| 银杏叶提取物对羟自由基所致DNA损伤保护作用的研究 |
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| 引用本文： | 李建萍,左克强,张小清,喻泽兰.银杏叶提取物对羟自由基所致DNA损伤保护作用的研究[J].江苏大学学报(医学版),2005,15(1):34-35. |
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| 作者姓名： | 李建萍 左克强 张小清 喻泽兰 |
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| 作者单位： | 湘南学院基础医学课部,湖南,郴州,423000 |
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| 摘 要： | 目的:研究银杏叶提取物(Ginkgo-bilobaExtract,EGb)对羟自由基(·OH)所致DNA氧化损伤的保护作用。方法:采用生物化学发光技术,应用硫酸铜-邻菲罗啉-抗坏血酸-过氧化氢(CuSO4-phen-VitC-H2O2)化学发光体系中测定EGb清除·OH的数据,并通过发光抑制率分析EGb对DNA氧化损伤的保护作用。结果:EGb能显著降低发光体系的发光,而且EGb浓度愈大,发光抑制率愈大。结论:EGb具有较强的抗氧化作用,能保护DNA免受·OH的氧化损伤。
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| 关 键 词： | 银杏叶提取物 化学发光 DNA损伤 羟自由基 |
| 文章编号： | 1671-7783(2005)01-0034-02 |
| 修稿时间： | 2004年12月31 |
Protective Effect of Ginko-biloba Extract on the DNA Damage Induced by Hydroxyl Radical |
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| LI Jian-ping,ZUO Ke-qiang,Zhang Xiao-qin,YU Ze-lan.Protective Effect of Ginko-biloba Extract on the DNA Damage Induced by Hydroxyl Radical[J].Journal of Jiangsu University Medicine Edition,2005,15(1):34-35. |
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| Authors: | LI Jian-ping ZUO Ke-qiang Zhang Xiao-qin YU Ze-lan |
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| Abstract: | Objective: To study protective effect of EGb on DNA damage caused by hydroxyl radical. Methods: The hydroxyl radical were deleted by CuSO4-phen-VitC-H2O2 biochemiluminescence systems. And then analyzed the protective effect of EGb on DNA peroxidation damage by biochemiluminescence inhibit rate. Results: The ultra-weak chemiluminescence during DNA peroxidation induced by CuSO4-phen-VitC-H2O2-DNA were all inhibited by EGb. Conclusion: EGb possessed potent antioxidative activity and reduced peroxidation of DNA damaged by hydroxyl radicals. |
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| Keywords: | EGb Chemiluminescence method DNA Damage Hydroxyl radical |
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