| 重瓣榆叶梅PrtSHP的基因克隆与序列结构分析 |
| |
| 引用本文： | 刘志雄,于先泥.重瓣榆叶梅PrtSHP的基因克隆与序列结构分析[J].湖北农业科学,2012,51(10):2124-2127. |
| |
| 作者姓名： | 刘志雄 于先泥 |
| |
| 作者单位： | 长江大学园艺园林学院,湖北荆州,434025 |
| |
| 基金项目： | 国家自然科学基金项目(31101202);长江大学博士启动基金项目 |
| |
| 摘 要： | 采用同源克隆法结合3′-RACE技术从重瓣榆叶梅(Prunus triloba var.Plena)中分离得到了1个雌蕊和果实发育调控基因SHP的同源基因PrtSHP的全长cDNA(GenBank登录号:JF911701).其cDNA全长970 bp,包括1个编码244个氨基酸的735 bp的完整开放阅读框.蛋白质序列比对和分子系统发生分析表明,PrtSHP是拟南芥的SHP的同源基因,其编码的蛋白质的C末端结构域具有2个高度保守的模体(AG Ⅰ模体和AG Ⅱ模体),属C类转录因子中PLE进化系.
|
| 关 键 词： | 重瓣榆叶梅(Prunus triloba var.Plena) 雌蕊和果实发育 基因克隆 序列分析 |
Gene Clone and Sequence Analysis of PrtSHP from Prunus triloba var. Plena |
| |
| LIU Zhi-xiong
,
YU Xian-ni.Gene Clone and Sequence Analysis of PrtSHP from Prunus triloba var. Plena[J].Hubei Agricultural Sciences,2012,51(10):2124-2127. |
| |
| Authors: | LIU Zhi-xiong YU Xian-ni |
| |
| Affiliation: | (College of Horticulture and Gardening,Yangtze University,Jingzhou 434025,Hubei,China) |
| |
| Abstract: | The total cDNA sequence of PrtSHP(GenBank accession code,JF911701) of Prunus triloba var.Plena,the homologous gene of pistil and fruit developmentally regulated gene SHP,was obtained by homology cloning combined with 3′-RACE.The full length of PrtSHP cDNA was 970 bp,including an open reading frame(ORF) of 735 bp which code a polypeptide of 244 amino acids.Sequence comparison and phylogenetic analysis revealed that PrtSHP was highly homologous to protein SHP in Arabidopsis and belonged to the PLE clade of class C.The C-terminal domain of the predicted amino acid sequence of PrtSHP harbored two highly conserved motifs(AG Ⅰand AG Ⅱ). |
| |
| Keywords: | Prunus triloba var Plena pistil and fruit development gene clone sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
