姜黄素对中子照射后小鼠空肠上皮细胞增殖活力的影响

目的探讨姜黄素对中子照射后小鼠空肠上皮细胞增殖活力的影响。方法选取二级雄性BALB/c小鼠120只,随机分为对照组、3Gy中子照射组和3Gy中子照射+姜黄素治疗组。照射组和姜黄素治疗组小鼠采用3Gy中子全身均匀照射,姜黄素治疗组于照射后即日腹腔注射200mg.kg-1.d-1的姜黄素,每天1次,连续给药5 d。于照射后6 h、1 d、3 d和5 d活杀取材,留取小鼠空肠组织,采用AgNOR、Feulgen染色和图像分析等手段,定量检测小鼠空肠上皮细胞核内嗜银蛋白数密度和DNA含量积分光密度的变化。结果照射组和姜黄素组于照后6 h～5 d,空肠上皮细胞核内AgNOR和DNA含量明显下降(P<0.01);照后3～5 d姜黄素组小鼠空肠上皮细胞AgNOR和DNA含量较照射组有所增加(P<0.05或0.01),但相比对照组仍较低。结论 3Gy中子照射引起小鼠空肠上皮细胞增殖活力明显降低;应用姜黄素治疗可增加空肠上皮细胞的增殖活力,对中子辐射肠上皮损伤具有保护作用。

Effect of curcumin on the mouse intestinal cell proliferation activity after neutron irradiation

Objective To explore the effect of curcumin on the mouse intestinal cell proliferation activity after neutron irradiation.Methods One hundred and twenty male BALB/c mice were randomly divided into control group,3 Gy neutron irradiation group and 3 Gy neutron irradiation ＋ curcumin treatment group.The mice of irradiation group and curcumin treatment group were whole body exposed to 3 Gy neutron irradiation.The mice in curcumin treatment groups were injected curcumin（200 mg·kg-1·d-1） intraperitoneally once a day for 5 days after irradiation.The mice were killed and the jejunum were taken for histopathological examination.The contents of argyrophilic nucleaolar organizer regions（AgNOR） and DNA in the intestinal epithelial cells after neutron irradiation were observed using AgNOR staining,Feulgen staining and image analysis.Results The contents of AgNOR and DNA in the mice jejunum were decreased progressively at 6 h to 5 d after the 3 Gy neutron irradiation（P0.01）.Those of the curcumin treatment group were higher than that in the the irradiation group at 3 d to 5 d after the neutron irradiation（P0.05 or 0.01）,but still lower than those of the control group mice.Conclusions The mouse intestinal cell proliferation activity is seriously damaged by 3 Gy neutron irradiation.Curcumin can reduce the damage and promote the mouse intestinal cell proliferation activity.