人输卵管积液对小鼠胚胎基质金属蛋白酶分泌的影响

目的探讨人输卵管积液对小鼠胚胎体外培养胚胎发育及着床能力的影响机制。方法收集人输卵管积液,小鼠促超排卵,自然受精后适时收集囊胚,并将其随机分配到含有不同浓度的输卵管积液的培养液中,进行体外培养,观察囊胚的发育,计算囊胚孵出率;采用逆转录聚合酶链反应及免疫印迹法测定囊胚和培养液中的基质金属蛋白酶9(MMP-9)和基质金属蛋白酶组织抑制因子1(TIMP-1)的含量。结果输卵管积液组的囊胚孵出率低于无积液组,囊胚及培养液中的MMP-9和TIMP-1低于无积液组,并呈剂量依赖性,MMP-9/TIMP-1随积液浓度增加而降低。结论输卵管积液通过降低胚胎MMP-9/TIMP-1的分泌和影响胚胎早期发育潜能,从而影响胚胎着床的能力。

Effect of human hydrosalpinx fluid on secretion of matrix metalloproteinases in mouse blastocysts

Objective: To determine how hydrosalpinx fluid affects matrix metalloproteinases.Methods: Human hydrosalpinx fluid was collected from the patients.Mouse blastocysts were collected from the superovulated mice following natural fertilization and cultured in vitro with different concentrations of hydrosalpinx fluid.The development of blastocysts was observed and the hatching rate calculated.The concentrations of MMP-9 and TIMP-1 in the blastocysts and in the culture medium were measured with RT-PCR and western blot.Results: Hydrosalpinx fluid significantly decreased the hatching rate of blastocysts and the concentrations of MMP-9 and TIMP-1 both in blastocysts and culture medium,in a dose dependent manner.Conclusion: Hydrosalpinx fluid might interfere with the blastocyst implantation by decreasing the MMP-9/TIMP-1 secretion and impairing the early development of blastocysts.