| 电喷雾多级串联质谱技术区分人乳寡糖异构体 |
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| 引用本文： | 韩瑶,吕志华,姜廷福,王远红. 电喷雾多级串联质谱技术区分人乳寡糖异构体[J]. 分析化学, 2006, 34(9): 1213-1218 |
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| 作者姓名： | 韩瑶 吕志华 姜廷福 王远红 |
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| 作者单位： | 中国海洋大学医药学院海洋药物与食品研究所,青岛,266003;中国海洋大学医药学院海洋药物与食品研究所,青岛,266003;中国海洋大学医药学院海洋药物与食品研究所,青岛,266003;中国海洋大学医药学院海洋药物与食品研究所,青岛,266003 |
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| 基金项目： | 国家973资助项目(No.2003CB7/6400) |
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| 摘 要： | 利用四极杆-飞行时间(Q-TOF)电喷雾多级串联质谱(ESI-MS/MS)与诱导碰撞解离技术(C ID)在负离子模式下选择合适碰撞能量对几组互为异构体的人乳寡糖进行了分析,并对其碎裂机理进行了探讨。结果表明:在二级质谱中碰撞能量范围为15~30 eV时,寡糖异构体产生其特征碎片离子,据此可以快速灵敏地区分寡糖异构体。
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| 关 键 词： | 电喷雾多级串联质谱 寡糖异构体 人乳寡糖 |
| 收稿时间： | 2005-12-25 |
| 修稿时间： | 2005-12-252006-04-05 |
Application of Electrospray Ionization-Collision Induced Dissociation-Tandem Mass Spectrometry in Differentiation Isomers of Human Milk Oligosaccharides |
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| Han Yao,Lü Zhihua,Jiang Tingfu,Wang Yuanhong. Application of Electrospray Ionization-Collision Induced Dissociation-Tandem Mass Spectrometry in Differentiation Isomers of Human Milk Oligosaccharides[J]. Chinese Journal of Analytical Chemistry, 2006, 34(9): 1213-1218 |
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| Authors: | Han Yao Lü Zhihua Jiang Tingfu Wang Yuanhong |
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| Affiliation: | Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China, Qingdao 266003 |
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| Abstract: | Electrospray ionization-collision induced dissociation-tandem mass spectrometry(ESI-CID-MS/MS) is becoming one of the most important tools for structure analysis of oligosaccharides.In this study,a combined ESI-CID-MS/MS method on quadrupole-time of flight(Q-TOF) was developed to differentiate several isomers in human milk oligosaccharides.Under MS/MS conditions,negative ion mode,changing the CID eneryg from 15 eV to 30 eV,some useful fragments were obtained and the fragmentation mechanisms were proposed.The experimental results demonstrate that isomers in human milk oligosaccharides can be distinguished by their characteristic fragment ions quickly and conveniently. |
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| Keywords: | Electrospray ionization-collision induced dissociation-tandem mass spectrometry oligosaccharide isomers human milk oligosaccharides |
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