| G-CSF-pJGW1/pGP1-2/DH5α工程菌的系统稳定性研究 |
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| 引用本文： | 黄树其,陈玉涛,齐锡位,陈德元,黄美华,王小宁.G-CSF-pJGW1/pGP1-2/DH5α工程菌的系统稳定性研究[J].第一军医大学分校学报,1997(2). |
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| 作者姓名： | 黄树其 陈玉涛 齐锡位 陈德元 黄美华 王小宁 |
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| 作者单位： | 解放军广州医学高等专科学校医学检验教研室 广州510315(黄树其，陈玉涛，齐锡位，陈德元，黄美华)，第一军医大学分子免疫学研究所 广州510515(王小宁) |
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| 摘 要： | 将构建好的rhG-CSF工程菌(G-CSF-PJGWI/pCP1-2/DH5a)由-70℃冰箱取出,连续培养140代,每20代观察工程菌质粒的遗传稳定性及rhG-CSF工程菌蛋白表达稳定性。结果表明:rhG-CSF工程菌在连续培养140代后,其抗生素抗性无改变,质粒DNA酶切鉴定与原始菌相同,其蛋白表达率与原始菌基本一致。
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| 关 键 词： | rhG-CSF pJGW1/pGP1 稳定性 工程菌 |
Study of the stability of recombinant human G-CSF expression system |
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| Huang Shuqi,Chen Yutao,Qi Xiwei,et al.Study of the stability of recombinant human G-CSF expression system[J].Journal of Branch Campus of the First Military Medical University,1997(2). |
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| Authors: | Huang Shuqi Chen Yutao Qi Xiwei |
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| Abstract: | The constructed engineering bacteria E.coli DH5a that contain plasmid pJGWl-hGCSF and pGPl-2 were cultured up to 140 generations. The antibiotics resistance and expressed protein level of rhG-CSF of the engineering bacteria is very stable. The recomhinant plasmids of pJGWl-hGCSF and pGPl-2 of different generations also showed the same electrophoretic patterns after restriction enzyme cleavage as those of the primary recombinant bacteria. |
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| Keywords: | recombinant hG-CSF pJGWl/pGPl stability engineering bacteria |
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