| 茶树基因组DNA测试方法概述 |
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| 引用本文： | 谭和平,沈兴中,唐祥凯,叶德萍,王智. 茶树基因组DNA测试方法概述[J]. 中国测试技术, 2010, 36(1) |
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| 作者姓名： | 谭和平 沈兴中 唐祥凯 叶德萍 王智 |
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| 作者单位： | 中国测试技术研究院,四川,成都,610021 |
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| 基金项目： | 国家科技支撑计划重点项目(2008BAK41B01-7) |
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| 摘 要： | 在查阅国内外关于茶树DNA测试方法研究文献的基础上,结合茶树DNA测试方法的现状,对茶树基因组DNA的主要测试方法进行了归纳,指出了DNA的提取、定量及其分子标记三类测试方法研究中的主要进展,并对DNA提取过程中的主要影响因素、分子标记方法的优缺点进行了评述,提出对茶树中DNA测试方法实行标准化,是实现茶树的种质资源鉴定、优良品种选育的重要途径。
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| 关 键 词： | DNA提取 定量分析 分子标记 茶树 |
Overview of testing methods for genomic DNA from tea plant |
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| TAN He-ping,SHEN Xing-zhong,Tang Xiang-kai,YE De-ping,WANG Zhi. Overview of testing methods for genomic DNA from tea plant[J]. China Measurement Technology, 2010, 36(1) |
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| Authors: | TAN He-ping SHEN Xing-zhong Tang Xiang-kai YE De-ping WANG Zhi |
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| Affiliation: | TAN He-ping,SHEN Xing-zhong,Tang Xiang-kai,YE De-ping,WANG Zhi(National Institute of Measurement , Testing Technology,Chengdu 610021,China) |
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| Abstract: | Almost all literatures about the testing methods of genomic DNA from tea plant have been searched.This study summarizes the major testing methods of genomic DNA from tea plant,and points out the major advances in three types of test methods,i.e.DNA extraction,quantitative analysis and molecular marker studies.It also discusses the main factors affecting the DNA extraction process and the advantages and disadvantages of molecular marker methods.As the result,it was suggested that the standardization of testi... |
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| Keywords: | DNA extraction Quantitative analysis Molecular marker Tea plant |
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