脱氧核糖核酸(DNA)中鸟嘌呤和腺嘌呤阳极差分脉冲伏安法同时测定

本文采用阳极差分脉冲伏安法同时测定微量DNA水解碱基鸟嘌呤和腺嘌呤的含量,从而建立了一种能对微量DNA进行快速测定G+C/A+T值的方法。本方法比层析分离后紫外吸收法灵敏度约高二个数量级,测定时间缩短到一小时半左右。经用德国小牛胸腺标准DNA进行实验,证明本方法准确可靠;并测定了大肠杆菌、棉铃虫等六种DNA的G+C/A+T值。

SIMULTANEOUS ANODIC DIFFERENTIAL PULSE VOLTAMMETRIC DETERMINATION OF GUANINE AND ADENINE OF DEOXYRIBONUELEIC ACID (DNA)

This paper reports that the micro amount of guanine and adenine in DNA can be determined simultaneously by anodic differential pulse voltam-metric method and so a rapid determination of G + C/A + T value of DNA is established. The sensitivity of this method is about 100 times higher than the ultra-violet adsorption method, in which the purines are seperated by paper chromotography. The time required for once determination is only1(1/2)hr.We use the DNA of calfthmos as the standard sample in the experiment and the result shows that this method is accurate. The G + C/A + T values of six sorts of DNA of E. coli, heliothis armigera and so on are determined.