| 基于ISSR与ITS-RFLP的花生白绢病菌遗传多样性分析 |
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| 引用本文： | 宋万朵,晏立英,雷永,淮东欣,康彦平,王志慧,姜慧芳,廖伯寿. 基于ISSR与ITS-RFLP的花生白绢病菌遗传多样性分析[J]. 中国油料作物学报, 2021, 43(4): 724-730. DOI: 10.19802/j.issn.1007-9084.2020096 |
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| 作者姓名： | 宋万朵 晏立英 雷永 淮东欣 康彦平 王志慧 姜慧芳 廖伯寿 |
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| 作者单位： | 中国农业科学院油料作物研究所/油料作物生物学与遗传育种重点实验室，武汉430062 |
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| 基金项目： | 国家重点研发计划（2018YFD0201000）；国家自然科学基金（31971981）；现代农业产业技术体系（CRAS-13） |
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| 摘 要： | 本研究利用简单重复序列区间(Inter Simple Sequence Repeat,ISSR)和ITS-RFLP技术,对采自我国12个省市的39个花生白绢病菌菌株进行遗传多样性分析.结果表明:从筛选出的17条ISSR引物共扩增出269条DNA谱带,其中多态性谱带266条,平均多态性百分率为98.9％,UPGMA聚类分...
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| 关 键 词： | 花生白绢病 Sclerotium rolfsii ISSR ITS-RFLP 遗传多样性 |
Genetic diversity of Sclerotium rolfsii infecting groundnut based on ISSR and ITS-RFLP |
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| SONG Wan-duo,YAN Li-ying,LEI Yong,HUAI Dong-xin,KANG Yan-ping,WANG Zhi-hui,JIANG Hui-fang,LIAO Bo-shou. Genetic diversity of Sclerotium rolfsii infecting groundnut based on ISSR and ITS-RFLP[J]. Chinese Journal of Oil Crop Sciences, 2021, 43(4): 724-730. DOI: 10.19802/j.issn.1007-9084.2020096 |
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| Authors: | SONG Wan-duo YAN Li-ying LEI Yong HUAI Dong-xin KANG Yan-ping WANG Zhi-hui JIANG Hui-fang LIAO Bo-shou |
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| Affiliation: | Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China |
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| Abstract: | Inter simple sequence repeat (ISSR) and ITS-RFLP were used to analyze the genetic diversity of 39 Sclerotium rolfsii peanut strains collected from 12 provinces in China. A total of 269 DNA bands were amplified from 17 ISSR primers, 266 bands accounting for 98.9% showed polymorphic among them. These strains were grouped into 4 clusters by UPGMA analysis according to ISSR polymorphic bands. Variation in internal transcribed spacers (ITS) was examined following restriction enzyme digests. Restriction fragment length polymorphisms (RFLPs) were observed in ITS fragments digested by AluⅠ, RsaⅠ, HpaⅡand MboⅠ, and divided 39 strains into seven groups. The results of ISSR and ITS-RFLP showed that there was high level of genetic variation among S.rolfsii strains, and genetic groups did not correlate with geographical origin. |
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| Keywords: | peanut southern stem rot Sclerotium rolfsii ISSR ITS-RFLP genetic diversity |
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