基于脾脏代谢组学方法研究低聚硒化氨基多糖对黑鲷的免疫调节作用

以冷冻甲醇提取,C18色谱柱和HILIC色谱柱分别分离黑鲷脾脏中的内源性代谢物,采用基于超高效液相色谱-飞行时间质谱联用技术(UPLC-TOF-MS)的非靶向代谢组学研究方法,分析了黑鲷饲喂低聚硒化氨基多糖后脾脏中内源性代谢物的变化差异,揭示了低聚硒化氨基多糖调节黑鲷免疫功能的潜在机制。采用XCMS^plus软件结合高分辨二级质谱数据库处理质谱原始数据,筛选出潜在生物标志物,并通过Metabo Analyst 4.0网站分析相关代谢通路。结果表明,黑鲷饲喂低聚硒化氨基多糖后脾脏中的36个代谢物发生显著变化;低聚硒化氨基多糖可通过9条代谢通路增强黑鲷的免疫机能。该研究结果为阐明低聚硒化氨基多糖的免疫增强机制提供了科学依据。

Investigation on Immunomodulatory Effect of Low Molecular Weight Seleno aminopolysaccharide on Acathopagrus Schlegelii by Spleen Metabonomics Analysis

To investigate the potential immunomodulatory mechanism of low molecular weight seleno-aminopolysaccharide,an untargeted metabonomics method based on ultra high performance liquid chromatography-time-of-flight mass spectrometry(UPLC-TOF-MS)was developed for analysis of the variations of spleen endogenous metabolites in Acathopagrus schlegelii after they were extracted with frozen methanol,then isolated with a C18 column and a HILIC column,respectively.The potential biomarkers were screened out by non-targeted mass spectrometry with XCMSplus software and Metabolite HR MS2 library,and the related metabolic pathways were analyzed by Metabo Analyst 4.0 website.Results showed that the spleen metabolites in the group fed with the low molecular weight seleno-aminopolysaccharide were significantly different from those in the blank group,and 36 biomarkers were found and identified.Low molecular weight seleno-aminopolysaccharide could enhance the immune function of Acathopagrus schlegelii through 9 metabolic pathways.This study provides a scientific basis for elucidating the immunoenhancement mechanism of low molecular weight seleno-aminopolysaccharide.