| 金属离子对一种分子内电荷转移荧光探针与牛血清白蛋白相互作用的影响 |
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| 引用本文： | 刘毓芳,李建晴,董川.金属离子对一种分子内电荷转移荧光探针与牛血清白蛋白相互作用的影响[J].分析科学学报,2009,25(6). |
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| 作者姓名： | 刘毓芳 李建晴 董川 |
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| 作者单位： | 1. 晋中学院化学化工学院,山西晋中,030600;山西大学环境科学与工程研究中心,山西太原,030006
2. 山西大学环境科学与工程研究中心,山西太原,030006 |
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| 基金项目： | 国家自然科学基金，山西省自然科学基金 |
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| 摘 要： | 采用荧光光谱法研究了Fe~(3+)、Cu~(2+)、Pb~(2+)三种离子对1-酮-2-(对二甲氨基苯亚甲基)-四氢萘与牛血清白蛋白相互作用的影响.三种金属离子分别存在时能增强1-酮-2-(对二甲氨基苯亚甲基)-四氢萘对牛血清白蛋白的猝灭作用及二者的结合作用,使体系的猝灭常数及结合常数增大,且影响顺序为Fe~(3+)>Cu~(2+)>Pb~(2+).实验表明金属离子对蛋白质在物质的贮存、运转、代谢等方面有重要意义.
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| 关 键 词： | 金属离子 1-酮-2-(对二甲氨基苯亚甲基)-四氢萘 牛血清白蛋白 |
Effects of Metal Ions on the Interaction between an Intramolecular Charge Transfer Fluorescence Probe and Bovine Serum Albumin |
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| LIU Yu-fang,LI Jian-qing,DONG Chuan.Effects of Metal Ions on the Interaction between an Intramolecular Charge Transfer Fluorescence Probe and Bovine Serum Albumin[J].Journal of Analytical Science,2009,25(6). |
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| Authors: | LIU Yu-fang LI Jian-qing DONG Chuan |
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| Affiliation: | LIU Yu-fang1,2,LI Jian-qing1,DONG Chuan*2(1.College of Chemistry and Chemical Engineering,Jinzhong University,Jinzhong 0306002.Environmental-science and Engineering-study Center,Shanxi University,Taiyuan 030006) |
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| Abstract: | The influence of three ions,Fe3+,Cu2+ and Pb2+ on the interaction between 1-keto-2-(p-dimethylaminobenzal)-tetrohydronaphthalene(KDTN) and bovine serum album(BSA)was investigated using fluorescence spectometry.The quenching effects of KDTN on BSA and the binding affinity of the two compounds were enhanced in the presence of these three metal ions,respectively.The quenching constants and binding constants were all increased,and the impacts of these three ions on quenching and binding were in the order of Fe3... |
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| Keywords: | Metal ions 1-keto-2-(p-dimethylaminobenzal)-tetrohydronaphthalene Bovine serum albumin |
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