拉曼光谱-D401树脂吸附技术检测香根草根部重金属铅含量

以香根草作为研究材料，选取江铜贵冶周边土壤修复示范基地的香根草植株，利用其能富集重金属铅的特性，运用拉曼光谱结合树脂吸附技术检测Pb的含量。D401树脂功能基-N(CH₂COOH)₂中的N和O原子与金属离子发生配位能形成稳定化合物，从而富集香根草消煮溶液中的重金属Pb2+，然后用重金属分析仪(HM-5000P)测定铅含量。D401树脂和金属离子所形成的络合物有一定的拉曼信号，检测络合物的拉曼信号，运用拉曼光谱技术，对吸附重金属后树脂内Pb含量进行间接定量分析，建立数学检测模型。对比不同预处理方法对数据定量模型的影响，使用多种预处理方法结合偏最小二乘法建立最优的香根草根部重金属铅含量的定量分析模型。经过卷积平滑结合一阶微分预处理后其预测相关决定系数R_p为0.854，预测均方根误差RMSEP为5.658%，建模结果较理想。研究表明，基于拉曼光谱技术结合D401树脂吸附技术定量检测香根草根部重金属铅含量具有可行性，该研究对环境中重金属含量评估有一定的指导意义。

Detection of Heavy Metal Lead in Vetiver Grass Roots Combined Raman Spectroscopy with D401 Resin Adsorption Technology

In this paper,vetiver was selected as reserch material.It was taken from soil remediation semonstration base around Jiangxi Copper Guixi Smelter.It can enrich heavy metals.It put forward a detection method of measuring heavy metal lead in the vetiver grass roots using Raman spectroscopy combined with resin absorption in this paper.Nitrogen atom and Oxygen atom in functional groups of D401 resin can form a stable compound with metal ion.Firstly,vetiver enriched solution of heavy metals lead ion,then it was determined with HM-5000P Multi-function portable heavy metal analyzer.The complexion after functional groups in the D401 resin absorbed by heavy metals in plant have certain Raman spectrum information.Therefore,it can be used for indirect detect analysis the amount of heavy metals enriched in plant.In this paper,it proposed a detection method of measuring heavy metal lead in the vetiver grass roots using Raman spectroscopy combined with resin absorption.Partial least squares (PLS) combined with different spectral preprocessing methods was adapt to establish the quantitative analysis model of heavy metal lead in the vetiver grass roots department.After combined S.G.smoothing with first order differential pretreatment the optimal model got with the result that rp and RMSEP reached 0.854 and 5.658% respectively.The research showed the feasibility to detect heavy metal lead in the vetiver grass roots department combined Raman spectroscopy and D401 resin adsorption technique.This study examined the Pb of vetiver grass,which has great significance for the content of heavy metals in environmental assessment.