大白菜相对于结球甘蓝连锁群特异SSR标记的筛选

以9个大白菜品种和10个结球甘蓝品种为试材,对位于芸薹属A基因组10个连锁群的207对SSR引物进行PCR筛选,共筛选出33个大白菜相对于结球甘蓝的特异SSR标记,涉及了大白菜的10个连锁群,其中位于A1连锁群3对,A2连锁群4对,A3连锁群5对,A4连锁群2对,A5连锁群3对,A6连锁群4对,A7连锁群2对,A8连锁群1对,A9连锁群2对,A10连锁群7对。为进一步利用SSR标记鉴定结球甘蓝—大白菜异附加系奠定了基础。     

Chromosomal assignments of 23 biochemical loci of the rat by using rat x mouse somatic cell hybrids

A panel of 18 rat x mouse somatic cell hybrid clones segregating individual rat chromosomes in different combinations was used to assign 23 biochemical loci to rat chromosomes. The chromosomal locations for these 23 loci were determined as follows: GOT1 on rat chromosome 1; HAGH on 2; ACP2, ADA, GANC, ITPA, and SORD on 3; LDHB on 4; PEPB on 7; GLB1 and HEXA on 8; IDH1 on 9; UMPH2 on 10; GUSB on 12; FH and PEPC on 13; PEPS on 14; ESD and NP on 15; DIA4 on 19; and PP on 20. In addition, ACP1 and GLO1 were reassigned to rat chromosomes 6 and 20, respectively. The chromosomal assignments of these loci extends the known syntenic homologies among rats, mice, and humans.     

New species of Laboulbenia from Ecuador, with evidence for host switch in the Laboulbeniales

Ten new species of Laboulbenia from Ecuador are described. These are L. barraganii, parasitic on Platamops sp. (Salpingidae); L. biformis, parasitic on Diploharpus rossii Moret (Carabidae); L. davidsonii, parasitic on Odontocheila spp. (Carabidae); L. gregaria, parasitic on Philonthus sp. (Staphylinidae); L. micrandra, parasitic on Lobrathium sp. (Staphylinidae); L. mycotreti, parasitic on Mycotretus spp. (Erotylidae); L. opima, parasitic on Chrysodinopsis sp. (Chrysomelidae); L. otongaensis, parasitic on Diploharpus iridescens Moret (Carabidae); L. tapiae, parasitic on Priocera sp. (Cleridae); and L. trogacti, parasitic on Trogactus sp. (Staphylinidae). Laboulbenia barraganii is the first of the Laboulbeniales to be reported on beetles of the family Salpingidae. L. biformis displays a moderate dimorphism associated with the position on the host insect. L. davidsonii, which is very different from the other species of Laboulbenia reported so far on the Cicindelinae but similar to a group of species parasitic on Galerita spp., provides evidence for the occurrence of host switch in Laboulbeniales.     

不同寄主植物对云南紫胶虫自然种群的影响

研究了云南紫胶虫(Kerria yunnanensis)在聚果榕、南岭黄檀、钝叶黄檀和偏叶榕上的发育、存活和繁殖情况.结果表明：在自然条件下,云南紫胶虫在钝叶黄檀上的种群初始密度最高,为181.17头·cm^-2,聚果榕上最低,为145.27头·cm^-2;雄虫占的比例以在南岭黄檀上最少,为0.19,偏叶榕和聚果榕上最多,为0.24;从幼虫到成虫的累积死亡率,南岭黄檀上最高,为90.03%,偏叶榕上最低,为87.05%;完成世代时间以在南岭黄檀上最长,为156 d,聚果榕上最短,为140 d;平均怀卵量以在南岭黄檀上最高,为536粒,聚果榕上最低,为345.86粒;虫体质量以在南岭黄檀上最大,为12.62 mg,偏叶榕上最小,为9.03 mg;世代净增值率以在南岭黄檀上最高,为48.51,聚果榕上最小,为32.79.综合比较4种不同寄主植物,南岭黄檀是云南紫胶虫夏季世代种群生长发育和繁殖最适宜的寄主.     

The effects of improvement of upland, marginal grasslands on the breeding success of Lapwings Vanellus vanellus and other waders

Data on the breeding success of Lapwings Vanellus vanellus on unimproved and improved grassland, with comparative data for arable land, were obtained from a study on 760 Lapwing clutches. The study was undertaken in the Eden Valley, Cumbria, and Teesdale, Co. Durham, between 1985 and 1987. First clutches were larger on unimproved grassland with a mean of 3.73 eggs compared to 3.61 eggs on improved grassland. In contrast, replacement clutches were larger on improved areas (3.90 eggs) than on unimproved (3.47 eggs). On average, 40% of eggs laid on unimproved pastures hatched compared to only 17% on improved pastures. No significant difference in hatching success was found between unimproved and improved meadows with 32% and 22% of eggs hatching, respectively. Overall, 73% of unsuccessful first clutches were replaced on unimproved pastures, whereas on meadows and improved pastures combined, only 32% were replaced. Survival of small chicks was highest on unimproved areas. Production on unimproved areas was sufficient to replace adult losses and so maintain numbers, whereas on improved land production was too low to maintain existing breeding densities. Hatching success on unimproved areas was similar for the four species of wader considered. On improved areas, Redshank hatching success was relatively high, with 54% of pairs producing chicks, compared to 35% for Lapwing and 23% for Curlew.     

鸟类群落研究进展

鸟类群落的研究一直是鸟类生态学研究的重要领域,研究内容主要集中在以下几个方面:群落的组成结构、集团结构、生态位与种间关系、动态与演替、群落与栖息地关系以及城市化对鸟类群落影响。群落的组成与结构、动态与演替以及群落与栖息地关系的研究是过去20年间的研究热点,但有关城市化对鸟类群落影响的研究还比较少。本文在总结国内外鸟类群落生态学研究的基础上,重点讨论了目前的研究热点和今后的发展趋势,以期促进我国鸟类群落生态学的研究。     

Reconstituted basement-membrane matrix modulates fibroblast activities in vitro

Cellular growth and collagen biosynthesis were compared in dermal calf fibroblasts cultured on plastic or on a reconstituted basement membrane gel, termed matrigel. This matrix, extracted from Engelbreth-Holm-Swarm tumors, consists mainly of laminin, entactin, type IV collagen, and heparan sulfate proteoglycan. The multiplication rate of fibroblasts grown on matrigel was stimulated compared to that of monolayered cells cultured on plastic, and these cells formed multilayers after 4 days. Protein and collagen biosynthesis was reduced in fibroblasts cultured on matrigel. A higher proportion of the newly synthesized collagen (40%) was incorporated to the extracellular matrix in cultures grown on matrigel than in those grown on plastic (14%). Type III collagen was the preferential collagen type deposited on matrigel, and the ratio of type III:type I collagens secreted in the medium was also slightly higher in cultures grown on matrigel. Partially processed collagen was more abundant in fibroblasts grown on matrigel than in cells cultured on plastic. Finally, cells grown on matrigel exhibited a higher catabolic activity than cells grown on plastic. In this experimental model, the reconstituted basement-membrane matrix seems to influence the activities of fibroblasts significantly.     

Two new species of Leporinus (Characiformes: Anostomidae) from the Brazilian Amazon,and redescription of Leporinus striatus Kner 1858

Two new species of Leporinus are described from tributaries of the Rio Amazonas in Brazil. One species is known from the Jari and Tapajós River basins, and is identified on the basis of a gas bladder reduced in size, a dark midlateral stripe on the body, dark transverse bars on the dorsum, a subinferior mouth, three teeth on the premaxilla, four teeth on the dentary and 16 scale rows around the caudal peduncle. The second new species is known from the Tocantins, Xingu and Tapajós River basins, and is identified on the basis of three dark longitudinal stripes on the body, a subinferior mouth, three teeth on the premaxilla, four teeth on the dentary and 12 scale rows around the caudal peduncle. In addition, Leporinus striatus is redescribed based on type and additional specimens from the Río de La Plata, Amazonas, Orinoco, Atrato, Magdalena and Sinu River basins. Leporinus striatus is identified on the basis of four dark longitudinal stripes on the body, a subterminal mouth, three teeth on the premaxilla, four teeth on the dentary and 16 scale rows around the caudal peduncle.     

The antitumor action of seminal ribonuclease tested with the plasmacytoma spleen colonization assay

The antitumor action of bovine seminal ribonuclease was evaluated with a quantitative assay based on the production of tumor foci in the spleens of mice injected with plasmacytoma cells. The antitumor action depended on the integrity of the catalytic site, and on the dimeric structure of the enzyme. A working hypothesis is proposed, based on these results, and on previous results obtained studying the antitumor action of seminal RNAase in vitro on cell cultures. According to this hypothesis, the antitumor action is based on the ability of seminal RNAase to interact at specific receptor sites on the tumor cell membrane, as well as on its RNA degrading ability.     

Nymph and adult biology of Dichelops melacanthus (Dallas) (Heteroptera: Pentatomidae) feeding on cultivated and non-cultivated host plants

The biology of the pentatomid Dichelops melacanthus (Dallas) feeding on cultivated and non-cultivated plants was studied in the laboratory. Nymph mortality varied from approximately 60 on corn (seed mature) to 77% on wheat (ear immature); no nymphs survived on seedlings of corn or wheat. Nymph developmental time on soybean, corn or wheat (seed, pod or ear) varied from 25.5 to 32.8 days. Body weight at adult emergence was similar and greater on most foods than on wheat ear. Nymphs fed preferentially on soybean (pod immature). On non-cultivated hosts, nymphs showed high mortality (73%) on crotalaria (pod immature); on tropical spiderwort (stem) all nymphs died. Nymphs took longer time to develop on crotalaria and/or on spiderwort than on soybean. Body weight at adult emergence did not differ on crotalaria or soybean. Survivorship decreased with time on most foods, with approximately 50% of adults alive at day 30. On corn and wheat seedlings approximately 80% of adults were dead on day 20. Adult longevity ranged 31-43 days, except on corn and wheat seedlings < 15 days. Females % ovipositing peaked ( approximately 76%) on soybean (pod or seed immature), and was minimum ( approximately 9%) on wheat ear (immature); no females reproduced on seedlings of corn or wheat. Preoviposition period was shorter ( approximately 12 days) on soybean (immature pod or seed) and longer ( approximately 37 days) on wheat (ear immature). Fecundity was similar and higher on all foods than on wheat (ear immature). Body weigh gain occurred on all foods, but on corn and wheat seedlings. Adults fed preferably on soybean (pod immature and seed mature); wheat (seedling) was the least preferred food.     

异迟眼蕈蚊在不同植物上的生长发育及种群参数

【目的】异迟眼蕈蚊Bradysia difformis Frey的幼虫取食为害作物的地下部分,影响作物的品质,为了明确韭菜、蚕豆、生菜、白菜和甘蓝5种植物对异迟眼蕈蚊生长发育以及繁殖的影响。【方法】本试验采用室内人工饲养测定的方法,研究了5种不同植物对异迟眼蕈蚊生长发育,繁殖力和存活率的影响,并统计了其对异迟眼蕈蚊种群参数的影响。【结果】结果表明:卵到蛹的发育历期依次为甘蓝、白菜、韭菜、生菜、蚕豆;5种植物对雌雄虫寿命影响不显著,对雌虫产卵量以及蛹重均有影响,其中在韭菜上的产卵量最大,甘蓝最少,在韭菜上蛹最重,生菜上蛹最轻;异迟眼蕈蚊的存活率随着生长发育降低,总体在韭菜上的存活率高于其他寄主植物,在生菜上的存活率均最低。统计分析不同植物对异迟眼蕈蚊种群参数的影响,净增殖率和内禀增长率在韭菜上最大而在甘蓝上最小;平均世代周期在蚕豆上最短,甘蓝上最长;种群加倍时间在韭菜上最短,而在甘蓝上最长。【结论】由此可知,异迟眼蕈蚊均可以在韭菜,蚕豆,生菜,白菜和甘蓝上完成生长发育及繁殖,其对5种植物的适应性依次为:韭菜、蚕豆、白菜、甘蓝和生菜。     

Potential adaptation of a Q biotype whitefly population from poinsettia to field crops

Abstract The invasive Q biotype whitefly was first detected in the US on poinsettia in 2004 and is still not a pest outside of greenhouse environments in the US. To assess the potential for the establishment of the Q biotype on field crops, population cage experiments were conducted to compare the performance of a poinsettia‐derived Q population named P’06 on poinsettia and six field crops (alfalfa, tomato, melon, cotton, cowpea and cabbage). P’06 adults reared on poinsettia as nymphs laid eggs on all six field crops. Significantly more eggs were laid on alfalfa, tomato, melon and cotton than on cabbage, cowpea and poinsettia. These eggs hatched and the nymphs developed to adults on the six field crops. Relative to poinsettia, whitefly survival was similar on cowpea, alfalfa, tomato and cabbage, but significantly higher on cotton and melon. Moreover, P’06 had significantly shorter development times from egg to adult on cotton, melon, cowpea, tomato and alfalfa than they did on poinsettia. However, the F1 adults raised on the six field crops had significantly shorter lifespans and laid 11‐ to 18‐fold fewer eggs than did the F1 adults raised on poinsettia. Taken together, while P’06 may have some potential to establish on field crops, the shorter lifespans and extremely low fecundities of the F1 adults raised on the six field crops suggests that P’06 is incapable of rapidly adapting to them. Poor adaptation to field crops may explain, at least partially, why the Q biotype has not established in the US field system.     

Host-plant species modifies the diet of an omnivore feeding on three trophic levels

The diet choice of omnivores feeding on two adjacent trophic levels (either plants and herbivores or herbivores and predators) has been studied extensively. However, omnivores usually feed on more than two trophic levels, and this diet choice and its consequences for population dynamics have hardly been studied. We report how host-plant quality affects the diet choice of western flower thrips feeding on three trophic levels: plants (cucumber or sweet pepper), eggs of spider mites and eggs of a predatory mite that attacks spider mites. Spider mites feed on the same host plants as thrips and produce a web that hampers predator mobility. To assess the indirect effects of spider mites on predation by thrips, the thrips were offered spider-mite eggs and predatory-mite eggs on cucumber or sweet pepper leaf discs that were either clean, damaged by spider mites but without spider-mite web, or damaged and webbed. We show that, overall, thrips consumed more eggs on sweet pepper, a plant of low quality, than on cucumber, a high quality host plant. On damaged and webbed leaf discs (mimicking the natural situation), thrips killed more predator eggs than spider-mite eggs on sweet pepper, but they killed equal numbers of eggs of each species on cucumber. This is because web hampered predation on spider-mite eggs by thrips on sweet pepper, but not on cucumber, whereas it did not affect predation on predatory-mite eggs. We used the data obtained to parameterize a model of the local dynamics of this system. The model predicts that total predation by the omnivore has little effects on population dynamics, whereas differential attack of predator eggs and spider-mite eggs by the omnivore has large effects on the dynamics of both mite species on the two host plants.     

Patterns of species diversity on an oceanic versus a continental island mountain: a hypothesis on species diversification1

Abstract. The vegetation on a wet mountain slope on Haleakala (an oceanic island) is compared with that on Kinabalu (a continental island) to examine relationships between regional floristic richness and α- and β-diversities. The two mountains are similar in their constant tropical climate, generic and family-level floristic elements and geological age of the summit regions, but different in regional floristic richness (rich on Kinabalu vs. poor on Haleakala). α-diversity of canopy and subcanopy tree species was much higher on Kinabalu than in comparable zones on Haleakala. Average turnover rate of species (as logarithmic community similarity) on the slope was one order of magnitude greater on Kinabalu than on Haleakala (0.127 vs. 0.017 per 100 m alt.). While there were genera with wide altitudinal ranges on both mountains, a large proportion of the genera was differentiated into parapatric altitudinal congeners on Kinabalu. By contrast, most genera are altitudinally monotypic on Haleakala. The number of sympatric congeners per genus, and the frequency of multi-specific genera per plot were high on lower slopes but decreased with increasing altitude on Kinabalu, whereas the values were low across all altitudes on Haleakala. These patterns suggest that sympatric and parapatric species radiation was less on Haleakala than on Kinabalu. This may be related to Haleakala's initially poor and disharmonic flora.     

Habitat associations of California ground squirrels and Botta's pocket gophers on levees in California

Vegetation management on levees, especially removal of trees and shrubs, might affect burrowing mammals that are considered threats to levee integrity. We evaluated habitat associations of California ground squirrels (Otospermophilus beecheyi) and Botta's pocket gophers (Thomomys bottae) on levees in the Sacramento Valley to assess the effects of levee vegetation management on these species. Using burrows as an indirect measure of mammal presence, we found that the presence of trees had a negative effect on the occurrence and abundance of ground squirrels on levees, and on the location of their burrowing activities on the levee slope, potentially because visual occlusion caused by tall woody vegetation impedes detection of predators. Similarly, trees had a negative effect on the abundance of pocket gophers on levees and on the location of their burrowing activities on the levee slope, probably because of the effect of tree cover on food availability. The conversion of woodland habitats to grasslands on levees most likely will result in increased occurrence, abundance, or both of ground squirrels and pocket gophers, and thereby increase the potential threat that their burrowing activities pose to levee integrity. © 2012 The Wildlife Society.     

Effect of host-plant manipulation by a gall-inducing insect on abundance of herbivores on chestnut trees

We investigated plant-mediated effects of the stem gall wasp, Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae), on other herbivores on the chestnut tree Castanea crenata. In the early season, leaves emerged earlier and in greater numbers on galled shoots than on ungalled shoots. On galled shoots the leaf to shoot biomass ratio was lower and the leaves were physically different. In May and June the concentration of nitrogen in leaves was higher on galled shoots than on ungalled shoots. In July, the water content of leaves was lower on galled shoots. In May and June, the number of aphids, Myzocallis kuricola Matsumura (Homoptera: Aphidoidea), on leaves was higher on galled shoots than on ungalled shoots, but the opposite was true at the end of July. Laboratory experiments showed that aphid fecundity and body weight decrease were higher in May and June when aphids fed on leaves on a galled shoot than when they fed on those on ungalled shoots. In contrast, aphid performance in July was greater on ungalled leaves than on galled leaves. Our findings suggest that gall initiation in a chestnut tree affected aphid performance by affecting host plant quality.     

浙江海岛鸟兽地理生态学的初步研究

对浙江洞头岛及舟山五岛的鸟兽调查表明,海岛动物的种数较相邻大陆为低,但种群密度却高于大陆,岛上的某些种类出现生态位扩展的现象。舟山五岛兽类的种数和岛屿面积呈正相关,其关系式为S=2.12A~(0.29),但种群密度随着岛屿面积的增大而下降。文中据此提出了保护和发展岛屿动物资源的某些措施。     

Biology and life table studies of the oriental tobacco budworm,Helicoverpa assulta (Lepidoptera: Noctuidae), influenced by different larval diets

Development, survivorship, pupal weight, oviposition, and life table parameters of the oriental tobacco budworm, Helicoverpa assulta Guenée, were evaluated in the laboratory on an artificial diet, pepper (Capsicum frutescens L.), and tobacco (Nicotiana tobacum L.). We found that the average developmental time of immature stages was longest on tobacco (36.2 d), intermediate on pepper (34.4 d), and shortest on artificial diet (33.5 d). Immature survival from egg to pupa varied from 31% on tobacco, 43% on pepper, and 74% on artificial diet. Pupal weight ranged from 197.4 mg/pupa on tobacco, 233.1 mg/pupa on pepper and 253.4 mg/pupa on artificial diet. The average numbers of eggs laid by adults reared as larvae on the artificial diet, pepper, or tobacco were 614, 421 and 334 eggs/female, respectively. Numbers of remaining eggs in ovaries of the adult females reared as larvae on the artificial diet, pepper, or tobacco were 16, 26, and 42 eggs/female, respectively. The longevity of adult females developed from larvae reared on the three diets was not significantly different, whereas the longevity of male adults from the larvae reared on artificial diet was longer (16.8 d) than that for males reared on tobacco (13.8 d) and pepper (13.3 d). The intrinsic, finite, gross, and net rates of increase were highest for females reared as larvae on artificial diet, lowest for females emerging from larvae reared on tobacco, and intermediate for females emerging from larvae reared on pepper. Generation times and doubling time of H. assulta were shortest for larvae fed artificial diet, intermediate from larvae reared on pepper, and longest from larvae reared on tobacco. We concluded that the artificial diet was the most suitable larval diet of H. assulta followed by pepper, and tobacco.     

Rhodnius prolixus vitellogenesis: Dependence upon the blood source

The haemolymph-protein patterns of a Rhodnius prolixus colony, fed on human blood and of a colony fed on sheep blood, were compared by SDS-PAGE analysis. The vitellogenin-polypeptide patterns of larvae and females of both colonies were similar. However, males of the human-blood-fed colony had levels of circulating vitellogenin comparable to those of females, while males of the colony fed on sheep blood had only residual vitellogenin levels. The effect of human blood on different instars of a colony fed on sheep blood was examined. Autogeny occurs in the colony fed on human blood but not on that fed on sheep blood. When 5th-instar females from the sheep-blood-fed colony were fed on human blood, the phenomenon of autogeny was not displayed after moulting to the adult stage. Fourth-instar larvae from the colony fed on sheep blood however showed autogeny in the adult stage when fed on human blood from the 4th-instar on or in this stage only. Furthermore, first-cycle oviposition and the percentage moulting to the adult stage were increased in these groups.     

中国海南省锈菌资料补编

补充记载了采自海南省的9种锈菌。甲竹Bambusa remotiflora上的华南柄锈菌Puccinia austrosinensis、大花紫薇Lagerstroemia speciosa上的紫薇夏孢锈菌Uredo lagerstroemiae和海南薯Ipomoea sumatrana上的宽乳单胞锈菌Uromyces latimammatus为新种;厚叶素馨Jasminum pentaneurum上的巴氏查科锈菌Chaconia butleri、猪肚木Canthium horridum上的鱼骨木驼孢锈菌Hemileia canthii、牛筋果Harrisonia perforata上的牛筋果无眠多胞锈菌Kuehneola harrisoniae、毛咀签Gouania javanica上的椴叶咀签柄锈菌Puccinia gouaniae-tiliaefoliae、蒲桃Syzygium jambos上的番石榴柄锈菌Puccinia psidii以及象草Pennisetum purpureum上的钝叶草柄锈菌Puccinia stenotaphri为中国新记录。引证的标本均保存于中国科学院菌物标本馆（HMAS）。