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介绍一种利用细胞表面疏水性原理快速而简单测定酵母细胞絮凝性的方法(HICF法)。酵母细胞悬浮液注入Sepharose凝胶柱 ,然后加入含有氯化钠的缓冲液 ,疏水细胞吸附于凝胶柱 ,通过测定吸附于凝胶柱的细胞的百分数可反映出酵母细胞的疏水程度[1],以此指示酵母的絮凝性。 相似文献
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研究拮抗酵母结合热空气处理对提高采后海红果果实贮藏性能的效果。分别用107CFU/mL拮抗罗伦隐球酵母悬浮液浸泡处理2 min,36℃热空气处理海红果10 h以及二者结合先后处理海红果,于20℃下储藏15 d,贮藏期每3 d测定相关指标,研究不同处理对海红果果实贮藏期的保鲜效果。结果表明,热空气处理、拮抗酵母处理、先拮抗酵母后热空气处理和先热空气后拮抗酵母处理均能降低贮藏过程中海红果的失重率、腐烂率和丙二醛含量,提高SOD、CAT和POD活性,其中先热空气后拮抗酵母处理组的各项指标除POD外,其他均显著(P<0.05)优于单一处理组,表明先热空气后拮抗酵母处理海红果的保鲜效果优于单独处理方式;能明显降低果实水分和有机质的减少以及病害的发生率,提高果实抗氧化酶活性,减少丙二醛的积累,进而延缓细胞衰老,延长货架期。先36℃热空气处理10 h后107 CFU/mL罗伦隐球酵母悬浮液浸泡处理2 min是采后海红果果实保鲜处理的适宜方式。 相似文献
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文章是专门针对目前国内外市场上对富含I+G的抽提物的需求越来越大而专门研制的高核酸型啤酒酵母抽提物。采用分步酶解啤酒酵母制取高核酸酵母抽提物,得出了较优的酶解工艺条件。抽提酶酶解最适条件:酵母底物浓度10%,0.04%酵母悬浮液量的外加抽提酶量,22h的酶解时间,55℃酶解,pH值为6.5。核酸酶酶解最适条件:酵母底物浓度10%,0.02%酵母悬浮液量的外加核酸酶量,6h的酶解时间,70℃酶解,pH值为5.0。最后,酵母抽提物的呈味核苷酸(I+G)含量由原来的3%~4%提高到10%~12%。 相似文献
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本文介绍了一种评价酵母性状的方法--酵母肝糖含量的检测,将此项目引入酵母扩培、酵母筛选、酵母回收等工作后可更好的对酵母使用进行管理. 相似文献
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啤酒废酵母自溶条件的研究 总被引:5,自引:0,他引:5
探讨了以啤酒废酵母为原料,采用自溶法生产酵母抽提物的条件。结果表明:以食盐为啤酒酵母自溶促进剂的最佳作用条件是10%的啤酒酵母悬浮液在pH5·0、温度50℃、食盐浓度3%下,自溶40h,所得酵母抽提液的氨基氮含量为0·498g/100mL,产品得率47·50%,风味非常醇厚。 相似文献
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为提高质量检验和工艺研究结论的科学性和可靠性,以具体的质量检验和工艺试验研究为例,探讨了非参数检验中符号检验法和秩和检验法的应用方法及注意事项。在非参数检验中,符号检验法不需考虑总体的理论分布,均可准确地计算概率,且方法简单、适用性广,不足之处是只考虑差数为正或负的个数,而不考虑差数的绝对值大小,是一种比较粗放的检验方法;而秩和检验法则能从一定程度上克服符号检验法的不足,具有较高的灵敏度。 相似文献
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家具整体测试法是家具毒害物质测试的趋势。本文主要对家具测试用环境气候舱的产生背景、原理构造、测试优点等方面进行简要介绍。 相似文献
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The effectiveness of various assay systems for the examination of trout was investigated. The Four Plate test (FPT), Premi
test and ELISA test were used for determining sulfadiazine/trimethoprim residues in trout. According to the results of the
Four Plate test, when trouts were fed with pellets containing antibiotics (sulfadiazine/trimethoprim), the inhibition zones
increased according to duration of feed application. In the control group, no inhibition zones were detected. The concentration
of the residues accumulated and reached a plateau after 5 days. The antibiotics were detectable in the same concentration
as on day 10, but 5 days later (on day 15) they were no longer detectable in the trout by EEC FPT. In contrast to the FPT,
5 days after discontinuing the medicated diet, there were still residues detectable by the Premi test and ELISA test on day 15.
According to the ELISA test results of the trout; from day 1 to day 10 the results of sulfadiazine residues were found to
be 4.18, 8.02, 9.13, 20.10, 21.32, 20.52, 20.75, 20.99, 20.90, 20.32 ppb, respectively. On day 10, the antibiotics were detectable
in the same concentration. On day 15, sulfadiazine residues were found to be 6.82 ppb, but 5 days later (on day 20) they were
no longer detectable in the trout. EEC Four Plate method presented less sensitivity and effectiveness in the detection of
sulfadiazine. 相似文献
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Vasconcelos J Martins A Petim-Batista MF Colaço J Blake RW Carvalheira J 《Journal of dairy science》2004,87(8):2591-2598
We evaluated the accuracy of an autoregressive multiple-lactation test day (ATD) model to predict missing test day yields of milk, fat, and protein to obtain cumulative 305-d records for cows with incomplete or in-progress lactations. The data consisted of more than one million observations of daily yields on test days in the first 3 lactations of over 75,000 Portuguese Holstein cows. Differences between actual (estimates from complete lactations using the test interval method) and ATD-predicted 305-d yields were negligible and smaller than those predicted by the test interval method. The ATD procedure tended to slightly underestimate cumulative lactation yields, whereas the test interval method substantially overestimated them. Smaller differences obtained by the ATD procedure resulted in less biased estimates of lactation yield, which also implies greater accuracy. As expected, the correlations between actual and predicted lactation yields increased with the number of test days from 0.831 to 0.997. Average correlations (by parity) between actual and ATD-predicted yields ranged from 0.977 to 0.984. Correlations between actual test day yields and corresponding predicted yields exceeded 0.5 for up to 7 time-intervals from the last test day yield used to predict cumulative yield of projected lactations. These correlations indicate the good predictive ability of the ATD method. From a producer's viewpoint, these advantages underwrite management because most on-farm selection decisions are based on the producing abilities of cows. Implementation of ATD methodology does not require special computing capability and is easily transferable to the farm level. 相似文献
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目的 调查郑州市部分食品中二氧化硫残留情况, 比对快速检测法与国标法检测二氧化硫的测定结果。方法 在郑州市随机选取大型的农贸市场及超市采集食用菌、坚果、干制蔬菜等6大类食品共计192份, 采用快检法与国标法对其中的二氧化硫进行检测, 并对2种方法的检测结果进行比对。结果 192份样品中, 国标法检出176份含有二氧化硫, 检出率为92%, 超标123份, 超标率为64%。而快检法只有部分高残留食品(枸杞、干制蔬菜及食用菌)的检测结果与国标法较为相近, 对调料及水果干检测结果不理想。结论 郑州市售部分调料、枸杞、干制蔬菜及食用菌中二氧化硫污染严重, 有关部门应加强日常监管工作。此外二氧化硫速测试剂盒并不是对所有的食品都有效, 在日常大量抽检工作的使用中应注意适用范围。 相似文献