肥胖SD大鼠肝脏组织中SOCS3 mRNA的表达

[目的]初步探讨肥胖SD大鼠肝脏组织中细胞因子信号传导抑制因子3（suppressor of cytokine signaling 3,SOCS3）mRNA的含量与高瘦素（leptin）血症的关系。[方法]采用高脂饲料喂养以诱导的肥胖（DIO）SD大鼠模型,应用放射免疫分析法检测血清leptin水平,应用RT-PCR半定量法检测肝脏组织中SOCS3 mRNA的含量,并进行相关分析。[结果]DIO组大鼠存在高leptin血症,其肝脏组织中SOCS3 mRNA的含量0.9392±0.0081明显高于正常饮食组大鼠0.3460±0.0743,两组间差异有显著性意义（P〈0.01）,而且肝脏组织中SOCS3 mRNA含量与大鼠的肥胖程度及血清leptin水平呈显著正相关,P〈0.01。[结论]肝脏组织中SOCS3可能是DIO大鼠的leptin抵抗的机制之一。     

电针对食源性肥胖大鼠下丘脑自噬相关蛋白7表达的影响

【目的】探讨电针对食源性肥胖大鼠下丘脑自噬相关蛋白7(Autophagy-related protein 7,Atg7)表达的影响。【方法】将40只雄性SD大鼠随机分为正常对照组8只和饮食诱导肥胖(Diet-induced obesity,DIO)造模组32只,造模组给予高脂饲料饲养14周制备DIO大鼠肥胖模型,取造模成功的16只大鼠随机分为肥胖模型组和针刺干预组,每组各8只。针刺干预组选取中脘、足三里、天枢、三阴交穴进行治疗,每天1次,每周连续针刺5 d,共针刺干预4周。观察各组大鼠体质量的变化,Western blot法检测大鼠下丘脑Atg7蛋白质的表达。【结果】高脂喂养14周后肥胖模型组和针刺干预组大鼠体质量均明显高于正常对照组,差异有统计学意义(P 0.05)。电针干预4周后,针刺干预组大鼠体质量显著低于肥胖模型组(P 0.05)。肥胖模型组大鼠下丘脑Atg7蛋白质含量明显低于正常对照组(P 0.05),电针干预组大鼠下丘脑Atg7蛋白质含量高于肥胖模型组(P 0.05)。【结论】电针干预可减轻肥胖大鼠体质量,可能与调控下丘脑自噬水平变化有关。     

葡萄糖调节蛋白78 mRNA在高脂喂养大鼠肝脏中的表达和意义

目的 检测高脂饲料喂养大鼠的肝脏葡萄糖调节蛋白7S(GILP78)mR2qA的表达情况,探讨内质网应激与胰岛素抵抗之间的关系及GKP78在胰岛素抵抗发生机制中的作用.方法 采用高脂饲料喂养建立胰岛素抵抗大鼠模型,30只雄性Wistar大鼠被随机分为基础饮食喂养组(NC,n=15)和高脂饮食喂养组(HF,n=15),分别喂养10用后,用正常血糖-高血浆胰岛素钳夹技术进行评估.应用Real Time PCR方法检测大鼠肝脏中GRP78 mRNA的表达.结果 ①高脂饲料组大鼠的葡萄糖输注率明显低于基础饲料组[GIR60-120(0.90±0.15)vs(4.97±0.68)mg/(ks·min),P<0.01].②高脂饲料组肝脏GRP78 mRNA的表达明显高于基础饲料组[(1.13±0.50)vs(0.43±0.10,P<0.01].③肝脏GRP78 mRNA表达与内脏脂肪占体质量的百分比(r=0.52,P=0.019)、总胆固醇(TC)(r=0.51,P=0.022)成正相关.结论 高脂诱导的胰岛素抵抗大鼠肝脏中GILP78mRNA表达增加,表明大鼠肝脏发生内质网应激和未折叠蛋白反应.     

高脂高糖饮食诱导的非酒精性脂肪性肝炎大鼠肝组织TLR4和MyD88 mRNA的表达

目的探讨Toll样受体4(TLR4)、髓样分化因子88(MyD88)在高脂高糖饮食诱导的非酒精性脂肪性肝病(NAFLD)大鼠肝组织中的表达及其意义。方法选取SD大鼠20只,随机分为两组,模型组SD大鼠给予高脂高糖饲料喂养,正常组给予普通饲料喂养,10周末分别进行如下检测:1计算肝指数;2HE染色,光镜下观察各组大鼠肝组织的病理改变;3应用RT-PCR法检测大鼠肝组织TLR4、MyD88 mRNA的表达。结果模型组的肝指数较正常组明显增加(P0.01);模型组的NAFLD活动度计分与正常组比较差异有统计学意义(P0.01);模型组大鼠肝组织TLR4和MyD88的mRNA表达水平与正常组比较差异有统计学意义(P0.01)。结论持续10周的高脂高糖饮食喂养可以诱导NAFLD大鼠模型,模型组大鼠肝组织TLR4 mRNA和MyD88 mRNA表达升高,在肝细胞炎症改变中起重要作用。     

高脂饮食诱导大鼠肥胖易感性差异的研究

目的　探讨高脂饮食诱导雌性SD大鼠肥胖易感性的差异及原因。方法　36 只雌性 SD大鼠按体重随机分为高脂实验组和基础对照组, 分别给予高脂饲料和基础饲料 13 周。实验结束时, 根据体重将高脂实验组大鼠分为饮食诱导肥胖 (DIO) 和饮食诱导肥胖抵抗 (DIO R) 大鼠, 比较各组相关指标的差异。结果　DIO大鼠体重、体长、Lee指数、脂肪湿重、脂体比、脏体比、热能摄入量、能量利用率等指标与对照组和DIO R大鼠相比均有显著性差异(均P<0 .05), DIO R大鼠与对照组相比均无显著性差异 (均P>0 .05)。结论　①含热量 19. 33 kJ/g的高脂饲料对雌性SD大鼠有致肥胖作用。②同种系、同性别大鼠对高脂饮食诱导的肥胖易感性存在明显的个体差异, 这种差异与热能摄入量和能量利用率等因素密切相关。     

INFLUENCE OF DIFFERENT DIET ON NPY EXPRESSION IN ARCUATE NUCLEUS OF RATS HYPOTHALAMUS

目的:研究不同膳食对大鼠下丘脑弓状核神经肽Y(NPY)表达的影响.方法:以高能饲料饲养大鼠建立膳食诱导肥胖(DIO)大鼠模型,采用SP免疫组织化学染色法观察大鼠下丘脑弓状核NPY蛋白的表达.结果:DIO组大鼠、饥饿组大鼠下丘脑弓状核NPY蛋白的表达明显高于对照组大鼠(P＜0.01,P＜0.05);DIO组大鼠与饥饿组大鼠比较,下丘脑弓状核NPY蛋白的表达无明显差别(P＞0.05).结论:禁食和高能饲料喂养均可上调大鼠下丘脑弓状核NPY的表达.     

S100A16在饮食诱导肥胖大鼠中的作用研究

目的:探讨S100A16蛋白对体重增加过程的影响,进一步研究S100A16蛋白在肥胖及肥胖相关疾病的发生发展过程中的作用?方法:正常8周龄大鼠随机分为正常饮食组(NF,n = 10)和高脂饮食组(HF,n = 10),建立饮食诱导的肥胖(DIO)大鼠模型,喂食14周时进行腹腔葡萄糖耐量试验(IPGTT)及胰岛素释放试验(IRT),喂食16周处死后称量皮下及内脏脂肪重量,采用HE染色方法 (hematoxylin and eosin staining,HE) 观察肝脏脂肪变性程度,放射免疫分析法检测血糖?血清胰岛素?尿酸等血清生化指标,同时应用Western blot方法检测脂肪组织中 S100A16及糖脂代谢相关转录因子的蛋白表达?结果:DIO组大鼠的体重?内脏脂肪明显高于正常组,血清总胆固醇和尿酸DIO组高于正常组但糖耐量和胰岛素释放低于正常组,Western blot显示DIO组大鼠脂肪和肝脏组织中S100A16?PPAR-γ?C/EBP-α的蛋白表达均高于正常组?结论:高脂饮食可上调S100A16及相关转录因子的表达;S100A16的高表达可促进脂质生成及肥胖发生,并对机体的胰岛素敏感性产生负性影响?     

11β-羟化类固醇脱氢酶1在饮食诱导性肥胖大鼠组织中的表达

目的：探讨11β-hydroxysteroid dehydrogenase type1（11β-HSD1）在肥胖发生过程中的作用。方法：建立饮食诱导的肥胖（DIO）大鼠模型，应用Westem blotting方法检测各组织11β-HSD1的蛋白表达，同时应用实时定量PCR检测LPL、resistin、FAS等肥胖相关基因的表达，采用放射免疫分析法检测血糖、血脂、胰岛素、TNFα等指标。结果：DIO组大鼠体重、体脂明显高于正常组，DIO组大鼠脂肪、脑、骨骼肌的11β-HSD1表达明显高于正常组，肥胖相关基因LPL、resistin、FAS等在DIO组内脏脂肪的表达高于正常组，外周血脂、胰岛素DIO组高于正常组，但血糖、TNF-α两组间无明显差异。结论：11β-HSD1在饮食诱导性肥胖大鼠相关组织的表达高于正常组，具有组织特异性；11β-HSD1在内脏脂肪组织的高表达促进肥胖的发生。     

脓毒症大鼠肝组织高迁移率族蛋白B1和Toll样受体4基因的表达及相关性

目的 探讨脓毒症大鼠肝组织高迁移率族蛋白B1(HMGB1)和Toll样受体4(TLR4)mRNA的表达变化及其相互关系.方法 采用盲肠结扎穿孔术(CLP)制成脓毒症大鼠模型.将50只Wistar大鼠分成正常对照组(10只)和CLP组(40只),CLP组分别于CLP术后6、12、18、24 h各处死10只大鼠,应用实时聚合酶链反应检测肝脏组织HMGB1和TLR4 mRNA的表达.结果 正常对照组大鼠肝脏组织存在微量的HMGB1、TLR4 mRNA表达.脓毒症组大鼠CLP术后各时间点的HMGB1、TLR4 mRNA表达均显著高于正常对照组(P值均<0.01),CPL术后6 h HMGB1、TLR4 mRNA表达开始升高,至12 h达峰值,18 h后开始下降,各时间点间HMGB1、TLR4 mRNA表达的差异均有统计学意义(P值均<0.05).直线相关分析表明,脓毒症大鼠肝脏组织HMGB1 mRNA与TLR4 mRNA的表达呈正相关(r=0.90,P=0.04).结论 HMGB1可能通过TLR4进一步激活炎性细胞,引起下游炎性因子释放的瀑布效应.     

不同膳食对大鼠下丘脑弓状核NPY表达的影响

目的:研究不同膳食对大鼠下丘脑弓状核神经肽Y(NPY)表达的影响。方法:以高能饲料饲养大鼠建立膳食诱导肥胖(DIO)大鼠模型,采用SP免疫组织化学染色法观察大鼠下丘脑弓状核NPY蛋白的表达。结果:DIO组大鼠、饥饿组大鼠下丘脑弓状核NPY蛋白的表达明显高于对照组大鼠(P<0.01,P<0.05);DIO组大鼠与饥饿组大鼠比较,下丘脑弓状核NPY蛋白的表达无明显差别(P>0.05)。结论:禁食和高能饲料喂养均可上调大鼠下丘脑弓状核NPY的表达。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.