| EGFR 抑制剂对舌鳞癌多药耐药细胞 Tca8113／CBP耐药蛋白的影响 |
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| 引用本文： | 刘得玺,窦志茜,李娟,张丽,倪小兵,牛玉明,罗志晓,冷卫东.EGFR 抑制剂对舌鳞癌多药耐药细胞 Tca8113／CBP耐药蛋白的影响[J].临床口腔医学杂志,2016(8):459-461. |
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| 作者姓名： | 刘得玺 窦志茜 李娟 张丽 倪小兵 牛玉明 罗志晓 冷卫东 |
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| 作者单位： | 1. 湖北医药学院附属十堰市太和医院口腔医学中心 湖北 十堰 442000;2. 湖北医药学院附属十堰市太和医院产科 湖北 十堰 442000 |
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| 基金项目： | 湖北省教育厅科研计划项目(D20122405) |
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| 摘 要： | 目的：探讨表皮生长因子受体（ EGFR）抑制剂在舌鳞癌多药耐药中的作用。方法：免疫细胞化学技术和Western blot方法检测舌癌细胞系Tca8113及多药耐药细胞系Tca8113／CBP EGFR的表达，Western blot检测EGFR受到抑制后舌癌多药耐药细胞Tca8113／CBP耐药蛋白MRP，GTS-π的表达。结果：细胞系Tca8113和Tca8113／CBP均有EGFR的表达（P＞0．05），EGFR受到抑制后细胞Tca8113／CBP的多药耐药蛋白MRP，GST-π的表达均下降（P＜0．05）。结论：抑制EGFR可下调舌癌多药耐药细胞耐药蛋白的表达。
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| 关 键 词： | EGFR 西妥昔 舌癌 多药耐药 Tca8113/CBP细胞 |
The effect of anti-EGFR agents on multidrug resistance in tongue squamous cell carcinoma drug-re-sistance cell Tca8113/CBP |
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| Abstract: | Objective:To study the effect of anti-EGFR agents on multidrug resistance( MDR) in tongue squamous cell carcinoma drug-resistance cell line Tca8113/CBP.Methods:Immunohistochemical staining and western blot assay were applied to detect EGFR expression in tongue squamous cell carcinoma cell line Tca8113 and its MDR cell line Tca8113/CBP, the protein expression levels of MRP, GST-πin cell line Tca8113/CBP which EGFR expression were inhibited were detected by western blot assay.Results:We found higher expression of EGFR in cell lineTca8113 and its MDR cell line Tca8113/CBP(P>0.05).After the EGFR expression were inhibited,the expression MRP and GTS-πwere significant decrease in MDR cell line Tca8113/CBP( P<0.05) . Conclusion:The anti--EGFR agents can lead to a downregulation of resistance protein expression. |
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| Keywords: | EGFR Cetuximab Tongue Squamous Cell Carcinoma Multidrug Resistance Cell Line Tca8113/CBP |
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