| 应用层流柜饲养实验性糖尿病Scid小鼠体会 |
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| 引用本文： | 李鸿,陈旺.应用层流柜饲养实验性糖尿病Scid小鼠体会[J].实验动物科学,2005,22(4):54-55. |
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| 作者姓名： | 李鸿 陈旺 |
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| 作者单位： | 中日友好临床医学研究所动物实验室,北京,100029 |
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| 摘 要： | Scid小鼠缺乏T和B淋巴细胞免疫功能,实验性糖尿病又进一步增加其感染机会,饲养难度更大。应用层流柜划分饲养及工作区,并设立“污染手制度”,在清洁环境内局部控制为SPF环境;日常饲养中适量添加维生素,成功饲养实验性糖尿病(Sevore Combined Immunodeficience Scid)小鼠四批,最长9周,顺利完成实验。实践证明,通过严密的环境控制,严格的操作程序,在清洁级环境中应用层流柜局部控制为SPF环境,短期饲养实验性糖尿病Scid小鼠是可行的。
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| 关 键 词： | Scid小鼠 实验性糖尿病 层流柜 动物实验 |
| 文章编号： | 1006-6179（2005）04-0054-02 |
| 收稿时间： | 2005-03-20 |
| 修稿时间： | 2005年3月20日 |
Breeding Diabetes Scid Mice with Streamline Cabinet |
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| LI Hong,CHEN Wang.Breeding Diabetes Scid Mice with Streamline Cabinet[J].Shiyan Dongwu Kexue,2005,22(4):54-55. |
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| Authors: | LI Hong CHEN Wang |
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| Abstract: | It was very difficulty breeding diabetes Scid mice for shortage of T and B lymphocyte and the animal was easy to get infection.This experiment had bred diabetes Scid mice successfully in the SPF region in streamline cabinet by measures such as:dividing breeding region and working region in streamline cabinet,dividing contaminate and clear hands,adding vitamins.It was practicable that breeding diabetes Scid mice in the SPF region in streamline cabinet short-term by accurate enviroment control and strict operating rules. |
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| Keywords: | Scid mice Scid Streamline cabinet Animal experience |
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