芬太尼对胃癌MGC-803细胞E2F-1、p53基因表达的影响

目的观察芬太尼对人胃癌MGC-803细胞生长增殖及E2F-1、p53基因表达的影响。方法芬太尼10-4μmol/L与胃癌MGC-803细胞共同孵育为芬太尼组,未加入芬太尼的胃癌MGC-803细胞孵育为对照组。7d后检测胃癌MGC-803细胞生长增殖的变化,观察细胞超微结构的变化,检测细胞E2F-1、p53基因的表达。结果与对照组相比,芬太尼组MGC-803细胞生长增殖缓慢,细胞克隆形成率更低(P<0.05);细胞胞质深染、细胞核固缩、染色质碎裂,出现明显的凋亡表现;细胞E2F-1基因表达增加、p53基因表达减少。结论 10-4μmol/L芬太尼可抑制胃癌MGC-803细胞的生长增殖,促进细胞的凋亡,并改变E2F-1、p53基因的表达。

Effects of fentanyl on the expression of E2F-1 and p53 genes of gastric carcinoma MGC-803 cells

Objective To observe the effects of fentanyl on the proliferation of human gastric carcinoma MGC-803 cells and the expression of E2F-1 and p53 genes.Methods MGC-803 cells were incubated with fentanyl 10-4 μmol/L (fentanyl group) or not (control group).Seven days later,the proliferation of MGC-803 cells was determined;the ultrastructure of MGC-803 cells was observed;and the expression of E2F-1 and p53 genes in MGC-803 cells was determined.Results Compared with control group,the proliferation of MGC-803 cells was slow with fewer cloning rate of MGC-803 cells in fentanyl group;morphological changes of apoptosis such as nuclear shrinkage and chromosomal DNA fragmentation were shown in fentanyl group;and E2F-1 mRNA upregulation and p53 mRNA downregulation were observed in fentanyl group.Conclusion Fentanyl 10-4 μmol/L may inhibit proliferation,promote apoptosis and alter the expression of E2F-1 and p53 genes of carcinoma MGC-803 cells.