| 重组人白细胞介素－2在离子色谱分离时的复性 |
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| 引用本文： | 唐治华,罗丽华,虞冠华,施凤霞,丁树标,许祥裕.重组人白细胞介素－2在离子色谱分离时的复性[J].中国生物化学与分子生物学报,1996,12(3):341-343. |
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| 作者姓名： | 唐治华 罗丽华 虞冠华 施凤霞 丁树标 许祥裕 |
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| 作者单位： | 南京军区军事医学研究所 |
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| 摘 要： | 采用ＨＰＩＥＣ应用ＣＭ交换柱分离纯化Ｅ．ｃｏｌｉ工程菌表达的ｒＩＬ－２，与空气氧化复性相比较，又有１倍以上的变性ｒＩＬ－２在色谱过程中得到复性，且溶液ｐＨ影响ｒＩＬ－２的复性和纯化效果：ｐＨ７．０时ｒＩＬ－２复性率高于ｐＨ８．０时，但纯度明显低于ｐＨ８．０时分离的ｒＩＬ－２．
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| 关 键 词： | 白细胞介素－2 色谱法 蛋白质纯化 |
| 收稿时间： | 1996-06-20 |
Renaturation of Recombinant Interleukin-2 During Purification by Ion Exchange Chromatography |
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| Tang Zhi-Hua,Luo Li-Hua,Yu Guan-Hua,Shi Feng-Xia,Ding Shu-Biao,Xu Xiang-Yu.Renaturation of Recombinant Interleukin-2 During Purification by Ion Exchange Chromatography[J].Chinese Journal of Biochemistry and Molecular Biology,1996,12(3):341-343. |
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| Authors: | Tang Zhi-Hua Luo Li-Hua Yu Guan-Hua Shi Feng-Xia Ding Shu-Biao Xu Xiang-Yu |
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| Affiliation: | (Military medical Institute of Nanjing Command, PLA, Manjing 210002 |
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| Abstract: | By using high pressure ion exchange chromatography (HPIEC) of CM exchanger to purify recombinant interleukin-2 (rIL-2) expressed by engineered E. coli,about twice more the amount of rIL-2 were renaturated during the chromatographic purification. The renaturation was affected by PH of the buffer,the renaturation rate of rIL-2 in PH7.0 buffer was higher than that in PH8.0,but the purity was lower than that in PH8.0. |
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| Keywords: | Interleukin-2 Chromatography Protein purification |
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