非小细胞肺癌P53、VEGF表达及其与肿瘤血管形成的关系

目的 :探讨P5 3和血管内皮生长因子 (VEGF)在非小细胞肺癌 (NSCLC)中的表达及其与肿瘤血管形成的关系。方法 :用免疫组织化学方法 (S -P法 ) ,检测 76例非小细胞肺癌患者手术切除标本P5 3、VEGF蛋白表达 ,并用Ⅷ因子抗体标记瘤组织中血管内皮细胞 ,计数MVD。结果 :非小细胞肺癌区P5 3、VEGF的阳性表达率及MVD值分别为 :5 5 .2 6 % ,36 .84 % ,32 .6 1± 8.14。非小细胞肺癌组织P5 3和VEGF的阳性表达率与淋巴结转移及临床分期密切相关。P5 3(+)或VEGF(+)组MVD (37.5 6± 6 .2 8,37.37± 7.98)均显著高于P5 3(- )或VEGF(- )组 (2 7.0 0± 5 .13,2 9.5 3± 5 .83;P <0 .0 1) ;VEGF和P5 3均为阳性时 ,MVD值最大 (4 3.5 3± 5 .0 1,P <0 .0 1)。MVD与P5 3蛋白及VEGF阳性表达、VEGF阳性表达与P5 3蛋白阳性表达均呈正相关。结论 :在非小细胞肺癌血管生成过程中 ,P5 3基因、VEGF在调控肿瘤血管形成方面起重要作用。     

大肠癌血管内皮生长因子的表达与微血管密度的研究

目的:探讨大肠癌血管内皮生长因子(VEGF)的表达,微血管生成及其与临床病理参数的关系。方法:采用组织芯片及S-P免疫组化法应用VEGF多克隆抗体、CD34多克隆抗体,检测30例大肠腺癌及10例正常大肠组织中VEGF的表达,计数微血管密度(MVD)。结果:大肠癌中VEGF表达阳性率明显高于正常的大肠黏膜,分别为90%和20%,P<0.05。大肠癌中的MVD明显高于正常大肠黏膜,分别为:59.8±8.7和18.2±1.7,P<0.05。VEGF阳性者MVD显著高于VEGF阴性者,VEGF表达率和MVD与大肠癌Dukes分期、浸润深度密切相关,P<0.05。结论:VEGF表达与大肠癌血管生成有关,大肠癌的发生发展依赖血管生成。MVD可作为大肠癌的预后因素。     

非小细胞肺癌组织中环氧化酶-2、血管内皮生长因子及微血管密度检测

目的:检测非小细胞肺癌组织(NSCLC)中环氧化酶-2(COX-2)、血管内皮生长因子(VEGF)及微血管密度(MVD)的表达情况.方法:应用免疫组织化学技术检测80例NSCLC和20例肺良性病变组织中COX-2、VEGF及MVD的表达情况.结果:NSCLC组织中COX-2、VEGF的阳性表达率分别为56.25%、63.75%,MVD值为42.67±10.58,均高于肺良性病变组织的5.00%、10.00%、12.34±7.46,差异有统计学意义(P均<0.05).NSCLC组织中COX-2、VEGF表达具有相关性,与组织分型、分化程度、淋巴结转移有关(P均<0.05).NSCLC组织中MVD随COX-2、VEGF表达增强而增高(P均<0.05).结论:COX-2、VEGF 2者均参与非小细胞肺癌的发生发展,协同促进肺癌的肿瘤血管生成,增加微血管密度.从而促进肺癌的浸润转移.     

肺癌组织中血管内皮生长因子的表达

目的:观察肺癌组织中血管内皮生长因子(VEGF)的表达。方法:采用免疫组化方法(LSAB法)检测114例肺癌组织(腺癌50例,鳞癌42例,腺鳞癌22例;低分化癌41例,中分化癌48例,高分化癌25例;PTNM分期:Ⅰ+Ⅱ期56例,Ⅲ+Ⅳ例期58例;有淋巴结转移66例,无淋巴结转移48例)、相应的癌旁组织及30例肺良性病变组织中的VEGF的表达水平。结果:①肺癌组织中VEGF阳性细胞率(78.70±17.11)%高于肺癌旁组织(46.76±21.97)%和肺良性病变组织(17.70±15.53)%(P均<0.01),肺癌旁组织高于肺良性病变组织(P<0.05);②VEGF表达水平与肺癌肿块大小、淋巴结转移、PTNM分期、细胞分化程度均有关(P<0.05)。结论:VEGF参与了肺癌的发生、发展、转移,可作为评估肺癌生物学行为的一项指标。     

基质金属蛋白酶-2、血管内皮生长因子与卵巢上皮性肿瘤血管生成的关系

目的:探讨基质金属蛋白酶2(MMP2)、血管内皮生长因子(VEGF)的表达与卵巢上皮性肿瘤血管生成的关系及其临床意义。方法:应用免疫组化SABC法检测20例卵巢良性上皮性肿瘤组织、20例卵巢交界性上皮性肿瘤组织、50例卵巢上皮性癌组织中MMP2、VEGF的表达及微血管密度(MVD)。结果:MMP2表达与卵巢癌的临床分期有关(P<0.05);MMP2表达阳性组的MVD值明显高于阴性组的MVD值(P<0.05),卵巢癌组织中MMP2与VEGF的表达呈显著性正相关(rs=0.600,P<0.05)。结论:MMP2与卵巢肿瘤的血管生成有关,检测MMP2的表达可作为反映卵巢癌侵袭和转移潜能的生物学指标。     

血管内皮生长因子和微血管密度在结直肠癌中的意义

目的　探讨血管内皮生长因子 (VEGF)和微血管密度 (MVD)在结直肠癌中的表达以及它们之间的关系。方法　应用免疫组织化学S P法检测 87例结直肠癌组织中血管内皮生长因子的表达以及微血管密度。结果　 87例结直肠癌组织中VEGF的表达阳性率为 6 6 .7% ,MVD为 32 .80± 12 .11。VEGF和MVD在侵及浆膜层的结直肠癌中明显高于侵及肌层者 ,在淋巴结转移阳性组高于淋巴结转移阴性组 ,在DukesC、D期明显高于A、B期 ,差异均有显著性 (P <0 .0 5 ) ,且随着肿瘤分化程度的降低而增高 (P <0 .0 5 ) ;VEGF阳性组中的MVD值显著高于VEGF阴性组 (P <0 .0 1)。结论　VEGF与结直肠癌血管生成密切相关 ,VEGF的表达和MVD可作为判断结直肠癌恶性程度的生物学指标     

血管内皮生长因子及基质金属蛋白酶在非小细胞肺癌中的表达及与微血管密度的相关性研究

目的:研究血管内皮生长因子(VEGF)、基质金属蛋白酶(MMP-2)和微血管密度(MVD)与非小细胞肺癌(NSCLC)的相关性。方法:应用免疫组化法检测VEGF、MMP-2在非小细胞肺癌组织及正常肺组织中的表达水平并计数微血管密度。结果:NSCLC组织中VEGF及MMP-2阳性表达率和MVD值显著高于正常肺组织(P<0.05),VEGF、MMP-2的阳性表达率和MVD值与非小细胞肺癌临床分期和淋巴结转移具有相关性,与肿瘤病理类型无关。结论:非小细胞肺癌组织中VEGF、MMP-2及MVD明显升高,与临床分期及有无淋巴结转移有关,对判断预后有重要意义。     

非小细胞肺癌中血管内皮生长因子表达及与预后的关系

目的　探讨非小细胞肺癌 (NSCLC)中血管内皮生长因子 (VEGF)的表达和微血管密度 (MVD)的关系及与预后的相关性。方法　采用免疫组化方法 ,检测 6 0例NSCLC中VEGF的表达及MVD ,并以Log rank曲线检测其生存率。结果　 6 0例NSCLC中 ,VEGF表达的阳性率为 78.33% ,明显高于正常的肺组织 (4 0 % )。VEGF的表达与性别、年龄、肿物大小、病理类型等无关 (P >0 .0 5 ) ,而与MVD、淋巴结转移有关 (P <0 .0 5 )。在NSCLC中 ,VEGF阳性组的MVD〔(4 9.77± 11.9)条〕明显高于阴性组〔(31.6 7± 8.88)条〕。有淋巴结转移组的VEGF阳性率 (90 % )明显高于无淋巴结转移组 (6 6 .6 7% )。经生存曲线Log rank检测分析表明 ,VEGF表达与NSCLC的预后呈负相关。结论　VEGF的表达对肿瘤血管生成、转移起重要作用 ,与NSCLC预后密切相关 ,可以作为估计生存率的参考指标。     

大肠癌组织血管生成与患者外周血CK20mRNA表达的临床研究

目的观察大肠癌组织中微血管密度、血管内皮细胞生长因子(VEGF)表达与大肠癌患者外围血癌细胞微转移的关系.方法采用S-P免疫组织化学法检测了58例大肠癌组织中的微血管密度(MVD)和VEGF的表达,用巢式RT-PCR法检测了大肠癌患者外周血中癌细胞微转移情况并分析其与病理指标的关系.结果 58例大肠癌组织中35例高中分化MVD计数为30.2±12.7,低分化大肠癌23例MVD计数为86.6±19.1;26例大肠癌组织中VEGF呈阳性染色(44.8%).MVD计数及VEGF阳性表达与大肠癌分化程度、Dukes分期、有无区域淋巴结转移及远处转移密切相关(P<0.05或P<0.01).58例大肠癌患者外周血中32例CK20mRNA阳性表达(32/58),术后48 h有35例阳性表达(35/58),其中根治性切除组有21例阳性表达(21/44),非根治性手术组12例阳性表达(12/14),21例根治性切除患者术后7～14 d有11例转为阴性,12例非根治性切除患者术后7～14 d有11例阳性表达.CK20mRNA阳性表达与大肠癌病理分化程度无关,但与Dukes分期、区域淋巴结转移及远处转移情况相关(P<0.01).CK20mRNA阳性表达患者大肠癌组织中MVD计数(88.5±15.1)与VEGF阳性表达率(65.6%)明显高于CK20mRNA阴性表达患者的MVD计数(31.0±12.9)和VEGF阳性表达率(19.2%)(分别P<0.01,P<0.001).结论大肠癌组织中MVD和VEGF阳性表达与大肠癌的病理分化程度、Dukes分期、淋巴结及远处转移情况相关.大肠癌组织血管生成与周围静脉血中癌细胞微转移情况密切相关.CK20mRNA的检测对大肠癌预后判断、疗效评估及指导综合治疗有重要意义.     

KiSS-1和VEGF在涎腺黏液表皮样癌中的表达及其对血管生成的影响

目的 研究肿瘤转移抑制基因KiSS-1和血管内皮生长因子(VEGF)在涎腺黏液表皮样癌(MEC)组织中的表达情况及其对血管生成的影响.方法 选择河北北方学院附属第一医院口腔科2007年6月至2016年6月收治的68例MEC患者为研究对象.应用免疫组织化学SP法检测68例MEC组织、20例正常涎腺组织中KiSS-1和VEGF的表达,统计学比较其在不同临床病理特征的MEC组织中表达阳性率的差异.光镜下计数利用CD105标记的微血管密度(MVD),统计学比较不同临床病理特征的MEC组织MVD值的差异,并观察KiSS-1和VEGF的表达对MEC组织MVD值的影响.结果 68例MEC组织中KiSS-1的阳性表达率为38.24%,明显低于正常涎腺组织的70.00%,VEGF的阳性表达率为70.59%,明显高于正常涎腺组织的10.00%,差异均有统计学意义(P<0.05).KiSS-1的表达与MEC的淋巴结转移和TNM分期相关(P<0.05),VEGF的表达与MEC的病理分级、淋巴结转移和TNM分期相关(P<0.05);KiSS-1阳性组MVD值为(29.50±9.14),明显低于阴性组的(35.24±10.45),VEGF阳性组MVD值为(35.33±10.20),明显高于阴性组的(27.57±8.45),差异均有显著统计学意义(P<0.01);KiSS-1的表达与VEGF表达呈负相关(rs=-0.488,P=0.000).结论 在MEC组织中KiSS-1表达的下调可能促进VEGF的表达,从而促进了MEC组织血管的生成,促进其侵袭和转移.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.