| 三齿多吡啶钴(II)配合物的合成、表征及其与DNA的相互作用研究 |
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| 引用本文： | 杨浩,包晓玉,行文茹,刘珊珊,陈文涛,邱东方.三齿多吡啶钴(II)配合物的合成、表征及其与DNA的相互作用研究[J].化学学报,2010,68(6):508-514. |
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| 作者姓名： | 杨浩 包晓玉 行文茹 刘珊珊 陈文涛 邱东方 |
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| 作者单位： | (南阳师范学院化学系 南阳 473061)(三门峡职业技术学院 三门峡 472000) |
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| 摘 要： | 合成了两种三齿多吡啶钴(II)配合物 (A)和Co(H2Bzimpy)2]Cl2 (B), 用元素分析、IR对配合物的组成和结构进行了表征, 测定了配合物A的晶体结构. 用电子吸收光谱、荧光光谱、循环伏安法及凝胶电泳实验等方法研究了配合物与DNA的相互作用. 结果表明配合物A和B与小牛胸腺(CTDNA)的作用属部分插入和静电结合, 凝胶电泳实验表明配合物A在310 nm光辐射15 min, 可使超螺旋pBR322DNA断裂为开环缺口型和线型DNA.
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| 关 键 词： | 三齿多吡啶配体 钴(II)配合物 晶体结构 DNA 光裂解 |
| 收稿时间： | 2009-07-17 |
| 修稿时间： | 2009-09-17 |
Synthesis, Characterization and DNA Interaction of Tridentate Polypyridyl Cobalt(II) Complexes |
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| Affiliation: | ( Department of Chemistry, Nanyang Normal University, Nanyang 473061)( Sanmenxia Polytechnic College, Sanmenxia 472000) |
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| Abstract: | Two tridentate polypyridyl complexes of cobalt(II) DMPhTPY＝6,6 -dimethyl-4 -phenyl-2,2 :6 ,2 -terpyridine] (A) and Co(H2Bzimpy)2]Cl2 H2Bzimpy]2＝2,6-bis(benz- imidazol-2-yl)pyridine] (B) were synthesized and characterized by elemental analysis and IR. The crystal structure of complex A was determined. The interactions of the complexes with calf thymus DNA (CTDNA) have been investigated by absorption spectroscopy, fluorescence spectroscopy, cyclic voltammetry and agarose gel electrophoresis. The studies suggested that complexes A and B bind to CTDNA by partial intercalation and electrostatic interaction. The agarose gel electrophoresis showed that complex A converted supercoiled pBR322 DNA to nicked and linear DNA when it was irradiated at 310 nm for 15 min. |
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| Keywords: | tridentate polypyridyl ligand cobalt(II) complex crystal structure DNA photocleavage |
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