An establishment of ELISA for murine IL-6 |
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| Authors: | Takashi Saito Kensuke Futatsugi Daisuke Miki Hiroshi Suzuki Kiyoshi Yasukawa |
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| Affiliation: | (1) Biotechnology Research Laboratory, Tosoh Corporation, 2743-1, 252 Ayase-shi, Kanagawa, Hayakawa, Japan |
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| Abstract: | Summary Murine interleukin-6 (mIL-6) was expressed inEscherichia coli as human growth hormone (hGH) fusion protein. The products were cleaved by thrombin to liberate mIL-6. Monoclonal and polyclonal antibodies specific to mIL-6 were prepared by immunizing rats with mIL-6 thus obtained. ELISA for the quantitation of mIL-6 was also established, which could detect mIL-6 in a quantity as low as 2 ng/ml. |
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