亚硒酸钠在SGC-7901细胞生长中的抑制作用

目的：研究亚硒酸钠对SGC-7901细胞生长的影响。方法：应用细胞培养和SRB实验探讨了亚硒酸钠对SGC-7901细胞生长曲线的影响：应用集落形成试验、分裂指数试验研究了亚硒酸钠对SGC-7901细胞集落形成和分裂指数的影响；应用流式细胞仪检测了亚硒酸钠对SGC-7901细胞生长周期的影响；应用电镜、TUNEL染色及流式细胞仪观察了亚硒酸钠诱导SGC-7901细胞凋亡的作用。结果：亚硒酸钠溶液对SGC-7901细胞的生长曲线、集落形成、分裂指数有明显的抑制作用，其抑制作用与其作用浓度和作用时间呈正相关。流式细胞仪检测显示亚硒酸钠作用SGC-7901细胞24h后，细胞周期发生改变，G1期细胞百分率减少，S期细胞百分率增加，与对照组相比有显著性差异（p〈0．05）：电镜下，细胞核固缩，染色质凝集呈新月形紧贴于核膜周边，核膜扭曲；DNA直方图上出现典型的亚二倍体的“凋亡峰”；TUNEL染色法检测细胞凋亡指数在10．4％～33．4％。结论：亚硒酸钠溶液对SGC-7901细胞的生长具有抑制作用，其抑制作用程度与其作用浓度和时间呈正相关：亚硒酸钠通过阻滞细胞S期抑制细胞增殖及诱导细胞凋亡可能是其抑制SGC-7901细胞生长的机理之一。

Inhibitory Effect of Sodium Selenite on the Growth of SGC-7901 Cells

Objective: To examine the effect of sodium selenite on growth of SGC-7901 cells. Methods: The growth curve of SGC-7901 cells was measured by using cell culture and SRB experiment; effects of sodium selenite on SGC-7901 cell colony- forming and cell mitotic index were examined by using the relative tests; effect of sodium selenite on SGC-7901 cell growth cycle was measured by using flow cytometry; and sodium selenite-induced SGC-7901 cell apoptosis was examined by electron microscope, TUNEL staining and flow cytometry. Results: In the curve sodium selenite significantly inhibited respectively the SGC-7901 cell growth, colony-forming and mitotic index dose dependently. Flow cytometry detection showed that, after 24 hours, sodium selenite reduced the percentage of G1 phase cells but increased the percentage of S phase cells in cell cycle (p< 0.05 compared with control group). Electron microscope detection showed nucleus pycnosis, and crescent-shaped chromatic agglutination stiching closed to karyotheca with karyotheca distortion. DNA histogram showed a typical apoptosis peak as hyodiplod. TUNEL staining showed that apoptosis index was between 10.4% and 33.4%. Conclusion: Sodium selenite had an inhibitory effect on SGC-7901 cell growth with dose-effect relationship. The inhibition of S phase cell proliferation and induction of cell apoptosis might underlie the mechanism of the inhibitory effect of sodium selenite on SGC-7901 cell growth.