皮肤病理性瘢痕和瘢痕癌中Ras基因家族12/13位密码子突变的检测

目的:探讨皮肤病理性瘢痕和瘢痕癌中Ras基因家族(H-ras/N-ras/K-ras)12/13位密码子的突变情况。方法:从14例皮肤病理性瘢痕,14例瘢痕癌石蜡包埋组织中提取DNA,进行PCR扩增及测序,分析H-ras、N-ras、K-ras基因第12、13位密码子突变情况。结果:28个样本均成功提取DNA,扩增出基因片段,经测序均未发现H-ras、N-ras、K-ras基因第12、13位密码子的点突变。结论:皮肤病理性瘢痕和瘢痕癌中Ras基因家族的突变位点有待进一步探讨。

Mutation of ras gene family codons 12 and 13 in skin pathological scar and scar carcinoma tissues

Objective To explore the mutation of Ras gene family(H-ras /N-ras /K-ras) in skin pathological scar and scar carcinoma tissues.Methods The DNA was extracted from the choosen samples fixed in 10% formalin and Paraffiembedded(including 14 cases skin pathological scar tissues and 14 cases skin scar carcinoma tissues),H-ras,N-ras and K-ras mutations in codons 12 and 13 were analyzed by PCR and sequencing.Results DNA was extracted from the choosen 28 samples.By PCR,the 28 samples were succeed to enlarge the gene piece.Without gene mutation in codons 12 and 13 of H-ras,N-ras and K-ras genes were examined by sequencing.Conclusions The mutation situs of Ras family is to be further explored in skin scar and scar carcinoma.