| 乌骨鸡肌肉中肌肽的鉴定与测定 |
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| 引用本文： | 田颖刚,谢明勇,王维亚,吴红静.乌骨鸡肌肉中肌肽的鉴定与测定[J].分析试验室,2007,26(1):5-8. |
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| 作者姓名： | 田颖刚 谢明勇 王维亚 吴红静 |
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| 作者单位： | 南昌大学食品科学教育部重点实验室,南昌,330047 |
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| 摘 要： | 肌肽是具有抗氧化、抗衰老等多种活性的功能性二肽. 建立了一种高效液相色谱测定鸡肌肉中肌肽的新方法, 该法采用NH2柱分离, 样品无需衍生, 操作简便. 通过该法和HPLC-MS 联用技术鉴定了乌骨鸡肌肉中存在肌肽, 同时比较乌骨鸡和普通白洛克鸡肌肉中肌肽的量. 结果表明: 该法适合于肌肉中肌肽的测定, 测定结果显示, 乌骨鸡肌肉中肌肽量约0.45%, 普通白洛克鸡肌肉中约0.22%. 乌骨鸡肌肉中肌肽量显著高于普通白洛克鸡, 这表明肌肽可能是体现乌骨鸡滋补营养作用的主要成分之一.
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| 关 键 词： | 乌骨鸡 肌肽 鉴定 含量测定 HPLC HPLC-MS联用 |
| 文章编号： | 1000-0720（2007）01-005-04 |
| 修稿时间： | 04 11 2006 12:00AM |
Identification and determination of Carnosine from muscles of Black-bone silky fowl |
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| TIAN Ying-gang,XIE Ming-yong,WANG Wei-ya,WU Hong-jing.Identification and determination of Carnosine from muscles of Black-bone silky fowl[J].Chinese Journal of Analysis Laboratory,2007,26(1):5-8. |
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| Authors: | TIAN Ying-gang XIE Ming-yong WANG Wei-ya WU Hong-jing |
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| Affiliation: | Key laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047 |
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| Abstract: | Carnosine is a functional dipeptide which naturally occurs in vertebrate animal tissues.In present work,a efficient method for the content determination of carnasine in muscles of chickens by HPLC was proposed,using a NH_2 column and no need for sample derivatization.Carnosine from muscles of Blackbone silky fowl was identified by combined use of HPLC/PAD/MS.Both contents of Carnosine from Black-bone silky fowl and White plymouth rock were determined by HPLC,the results showed that the content of Carnosine was much higher in the muscles of Black-bone silky fowl than that in White plymouth rock with 0.45% versus 0.22%.In this case,Carnosine is proposed to be one of the typical nutritional ingredients for the physiological functions of Black-bone silky fowl. |
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| Keywords: | Black-bone silky fowl Carnosine Identification Content determination HPLC HPLC/MS |
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