依达拉奉对脓毒症大鼠心肌损伤的保护作用

目的  观察依达拉奉对脓毒症大鼠心肌损伤的保护作用。方法  78只清洁级SD大鼠随机分为正常组（Norm组）、假手术组（Sham组）、脓毒症组（CLP组）、小剂量依达拉奉治疗组（ED1组）（3 mg/kg，静脉注射）及大剂量依达拉奉治疗组（ED2组）（6 mg/kg，静脉注射）。盲肠结扎穿孔法制作脓毒症模型，ED1组及ED2组给予依达拉奉治疗。分别于术后3、6及12 h检测各组血清cTnI和心肌·OH-、丙二醛（MDA）、超氧化物歧化酶（SOD）、肿瘤坏死因子（TNF-α）及白细胞介素1β（IL-1β）水平，并观察心肌病理形态改变。结果  Norm组与Sham组各指标差异无统计学意义（P >0.05）；CLP组与Norm组及Sham组比较，cTnI、·OH-、MDA、TNF-α及IL-1β水平明显升高，SOD水平明显降低（P <0.01）；ED1组及ED2组与CLP组比较cTnI、·OH-、MDA、TNF-α及IL-1β水平明显降低，SOD水平明显升高（P <0.01）；ED2组与ED1组比较，cTnI、·OH-、MDA、TNF-α及IL-1β水平明显降低，SOD水平明显升高（P <0.01）。CLP组心肌病理学改变包括间质充血水肿，炎症细胞浸润，心肌纤维断裂、坏死；ED1组及ED2组心肌损伤较CLP组明显减轻。结论  ①依达拉奉能减轻脓毒症心肌损伤，机制可能与抑制氧化应激及下调炎症因子水平有关；②大剂量依达拉奉（6 mg/kg）对脓毒症心肌损伤的保护作用较小剂量（3 mg/kg）更明显。

Protective effect of edaravone on sepsis-related myocardial injury in rats

Objective To investigate the protective effect of edaravone on sepsis-related myocardial injury in rats. Methods Seventy eight healthy male Sprauge-Dawley (SD) rats were randomly divided into normal group (Norm), sham operation group (Sham), sepsis group (CLP), low-dose edaravone treated group (ED1) and high-dose edaravone treated group (ED2). A rat model of sepsis-related myocardial injury was developed by cecal ligation and puncture (CLP). Edaravone was administered to the rats in ED1 and ED2 group. Serum cardiac troponin I (cTnI) was measured, and hydroxyl radicals (OH-), malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were examined in the heart tissue at various time points (3 h, 6 h, and 12 h after operation). Myocardial pathomorphology was also observed. Results Changes of all indexes were not significantly different between Norm group and Sham group (P > 0.05). In ED1 and ED2 group, cTnI, OH-, MDA, TNF-α, and IL-1β were significantly higher than in Norm and Sham group, but lower than in CLP group; SOD was significantly lower than in Norm and Sham group, but higher than in CLP group (P < 0.01). In ED2 group, cTnI, OH-, MDA, TNF-α, and IL-1β were significantly lower, but SOD was significantly higher than in ED1 group (P < 0.01). Myocardial pathomorphology in CLP group included edema, congestion in mesenchyma, and inflammatory cells infiltration were not significantly changed in Norm group and Sham group, but alleviated evidently in ED1 group and ED2 group. Conclusions Edaravone alleviates sepsis-related myocardial injury, which probably related to the inhibition of oxidative stress and cytokines. High-dose edaravone (6 mg/kg) is more efficient than low-dose (3 mg/kg) in protective effect on sepsis-related myocardial injury.