臭氧浓度升高对毛竹叶片光合色素和抗性生理的影响

为了解毛竹对温室气体浓度升高的生理响应,探索竹子应对气候变化的经营策略,运用开顶式同化箱(OTCs)开展了5个O₃浓度梯度(过滤大气CF、环境大气NF、50nl·L^-1、100nl·L^-1、150nl·L^-1对毛竹(Phyllostachys edulis)叶片光合色素、可溶性蛋白、脂质过氧化和抗氧化系统的影响研究。结果表明:随O₃浓度升高,毛竹叶片Chl和Car含量呈下降趋势,可溶性蛋白、MDA和O₂^-含量呈升高趋势。与CF相比,100nl·L^-1和150nl·L^-1O₃,浓度处理毛竹叶片光合色素含量极显著降低,叶片可溶性蛋白、MDA和O₂^-含量极显著升高;随O₃浓度升高,SOD活性呈降低趋势,100nl·L^-1和150nl·L^-1O₃浓度处理较CF极显著下降。POD活性呈升高趋势,100nl·L^-1和150nl·L^-1O₃浓度处理较CF极显著提高;长时间高O₃浓度(100nl·L^-1及以上)胁迫条件下,会导致毛竹叶片光合色素降解或合成受阻,叶片老化加快,膜脂过氧化程度加剧,膜结构和抗氧化系统功能遭到破坏,影响毛竹的正常生长。

Effects of elevated ozone concentration on photosynthetic pigment and resistance physiology in leaves of Phyilostachys edulis

In order to explore the management strategies of bamboo under the context of climate change,the effects of elevated ozone concentrations on photosynthetic pigments content,soluble protein content,lipid peroxidation and anti-oxidation system in the leaves of Phyllostachys edulis grown in open-top chambers(OTCs) were investigated.The results showed that with the ozone concentration increasing,the contents of the chlorophyll,carotenoid,and SOD activity decreased;while the contents of soluble protein,MDA,O₂^- and POD activity increased.Elevated ozone concentrations at 100 and 150 nl·L^-1 significantly decreased the photosynthetic pigments content and SOD activity;while increased soluble protein content,MDA content,O₂^- content and POD activity.The results suggested that the elongated ozone exposure inhibited the growth of P.edulis by restraining the synthesis or speeding the degradation of photosynthetic pigments,accelerating the aging of leaves,intensifying the damage to membrane lipid,and destroying the structure of cell membrane and the function of anti-oxidation system.