强力霉素对胃癌细胞SGC-7901基质金属蛋白酶-2和基质金属蛋白酶组织抑制因子-2表达的影响

背景：强力霉素对结肠癌细胞的分化和抑制作用已有报道，但其对胃癌细胞的作用尚未见报道。目的：观察强力霉素对人胃癌细胞SGC-7901的生长抑制作用及其对基质金属蛋白酶（MMP）-2和基质金属蛋白酶组织抑制因子（TIMP）-2表达的影响，探索胃癌治疗的新方法。方法：采用不同浓度的强力霉素作用于胃癌细胞SGC-7901，以噻唑蓝（MIT）法测定其细胞毒作用；逆转录聚合酶链反应（RT—PCR）法半定量测定MMP．2和TIMP-2mRNA的表达；免疫组化法观察MMP-2蛋白的表达。结果：强力霉素可抑制胃癌细胞SGC-7901的生长，具有浓度和时间依赖性（P〈0．01）。强力霉素可下调MMP-2mRNA和MMP-2蛋白的表达，上调TIMP-2mRNA的表达，具有浓度依赖性（P〈0．05）。结论：强力霉素能抑制胃癌细胞SGC-7901的生长，其作用机制可能与下调MMP-2表达、上调TIMP-2表达有关。     

胃癌组织中MMP-9和TIMP-1的表达及其临床意义

背景：有关胃癌组织中基质金属蛋白酶（MMPs）和金属蛋白酶组织抑制剂（TIMPs）表达的研究不多且结果不一。目的：探讨胃癌组织中MMP-9和TIMP-1的表达及其临床意义。方法：收集临床病理资料完整的98例胃癌患者。应用免疫组化法检测组织中MMP-9和TIMP-1表达．并分析其与临床病理特征的关系以及各参数对胃癌患者预后的影响。结果：胃癌组织中MMP-9高表达与浸润深度、淋巴结转移和TNM分期显著相关（P〈0．01）。单因素分析显示胃癌患者预后与MMP-9高表达（P=0．014）、浸润深度（P〈0．001）、淋巴结转移（P〈0．0001）和TNM分期（P〈0．0001）相关。TIMP-1表达与胃癌患者临床病理特征和预后无关。结论：MMP-9高表达与胃癌的发生、进展有关,可作为胃癌患者预后指标之一。     

基质金属蛋白酶在心房颤动犬心房结构重构中的作用

目的 探讨基质金属蛋白酶-9（MMP-9）和组织型基质金属蛋白酶抑制因子-1（TIMP-1）在快速起搏心房颤动（房颤）动物模型心房结构重构中的作用和Ca^2＋超载对MMP-9激活的影响。方法 14只犬随机分为房颤组（n=8）和对照组（n=6），右心房快速起搏（350～450次／min）8周建立房颤动物模型。取左心房组织，采用半定量逆转录聚合酶链反应（RT-PCR）和免疫组织化学法检测MMP-9和TIMP-1 mRNA和蛋白质表达，采用Masson染色测定心房肌组织胶原含量，采用超声心动图测量左心房内径，同时还测定心房肌组织Ca^2＋浓度。结果 与对照组比较，房颤组心房肌胶原含量、Ca^2＋浓度和左心房内径增加（P〈0．05）；房颤组左心房心肌组织MMP-9 mRNA表达升高45％（P〈0．01），蛋白质水平表达增加19．5％（P〈0．001）；TIMP-1 mRNA表达增加46．67％（P〈0．01），TIMP-1蛋白质水平表达下调8．33％（P〈0．01）；MMP-9 mRNA表达与左心房内径、Ca^2＋浓度和心肌胶原含量正相关（P〈0．05）；TIMP-1 mRNA表达与左心房内径、心肌胶原含量和MMP-9 mRNA表达正相关（P〈0．05）。结论 MMP-9／TIMP-1平衡失调可能是慢性房颤心房肌细胞外基质重构和心房扩大的重要分子机制，细胞内Ca^2＋超载可能是MMPs的重要激活途径。     

MMP-2、TIMP-2在脑膜瘤组织中的表达及临床意义

目的探讨基质金属蛋白酶-2（MMP-2）与基质金属蛋白酶组织抑制因子-2（TIMP-2）在脑膜瘤组织中的表达及意义。方法选择恶性脑膜瘤标本15份（恶性组）,良性脑膜瘤标本20份（良性组）,采用免疫组化sP法检测两组MMP-2和TIMP-2表达情况,采用spe蛐an等级相关分析法检测两指标的相关性。结果恶性组及良性组MMP-2阳性表达率分别为73．3％（11／15）、15％（3／20）,TIMP-2阳性表达率分别为13．3％（2／15）、80％（16／20）,两组比较P均〈0．01。脑膜瘤中MMP-2与TIMP-2表达呈负相关（r=-0．545,P〈0．01）。结论MMP-2可作为判断肿瘤恶变的指标,TIMP-2可作为肿瘤非恶变的指标,两指标检测可用于指导脑膜瘤的治疗及预后评估。     

血管紧张素Ⅱ对大鼠肾小球系膜细胞MMP-2和TIMP-2 mRNA表达的影响

正常大鼠肾小球系膜细胞的实验研究显示，血管紧张素Ⅱ（ATⅡ）对其质金属蛋白酶-2（MMP-2）的mRNA表达起降调节作用，对MMP-2基质金属蛋白酶组织抑制物-2（TIMP-2）的mRNA表达无明显影响。结果是，ATⅡ对MMP-2mRNA／TIMP-2mRNA二者表达的比值，起到降调节作用。     

MMP-2、TIMP-2在食管鳞状细胞癌中的表达及其临床意义

目的探讨基质金属蛋白酶2（MMP-2）、基质金属蛋白酶组织抑制剂2（TIMP-2）与食管鳞状细胞癌（ES-CC）生物学行为之间的关系。方法采用免疫组化法检测MMP-2、TIMP-2在ESCC中的表达。结果有无淋巴结转移、不同浸润深度、不同临床分期的ESCC患者MMP-2的阳性表达率有统计学差异（P均〈0.05）,有无淋巴结转移、不同临床分期患者的TIMP-2阳性表达率也有统计学差异（P均〈0.05）。结论MMP-2、TIMP-2平衡破坏是ESCC发生、侵袭、转移、甚至影响预后的重要因素,二者均可作为判断ESCC恶性生物学行为的标记物。     

灵芝多糖对糖尿病大鼠肾组织MMP-2和TIMP-2表达的影响

目的观察基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶抑制因子-2（TIMP-2）在糖尿病大鼠肾组织中的表达及灵芝多糖干预后的影响。方法腹腔注射链脲佐菌素（STZ）诱导制备糖尿病大鼠模型，分组给予不同剂量灵芝多糖（GLP，100、200、400mg／kg）进行治疗性灌胃。8w后，免疫组化和RT-PCR方法雄测各组大鼠肾皮质MMP-2、TIMP-2的表达。结果糖尿病组大鼠肾小球MMP-2的表达较正常对照组明显减少，TIMP-2明显升高（P〈0．01）；灵芝多糖各组较糖尿病组MMP-2表达升高（P〈0．01），TIMP-2表达减少（P〈0．01）。结论灵芝多糖通过调节MMP-2／TIMP-2的平衡，减少细胞外基质积聚，对糖尿病大鼠肾脏起保护作用。     

MMP-9及TIMP-1在支气管哮喘发病中作用及雷公藤多甙干预研究

目的观察支气管哮喘（简称哮喘）大鼠肺组织中细胞外基质（ECM）代谢相关因子基质金属蛋白酶9（MMP-9）及其抑制剂组织金属蛋白酶抑制剂1（TIMP-1）的表达，研究雷公藤多甙在哮喘肺组织ECM重塑中可能的作用及其机制。方法建立大鼠哮喘模型，采用逆转录-聚合酶链反应技术（RT-PCR）测定肺组织中MMP-9、TIMP-1mRNA的表达。结果哮喘组MMP-9、TIMP-1在肺组织中的蛋白表达明显高于对照组（P〈0．01），应用药物雷公藤多甙干预后，MMP-9、TIMP-1蛋白表达明显低于哮喘组（P〈0．05）。哮喘组MMP-9、TIMP-1在肺组织的mRNA表达也高于对照组（P〈0．01），应用雷公藤多甙药物干预后，MMP-9、TIMP．1在肺组织的mRNA表达明显低于哮喘组（P〈0．01）。哮喘组肺组织中MMP-9／TIMP-1〉1，明显高于对照组（P〈0．05），应用药物雷公藤多甙干预后，MMP-9／TIMP．1〈1，明显低于对照组（P〈0．05）和哮喘组（P〈0．01）。结论雷公藤多甙可能下调MMP-9的表达，调节MMP-9／TIMP-1的平衡，干预细胞外基质重塑。     

ERK通路抑制剂对白蛋白诱导的人近端肾小管上皮细胞细胞外基质合成的影响

目的探讨血清白蛋白在肾小管间质纤维化（TIF）发生、发展中的作用。方法将体外培养的人近端肾小管上皮细胞株HK-2细胞随机分为四组：A组不加刺激物;B组加入5mg／ml人血清白蛋白;C组加入10punol／L的PD98059;D组用10p,mol／LPD98059预处理HK-2细胞45min后再加入5mg／m1人血清白蛋白。RT—PCR法检测各组HK-2细胞在0、12、24、48h基质金属蛋白酶-9（MMP-9）、金属蛋白酶组织抑制剂-1（TIMP-1）和Ⅳ型胶原（col-Ⅳ）mRNA相对表达量。结果B组MMP-9mRNA表达先升高后下降,TIMP-1mRNA、col—ⅣmRNA表达均呈时间依赖性升高,TIMP-1／MMP-9值先下降后升高,D组MMP-9、TIMP-1和col-ⅣmRNA表达均明显低于B组,P均〈0．01;TIMP-1／MMP-9值的失衡得到部分纠正（P〈0．05或〈0．01）。结论人血清白蛋白可能通过ERK通路作用于HK-2细胞,促进ECM合成和抑制ECM降解、诱导ECM积聚而促进TIF发生、发展。     

心房颤动患者血清基质金属蛋白酶-2及其组织抑制因子浓度的变化

目的：研究非瓣膜性心房颤动（NVAf）患者血清基质金属蛋白酶-2（MMP-2）与基质金属蛋白酶组织抑制因子-2（TIMP-2）的水平．探讨NVAf发生的可能机制。方法：采用定量酶联免疫吸附试验（ELISA）测定30例NVAf患者（房颤组）和30例正常人（正常对照组）血清MMP-2、TIMP-2的水平。结果：房颤组血清MMP-2水平（390．29±128．67ng／ml）明显高于正常对照组（291.80±104．15ng／ml）水平（P〈0．01）。房颤组血清TIMP-2水平（62．84±26．77ng／ml）明显低于正常对照组（115．22±45．00ng／ml）,P〈0．01,血清MMP-2水平与左房直径呈正相关（r=0．396,P〈0．01）。多因素非条件Logistic回顾模型中左房直径、MMP-2是NVAf发生的危险因素,TIMP-2是保护免于发生房颤的因素。结论：MMP-2与TIMP-2可能参与房颤的发生发展。     

Matrix metalloproteinase-2 and tissue inhibitor of metallo-proteinase-2 in colorectal carcinoma invasion and metastasis

AIM: To explore the relationship between matrix metallopr- oteinase-2 (MMP-2) and tissue inhibitor of metallopr- oteinase-2 (TIMP-2) in the development of colorectal carcinoma and to provide a valuable marker for clinical diagnosis. METHODS: Twenty-five patients with colorectal carcinoma underwent surgical resection. Samples were taken from tumor sites and normal tissues. MMP-2 activity was determined by gelatin zymography. Western blot and ABC immunohist-ochemical staining were used to detect the expression levels of MMP-2 and TIMP-2 in normal and colorectal carcinoma tissues. Statistical analyses were performed using the Student's t test and one-way ANOVA. P<0.05 was considered statistically .significant. All the statistical analyses were performed using SPSS 10.0 software. RESULTS: MMP-2 activity could be detected in both normal and colorectal carcinoma tissues. MMP-2 activity in colorectal carcinoma tissues was much higher than that in normal tissues (P<0.05, t=3.916,4.227). MMP-2 activity was positively related to the colorectal carcinoma invasion depth, lymph node metastasis and Duke's stage. Western blot and ABC immunohistochemical staining demonstrated that the expression level of MMP-2 in colorectal carcinoma tissues was much higher than that in normal tissues (P<0.05, t = 9.429), but the expression level of TIMP-2 in colorectal carcinoma tissues was much lower than that in normal tissues (P<0.05, t = 7.329). The MMP-2/TIMP-2 ratio of colorectal carcinoma was much higher than that of normal tissues. With the progression of invasion depth, lymph node metastasis and tumor Duke's stage, the activity and expression level of MMP-2 and TIMP-2 gradually increased, but the MMP-2/TIMP-2 ratio gradually decreased. CONCLUSION: The balance between MMP-2 and TIMP-2 plays a crucial role in the process of colorectal carcinoma invasion and metastasis.     

DNA ploidy analysis and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma

AIM: To investigate DNA ploidy and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma and to explore the mechanism of invasion and metastasis of gastric carcinoma. METHODS: Immunohistochemical methods were used to detect the expressions of MMP-9, TIMP-2, and E-cadherin in 156 cases, including 99 cases of gastric carcinoma, 16 cases of adjacent noncancerous mucosa, 16 cases of distant metastases and 25 cases of metastatic lymph node (LN) from gastric carcinoma. Flow cytometry DNA ploidy and S-phase fraction (SPF) analysis were performed on 57 cases, including 47 cases of gastric cancer, 6 cases of adjacent noncancerous mucosa, and 4 cases of distant metastatic cancer. RESULTS: The expression of MMP-9 was significantly correlated with Lauren's classification, Borrmann's classification, LN metastasis, tumor metastasis, and TNM stage, as well as depth of invasion (all P<0.05). The positive rate was lower in noncarcinoma than in carcinoma (31.3% vs66.7%, P<0.01). The expression of TIMP-2 was significantly correlated with Borrmann's classification, LN metastasis, and the depth of invasion (all P<0.05), The expression of E-cadherin was significantly correlated with differentiation, Lauren's classification, Borrmann's classification, and LN metastasis, as well as the depth of invasion (P<0,01 or P<0.05). E-cadherin was less expressed in carcinoma than in noncarcinoma (42.4% vs87.5%, P<0.01). There was a positive correlation between MMP-9 and TIMP-2 and a negative correlation between MMP-9 and E-cadherin, but no correlation between TIMP-2 and E-cadherin. Also there was a positive correlation between DNA aneuploid rate and differentiation and LN metastasis. SPF that was higher than 15% was positively correlated with tumor size, differentiation and LN metastasis. And there was a significant difference between carcinoma and noncarcinoma in DNA aneuploid rate and SPF. CONCLUSION: With tumor progression and development of heterogeneity, the abnormal expressions of MMP-9, TIMP-2, and E-cadherin or DNA aneuploid rate or high SPF gradually increases, suggesting that they play a crucial role in gastric carcinoma progression.     

E-钙粘素、基质金属蛋白酶-2及其抑制剂与大肠癌浸润转移的关系

目的:探讨E-钙粘素(E-Cad)、基质金属蛋白酶-2(MMP-2)及其抑制剂(TIMP-2)表达与大肠癌浸润转移的关系。方法:采用S-P免疫组织化学染色技术,检测30例大肠腺癌,60例大肠癌组织E-Cad、MMP-2和TIMP-2的表达情况。结果:E-Cad的表达率在大肠腺瘤中为87.10%,显著高于大肠癌中的55.10%(P<0.05);其表达与大肠癌的大体类型有关,且随着大肠癌分化程度的降低而减少,与淋巴结转移呈负相关,E-Cad表达率越高患者的预后越好(P均<0.05)。MMP-2的表达率在大肠腺瘤中为26.67%,显著低于大肠癌中的86.67％(P<0.05);其表达与大肠癌的Dukes分期、分化程度、淋巴结转移和生存期均密切相关(P均<0.05)。TIMP-2的表达在大肠腺癌和大肠癌组织中没有显著性差异(P均>0.05),但其表达与大肠癌的Dukes分期、淋巴结转移、远隔脏器转移及生存期均有关(P均<0.05)。结论:E-Cad、MMP-2和TIMP-2的检测可以成为临床判断大肠癌的恶性程度、转移及预后的重要参考指标。     

The expression of matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinases (TIMPs) and angiogenesis in relation to the depth of tumor invasion and lymph node metastasis in submucosally invasive colorectal carcinoma]

BACKGROUND/AIMS: Lymph node (LN) metastasis occurs in approximately 10% of patients with submucosally invasive colorectal carcinoma. This study was performed to determine the role of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs) production and microvessel formation on the LN metastasis in submucosally invasive colorectal carcinoma. METHODS: A total of forty-one subjects with surgically resected submucosally invasive colorectal carcinoma were included in this study. Immunohistochemical staining of MMP-2, MMP-9, TIMP-1, TIMP-2, and urokinase-type plasminogen activator were performed. Angiogenesis was evaluated by counting the number of microvessels in each pathologic specimen as identified by CD34 immunohistochemical staining. RESULTS: The depth of submucosal invasion was not significantly correlated with the expression of MMP-2, MMP-9, TIMP-1, TIMP-2, or urokinase-type plasminogen activator, but the microvessel count was significantly correlated with the absolute depth of invasion (r=0.312, p<0.05). Upregulation of TIMP-2 was positively correlated with adjacent lymphatic invasion (p<0.05) and increased TIMP-2 expression was correlated with LN metastasis in submucosally invasive colorectal carcinoma (p=0.088). CONCLUSIONS: These results suggest that the expression of TIMP-2 and the microvessel count may be useful parameters for considering additional surgery after endoscopic treatment of submucosally invasive colorectal carcinoma.     

Expression of MMP—2,TIMP—2 protein and the ratio of MMP—2／TIMP—2 in gallbladder carcinoma and their significance

AIM: To study the correlation between expression of MMP-2, TIMP-2 protein and the ratio of MMP-2/TIMP-2 and clinicalpathological parameters of patients with gallbladder carcinoma.METHODS: Carcinomas (n=45) and polypoid lesions (n=15) of the gallbladder were studied for the expression of MMP-2 and TIMP-2 protein by immunohistochemical avidin-biotin-complex method and image analysis. Clinicalpathological data of patients with gallbladder carcinoma such as histological type, grade of differentiation, level of infiltration, liver invasion and lymph node involvement, etc, were recorded.RESULTS: There was significant difference between the average level (1.123±0.108 VS 1.030±0.054, P=0.002) of MMP-2, the ratio (1.050±0.013 VS0.937±0.078, P=0.003) of MMP-2/TIMP-2 in gallbladder carcinomas and in polypoid lesions of the gallbladder. Significant difference was found between the expression of MMP-2 in early stage and advanced tumors, but there was no correlation between MMP-2 protein expression and histological type, differentiation degree, infiltration level, lymph node involvement or liver invasion. Although no difference was observed between TIMP-2 expression and histological type or differentiation degree, signific ant difference was found between TIMP-2 expression and different Nevin stage, infiltration level, local lymph node involvement or liver invasion (1.168±0.067 VS1.048±0.075, 1.170±0.062 vs 1.039±0.06g, 1.039±0.076 VS1.147±0.083, 1.048±0.074 vs 1.103±0.095, P＜0.05). MMP-2/TIMP-2 ratio did not correlate with histological type, grade of differentiation and liver invasion, but significant differences were found between MMP-2/TIMP-2 ratio and different Nevin stage, infiltration level and lymph node involvement in patients with carcinoma of gallbladder.CONCLUSION: TIMP-2 and MMP-2/TIMP-2 ratio could reflect more accurately biological characteristic of gallbladder carcinoma and MMP-2/TIMP-2 ratio might be a new significant marker in early diagnosis, in the judgment of invasion or metastasis and the estimate of prognosis in patients with gallbladder carcinomas.     

兔主动脉球囊损伤后MMP-2和TIMP-2 mRNA的动态变化

目的 :探讨基质金属蛋白酶 2 (MMP 2 )、基质金属蛋白酶组织抑制因子 2 (TIMP 2 )核糖核酸 (mR NA)在主动脉球囊损伤后的改变及可能作用。方法 :在兔腹主动脉球囊损伤的模型上 ,应用RT PCR的方法 ,观察MMP 2、TIMP 2mRNA在球囊损伤后不同时间的表达情况。结果 :MMP 2mRNA在球囊损伤后第 1天表达开始升高 ,第 7天达高峰。TIMP 2mRNA球囊损伤后第 1天表达开始逐渐升高 ,第 2 8天达高峰。结论 :MMP 2mRNA在球囊损伤后表达第 7天达高峰 ,在再狭窄早期平滑肌细胞的迁移与增殖中起重要作用 ;TIMP 2mRNA球囊损伤后表达第 2 8天达高峰 ,可能与再狭窄中、后期新生内膜形成及血管重塑有关。     

Imbalance between expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in invasiveness and metastasis of human gastric carcinoma

AIM: The expressive balance between matrix metalloproteinase-9 (MMP-9) and its tissue inhibitor of metalloproteinase-1 (TIMP-1) plays a critical role in maintaining the degradation and synthesis of extracellular matrix. Loss of such balance is associated with invasion and metastasis of tumors. This study aimed to determine the expression of MMP-9 and TIMP-1 in gastric carcinoma, and the association of the expressive imbalance between MMP9 and TIMP-1 with the invasion and metastasis and prognosis of gastric carcinoma.METHODS: We used immunohistochemistry to determine the expressions of MMP-9, TTMP-1 and proliferating cell nuclear antigen Ki-67 in the gastric specimens taken from 256 patients with primary gastric carcinoma. The patients were followed-up for up to 96 months.RESULTS: No association between the expression of MMP9 and TIMP-1 and patients' sex and age, tumor size and location of gastric carcinoma was observed. The incidence of the positive expression of MMP-9 in cases with tumors invasion to muscularis propria and visceral peritoneum (70.13% and 69.09%, respectively) was significantly higher than that in cases with tumor invasion only to lamina propria or submucosa (42.50 %, P=0.0162). The positive correlation between MMP-9 expression and the depth of tumor invasion was observed (Pearson correlation coefficient=0.2129,P=0.016). Along with the increase of the metastatic station of lymph nodes, the incidence of the MMP-9 expression was increased by degrees; a positive correlation between them was observed (Pearson correlation coefficient=0.2910,P=0.0001). There was also a significant correlation between MMP-9 expression and the TNM stage in gastric carcinoma (Pearson correlation coefficient=0.3027, P＜0.0001). The incidence of MMP-9 expression in stage Ⅱ and Ⅲ/Ⅳ (75.00%and 76.15%, respectively) was significantly higher than those in stage Ⅰ (46.15 %, P＜0.0001). A negative correlation between TIMP-1 immunoreactivity and the depth of invasion,status of lymph node metastasis and TNM stage was observed (Pearson correlation coefficient =-0.1688, -0.3556and -0.3004, P=0.023, ＜0.0001 and ＜0.0001, respectively).Four types of co-expression of MMP-9 and TIMP-1 were observed; i.e. MMP-9 positive but T IMP-1 negative (n=115),both positive (n=52), both negative (n=62) and MMP-9negative but TIMP-1 positive (n=27). The frequency of serosal invasiveness was significant higher in patients with MMP-9 but without TIMP-1 expression than those with other types of the co-expression (P=0.0303). The incidence of lymph node metastasis was highest in patients with MMP-9but without TIMP-1 expression, and lowest in those with TIMP-1 but without MMP-9 expression (P＜0.0001). The survival rate in patients with MMP-9 but without TIMP-1expression was lower than that in those with TIMP-1 but without MMP-9 expression (P=0.0014).CONCLUSION: Our results in gastric carcinoma demonstrated a significant positive association of MMP-9 over-expression with proliferation of tumor cells, the depth of invasiveness,lymph node metastasis and TNM stage, suggesting MMP-9can serve as a molecular marker of tumor invasion and metastasis. We also demonstrate a significant negative relationship of TIMP-1 expression with the depth of invasiveness and lymph node metastasis, which provide a new idea in the tumor biological and genetic treatment.The interaction between MMP-9 and TIMP-1 in the processes of tumor invasion and metastasis is that MMP-9 mainly promotes tumor invasion and metastasis and TIMP-1 inhibits functions of MMP-9. The imbalance between MMP-9 and TIMP-1 expression may suggest the occurrence of tumor invasion and metastasis, predict poor prognosis. For patients with imbalanced MMP-9 and TIMP-1 expression, the optimal treatment scheme needs to be selected.     

血管生成素-2与基质金属蛋白酶-7在大肠癌中的表达及意义

目的:研究血管生成素-2(Ang-2)及基质金属蛋白酶-7(MMP-7)蛋白在人大肠癌组织中的表达及其与大肠癌临床病理特征的关系．方法:应用免疫组化法检测40例大肠癌及其癌旁正常组织中Ang-2及MMP-7蛋白表达水平．结果:大肠癌组织中Ang-2蛋白表达阳性率为77．5％(31/40),明显高于癌旁正常组织 40％(16/40)(P=0．001);Ang-2表达水平与患者性别、年龄及癌组织的部位、大小、分化程度、淋巴结转移无关(P>0．05);与浸润深度, 远处转移及Dukes’分期相关(分别为P=0．007, P=0．023,P=0．008);大肠癌组织RMMP-7蛋白表达阳性率为85％(34/40),明显高于癌旁正常组织35％(14/40)(P=0．000)．MMP-7蛋白表达阳性率与Ang-2的表达相关(P=0．016)．结论:Ang-2蛋白可能通过促进MMP-7的表达从而促进了大肠癌的生长及转移．     

Expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in ulcerative colitis

INTRODUCTION Ulcerative colitis (UC) is a chronic, non-specific inflammatory disease of the colonic mucosa with unknown etiology and pathogenesis. Pathologically, it is characterized by ulceration in the mucosal and submucosal areas, and degradation of ex…