. Landscape effects did not influence the total number of species in either historically forested or post‐agricultural sites; however, understory species composition was affected by historical connectivity, but only for post‐agricultural sites. The influences of management and restoration activities were only apparent once land‐use history was accounted for. Prescribed burning and mechanical overstory thinning were key drivers of understory composition and promoted understory richness in post‐agricultural sites. In historically forested sites these activities had no impact on richness and only prescribed fire influenced composition. Our findings reveal complex interplays between site‐level, landscape, and historical effects, suggest fundamentally different controls over plant communities in longleaf pine woodlands with varying land‐use history, and underscore the importance of considering land‐use history and landscape effects during restoration.     

高寒草地植物物种多样性与功能多样性的关系

物种多样性与功能多样性的关系是生态学当前研究的热点问题之一,不同区域典型生态系统物种多样性和功能多样性的关系研究有利于生物多样性保护理论的全面发展。以青藏高原地区的主要草地生态系统—高寒草甸和高寒草原为研究对象,采用4个物种多样性指数(Patrick丰富度指数、Shannon-Weiner多样性指数、Pielou均匀度指数和Simpson优势度指数)和9个功能多样性指数(FAD功能性状距离指数、MFAD功能性状平均距离指数、基于样地的FDp和基于群落的FDc功能树状图指数、FRic功能体积指数、FEve功能均匀度指数、Rao功能离散度常二次熵指数、FDiv功能离散指数、FDis功能分散指数),分析了高寒草地植物物种多样性、功能多样性关系及其与初级生产力的关系,以期阐明3个科学问题:不同草地类型的高寒草地生态系统植物物种多样性和功能多样性有何差异?高寒草地生态系统的植物物种多样性和功能多样性有何关系?高寒草地生态系统物种多样性、功能多样性对生态系统功能的影响有何异同?研究结果表明:(1)与高寒草原相比,高寒草甸具有更高的物种多样性、功能丰富度和功能离散度;(2)高寒草甸中,Patrick丰富度与功能丰富度指数(FAD、MFAD、FDp、FDc)和功能离散度指数(FDiv)的具有较强的相关性,最优拟合方程分别为幂函数和二次多项式函数;(3)高寒草原中,Patrick丰富度与功能丰富度指数(FAD、MFAD、FDp、FDc、FRic)、Shannon指数和Simpson指数与FEve指数的相关性较强,最优拟合方程为二次多项式函数,Pielou指数与FEve指数的相关性较强,最优拟合方程为指数函数;(4)高寒草甸的初级生产力分别与物种丰富度指数Patrick、功能离散指数FDiv具有较强的相关性;而高寒草原的初级生产力与4个物种多样性指数间均具有较强的相关性,与功能离散指数FDiv具有较强的相关性,最佳拟合方程均为二次多项式函数。研究的总体结论为:物种多样性、功能多样性、二者之间的关系以及二者与生态系统服务功能(以初级生产力为例)之间的关系在高寒草甸和高寒草原群落中表现迥异,因此在研究青藏高原高寒草地的生态功能时,不能仅仅测度传统的物种多样性,还应测度与物种多样性、生态功能密切相关的功能多样性。     

Problems, Approaches, and Results in Restoration of Dutch Calcareous Grassland During the Last 30 Years

This paper is based on research of the restoration of species‐rich calcareous grasslands in The Netherlands, over the last 30 years. Chalk grassland is a semi‐natural vegetation with a high density of species at a small scale. This type of vegetation was once widespread in Western Europe as common grazing land, mainly for flocks of sheep for which the main function was dung production. In some regions of Central Europe, these grasslands were also used for hay production. The dung was used to maintain arable field production at a reasonable level. In the chalk district in the southernmost part of The Netherlands some 25 sites of this vegetation, varying in area from 0.05–4.5 ha, are still present. Chalk grassland completely lost its significance for modern agricultural production after the wide application of artificial fertilizer following World War II. This grassland has a high conservation value both for plants and animal species, of which a large number of species are exclusively restricted to this biotope. When conservation activities started at a large scale in the early 1960s, three different types of restoration activities could be distinguished: (1) restoration of fertilized sites; (2) restoration of abandoned grasslands; and (3) recreation of chalk grassland on former arable fields. The main aim of the restoration attempt is to create and/or improve sustainable conditions for both plant and animal species characteristic of the chalk grassland ecosystem. In the process of restoration, several phases of different activities can be distinguished: (1) pre‐restoration phase, during which information of the land use history is collected and, based on these data, clear restoration goals are established; (2) initial restoration phase, during which effects of former, non‐conservational land use has to be undone in order to stimulate germination and establishment of target species originating from soil seed bank and species pool; (3) consolidation phase, including the introduction and continuation of a regular management system for sustainable conservation; and (4) long‐term conservation strategy, including measures to prevent disturbance from the outside and genetic erosion and extinction of locally endangered plant populations.