淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究

[目的]比较淫羊藿水提物及水提后剩余部分(即药渣)对大鼠骨质疏松的治疗作用。[方法] 50只雌性SD大鼠行双侧卵巢摘除手术,术后12周随机分为5组,即淫羊藿水提物1 g/kg组(以生药计,下同)、水提物0.5 g/kg组、药渣1 g/kg组、药渣0.5 g/kg组和模型组。另10只SD大鼠作为对照组。各组大鼠连续灌胃给药8周后处死,取肝、肾、子宫称质量并计算脏器指数,取血进行血清生化指标检测,取胫骨应用Micro-CT进行骨微结构检测。[结果]给药8周后,各给药组血清骨钙素(OC)、I型胶原N端前肽(PINP)、I型前胶交联C末端肽(CTX1)与模型组相比少有显著性差异,但有向对照组正常水平恢复的趋势。谷丙转氨酶(ALT)、谷草转氨酶(AST)、血清总胆固醇(TC)、甘油三酯(TG)和葡萄糖(GLU)均有升高趋势。CT扫描结果显示给药组较模型组骨体积、骨面积、骨小梁数量显著升高、骨小梁厚度显著降低,且0.5 g/kg药渣组比0.5 g/kg水提物组骨微结构有显著提升(P<0.05)。[结论]淫羊藿水提物及药渣对去势所致的雌性大鼠骨质疏松有治疗作用,0.5 g/kg淫羊藿药渣的治疗效果优于同等剂量水提物。

Comparative study on anti-osteoporosis efficacy of epimedium water extract and its residue

[Objective] To compare the therapeutic effects of epimedium water extract and its residue after water extraction on osteoporosis in rats.[Methods] Fifty female SD rats underwent bilateral ovariectomy were randomly divided into five groups twelve weeks after operation:epimedium water extract 1 g/kg group (based on crude drugs,the same below),water extract 0.5 g/kg group,drug residue 1 g/kg group,drug residue 0.5 g/kg group and model group. Another ten SD rats were used as control group. Rats in each group were sacrificed after 8 weeks of continuous administration. Liver,kidney and uterus were weighed and organ index was calculated. Blood was taken for serum biochemical index detection,and tibia was taken for bone microstructure detection by Micro-CT.[Results] After 8 weeks of administration,the serum OC,PINP,CTX1 in each group had less significant difference compared with the model group,but had a tendency to recover to the normal level in the control group;ALT,AST,TC,TG and GLU had an increasing trend. CT scans showed that the bone volume,bone area,the number of bone trabeculae and the thickness of bone trabeculae in the drug group were significantly higher than those in the model group,and the bone microstructures in the 0.5 g/kg drug residue group were significantly higher than those in the 0.5 g/kg water extract group (P<0.05).[Conclusion] Epimedium water extract and its residue have therapeutic effect on osteoporosis induced by ovariectomy in female rats. The therapeutic effect of 0.5g/kg epimedium residue is better than that of the same dose of water extract.