| 基于脱乙酰基魔芋葡甘聚糖固定酶的葡萄糖传感器 |
| |
| 引用本文： | 郭晓明,王根礼,周祖新,朱贤,王荣,吴霞琴.基于脱乙酰基魔芋葡甘聚糖固定酶的葡萄糖传感器[J].分析试验室,2007,26(10):100-103. |
| |
| 作者姓名： | 郭晓明 王根礼 周祖新 朱贤 王荣 吴霞琴 |
| |
| 作者单位： | 1. 上海应用技术学院化工系,上海,200235
2. 上海师范大学化学系,上海,200234 |
| |
| 基金项目： | 上海应用技术学院校科研和教改项目 |
| |
| 摘 要： | 制备了脱乙酰基魔芋葡甘聚糖(d-KGM)的溶胶-凝胶,用红外光谱表征了其脱乙酰基前后的结构转化.探讨了d-KGM溶胶-凝胶的制备条件对其成膜性能及酶固定化的影响.在此基础上将d-KGM用于电极表面葡萄糖氧化酶的固定,制备了相应的葡萄糖传感器,并对传感器的工作条件进行了优化.所制备的传感器灵敏度为240 nA/mmol/L,线性范围为0.1～8 mmol/L,表观米氏常数KM为19.6 mmol/L,稳定性好,寿命长.实验结果表明d-KGM是一种可用于生物传感器中酶固定化的优良材料.
|
| 关 键 词： | 魔芋葡甘聚糖 脱乙酰基魔芋葡甘聚糖 溶胶-凝胶 酶固定化 葡萄糖传感器 |
| 文章编号： | 1000-0720（2007）10-100-04 |
| 修稿时间： | 2006-09-27 |
Glucose sensor based on immobilizing enzyme with deacetyled konjac glucomannan |
| |
| GUO Xiao-ming,WANG Gen-li,ZHOU Zu-xin,ZHU Xian,WANG Rong,WU Xia-qin.Glucose sensor based on immobilizing enzyme with deacetyled konjac glucomannan[J].Chinese Journal of Analysis Laboratory,2007,26(10):100-103. |
| |
| Authors: | GUO Xiao-ming WANG Gen-li ZHOU Zu-xin ZHU Xian WANG Rong WU Xia-qin |
| |
| Affiliation: | 1. Dept. of Chemistry and Engineering, Shanghai institute of Technology, Shanghai 200235; 2. Dept. of Chemistry, Shanghai Normal University, Shanghai 200234 |
| |
| Abstract: | The deacetyled Konjac glucomannan(d-KGM) sol-gel was prepared and the structural transformation from KGM to d-KGM was confirmed by FT-IR.The effects of experimental conditions related to the preparation of d-KGM sol-gel upon the properties of d-KGM membrane and the immobilization of enzyme were investigated.Glucose biosensor had been fabricated by immobilizing enzyme with d-KGM.The linear range of biosensor was 0.1 to 8 mmol/L with a sensitivity of 240 nA/mmol/L. The apparent Michealis-Menten constant is 19.6 mmol/L.Meanwhile,the biosensor showed good stability and reproducibility for long-term use.D-KGM is a promising material for immobilizing enzyme in biosensors. |
| |
| Keywords: | Konjac glucomannan Deacetyled konjac glucomannan Sol-gel Immobilization of enzyme Glucose sensor |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
